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All Journal HAYATI Journal of Biosciences Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Abdimas Dewantara Indonesian Community Journal
Yanti Lim
Atma Jaya Catholic University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Public Health Social Sciences Veterinary Other

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Inhibition of Urokinase-Type Plasminogen Activator Expression by Macelignan in Porphyromonas gingivalis Supernatant-Induced Human Oral Epithelial Cells . Yanti
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.979 KB) | DOI: 10.4308/hjb.17.1.31

Abstract

 This study was to investigate the effect of macelignan on Porphyromonas gingivalis supernatant-induced uPA expression via regulating mitogen-activated protein kinase (MAPK) and activating protein-1 (AP-1) signaling pathways in human oral epithelial KB cells using casein zymography, Western blotting, reverse transcription-PCR and reporter gene assays. Zymographic analysis of secreted enzymes identified the main caseinolytic band at 54 kDa. Macelignan inhibited the expression of uPA protein and mRNA, as well uPA secretion, in KB cells exposed to P. gingivalis supernatant. Consistent with these findings, macelignan suppressed phosphorylation of p38 and c-Jun N terminal kinase (JNK) in P. gingivalis supernatant-induced KB cells. The levels of c-Fos and phosphorylated c-Jun, which together form AP-1, the transcription factor that is involved in uPA gene expression, were partially reduced by macelignan. Macelignan also blocked P. gingivalis supernatant-induced AP-1 activity in these cells. These results suggest that macelignan decreased P. gingivalis supernatant-induced uPA expression by blocking AP-1 activity, which may be mediated by inhibition of phosphorylation of p38 and JNK in KB cells. Macelignan may potently use for the modulation of periodontal inflammation.
Food Origin Fibrinolytic Enzyme With Multiple Actions Laurentia Stephani; Raymond Rubianto Tjandrawinata; Diana Nur Afifah; Yanti Lim; Wangsa Tirta Ismaya; Maggy Thenawidjaja Suhartono
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (919.422 KB) | DOI: 10.4308/hjb.24.3.124

Abstract

Many health related problems such as cardiovascular diseases are associated with the formation of excessive clot in the blood (thrombus). Approaches in cardiovascular disease treatment are preventing the formation or removing the thrombus. The present thrombolytic agents can be classified as plasminogen activators, fibrinolytic enzyme which directly degrades fibrinogen or fibrin and heparin type which act as thrombin inhibitor. Recently, microbial fibrinolytic enzymes of food origin receive more attention that leads to escalating efforts to explore traditional fermented foods as the natural sources. We have successfully isolated microorganism from Indonesian fermented soybean tofu dregs “Oncom” that secretes fibrinolytic enzyme. The microorganism identified as Stenotrophomonas sp. is unique because most of the reported fibrinolytic microorganism belongs to Bacillus sp. This isolate was found to produce extracellular fibrinolytic enzyme which could degrade fibrinogen and fibrin directly as determined by fibrinogen zymography and fibrin plate methods. More importantly, the 30-kD purified enzymes was found to demonstrate not only fibrin and fibrinogen degradation capabilities, but also acted as thrombin inhibitor as determined using specific substrates for thrombin. This is the first report of a fibrinolytic enzyme that demonstrates additional synergistic activities. This finding accentuates the importance of further development of the enzyme into a powerful agent to treat the thrombus-related disease effectively.
GROWTH AND DEVELOPMENT OF OIL PALM SHOOTS UNDER DIFFERENT LIGHT QUALITIES Helena Patricia Manoh; Yanti Yanti; Nurita Toruan-Mathius
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.0.0.0.903

Abstract

Light is one of the most important factor in plant tissue culture. The growth and development of plant in vitro are affected by light quality. This study examines the influence of different light qualities on the in vitro growth and development of oil palm shoots. Oil palm shoots were cultured in Murashige & Skoog medium under either white fluorescent lamp, white light-emitting diode (LED), red LED, blue LED, combination of red and blue LED, or in darkness. The results show that the growth and development of oil palm shoots are influenced by the light quality. Root initiation and shoot elongation were good under red light, while chlorophyll and sugar content was better produced under white and blue light than red light. In addition to white fluorescent lamp, the combination of red and blue LED resulted in higher growth parameter compared to other light treatments, but the results were not significantly different.
Sosialisasi implikasi minyak jelantah kepada pedagang kantin di Kampus BSD, Universitas Katolik Indonesia Atma Jaya Daru Seto Bagus Anugrah; Adeline Mayvie Wijanarko; Juan David Sinanu; Yanti Yanti; Watumesa Agustina Tan; Krismanto Rabin Chandra
ABDIMAS DEWANTARA Vol 6 No 2 (2023): Article in Press
Publisher : Universitas Sarjanawiyata Tamansiswa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30738/ad.v6i2.15731

Abstract

Minyak jelantah adalah minyak bekas yang diperoleh dari hasil menggoreng makanan. Penggunaan minyak jelantah berulang menyebabkan penurunan kualitas lingkungan dan manusia. Minyak jelantah mengandung zat polifenol dan akrilamida yang meningkatkan risiko kanker dan penyakit lainnya. Selain itu, minyak jelantah juga mempengaruhi kualitas makanan, menyebabkan perubahan rasa dan tekstur makanan. Pembuangan minyak jelantah sembarangan menyebabkan penumpukan pada saluran air dan tempat pembuangan sampah, berdampak negatif pada ekosistem dan organisme di perairan dan tanah. Namun demikian, minyak jelantah masih dapat dimanfaatkan menjadi produk yang lebih bermanfaat. Melihat hal ini, Universitas Katolik Indonesia Atma Jaya (Unika Atma Jaya) Bersama dengan PT Ubah Limbah Indonesia bekerja sama untuk mengedukasi pedagang di kantin Atma Jaya sebagai salah satu bagian masyarakat yang sering menggunakan minyak goreng. Tujuan kegiatan ini adalah untuk meningkatkan pengetahuan pedagang mengenai minyak jelantah serta bagaimana cara mengolahnya menjadi produk lain seperti lilin aromatik. Penyampaian materi dari empat pembicara menunjukkan adanya peningkatan pengetahuan dari pedagang mengenai minyak jelantah, dilihat dari hasil pre-test dan post-test serta analisis Wilcoxon Rank Test. Secara keseluruhan, upaya kolaboratif antara Unika Atma Jaya dan PT Ubah Limbah Indonesia untuk memberikan edukasi kepada pedagang kantin telah berhasil meningkatkan pengetahuan pedagang mengenai potensi dan pengolahan minyak jelantah.   Socialisation of the implications of used cooking oil to the canteen vendors at the BSD Campus of Universitas Katolik Indonesia Atma Jaya   Abstract: Used cooking oil is the residual oil obtained from frying food. Repeated use of used cooking oil leads to a decline in environmental and human quality. Used cooking oil contains polyphenols and acrylamide compounds that elevate cancer risk and other diseases. Furthermore, used cooking oil affects the quality of food, inducing alterations in taste and texture. Improper disposal of used cooking oil results in accumulation within waterways and waste disposal sites, adversely impacting ecosystems and aquatic and terrestrial organisms. Nevertheless, there is potential to repurpose used cooking oil into more beneficial products. Recognizing this, Atma Jaya Catholic University of Indonesia (Unika Atma Jaya), in collaboration with PT Ubah Limbah Indonesia, endeavors to educate vendors at the Atma Jaya canteen, a segment of society that frequently employs cooking oil. This initiative aims to enhance vendors' understanding of used cooking oil and its transformation into alternative products such as scented candles. Delivery of content by four presenters demonstrates an improvement in vendors' comprehension of used cooking oil, evident from pre-test and post-test results and Wilcoxon Rank Test analysis. Overall, the collaborative effort between Unika Atma Jaya and PT Ubah Limbah Indonesia to educate canteen vendors has effectively elevated their knowledge regarding the potential and processing of used cooking oil.
Bioremediation, Biosorption and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 Titania Tjandrawati Nugroho; Putri Nabilah; Nuria Wulandari; Yuana Nurulita; Andi Dahliaty; Yanti Yanti
Jurnal Natur Indonesia Vol 21, No 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.93-99

Abstract

Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T. asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye.  The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.
Pelatihan Pengolahan Mie Basah dan Permen Gummy Berbasis Buah Buni untuk Meningkatkan Asupan Gizi dan Ekonomi Masyarakat Meda Canti; Yanti
I-Com: Indonesian Community Journal Vol 4 No 2 (2024): I-Com: Indonesian Community Journal (Juni 2024)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33379/icom.v4i2.4538

Abstract

Intake of foods rich in nutritional content, such as buni fruit, can be a solution to overcome vitamin C deficiency. However, the use of buni fruit is still limited due to the lack of public knowledge in processing buni fruit. Therefore, training is needed to process buni fruit into products with added value, such as wet noodles and gummy candy. This training aimed to provide insight into public knowledge regarding the benefits and processing of buni fruit into wet noodle products and gummy candy. The method for implementing this activity was training (delivery of material and demonstration). As many as 100% of participants strongly agreed that this training was beneficial and provided insight into the nutritional benefits and processing of buni fruit. Participants succeeded in making wet noodles and gummy candy independently. Continuous assistance is needed for participants who want to become entrepreneurs with processed wet noodle products and gummy candy based on buni fruit.
Co-Authors Adeline Mayvie Wijanarko Andi Dahliaty Anugrah, Daru Seto Bagus Diana Nur Afifah Helena Patricia Manoh Juan David Sinanu Krismanto Rabin Chandra Laurentia Stephani Maggy Thenawidjaja Suhartono Meda Canti Nuria Wulandari Nurita Toruan-Mathius Putri Nabilah Raymond R. Tjandrawinata Tan Watumesa Agustina Titania Tjandrawati Nugroho Wangsa Tirta Ismaya Yuana Nurulita