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All Journal HAYATI Journal of Biosciences Jurnal Rekayasa Proses MEDIA MEDIKA INDONESIANA Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Jurnal Rekayasa Proses
Ratna Asmah Susidarti
1Department Of Pharmaceutical Chemistry, Faculty Of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. 2Cancer Chemoprevention Research Center, Faculty Of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
8-Methoxycapnolactone and stigmasterol From Micromelum minutum Ratna Asmah Susidarti; Marwadi Rahmani; Abdul Manaf Ali; M. Aspollah Sukari; Hazar B.M. Ismail; Julius Kulip; Peter G. Waterman
Indonesian Journal of Pharmacy Vol 18 No 2, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.688 KB) | DOI: 10.14499/indonesianjpharm0iss0pp105-109

Abstract

The coumarin, 8-methoxycapnolactone and stigmasterol were isolated from the leaves of Micromelum minutum (Rutaceae) which collected from Sepilok, Sabah, Malaysia and their structures were characterized by spectroscopic methods.Key words: Micromelum minutum; Coumarin; 8-Methoxycapnolactone, Stigmasterol
Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity Maulita Cut Nuria; Wahyono .; Ratna Asmah Susidarti
Indonesian Journal of Pharmacy Vol 22 No 1, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.188 KB) | DOI: 10.14499/indonesianjpharm0iss0pp1-8

Abstract

Isolation and  identification  of  kaempferol  from  jangkang  (Homalocladium platycladum (F.  Muell)  Bailey)  leaves  and  its  activity  against  B.  subtilis ATCC 9466 dan S. typhi ATCC 1408 has been done. The dried ground leaves (300.04 gram)  were  macerated  with  petroleum  ether,  chloroform  and  methanol respectively.  The  methanol  extract  was  the  most  active  extract  against  both bacteria.  Further  separations  of  this  extract  using preparative  thin  layer chromatography  yielded  flavonoid  glycoside.  Its  aglicon  was  identified  as kaempferol  based  on  its  spectroscopic  data  (MS,  IR, and  UV-Vis)  and  UV spectrum  (densitometry).  This  compound  shows  iradical  zone  of  6.62  mm (Bacillus  subtilis)  and  6.27  mm  (Salmonella  typhi)  at  concentration  of  200 µg/disk.Key words : kaempferol, jangkang, glycoside, aglicon
The Effect of Brazilin from Caesalpinia sappan on Cell Cycle and Modulation and Cell Senescence in T47D cells Riris Jenie; Sri Handayani; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp84

Abstract

Ethanolic extract and brazilein-containing fraction of Caesalpinia sappan L., has been reported to inhibit cell proliferation in T47D (ER+ PR+/- cell, Luminal A subtype model). The Luminal A subtype is the most common subtype of breast cancer in Indonesian women. In this study, we explored the activity of the reduced form of brazilein, i.e. brazilin, in T47D cells proliferation and the mechanism that involved. The cytotoxicity activity of brazilin was observed using MTT assay. While the cell cycle modulation analysis was done by using flowcytometry, and the senescence assay was observed using S-A-β-galactosidase assay. The results showed that brazilin inhibited cell growth in a dose-dependent manner with an IC50 value of 50μM (or 14.3μg/mL). That was higher than a brazilein-containing fraction, which was reported previously by our group to have an IC50 value of 68μg/mL against the same cell. Cell cycle analysis showed that cells treated with brazilin were accumulated at the G2/M phase in a dose-dependent manner. Furthermore, cells treated with a combination of brazilin and doxorubicin was accumulated at the G2/M phase and sub G1 phase. Cells accumulation at sub G1 phase indicates that the cells undergo apoptosis. Our data of S-A-β-galactosidase assay showed that cells treated with 1/4IC50, 1/2IC50, and IC50 brazilin had lower senescent cells compared to the untreated cells. The morphology of cells treated with IC50 (50μM) brazilin changed. The cells shape became rounded, cells were shrinkage and detached from the well plate, indicating that cells may undergo apoptosis. These results suggested that brazilin was cytotoxic towards T47D cells and its combination with dox potentially induced apoptosis and decreased cell senescence. The ability of brazilin to decrease cell senescence provides new insight of utilization of C. sappan or its constituents, particularly brazilin, as anti-ageing.
Co-Authors Abdul Manaf Ali Akhmad Darmawan Andi Eko Wibowo Ari Sudarmanto Bambang Tri Purwanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Sulistyowati Endang Lukitaningsih Fitria Rahmi Gunardi Gunardi Hari Susanti Hazar B.M. Ismail Hilda Ismail, Hilda Ika Puspita Sari Indah Purwantini Indah Tri Nugraha Jenie, Riris Istighfari Julius Kulip Kurnianto, Rifki Wahyu M. Aspollah Sukari Marwadi Rahmani Maulita Cut Nuria Muhammad Fahrurrozi Mustofa Mustofa Ni Putu Linda Laksmiani Nur Ismiyati Peter G. Waterman Pudjono Pudjono Puspa Dewi Narrij Lotulung Riris Jenie Rochmadi Rochmadi Sismindari Sismindari Siti Musinah Sri Handayani Sri Handayani Sri Handayani Subagus Wahyuono Ulfatul Husnaa Wahyono . Wahyono Wahyono Warsinah Warsinah Yuli Puspito Rini Zalinar Udin