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All Journal HAYATI Journal of Biosciences Media Peternakan
MULYOTO PANGESTU
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Sperm Preservation using Freeze-Drying Method TAKDIR SAILI; MULYOTO PANGESTU; MOHAMAD AGUS SETIADI; SRIHADI AGUNGPRIYONO; MOZES R. TOELIHERE; ARIEF BOEDIONO
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.025 KB) | DOI: 10.4308/hjb.12.1.41

Abstract

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.
PENGARUH WAKTU PEMERAHAN TERHADAP BERAT JENIS DAN BAHAN KERING TANPA LEMAK SUSU SAPI FH DI BBPTU-HPT BATURRADEN Intan Rizkiyani; Susilo Rahardjo; Yanita Viastika; Mulyoto Pangestu
MEDIA PETERNAKAN Vol. 25 No. 1 (2023): Media Peternakan
Publisher : Fakultas Peternakan. Unwiku, Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63859/mp.v25i1.1

Abstract

The research entitled "The Effect of Milking Time on Specific Gravity and Non-Fat Dry Matter of FH Cow's Milk at BPBTU HPT Baturraden" was conducted from 1 to 30 November 2022. The material used in this study was the milk of FH dairy cows at different milking times at BPBTU HPT Baturraden with a sample size of 64 first lactating cows. The method used in this research is a survey method obtained by recording data at BPBTU HPT Baturraden. The data were analyzed using the "z" test. The results of the study proved that the specific gravity content of cow's milk in the morning milking was 3.45%, while the specific gravity content in the afternoon milking was 3.95%. The results of data analysis using the "z" test proved that milking time had no significant effect (P>0.05) on the specific gravity content of cow's milk in BBPTU HPT Baturraden. The non-fat dry matter content of cow's milk in morning milking was 3.14% while the dry matter without fat in cow's milk in the evening milking was 3.11%, the results of data analysis with the "z" test proved that milking time had no significant effect (P> 0.05) to non-fat dry matter of cow's milk. The results of the study concluded that the milking time produced the specific gravity and non-fat dry matter of milk which were relatively the same in the morning and afternoon milking Keyword: FH dairy cows, milk, specific gravity content of milk, milk fat-free dry matter, morning and evening milking.
ANALISIS TINGKAT KEBERHASILAN INSEMINASI BUATAN PADA SAPI SIMMENTAL DAN SAPI PERANAKAN ONGOLE DI KECAMATAN PURWANEGARA KABUPATEN BANJARNEGARA Supranoto; Ngudi Santosa; Fani Evadewi; Tri Sukmaningsih; Mulyoto Pangestu
MEDIA PETERNAKAN Vol. 27 No. 1 (2025): Media Peternakan
Publisher : Fakultas Peternakan. Unwiku, Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63859/mp.v27i1.33

Abstract

This study aims to determine the success rate of artificial insemination of Simmental and Ongole crossbreed cattle in Purwanegara and the factors that influence its success. The results of the study of the success rate of artificial insemination showed that the average value of Secvice Per Conception (S/C) Simmental was 1.394 ± 0.125 and Ongole crossbreed was 1.295 ± 0.117 both approaching 1.00 identifying that each Simmental and Ongole crossbreed cow requires 1 dose of artificial insemination before reaching pregnancy and reflects the productivity level of Simmental and Ongole crossbreed cattle. The results of the study showed that the Conception Rate (CR) value in Simmental cattle was 78% and Ongole crossbreed reached 82%, in the Ongole crossbreed cattle conception rate parameter it was slightly higher when compared to Simmental, this shows that the CR of Ongole crossbreed cattle is better than Simmental cattle. The high Non Return Rate (NRR) value is 78%, while the Ongole crossbreed cattle have a higher non return rate of 82% so that the Ongole crossbreed has a higher NRR than Simmental. The success factors of artificial insemination are the fertility rate at high fertility based on the age and BCS condition of the cattle, the efficiency of the inseminator's work, the duration of reporting until artificial insemination and the quality of nutrition, temperature, climate, weather and cattle maintenance management
Co-Authors Arief Boediono Fani Evadewi Intan Rizkiyani M Agus Setiadi MOZES R. TOELIHERE Ngudi Santosa Srihadi Agungpriyono Supranoto Susilo Rahardjo TAKDIR SAILI Tri Sukmaningsih Yanita Viastika