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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Chimica et Natura Acta Dinamisia: Jurnal Pengabdian Kepada Masyarakat Unri Conference Series: Community Engagement
Yuana Nurulita
Biochemistry Laboratory, Department Of Chemistry, University Of Riau, Pekanbaru 28293
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Engineering Environmental Science Materials Science & Nanotechnology Social Sciences Other

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Optimasilisasi Waktu Fermentasi, Kadar Air dan Konsentrasi Cu2+ pada Produksi Lakase Trichoderma asperellum LBKURCC1 Secara Fermentasi Padat Batang Padi dalam Reaktor Labu Novia Sellyna; Miranti Miranti; Yuana Nurulita; Edy Saputra; Panca Setia Utama; Titania Tjandrawati Nugroho
Chimica et Natura Acta Vol 8, No 1 (2020)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v8.n1.26730

Abstract

Trichoderma asperellum LBKURCC1 adalah galur Trichoderma yang diisolasi dari tanah perkebunan cokelat di Riau yang mampu memproduksi lakase. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin, sekaligus mengoksidasi senyawa fenol. Penelitian ini bertujuan mengoptimasi produksi lakase T. asperellum LBKURCC1 secara fermentasi padat (SSF) menggunakan batang padi sebagai penginduksi lakase, di dalam reaktor labu sederhana. Optimasi parameter fermentasi (waktu fermentasi, kadar air dan konsentrasi Cu2+)dilakukan menggunakan Central Composite Design (CCD) dengan Response Surface Methodology  (RSM). Hasil ANOVA menunjukkan bahwa model quadratik dipilih, dengan persamaan regresi Y= 64,19 - 6,71 X1 + 6,93 X2 - 15,65 X1*X1 - 7,11 X2*X2 - 15,40 X3*X3. Waktu fermentasi, kadar air dan konsentrasi Cu2+ sebagai CuSO4.7H2O ditemukan memiliki efek signifikan (p-value<0,05) terhadap aktivitas lakase yang diproduksi. Kondisi optimal untuk produksi lakase dengan penginduksi batang padi, secara SSF dalam reaktor labu, adalah 7 hari fermentasi, kadar air 67% dan konsentrasi CuSO4.7H2O 0,046 g/L. Aktivitas lakase yang diperoleh pada kondisi optimum adalah 65,3±0,7 mU per gram batang padi. Meskipun hanya meningkatkan aktivitas lakase 2% dari aktivitas pada center point, kondisi optimum tetap membuat proses menjadi lebih ekonomis dan efisien, karena memperpendek waktu produksi dari 8 hari menjadi 7 hari, dan mengurangi konsentrasi penambahan Cu2+.
Metabolit Sekunder Sekresi Jamur Penicillium spp. Isolat Tanah Gambut Riau sebagai Antijamur Candida albicans Yuana Nurulita; Yuharmen Yuharmen; Nuryani Nenci; Ayu Octa Mellani; Titania Tjandarawati Nugroho
Chimica et Natura Acta Vol 8, No 3 (2020)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v8.n3.32452

Abstract

Mikroba dan tanaman merupakan sumber metabolit sekunder yang aktif dieksplorasi potensinya. Laboratorium Enzim, Fermentasi dan Biomolekuler Jurusan Kimia FMIPA Universitas Riau telah mengisolasi beberapa jenis mikroba khususnya jamur dari tanah gambut Hutan Primer Cagar Biosfer Giam Siak Kecil Bukit Batu (GSKBB) Provinsi Riau. Beberapa isolat tersebut adalah jamur Penicillium sp. LBKURCC29 dan LBKURCC30 yang memiliki potensi menghasilkan enzim selulase. Tujuan dari penelitian ini adalah untuk mengeksplorasi potensi lain dari kedua jamur isolat lokat tanah gambut Riau tersebut dalam hal ini potensi sebagai sumber antimikroba, antijamur Candida albicans. Produksi metabolit sekunder antijamur dilakukan dengan cara fermentasi batch dalam media cair yang diinokulasi dengan spora jamur (7×1012 spora untuk 50 mL media) selama 14 hari dengan bantuan rotary shaker kemudian disaring dengan menggunakan kertas saring GF/C. Filtrat kemudian diekstraksi menggunakan etil asetat. Ekstrak etil asetat kemudian diuapkan dan dipekatkan menggunakan rotary evaporator. Ekstrak kasar etil asetat kemudian dilarutkan dengan metanol untuk diuji lebih lanjut. Pada penelitian ini dilakukan karakterisasi ekstrak dengan uji fitokimia, KLT, dan HPLC, serta uji antijamur C. albicans dengan metode difusi cakram. Hasil uji fitokimia menunjukkan bahwa masing-masing ekstrak Penicillium sp. LBKURCC29 dan LBKURCC30 mengandung senyawa terpenoid. Karakterisasi lebih lanjut ekstrak kasar etil asetat diuji KLT dan HPLC menunjukkan pola noda dan puncak yang berbeda. Untuk uji antijamur, hanya ekstrak Penicillium sp. LBKURCC29 yang memiliki aktivitas antijamur terhadap jamur C.albicans pada konsentrasi 5,7 mg/mL dengan diameter zona hambat sebesar 8,05 nm yang merupakan penghambatan 33,78% dibandingkan dengan kontrol positif Ketokonazol® (23,83 mm).
Potensi Metabolit Sekunder dari Trichoderma sp. LBKURCC22 Tanah Gambut Hutan Sekunder Sebagai Antibiotik Nungki Narasswati; Rani Oktavia; Nuryani Nenci; Yum Eryanti; Titania Tjandrawati Nugroho; Yuana Nurulita
Chimica et Natura Acta Vol 5, No 2 (2017)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.778 KB) | DOI: 10.24198/cna.v5.n2.14692

Abstract

Trichoderma sp. LBKURCC22 adalah isolat lokal yang diisolasi dari lahan hutan rawa gambut sekunder. Isolat ini berpotensi menghasilkan metabolit sekunder. Tujuan dari penelitian ini adalah untuk menggali potensi metabolit sekunder dari isolat Trichoderma sp. LBKURCC22 dalam fermentasi batch. Metabolit sekunder diekstraksi dengan etil asetat. Ekstrak diuapkan, kemudian diperoleh ekstrak kasar. Analisis dilakukan dengan uji fitokimia, KLT, dan KCKT. Selanjutnya, aktivitas antibiotik dilakukan terhadap Escherichia coli dan Staphylococcus aureus dengan metode dilusi. Uji fitokimia menunjukkan bahwa ekstrak mengandung alkaloid. Namun, aktivitas antibakteri, senyawa metabolit sekunder dari ekstrak tidak aktif terhadap bakteri-bakteri yang diuji. Hasil penelitian menunjukkan bahwa KLT dengan eluen etil asetat:n-heksana (6:4) setelah disemprot dengan 0,5% p-anisaldehid menunjukkan tidak adanya peptaibol pada noda P1 (Rf :0,78), P2 (Rf 0,65), dan P3 (Rf 0). Analisis KCKT menghasilkan ekstrak mengandung satu puncak pada waktu retensi (tR) 4,115 menit pada 214 nm dan 4,106 menit pada 227 nm. Penelitian ini menunjukkan bahwa isolat Trichoderma sp. LBKURCC22 tidak memproduksi metabolit sekunder yang berpotensi sebagai antibiotik. Perlu dilakukan analisis lebih lanjut untuk mengeksplorasi potensi isolat ini.
Molecular Identification, GC-MS Analysis of Bioactive Compounds and Antimicrobial Activity of Thermophilic Bacteria Derived from West Sumatra Hot-Spring Indonesia Zona Octarya; Titania Tjandrawati Nugroho; Yuana Nurulita; Saryono
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.549-561

Abstract

Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One of them is as a source of antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing bioactive compounds and their potential as antimicrobial agents. Thermophilic bacteria with the code LBKURCC were taken from the collection of the Biochemistry Laboratory of the University of Riau. Forty-four purified strains of thermophilic bacteria were tested for antimicrobial ability. These thermophilic bacteria were taken from hot springs located in the Sumatra provinces of West Sumatra and Riau. Strain LBKURCC218 isolated from Rimbo Panti hot springs in West Sumatra was chosen to further investigate antimicrobials production. Isolates of hot spring bacteria that produced the highest antimicrobial were identified by comparing the similarity of the 16S rRNA gene sequences. BLAST result and phylogenetic tree showed that the selected thermophilic bacterial strain was similar to Bacillus paramicoides with the similarity index of 99.93%. Analysis of bioactive compounds of the ethyl acetate extract of liquid cultures of B. paramycoides LBKURCC218 showed the best producer of antimicrobial compounds compared to other isolates. The most identified compounds from the ethyl acetate extract were Dodecanoic acid, representing 23.62% of the total compounds, followed by 11-Dodecanoic acid at 17.84%. Ethyl acetate extract of B. paramycoides LBKURCC218 has a high inhibition zone against Escherichia coli, Staphylococcus aureus, and Candida albicans.
Penerapan teknologi biopori vertikal dengan memanfaatkan larva Black Soldier Fly sebagai pengurai Andi Dahliaty; Halida Sophia; Yuana Nurulita; Sri Helianty
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.207-214

Abstract

The biopore infiltration hole is a hole with a diameter of 10 cm - 30 cm with a depth of 30 cm - 100 cm which is added with organic waste and will help soil biota form a biopore hole so that it can trap the air flowing around it. Black Soldier Fly larvae are able to accelerate composting and ordering of pores in the biopore infiltration holes. Perumahan Putri Indah Kulim RT 02 RW 12 Kelurahan Mentangor rarely finds open land semisization and house widening which causes a reduction in the surface of ground air infiltration so that during the rainy season puddles easily occur and during the dry season it reduces groundwater reserves. This activity aims to provide knowledge and insight into the community about biopore infiltration holes by utilizing Black Soldier Fly larvae and inviting the public to apply this technology. This service activity begins with the socialization of the biopore infiltration holes, then introduces the tools and materials as well as direct practice in the field. The results of this activity had a positive influence on the community and many community members who wanted to apply the biopore infiltration hole technology using Black Soldier Fly larvae. This is the first step in creating a clean and healthy environment, especially in Putri Indah Kulim Housing RT 02 RW 12 Mentangor Village.
Pengenalan Bunga Telang (Clitoria ternatea L.) di Desa Wisata Alam Sungai Masjid Kabupaten Dumai sebagai upaya membantu peningkatan Hospitality Ninik Nihayatul Wahibah; Delita Zul; Atria Martina; Yulminarti Yulminarti; Ennie Chahyadi; Yuana Nurulita
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.531-535

Abstract

Kelompok Sadar Wisata or Tourism Awareness Group (POKDARWIS) Desa Wisata Sungai Masjid Kabupaten Dumai have been improving their rural tourism. One of their services for tourists or guests is providing welcome drinks. Therefore, the objective of our program was to introduce butterfly pea, how to plant and how to utilize the flowers particularly for improving hospitality such as welcome drink and welcome kit. Butterfly pea (Clitoria ternatea L.) plants are known relatively easy to grow and possess chemical properties good for human health. We delivered our program to POKDARWIS Desa Wisata Sungai Masjid ss our partner through the oral presentation and demonstrated video. We also gave seeds and seedlings of butterfly pea to the participants. Our partners did not know about butterfly pea flower before participating in our program. They showed their enthusiasm and curiosity, particularly during the discussion session. Results of the questionnaire indicated that our program was useful to enhance their knowledge and provide inspiration to improve their life. One week later, after the training, the POKDARWIS had their program which adopted our program such as sowing and planting butterfly pea seeds and seedlings. Based on the results, we conclude that we have conveyed our program successfully and inspired our partners to produce butterfly pea-based food and beverages.
Value Added Eco Enzyme Sebagai Sabun Antiseptik Poppy Nurmayanti M; Yuana Nurulita; Jeni Wardi; Annisa Wulandari; Eka Lestari; Nesya Billa Sausan; Meisy Zahrati Afifah; Meisy Dwi Intan Sari; Niko Andriansyah; Rahmita Khairani Asrar; Yusma Dani; Eliza Khoirunisa; Rialdy
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 5 (2022): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v6i5.10997

Abstract

The purpose of community service is to educate the public in processing eco enzymes into products that have added value, as antiseptic soap. This activity was carried out as an effort to support the government's program in maintaining health through the movement of washing hands with soap. The activities method is training and mentoring carried out at the Bank Sampah Agrowisata, Rumbai District, Pekanbaru City. This activity includes two stages. First, the team conducted training and assistance in making eco-enzymes from citrun fruit peels, water, and palm sugar. The organic waste fermentation process is carried out for one month. Second, the team provided training and assistance in making antiseptic soap from eco enzyme. The results the effectiveness of antibacteria test at the Chemistry Laboratory FMIPA, Riau University showed that eco-enzyme that has been processed into soap tends to be more effective at killing bacteria than eco-enzyme. These results are expected to improve the community's economy to support zero waste and improve the skills and entrepreneurial of the community.
Pemanfaatan Bunga Telang (Clitoria ternatea L.) sebagai Teh yang bermanfaat bagi kesehatan masyarakat di Kampung Eduwisata Alam Sungai Masjid Kota Dumai Ninik Nihayatul Wahibah; Delita Zul; Atria Martina; Yulminarti Yulminarti; Yuana Nurulita; Ennie Cahyadi; Ahmad Huesean; Muhammad Rizky Darmawan; Ikhlasul Febrianto; Tania Aisyah Rinaldi; Nurkhasanah Putri Rakhman
Unri Conference Series: Community Engagement Vol 4 (2022): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.4.144-148

Abstract

Butterfly pea (Clitoria ternatea L.) known as ‘bunga telang’ is perennial plant originated from Indonesia. Butterfly pea flowers are edible flowers known to have active compounds such as antioxidants, anti-inflammatory, antibacterial, anti-cancer. These plants are grown at a lot of house-yard in Kampung Eduwisata Sungai Alam and are commonly used for natural dye for food and beverage. Therefore, objective of our programme was to introduce simple method for producing butterfly flower tea to Pokdarwis (Kelompok Sadar Wisata) Kampung Eduwisata Sungai Masjid. Result of the programme indicated that the participants were really enthusiastic involving in this training. Based on result of the questionnaire reveal that most of participants have positive perception to the training that contributing to improve their knowledge and skills in processing butterfly pea flowers. Percentage of participants who understand how to process the flower increase from 20% to 100% after the training. In addition, butterfly tea can be a commercially distributed that may improve economic level of the Sungai Masjid community. Furthermore, our programme motivates the community to take a part in conservation of natural resources.
Identifikasi Metabolit Sekunder Sekresi Jamur Lokal Tanah Gambut Riau Penicillium sp. LBKURCC34 Sebagai Antimikroba Yuana Nurulita; Yuharmen Yuharmen; Annisa Fitri; Iin Evita Sari; Dian Novita Sary; Titania Tjandrawati Nugroho
Chimica et Natura Acta Vol 10, No 3 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n3.45994

Abstract

Penicillium sp. LBKURCC34 adalah jamur yang diisolasi dari tanah gambut hutan primer di Giam Siak Kecil Bukit Batu (GSKBB), Cagar Biosfer di Provinsi Riau. Tujuan dari penelitian ini adalah untuk mengeksplorasi potensinya sebagai penghasil antimikroba. Metode dilakukan melalui produksi metabolit sekunder dalam media cair, ekstraksi dalam larutan etil asetat, dan pemisahan ekstrak kasar dengan Kromatografi Kolom (CC). Ekstrak kasar dan fraksi diidentifikasi dengan KLT dan HPLC dan diuji aktivitas antimikrobanya dengan metode difusi cakram. Untuk mengidentifikasi senyawa aktif dilakukan uji fitokimia dan metode KLT-Bioautografi. Produksi tiga liter media menghasilkan 0,4 gram (0,0133% b/v) ekstrak etil asetat yang diidentifikasi sebagai terpenoid dan fenolat; dan mengandung senyawa semipolar berdasarkan uji KLT dan HPLC. Hasil KLT setelah disemprot dengan p-anisaldehida 0,5% menunjukkan adanya senyawa peptaibol (peptida). Ekstrak etil asetat dapat menghambat pertumbuhan kelima mikroba patogen yang diuji namun hanya 11-45% dibandingkan dengan kontrol positif. Uji bioautografi terhadap Escherichia coli menunjukkan noda aktif zona bening pada Rf sebesar 0,67. Sayangnya, dari Fraksi 1-5 yang terdapat noda dengan Rf 0,67 masih belum menunjukkan aktivitas berarti. Penyelidikan lebih lanjut diperlukan untuk mengidentifikasi struktur senyawa aktif dari isolat lokal ini.
Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation Citra Hardiyanti; Khairullinas Khairullinas; Jeky Sasemar Lumban; Titania Tjandrawati Nugroho; Yuana Nurulita
Jurnal Kimia Sains dan Aplikasi Vol 23, No 3 (2020): Volume 23 Issue 3 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2803.553 KB) | DOI: 10.14710/jksa.23.3.89-95

Abstract

An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is Penicillium sp. LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added. Staphylococcus aureus was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of S. aureus biotic elicitor extended the log phase growth of the fungus Penicillium sp. LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3rd day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.
Co-Authors Adel Zamri Ahmad Huesean Ali Djamhuri Andi Dahliaty Andi Dahliaty Annisa Fitri Annisa Wulandari Atria Martina Ayu Octa Mellani Citra Hardiyanti Delita Zul Dian Novita Sary Eka Lestari Eliza Khoirunisa Ennie Cahyadi ENNIE CHAHYADI, ENNIE Halida Sophia Iin Evita Sari Ikhlasul Febrianto Jeky Sasemar Lumban Jeni Wardi Jufrizal Syahri Khairullinas Khairullinas Meisy Dwi Intan Sari Meisy Zahrati Afifah Miranti Miranti Muhammad Rizky Darmawan Nesya Billa Sausan Niko Andriansyah Ninik Nihayatul Wahibah Novia Sellyna Nungki Narasswati Nuria Wulandari Nurkhasanah Putri Rakhman Nurwijayanti Nuryani Nenci Nuryani Nenci Poppy Nurmayanti Putri Nabilah Rahmita Khairani Asrar Rani Oktavia Rialdy Rudi Hendra Saryono Sri Helianty Tania Aisyah Rinaldi Titania Tjandrawati Nugroho Utama, Panca Setia Yanti Lim Yuharmen - Yuharmen Yuharmen Yuharmen Yuharmen YULMINARTI, YULMINARTI Yum Eryanti Yum Eryanti Yusma Dani Zona Octarya