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Isolasi dan Karakterisasi Enzim Karbohidrase Cairan Rumen Sapi Asal Rumah Potong Hewan A Budiansyah; B Resmi; K G Wiryawan; D M. T. Soehartono; Y Widyastuti; N Ramli
Media Peternakan Vol. 33 No. 1 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.533 KB)

The aims of this experiment were to identify carbohydrases in rumen liquor of cattle, and to determine optimum temperature and pH of enzymes, as well as to evaluate the effect of metal ions and chemical substances on enzymes activity. Optimum precipitation of the enzymes from local and imported cattle was reached at the concentration of 60% and 70% of ammonium sulphate, respectively. The enzymes (xylanase, mannanase, and amylase) had optimum temperature in the range of 50-60 °C, except for cellulase from imported cattle which had optimum temperature at 39 oC, optimum pH of the enzymes were 6-7 except for cellulase was at pH 4. The enzymes mostly needed methal ions as activators. EDTA and β-mercaptoethanol inhibited activity of cellulase from rumen liquor of local cattle, while enzymes from rumen liquor of imported cattle EDTA inhibited xylanase activity and β-mercaptoethanol inhibited mannanase and amylase activity. Activity of cellulase in rumen liquor of local cattle was higher than those of imported cattle. It is concluded that rumen liquor of imported and local cattles from abbatoir contained cellulase, xylanase, mannase and amylase that most of them had optimum temperature and pH at 50-60 oC and 6-7, respectively, and they needed methal ions as activators. Key words: cattle, rumen liquor, abattoir, enzymes, characterization

Penggunaan Ekstrak Metanol Bawang Putih (Allium sativum) Sebagai Feed Additive Terhadap Kualitas Karkas Ayam Broiler U. Haroen; A. Budiansyah
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 21, No 2 (2019): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.21.2.109-121.2019

Performance of Broiler Chickens Fed Diet Added with Buffaloes Rumen Fluid Enzymes from Slaughterhouses Ucop Haroen; Agus Budiansyah; Noperdiman Noperdiman; Harnita Harnita; Jusalia Jusalia
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.41880

This study was conducted to evaluate the effect of addition of rumen fluid enzymes of buffaloes from slaughterhouse in diet on feed intake, slaughter weight, daily weight gain, feed conversion ratio, absolute carcass weight, relative carcass weight, and digestive organs of broiler. Materials used was two hundred unsexed 1 day old chicks of broilers. The study used a completely randomized design with five treatments and four replications consisted of 10 birds each replication. Treatment groups were as follows P0 = diet without addition of rumen fluid enzymes (control); P1 = diet with addition of rumen fluid enzymes of 0.75%; P2 = diet with addition of rumen fluid enzymes of 1.5%; P3= diet with addition of rumen fluid enzymes of 2.25%; P4= diet with addition of rumen fluid enzymes of 3%. The variables observed were feed intake, slaughter weight, daily weight gain, feed conversion ratio, absolute carcass weight, relative carcass weight, and digestive organs. Results of the study showed that the treatments gave a non-significant different (P>0.05) on feed intake, slaughter weight, daily weight gain, feed conversion ratio, absolute carcass weight, relative carcass weight, and digestive organs of broiler chicken. It could be concluded that the use of rumen fluid enzymes of buffaloes in the diets up to 3% level did not increase performance and digestive organs of broiler chicken.

Performance of Kerinci Ducks Treated by Cattle Rumen-Fluid Supernatant Addition as Source of Crude Enzyme in Rations A. Budiansyah; Resmi Resmi; Filawati Filawati; U. Haroen
Tropical Animal Science Journal Vol. 43 No. 2 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this study was to determine the effect of varying levels of cattle rumen fluid obtained from abattoirs as sources of a crude enzyme on growth performances and carcass characteristics of Kerinci ducks fed rations based on local feed ingredients. The research was carried out using 140 one-week-old male Kerinci ducks. The experiment used a randomized block design consisted of 5 treatments, each with 4 replicate groups and was blocked based on body weight, while each cage unit consisted of 7 ducks. The treatments applied were R1= 0 mL RFS/kg ration (control), R2= R1 + 22 mL RFS/kg ration, R3= R1 + 44 mL RFS/kg ration, R4= R1 + 66 mL RFS/kg ration, and R5= R1 + 88 mL RFS/kg ration. The experimental ducks were fed the experimental ration for 7 weeks and fed ad libitum according to the treatment. At the end of the 7th weeks of treatment, two ducks from each cage were slaughtered and utilized for analysis of carcass and digestive organs parameters. The results showed that the addition of RFS up to a level of 88 mL/kg ration had no significant effect (p>0.05) on feed consumption, feed conversion, carcass weight, and digestive-organ weight. Still, it had significant effects on body weight gain and final body weight. The highest body weight gain and final body weight were achieved with the addition of RFS at the level of 88 mL/kg ration. It can be concluded that the addition of cattle rumen-fluid supernatant as a source of crude enzymes at the level of 88 mL/kg ration improved the growth performance of local Kerinci ducks.

The Use of Hydrolyzed Palm Kernel Cake After Addition by Buffalo Rumen Fluid Enzymes on Growth Performances and Relatively Organ Weight of Broilers Agus Budiansyah; Ucop Haroen; Anie Insulistyowati; Syafwan Syafwan
Buletin Peternakan Vol 46, No 3 (2022): BULETIN PETERNAKAN VOL. 46 (3) AUGUST 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i3.75010

This study aims to utilize a sufficient amount of palm kernel cake (PKC) and buffalo rumen fluid as a source of enzymes to improve the quality of PKC that can be used in broiler feed. The study was conducted in two stages. The first stage was to test the level of buffalo rumen fluid enzyme at various doses (0.0%, 0.75%, 1.5%, 2.25%, and 3.0% (v/w) in PKC incubation to measure dry matter (DM), organic matter (OM), crude fiber (CF), and dissolved glucose total (DGT) contents as hydrolyzed PKC. The second stage was to determine the usage level of hydrolyzed PKC in broiler feed. A total of 288 DOC MB202 strains were randomly allocated to one of five treatments with four replicated cages of 12 birds in a completely randomized design. Treatments were the various level of hydrolyzed PKC as following: 0%, 6%, 12%, 18%, 24%, and 30%. The variables were feed consumption, body weight gain (BWG), feed conversion ratio (FCR), final body weight (FBW), carcass, and digestive organs weight. The level of buffalo rumen fluid enzymes had a significant effect on DM, CF, and DGT content, while it was not significant on OM. The optimum level was 2.25% and it was used in the second stage. The use of hydrolyzed PKC up to 18% was not different (P>0.05) in BWG, FCR, and FBW compared to controls. The use of hydrolyzed PKC up to 24% decreased BWG, FCR, and FBW, but feed consumption did not differ (P>0.05) compared to control. Carcass and digestive organ weight were not affected (P>0.05) by the treatment. It can be concluded that the incubated buffalo rumen fluid enzymes at a level of 2.25% could improve the quality of PKC. The use of hydrolyzed PKC could be applied up to 18% without affecting the performance of broilers

Indeks Keanekaragaman Hasil Tangkapan Jala (Cast net) di Sungai Tabir Kelurahan Mampun Kecamatan Tabir Kabupaten Merangin Bibit Bibit; Lisna Lisna; Agus Budiansyah; Nelwida Nelwida; Eko Wiyanto; Fauzan Ramadhan
Ilmu Perairan (Aquatic Science) Vol 10, No 3 (2022): November
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.10.3.p.185-189

Penelitian ini dilaksanakan pada tanggal 15 mei 2022 sampai 19 juli 2022 di Sungai Tabir Kelurahan Mampun Kecamatan Tabir Kabupaten Merangin. Metode yang digunakan dalam penelitian ini yaitu metode survei. Analisis data yang digunakan adalah Komposisi Jenis, Indeks Keanekaragaman, Indeks Keseragaman dan Indeks Dominansi. Hasil tangkapan Jala selama 15 hari penelitian di Sungai Tabir yaitu Ikan Seluang Rasbora argyotaenia 234 ekor dengan rata-rata per hari 15,6 ekor, Ikan Senggiring Mystus Sp. 119 ekor dengan rata-rata 7,93 ekor, Ikan Kapiat Barbonymus schwanenfeldii 89 ekor dengan rata-rata 5,93 ekor, Ikan Nilem Osteochilus vittatus 77 ekor dengan rata-rata 5,13 ekor dan Ikan Beterung Pristolepis fasciata 55 ekor dengan rata-rata 3,67 ekor. Hasil indeks keanekaragaman 1,47, indeks keseragamannya 0,91 dan indeks dominansi 0,26. Dapat diambil kesimpulan bahwa Indeks Keanekaragaman Sedang, Indeks keseragamannya Tinggi sedangkan Indeks Dominansi rendah.

Pengaturan Sanksi Administratif Bagi Pelaku Usaha/Penyedia Pengadaan Barang/Jasa Pemerintah Budiansyah, Agus; Suryani, Danu; Erbiana, Nyi Mas Gianti B.
Karimah Tauhid Vol. 3 No. 8 (2024): Karimah Tauhid
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/karimahtauhid.v3i8.15004

Sanksi administratif adalah sanksi yang diberikan kepada pelanggar administrasi atau pelanggaran yang sudah ditentukan dalam perundang-undangan yang bersifat administratif. Dalam pengadaan barang/jasa sanksi administrasi merupakan pelanggaran yang selalu dilakukan pelaku usaha, oleh sebab itu perlu adanya pengaturan sanksi administrasi yang efektif agar dalam pelaksanaan pengadaan barang/jasa pelaku usaha dapat berkinerja lebih baik sehingga tujuan dari pengadaan barang/jasa dapat terpenuhi, Penelitian ini bertujuan untuk mengkaji tentang pengaturan sanksi administrasi bagi pelaku usaha/penyedia pengadaan barang/jasa pemerintah. Hasil penelitian ini menunjukan bahwa pengaturan sanksi administrasi dalam pengadaan barang/jasa terdapat dalam peraturan presiden nomor 12 tahun 2021 tentang perubahan atas peraturan presiden nomor 16 tahun 2018 tentang pengadaan barang dan jasa pemerintah dan di pertegas dengan peraturan LKPP Nomor 4 Tahun 2021 tentang pembinaan pelaku usaha pengadaan barang/jasa pemerintah yang merupakan pengganti dari peraturan lemabag kebijakan pengadaan barang dan jasa nomor 17 tahun 2018 tentang sanksi daftar hitam. Pelaku usaha yang melanggar pengaturan tersebut akan mendapatkan sanksi administrasi berupa pemutusan kontrak, pencairan jaminan, pembayaran denda dan sanksi daftar hitam.

Indeks Keanekaragaman Hasil Tangkapan Jala (Cast net) di Sungai Tabir Kelurahan Mampun Kecamatan Tabir Kabupaten Merangin Bibit Bibit; Lisna Lisna; Agus Budiansyah; Nelwida Nelwida; Eko Wijayanto; Fauzan Ramadhan
Jurnal Ilmu Perairan (Aquatic Science) Vol. 10 No. 3 (2022): November
Publisher : LPPM Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Penelitian ini bertujuan untuk mengetahui keanekaragaman ikan yang tertangkap pada Jala di Sungai Tabir meliputi jenis ikan, bobot ikan serta memberikan informasi mengenai kondisi perikanan di sungai Tabir saat ini. Penelitian ini dilaksanakan pada tanggal 15 mei 2022 sampai 19 juli 2022 di Sungai Tabir Kelurahan Mampun Kecamatan Tabir Kabupaten Merangin. Metode yang digunakan dalam penelitian ini yaitu metode survei. Analisis data yang digunakan adalah Komposisi Jenis, Indeks Keanekaragaman, Indeks Keseragaman dan Indeks Dominansi. Hasil tangkapan Jala selama 15 hari penelitian di Sungai Tabir yaitu Ikan Seluang Rasbora argyotaenia 234 ekor dengan rata-rata per hari 15,6 ekor, Ikan Senggiring Mystus Sp. 119 ekor dengan rata-rata 7,93 ekor, Ikan Kapiat Barbonymus schwanenfeldii 89 ekor dengan rata-rata 5,93 ekor, Ikan Nilem Osteochilus vittatus 77 ekor dengan rata-rata 5,13 ekor dan Ikan Beterung Pristolepis fasciata 55 ekor dengan rata-rata 3,67 ekor. Hasil indeks keanekaragaman 1,47, indeks keseragamannya 0,91 dan indeks dominansi 0,26. Dapat diambil kesimpulan bahwa Indeks Keanekaragaman Sedang, Indeks keseragamannya Tinggi, sedangkan Indeks Dominansi rendah.

Indeks Keanekaragaman Hasil Tangkapan Jala (Cast net) di Sungai Tabir Kelurahan Mampun Kecamatan Tabir Kabupaten Merangin Bibit, Bibit; Lisna, Lisna; Budiansyah, Agus; Nelwida, Nelwida; Wiyanto, Eko; Ramadhan, Fauzan
Ilmu Perairan (Aquatic Science) Vol. 10 No. 3 (2022): oktober
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Penelitian ini bertujuan untuk mengetahui keanekaragaman ikan yang tertangkap pada Jala di Sungai Tabir meliputi jenis ikan, bobot ikan serta memberikan informasi mengenai kondisi perikanan di sungai Tabir saat ini. Penelitian ini dilaksanakan pada tanggal 15 mei 2022 sampai 19 juli 2022 di Sungai Tabir Kelurahan Mampun Kecamatan Tabir Kabupaten Merangin. Metode yang digunakan dalam penelitian ini yaitu metode survei. Analisis data yang digunakan adalah Komposisi Jenis, Indeks Keanekaragaman, Indeks Keseragaman dan Indeks Dominansi. Hasil tangkapan Jala selama 15 hari penelitian di Sungai Tabir yaitu Ikan Seluang Rasbora argyotaenia 234 ekor dengan rata-rata per hari 15,6 ekor, Ikan Senggiring Mystus Sp. 119 ekor dengan rata-rata 7,93 ekor, Ikan Kapiat Barbonymus schwanenfeldii 89 ekor dengan rata-rata 5,93 ekor, Ikan Nilem Osteochilus vittatus 77 ekor dengan rata-rata 5,13 ekor dan Ikan Beterung Pristolepis fasciata 55 ekor dengan rata-rata 3,67 ekor. Hasil indeks keanekaragaman 1,47, indeks keseragamannya 0,91 dan indeks dominansi 0,26. Dapat diambil kesimpulan bahwa Indeks Keanekaragaman Sedang, Indeks keseragamannya Tinggi, sedangkan Indeks Dominansi rendah.

Performa Ayam Broiler yang Diberi Perlakuan Cairan Rumen Kerbau sebagai Sumber Enzim dalam Ransum Berbasis Jagung dan Bungkil Kedelai Budiansyah, Agus; Haroen, Ucop; Resmi, Resmi; Syafwan, Syafwan; Ramlah, Ramlah
Buletin Peternakan Tropis Vol. 4 No. 1 (2023)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.4.1.69-87

This study aimed to determine the effect of using buffalo rumen fluid as a source of enzymes (CRKSE) in corn and soybean meal-based rations on the performance of broiler chickens. A total of 240 day-old chicks (DOC) of the Ross strain were allocated into 24 groups, consisting of 6 treatments and 4 replications with 10 chicks each. The treatment was using CRKSE, namely R1: 0 ml, R2: 30.78 ml, R3: 61.56 ml, R4: 92.35 ml, R5: 123.12 ml, and R6: 153.90 ml per kilogram of ration, respectively equivalent to 0%, 0.6%, 1.2%, 1.8%, 2.4% and 3.0% extracted buffalo rumen fluid enzymes. The rations were incubated with CRKSE for 24 hours at room temperature. Chickens were kept for 5 weeks and provided food and drinking water ad libitum. The variables measured were feed consumption, body weight gain (BWG), feed conversion ratio (FCR), final body weight (FBW), carcass weight, and digestive organ weight. The study design was a completely randomized design, and data were analyzed using ANOVA. If there is a significant effect, proceed with Duncan's multiple range test (DMRT). The use of CRKSE in rations had a significant effect (P<0.05) on feed consumption and FCR, whereas on BWG and FBW of broiler chickens up to 5 weeks of age, carcass weight, and digestive organ weights had no significant effect (P>0.05). The DMRT test results proved that the treatment using CRKSE at the level of 30.78 ml/kg (R2) to the level of 153.9 ml/kg ration (R6) significantly (P<0.05) reduced feed consumption and FCR compared to R1, but not significantly different (P>0.05) between R2, R3, R4, R5, and R6. It can be concluded that the use of CRKSE from slaughterhouses in rations is effective in improving the performance of broiler chickens by reducing the FCR Key words: buffalo, broiler performance, enzyme, local feed, rumen fluid ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh penggunaan cairan rumen kerbau sebagai sumber enzim (CRKSE) dalam ransum berbasis jagung dan bungkil kedelai terhadap performa ayam broiler. Sebanyak 240 ekor anak ayam umur sehari (DOC) strain Ross dibagi menjadi 24 kelompok, terdiri atas 6 perlakuan dan 4 ulangan, setiap ulangan terdapat 10 ekor. Perlakuannya adalah penggunaan CRKSE yaitu R1 : 0 ml, R2: 30,78 ml, R3: 61,56 ml, R4: 92,35 ml, R5: 123,12 ml dan R6: 153,90 ml per kilogram ransum, masing masing setara dengan 0%, 0,6%, 1,2%, 1,8%, 2,4% dan 3,0% enzim cairan rumen kerbau hasil ekstraksi. Ransum diinkubasi dengan CRKSE selama 24 jam pada suhu ruang. Ayam dipelihara selama 5 minggu, pemberian makan dan air minum tersedia ad libitum. Peubah yang diukur adalah konsumsi ransum, pertambahan bobot badan (PBB), konversi ransum, Bobot badan akhir (BBA), bobot karkas dan bobot organ pencernaan. Rancangan penelitian adalah rancangan acak lengkap, dan data dianalisis dengan ANOVA. Bila terdapat pengaruh yang nyata, dilanjutkan dengan uji jarak berganda Duncan (DMRT). Penggunaan CRKSE dalam ransum berpengaruh nyata (P<0,05) terhadap konsumsi ransum dan konversi ransum, sedangkan terhadap PBB dan BBA ayam broiler sampai umur 5 minggu, bobot karkas dan bobot organ pencernaan tidak berpengaruh nyata (P>0,05). Hasil uji DMRT membuktikan bahwa perlakuan penggunaan CRKSE pada taraf 30,78 ml/kg (R2) sampai taraf 153,9 ml/kg ransum (R6) nyata (P<0,05) menurunkan konsumsi ransum dan angka konversi ransum dibandingkan R1, tetapi tidak berbeda nyata (P>0,05) antara R2, R3, R4, R5 dan R6. Dapat disimpulkan penggunaan CRKSE asal rumah potong hewan dalam ransum efektif memperbaiki performa ayam broiler dengan menurunkan angka konversi ransum. Kata kunci: cairan rumen, enzim, kerbau, pakan lokal, performa ayam broiler.

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