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Pengaruh Penambahan Dedak Padi dan Lactobacillus plantarum 1BL-2 dalam Pembuatan Silase Rumput Gajah (Pennisetum purpureum) R Ridwan; S Ratnakomala; G Kartina; Y Widyastuti
Media Peternakan Vol. 28 No. 3 (2005): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Silages making is a solution to overcome the shortage or excessive of forages in Indonesia. The objective of this study was to evaluate the effect of rice bran as carbohydrate source and Lactobacillus plantarum 1BL-2 addition on the quality of napier grass (Pennisetum purpureum) silages. The concentrations of rice bran and Lactobacillus plantarum 1BL-2 added were 0%,1%, 3% and 5 % (w/w) and incubated for 30 days. The results showed that the addition of 1%-5% rice bran significantly affected (P

Fermentasi Silase dan Manfaat Probiotik Silase bagi Ruminansia Y Widyastuti
Media Peternakan Vol. 31 No. 3 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Forage conservation has long been a part of the agricultural scene in some countries in the world. Ensilage is a preservation method for moist forages that is based on natural lactic acid fermentation under anaerobic conditions. There are six phases which occur during ensilage, storage and feed-out of the fermented forages. The technology of silage making is not popular in Indonesia, although ensilage may successfully occurs in tropical area including Indonesia. The reason may be due to limited information available regarding ensilage for the farmers. This review covered silage fermentation process and probiotics effect of feeding silage to the ruminants. The role of lactic acid bacteria is very important both from the preservation and antimicrobial points of view. Key words: lactic acid bacteria, fermentation, silage, probiotics

Isolasi dan Karakterisasi Enzim Karbohidrase Cairan Rumen Sapi Asal Rumah Potong Hewan A Budiansyah; B Resmi; K G Wiryawan; D M. T. Soehartono; Y Widyastuti; N Ramli
Media Peternakan Vol. 33 No. 1 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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The aims of this experiment were to identify carbohydrases in rumen liquor of cattle, and to determine optimum temperature and pH of enzymes, as well as to evaluate the effect of metal ions and chemical substances on enzymes activity. Optimum precipitation of the enzymes from local and imported cattle was reached at the concentration of 60% and 70% of ammonium sulphate, respectively. The enzymes (xylanase, mannanase, and amylase) had optimum temperature in the range of 50-60 °C, except for cellulase from imported cattle which had optimum temperature at 39 oC, optimum pH of the enzymes were 6-7 except for cellulase was at pH 4. The enzymes mostly needed methal ions as activators. EDTA and β-mercaptoethanol inhibited activity of cellulase from rumen liquor of local cattle, while enzymes from rumen liquor of imported cattle EDTA inhibited xylanase activity and β-mercaptoethanol inhibited mannanase and amylase activity. Activity of cellulase in rumen liquor of local cattle was higher than those of imported cattle. It is concluded that rumen liquor of imported and local cattles from abbatoir contained cellulase, xylanase, mannase and amylase that most of them had optimum temperature and pH at 50-60 oC and 6-7, respectively, and they needed methal ions as activators. Key words: cattle, rumen liquor, abattoir, enzymes, characterization

Quality of Vegetable Waste Silages Treated with Various Carbohydrate Sources W D Astuti; Y Widyastuti; R Ridwan; E Yetti
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this research was to evaluate the quality of vegetable waste silages, using rice bran, onggok (cassava flour waste) and pollard as carbohydrate sources. Vegetable waste was collected from local traditional market, consisted of corn husk, chinese cabbage and cabbage. Research was held in randomized block design consisted of six treatments with 3 replications. Treatments were (T1) vegetable waste + rice bran, (T2) vegetable waste + rice bran + rice straw, (T3) vegetable waste + onggok, (T4) vegetable waste + onggok + rice straw, (T5) vegetable waste + pollard, (T6) vegetable waste + pollard + rice straw. Lactobacillus plantarum 1A-2 was used as innoculant. The quality of silages was evaluated by measuring pH, temperature, population of lactic acid bacteria and lactic acid production. Nutrient characteristic was determined by proximate and fiber analysis. Results showed that pH of silages were not affected by treatments, but silage treated with rice bran, with or without rice straw addition, had higher temperature compared with others (29 oC or 28.3 oC). The highest population of lactic acid bacteria (1.65 x 109 cfu/g) was found in silage using rice straw and onggok (T4), but the highest lactic acid production (0.41%) was measured in silage using rice straw and rice bran (T2). In general, the use of rice bran as carbohydrate sources gave the highest lactic acid production followed by pollard and onggok. Different carbohydrate source gave different nutrients characteristic. Although the result was not significantly different, silage with highest protein content was measured in silage with pollard as carbohydrate source, followed with rice bran and onggok. The result showed that all carbohydrate sources used in this experiment can be used as silage ingredient resulting in good vegetable waste silage.

In Vitro Digestibility of Ration Containing Different Level of Palm Oil Frond Fermented with Phanerochaetae chrysosporium A Imsya; E B Laconi; K G Wiryawan; Y Widyastuti
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Phanerochaete chrysosporium was widely used to delignify agricultural waste product and improve biodegradation of the substrate as animal feed. The experiment was carried out to increase the use of palm oil fronds as a substitute material for napier grass through biodegradation process with P. chysosporium. A completely randomized design with four treatments and four replications was used. The treatments were ration containing 60% napier grass (R1), ration containing 40% napier grass and 20% fermented palm oil frond (R2), ration containing 20% napier grass and 40% fermented palm oil frond (R3), ration containing 60% fermented palm oil frond (R4). Fourty percent concentrate was included in all treatment rations. Parameters measured were in vitro digestibilities of dry matter, organic matter, crude fiber, NDF, ADF, NH3, TVFA, and ruminal cellulolitic bacteria. Results showed that increasing level of fermented palm oil frond in the ration reduced (P<0.05) digestibilities of dry matter, organic matter, crude fiber, NDF, ADF, N-NH3, TVFA concentration and number of ruminal cellulolytic bacteria. It is concluded that fermentation of palm oil frondwith P. chysosporium decrease lignin content by 47.79%, but increasing the fermented palm oil frond in the ration reduces nutrient digestibilities, N-NH3 and TVFA concentrations and rumen cellulolytic bacteria counts. Fermented palm oil frond up to 40% could be used as a substitute for forages in ruminant rations.

Rumen Fermentation and Milk Quality of Dairy Cows Fed Complete Feed Silages K Komalasari; D A Astuti; Y Widyastuti; R Ridwan
Media Peternakan Vol. 37 No. 1 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was conducted to evaluate the rumen fermentation and milk quality of Friesian Holstein (FH) cows given complete feed silages during lactation. Twelve FH cows in 5th mo lactation were offered four dietary treatments in a completely randomized design with three replications. The treatments were, control diet (NS) containing 50% concentrate, 45% elephant grass and 5% sun flower meal; grass complete feed silage (GS) containing 50% concentrate, 45% elephant grass and 5% sunflower meal; rice straw complete feed silage (RSS) containing 50% concentrate, 30% elephant grass, 15% rice straw and 5% sunflower and palm oil frond complete feed silage (PKS) containing 50% concentrate, 30% elephant grass, 15% palm oil frond, and 5% sunflower meal. Ensilage was done with addition of Lactobacillus plantarum 1A-2 and cellulase enzyme. Analysis of variance and Duncan test were applied to compare the different among the means of treatments. Complete feed silages had range of pH between 3.89-4.44, temperature of 28.0-29.67 oC and lactic acid bacteria of 0.54-1.50 x 108 cfu/g. Crude protein intake of RSS was the highest among treatments. Acetate concentration in rumen liquor was more than 70%. Milk yield and protein were not different among treatments. GS gave the highest milk fat (5.66%). The conclusion was that both complete feed silages, using rice straw or palm oil frond can be used as alternative rations for lactating dairy cows. Key words: complete feed, silage, Friesian Holstein, palm oil frond, rice straw

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