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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Ilmu Ternak Jurnal Demokrasi TINGKAP Journal of Educational, Health and Community Psychology Abdimas Proceeding Buffalo International Conference Jurnal Sains Peternakan Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) International Journal of Sustainable Tropical Agricultural Sciences Jurnal Sosio Agribisnis COUNS-EDU: The International Journal of Counseling and Education Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY JURNAL ILMIAH PETERNAKAN TERPADU JURNAL PengaMAS Jurnal Peternakan (Jurnal of Animal Science) Musamus Journal of Agribusiness Musamus Journal of Livestock Science Indonesian Journal of Animal Agricultural Science (IJAAS) Jurnal Pengabdian Perikanan Indonesia Jurnal Abdimas Akademika Jurnal Pengabdian Masyarakat Ilmu Terapan (JPMIT) Jurnal Pengabdian Masyarakat Formosa (JPMF) Jurnal Pengabdian Masyarakat Bestari (JPMB) Asian Journal of Community Services (AJCS) Jurnal Pengabdian Nusantara (JPN) Mestaka: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Kolaborasi dan Inovasi IPTEKS Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry BAKIRA: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Community Empowerment Jurnal Pengabdian Masyarakat Jurnal Abdimas Peternakan Abdi Masyarakat (PETAMAS) JIPHO (Jurnal Ilmiah Peternakan Halu Oleo) Jurnal Pengabdian Kepada Masyarakat (JAPIMAS) Jurnal Inovasi Dan Pengabdian Kaa Mieera
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Freeze-Drying Spermatozoa as an Alternative Method for Rescuing Genetic Material of Animal Saili, Takdir
Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 4 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.992 KB) | DOI: 10.14334/wartazoa.v18i4.891

Abstract

Cryopreservation is one of the commonly methods used in spermatozoa preservation in which sperm is frozen and stored in the container of liquid nitrogen. The frozen sperm is still motile after thawing, so it is possible to use it in both artificial insemination and in vitro fertilization to produce an embryo. However, this technique needs a continuous supply of liquid nitrogen and a container as a place to store the frozen sperm. The advanced technique in microinjection has led the possibility of using immotile sperm to fertilize oocyte. Therefore, the sperm preservation method may be simplified because the motility of sperm has not been taken into consideration in fertilization compared to the previous method. Freeze-drying sperm is the proposed method in which the sperm is frozen and sublimated using freeze-drying machine to produce freeze-dried sperm. The freeze-dried sperm might be stored in room temperature or in refrigerator. Several reports have claimed that freeze-dried sperm is not motile but it still has capability to fertilize oocyte, even produces offspring, because its DNA remains intact.   Key words: Cryopreservation, freeze-drying, sperm, oocyte
Working With Plantation Communities: A Reflection of Social Issues Jamayah Saili, Abdul Rahman Saili
Journal of Educational, Health and Community Psychology Vol 2, No 1: April 2013
Publisher : Universitas Ahmad Dahlan

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Abstract

Abstract The plantation industry in Malaysia is aggressively expanding over the past decade driven by global demands for palm oil as a food staple and more recently bio fuels. The rapid growth in the industry is heavily dependent upon high labour and workforce. Such intensity has carried out social impact on the communities including plantation workers, small holders and their dependents. Therefore, this paper will outline what appear to be never ending issues impetus social problems. Ethnic conflict, fighting, gambling, alcohol abuses are only a few issues that call for immediate multi-action plan from all involved stakeholders. Those issues can be the causes as well as the effects. The contributing factors form a chain reaction to the whole social dynamics in the plantation’s climate. The main aim is hence to breach the gap between industries, practitioners, and academicians in order to develop the competencies of the next generation social workers. They can play their roles in tackling the social issues, taking into account the different contexts and environment.   Keywords: Social issues, Plantations, Estate communities, Cause and Effect
Status of ram spermatozoa DNA after freeze-drying process Saili, Takdir; Prasetyaningtyas, wahono Esthi; Setiadi, Mohamad Agus; AgungPriyono, Srihadi; Boediono, Arief
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.586 KB) | DOI: 10.14334/jitv.v11i3.528

Abstract

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay
Rescuing genetic material of unexpectedly die animal Said, Syahruddin; Saili, Takdir
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i2.553

Abstract

Rat cauda epididymides were kept in 1.5-ml centrifuge tubes containing 1 ml milli-Q water or physiological saline (0.9% NaCl) and stored and freezed at -196ºC without cryoprotectant for up to 7 days. After thawing of the cauda epididymis, all spermatozoa were non-motile immediately after collection. All oocytes injected with sperm heads (nuclei) of spermatozoa collected from frozen-thawed cauda epididymis in saline were activated (100%) and gradually decreased (P<0.05) in cauda epididymis frozen in milli-Q water at -196°C (86%), and in control (69%). In activated oocytes, a large proportion of sperm heads had transformed into male pronuclear formation (66-78%). When 1-cell embryos were cultured for 120 h, 7% developed to blastocyst stages. These results indicate that genetic materials of species (at least in the rat) that had unexpectedly die can be saved by a simple method. Key words: ICSI, Sperm Heads, Piezo-Injection, Frozen, Rat
Nilai Panen Cempe (Kid Crop) Kambing Kacang (Capra hircus) di Kabupaten Konawe Utara (Studi Kasus) Saili, Takdir
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014)
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Abstract

One of determined variable to evaluate population growth of livestock in certain area is the number of offspring produced in certain period. On goat and lamb production, productivity could be determined using kid crop variable.  Information related to kid crop, especially kid crop of Kacang goat in Konawe Utara District was limited, hence the research on this issue was need for providing the related data. The objective of this research was to determine kid crop of Kacang goat in Konawe Utara District. The location was sequentially established using stratified and purposive sampling methods, while respondent on the named village was established using counting method.  The results  showed  that  kid  crop of Kacang goat  in Konawe Utara District was 167,71% with 8,61 month of kidding interval and 1,36 kids of litter size,  whereas  mortality was    13,96%.  In  conclusion,  Kambing goat  productivity in Konawe Utara District was still good based on the kid crop variable.     Key word: Kacang goat, kid, kid crop, Konawe Utara.
POTRAIT OF REPRODUCTIVE MANAGEMENT IMPLEMENTATION ON BUFFALO BREEDING IN BOMBANA DISTRICT Nafiu, La Ode; Saili, Takdir
Proceeding Buffalo International Conference 2013
Publisher : Proceeding Buffalo International Conference

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Abstract

Buffalo production is one of the alternative livestock production in attempt to reach meat self-sufficiency program in 2014. Production system of buffalo in Bombana district was still under traditional management in which buffalo are kept in free range system. This study aimed to describe the reproductive management of buffalo breeding in Bombana District. The survey was conducted on the mainland and islands of Bombana district, involved 80 farmers that consisted of 60 farmers in the mainland and 20 farmers in the islands. Some parameters reflected the reproductive management aspects were observed. The results showed that: (1) there was only 50% of farmers who arranged the first mating of their buffalo cows, (2) 66% buffalo cows were first mated at the age of 2-3 years, 22.50% mated over 3 years, and only 11.25% mated under the age of 2 years, (3) 61.25% buffalo cows calving for the first time at the age of 3-4 years and 38.75% over 4 years, (4) 45% had calving interval <1.5 years, 27.50% ranging from 1.5 - 2.0 years and 27.50% was > 2 years, (5) 100% buffalo were natural mating, and (6) 31.25% of farmers did not know the signs of estrus in buffalo.  In conclusion, implementation of reproductive management of buffalo breeding in Bombana was still low. Application of reproductive management by farmers in the mainland areas was better than the islands.
The Effect of Soaking Period on Chemical Quality of Gelatin Derived From Broiler Leg Skin Joka, Elen Rosalia; Hafid, H.; Saili, T.
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

Gelatin is usually used for the healthcare industry, photographic industry, and food industry.Hoee, a the gelati ee still ipoted fo aoad that ot suel halal, so it necessary toproduce our own gelatin. The product of gelatin this experiment used 2%NaOH with differentsoaking periods. The objective of this experiment was to determine the quality base-based gelatinproduced from the skin of broiler legs. Completely randomized design with three treatment ofsoaking periods (1 day, 2 day and 3 day)used in this experiments with three replications. Variablemeasured were pH, water content, fat and inorganic material (ash). Data obtain were analyzed usingvariance analysis and different between treatments were analyzed using multiple range Duncan test.The result showed that periods did not significantly affect the quality of gelatin derived from the skinof broiler legs.
Morfometric Portrait of Swamp Buffalo in Bombana Nafiu, La Ode; Saili, T.; Bain, A.
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

The aim of this study was to find a portrait of body weight and body size of swamp buffalo inmainland and islands of Bombana region.  The parameters observed were: body weight, shoulderand hip height, body length, chest circumference and width of hips. Based on the results of the studyconcluded: (1) the average body weight of buffalo in the islands was 416.9±117 kg, significantlyhigher (P<0.05) compared with the mainland area which averaged only 377.3±100 kg, as well as theaverage hips width of swamp buffalo in island area was significantly higher (P<0.05) than in themainland, (2) portrait of  body measurement of swamp buffalo generally not significantly different(P> 0.05) between the mainland and island regions, except the size of the hips width in the island wassignificantly higher (P<0.05) than the mainland, (3) the main component identifier of the totaldiversity of buffalo body measurements in mainland area was body length (69.30%), while the islandareas was chest circumference (73.50%).Key Words: Buffalo, Body Weight, Body Measurement, Principal Component Analysis
Epididymal Sperm Maturation Level of Bali Cattle Based on The Presence of Cytoplasmic Droplet Sumiarti, Rina; Ba'a, La Ode; Saili, T.
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

Epididymal Sperm Maturation Level of Bali Cattle Based on The Presence ofCytoplasmic Droplet
Variation on Body Weight and Body Dimension of Buffalo in Kabaena Island, Bombana, Southeast Sulawesi Rusdin, Muh.; Nafiu, La Ode; Saili, T.; Rahadi, S.
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

Variation on Body Weight and Body Dimension of Buffalo in Kabaena Island,Bombana, Southeast Sulawesi
Co-Authors . Nurhalim ., Ashar A Hadini, Hairil A. M. Sari A. Napirah Abdul Rizal Abdul Rizal, Abdul Achmad Selamet Aku Afyudi, Bobby Ahmad Dzakir Ahmad Fajar Al Jumiati, Wa Ode Albar, Fahry Anak Agung Istri Sri Wiadnyani Analuddin, La Ode Andi Murlina Andi Murlina Tasse Andini Sulfitrana Andini Sulfitrana Annisa Fathia Abdullah Aprianto . Aprianto Aprianto Arief Boediono Arjuna, Deni Arsiwan Arsiwan Arwin Putra Pratama Asma Bio Kimestri Asmar Hasan Astriana Napirah ASTUTI, MERI Aulia Devinda Afista Auza, Fuji Astuti Awal Maulid Sari Azahrah, Angia Ba'a, La Ode Bahtiar Bahtiar Bana, Sahindomi Basman Basman Boy Arya Sena Budiyanto Darni Darni Dedi Dedi Dedu, La Ode Arfan Deki Zulkarnain Dewi S, Rachmita Dian Agustina Dian Agustina Eki Indrawati Ella Fauzya F. A. Auza Fahyuddin Faradillah, F Febiang Lopulalan Febiang Lopulalan Fitrianingsih Fitrianingsih Fuji Astuty Auza Fuji Astuty Auza Gerhana, Gerhana Gusti Ayu Kade Sutariati H. Hafid Hairil Adzulyatno Hadini Hakim, Muh Haidir Hakim, Muh. Haidir Hamdan Has Harapin Hafid H. Hariono Hariono Hasanuddin Bua Hendhra Sudarno Saputra Hijrawati Hijrawati Hijrawati Hijrawati, Hijrawati I Ketut Mudite Adnyana I Wayam Sura I Wayan Sura Ibrahim, Irianto Ido, Irfan Idrus Salam Ihlas AG, Muhammad Ilahude, Meygi Caesarika Putri Imam Bukhori Irwan Irwan Irwansyah Irwansyah Ismi Wardani Isnaeni, Purnaning Dhian Ita Djuwita Joka, Elen Rosalia Jon Karlino Kadek Juliastini Kesumanengsih, Viny Aprilia Khairiyah, Ummul Kimestry, Asma Bio La Aba La Karimuna La Malesi La Ode Abdul Karim Ishak La Ode Arsad Sani La Ode Ba&#039;a La Ode Ba&#039;a La Ode Baa La Ode Baa La Ode Muhamad Munadi La Ode Nafiu Laode Muhammad Sentot Alibasa Lena Wati Lisran Lisran Lukman Yunus Lukman Yunus, Lukman Luzardin Luzardin M Agus Setiadi Madiki, A Majid Majid Mardianto Mardianto Mas Rizky A.A. Syamsunarno Masrah Masrah Mesrawati Mesrawati Mohammad Ghozali Mohammad Ghozali, Mohammad MOZES R. TOELIHERE Muh. Munadi, La ODe Muh. Rusdin Muhammad Amrullah Pagala MULYOTO PANGESTU Munadi, La Ode Muh Munadi, La Ode Muh. Musram Abadi Natsir Sandiah Nian Anisa Nur Arafah Nur Asma Nur Asni Nur Asyik, Nur Nur Fitry Yanti Nur Santy Asminaya Nur Zahra Suaidi Nurhayanto, Nurhayanto Nuriadin Nuriadin Nuriadin Nuriadin Nylan Aulia Sari Patrecia Dwita Koty Petrus Kune Pratiwi, Arby’in Purnaning Dhian Isnaeni Purnaning Dhian Isnaeni Purnaning Dhian Isnaeni Putra Wiadnyana Putra, Agus Kurniawan Putri, Anindyaningrum Zainal Putu Nara Kusuma Prasanjaya Rahmad Hardiono Rahmat Mulhazaa Rahmiyanti Ismet Inonie Rangga Setiawan Rangga Setiawan Restu Libriani Rijanto, Abdes Rin Orin Ningrum Rin Orin Ningrum Rini Widyastuti Ronny Rachman Noor Rusdin, Muh. Rusdin, Muh. Rusli Badaruddin Rusmiyanto Rusmiyanto Sani Wanti Santi, Nur Sarmin Sitti Aida Adha Taridala Sri Rejeki Sri Yuliana Srihadi Agungpriyono Sumiarti, Rina Sunarti Sunarti Surahmanto Suryanef Suryanef Syahruddin Said Syam Rahadi Syamsuddin Syamsuddin Syamsuddin Syamsuddin Syamsuddin Syamsul Arifin Tamrin Tamrin W. L. Salido Wa Laili Salido Wa Laily Salido Wa Ode Astija Madu Wa Ode Jasnia Wahono Esthi Prasetyaningtyas Widhi Kurniawan Yamin Yaddi Yuni Lestari Yuni Lestari yuniati yuniati Zulkarnaian, Deki