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All Journal HAYATI Journal of Biosciences Jurnal Akademika Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI Jurnal Sains Teh dan Kina
DWI NINGSIH SUSILOWATI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Veterinary

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Journal : Jurnal Akademika Biologi

 1 
KOMUNITAS RHIZOBAKTERIA TANAMAN TEH DENGAN APLIKASI FORMULA BIOIMUNIZER (Chryseobacterium Sp DAN Bacillus Sp) BERDASARKAN GEN 16S rRNA Agnistisya Widaranti; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum; Dwi Ningsih Susilowati
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Commodity of tea (Camellia sinensis) has an important role in the national economy, especially in the field of agro-industries. Based on data obtained from the Directorate General of Plantation, the tea plant productivity declined over the last few years. This is due to the pest attack which result in decreasing the productivity of the tea plant. Increased crop productivity of tea have been done, such as the use of herbicides and insecticides, but until now there is very little effort to increase the production of tea plants by the use of biological agents. Chryseobacterium sp and Bacillus sp are rhizobacteria in tea rhizosphere that could potentially be used as a biocontrol agent (bioimmunizer). The purpose of this study is to determine the community rhizobacteria in tea soil with the addition of bioimmunizer based on 16S rRNA gene using T-RFLP technique. The method used in this research is T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) using enzymes Msp I and Rsa I. The calculated value are relative abundance, Shannon diversity index (H '), evenness index (E), and dominance index. The results of this study indicate that soil samples with the addition of bioimunizer consisting of Arthrobacter sp, Bacillus sp, Actinobacteria, and Chryseobacterium sp.Keyword: T-RFLP, Chryseobacterium sp, Bacillus sp
KOMUNITAS BAKTERI RHIZOSFER TEH MELALUI APLIKASI BIOIMUNIZER (Chryseobacterium sp. dan Alcaligenes sp.) DENGAN METODE TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) Elin Savitri Aviani; Dwi Ningsih Susilowati; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.129 KB)

Abstract

Types of bacteria from the rhizosphere communities actually have been developed as bioimunizer but their consistency is still need further experiment about indigenous bacteria associated with rhizosphere of plants. This study aims to identification on the tea plant rhizosphere bacterial communities which in previous studies has been infected with E. vexans Massee and given bioimunizer as well as the checking existence Chryseobacterium sp. and Alcaligenes sp. The result research showed that communities of bacteria on control samples obtained through culturing Bacillus sp. (51.91%), Acidobacteria bacterium (39.42%) and Actinobacteria sp. (8.66%). Control sample through metagenom obtained Gemmatimonas aurantiaca (5.80%), Bacillus sp. (42.55%), Acidobacteria bacterium (23.45%) and Actinobacteria sp. (28.20%). Communities of bacteria in the samples treated by culturing obtained Gemmatimonas aurantiaca (3.58%), Bacillus sp. (30.76%), Pseudomonas sp. (5.55%) Acidobacteria bacterium (13.94%) and Actinobacteria sp. (46.16%). Communities of bacteria in the samples treated by metagenom found Bacillus sp. (10.66%), Acidobacteria bacterium (4.22%), Actinobacteria sp. (5.48%), Uncultured bacterium (1.49%), Alcaligenes sp. (36.95%) and Chryseobacterium sp. (46.82%). The existence of Alcaligenes sp. and Chryseobacterium sp. show consistency bioimunizer composition is applied. Communities of bacteria on metagenom have diversity and evenness level higher than the culturing approach. Keywords: bacterial communities, T-RFLP, Chryseobacterium sp., Alcaligenes sp.
Co-Authors Agnistisya Widaranti ALFI DATIN ZAUQIAH Eko Pranoto Elin Savitri Aviani Fani Fauziah Hermin Pancasakti Kusumaningrum I Made Samudera I MADE SAMUDRA I Made Sudiana Ifa Manzila LAKSMI AMBARSARI Mieke Rochimi Setiawati Muhammad Faris Fathin Puji Lestari Siti Nur Jannah sri widawati Suliasih Suliasih TEDJA -IMAS Tri Puji Priyatno Yadi Suryadi Yati Rachmiati Yuki Muramatsu