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Adaptation Of Sunflowers To Salinity Stress On Coastal Land Silvia Permata sari; Obel Obel; Elara Resigia; Winda Purnama Sari Purnama Sari; Jamsari Jamsari; Ali Rahmad; Seswita Seswita
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 4 No 1 (2022): Jurnal Agronomi Tanaman Tropika (JUATIKA)
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v4i1.1697

Abstract

Sunflower is one of the oil-producing plants that potentially can be developed as a food raw material, industrial, medicine, and cosmetics. Sunflower cultivation needs to be conducted in various lands in Indonesia; one of them is coastal regions. However, on one side, these lands were included on marginal land because the high level of salinity constrains it. This research aims to determine sunflower growth and thrive on salinity stress in the coastal land. This research applies a random group design with five treatments consisting of two accession and three sunflower varieties with three repetitions. The research result shows that the sunflower accession Ha1 growth and thrive on salinity stress in coastal land was quite significant compared to the accession Ha15 and Kanigara, Helina IPB, and BM1 IPB varieties. Generally, sunflower can adapt to stress salinity in coastal land.
IDENTIFIKASI HAMA KUTUDAUN (Hemiptera: Aphididae) PADA TANAMAN JAGUNG HIBRIDA (Zea mays L.) DI KABUPATEN SOLOK SUMATERA BARAT Silvia Permata Sari; Irfan Suliansyah; Novri Nelly; Hasmiandy Hamid
Jurnal Sains Agro Vol 5, No 2 (2020): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v5i2.466

Abstract

Jagung (Zea mays L.) merupakan tanaman serealia penting sebagai sumber karbohidrat bagi jutaan penduduk di dunia. Kutudaun (Hemiptera: Aphididae) merupakan salah satu hama penting pada tanaman jagung. Namun informasi tentang jenis kutudaun yang menyerang tanaman jagung hibrida di Solok, Sumatera Barat masih sangat terbatas. Tujuan penelitian ini adalah mengetahui jenis spesies hama kutudaun yang menyerang tanaman jagung di Nagari Bukit Payo, Kecamatan Tanah Garam, Kabupaten Solok. Penelitian ini dilaksanakan dari bulan Januari hingga Juni 2019. Metode yang digunakan dalam penelitian ini adalah survei. Tanaman jagung yang dijadikan sampel dipilih secara acak (sampling). Pengambilan kutudaun tersebut dilakukan sebanyak 2x dengan waktu pengambilan selama musim tanam jagung hibrida. Kutudaun diperoleh dengan cara mengumpulkan koloni kutudaun yang terdapat pada bagian daun, batang dan tongkol dari tanaman jagung. Kemudian kutudaun langsung dimasukkan ke dalam tabung eppendorf yang telah berisi alkohol 96% menggunakan kuas, lalu diberi label lokasi dan tanggal pengambilan sampel. Selanjutnya kutudaun sampel dibawa ke Laboratorium Bioekologi Serangga, Departemen Proteksi Tanaman, Fakultas Pertanian, Universitas Andalas untuk mengetahui jenis dan jumlah individu kutudaun. Kutudaun diidentifikasi berdasarkan karakter morfologinya dengan menggunakan buku panduan Aphids on the World’s Trees, Aphids on the World Crops, Aphids on the World’s Herbaceous Plants and Shrubs. Hasil penelitian menunjukkan bahwa tanaman jagung hibrida di Kecataman Tanah Garam, Kabupaten Solok terserang hama kutudaun (Hemiptera: Aphididae), namun populasi kutudaun tergolong rendah yaitu 262 kutudaun. Kutudaun yang ditemukan pada tanaman jagung di Nagari Bukit Payo, Kec. Tanah Garam, Kab. Solok adalah sebanyak 2 spesies yaitu Rhopalosiphum maidis Fitch dan Myzus persicae. Rendahnya populasi kutudaun yang menyerang tanaman jagung hibrida tersebut disebabkan karena beberapa faktor, seperti varietas jagung, pola tanam, dan praktek budidaya yang dilakukan oleh petani setempat.Kata kunci: Populasi, Aphididae, Jagung, Identifikasi, Kutudaun
Respon Hama Wereng Coklat Nilaparvata lugens Stal. (Hemiptera: Delphacidae) Terhadap Ketahanan Dan Kerentanan Varietas Padi Rein Estefanus Senewe; Silvia Permatasari; Marietje Pesireron
JURNAL BUDIDAYA PERTANIAN Vol 16 No 1 (2020): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2020.16.1.51

Abstract

Brown planthopper (BPH) is one of the important pest insects that attack rice plants, which indirectly becomes a vector for the spread of grassy dwarf and empty dwarf diseases caused by viruses, and can consume a lot of food in a short time so that it can cause damage explosions and great losses. The aim of this study was to compare the eating response of brown planthopper to resistant and vulnerable rice varieties through the measurement of secreted honeydews. The study was conducted at the Toxicology Laboratory, Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University. Rice seedlings for honeydew testing were 30 days after sowing. Testing was done by infesting 3 female BPH into a plastic cage with filter paper (Whatman No.40 9 cm in diameter) placed on the bottom, which had been sprayed with a solution of Ninhydrin 0.01 mg/ml in acetone. The treatment consisted of 3 rice varieties (Ciherang, IR-64 dan Pelita) on each ovened and non-ovened filter paper, with three replicates so that a total of 18 treatments were used by infesting each with the 3 female BPH nymphs. Whereas, 3 treatments with non-ovened papers, with 3 replicates, were infested with 3 female BPH imago each. So that the treatments involved a total of 27 feeding tubes. Honeydews released by BPH that ate on the test varieties for 24 hours were collected on filter papers and formed blue/purple spots. The extent of the honeydew spots formed was measured and analyzed. The results showed that the average value of phloem consumption in Ciherang, IR-64, and Pelita rice varieties showed that these three varieties were very susceptible to BPH sucking pests. The more food nutrients sucked by BPH pests from rice plants, the greater the number of honeydew spots and the wider the honeydew spots that were produced. The areas of honeydew spots of Pelita variety was 33.78 m2, IR-64 28.26 mm2, and Ciherang 22.44 mm2, respectively. Pelita rice varieties had a high susceptibility to BPH pests when compared to Ciherang and IR-64 because Pelita does not have resistance genes to BPH pests. Keywords: Ciherang, IR-64, Pelita, planthopper, rice, honeydew ABSTRAK Wereng batang coklat (WBC) merupakan salah satu serangga hama penting yang menyerang tanaman padi, yang secara tidak langsung menjadi vektor bagi penyebaran penyakit kerdil rumput dan kerdil hampa oleh virus, serta dapat mengkonsumsi makanan yang banyak dalam waktu singkat sehingga dapat menimbulkan ledakan kerusakan dan kerugian yang besar. Penelitian ini bertujuan untuk membandingkan respon makan WBC pada varietas padi tahan dan rentan melalui pengukuran embun madu yang disekresikan. Penelitian dilaksanakan di Laboratorium Toksikologi, Departemen Proteksi Tanaman Fakultas Pertanian, Institut Pertanian Bogor. Bibit padi untuk pengujian embun madu digunakan saat berumur 30 hari setelah semai. Pengujian dengan menginfestasikan WBC betina ke dalam kurungan plastik yang di bagian bawahnya diletakkan kertas saring (Whatman No. 40 berdiameter 9 cm) yang telah disemprot dengan larutan Ninhidrin 0,01 mg/mL aseton. Perlakuan yang terdiri dari 3 varietas padi (Ciherang, IR-64 dan Pelita) pada masing-masing kertas saring oven dan tidak oven dan dengan tiga ulangan sehingga total terdapat 18 perlakuan dengan menginfestasikan masing-masing 3 ekor nimfa betina WBC. Sedangkan pada 3 perlakuan kertas saring tanpa oven, dengan tiga ulangan, yang diinfestasikan masing-masing berupa 3 ekor imago betina WBC. Sehingga total perlakuan seluruhnya meliputi sebanyak 27 tabung makan. Embun madu yang dikeluarkan oleh WBC yang makan pada varietas uji selama 24 jam tertampung pada kertas saring dan membentuk bercak berwarna biru/ungu. Luas bercak embun madu yang terbentuk diukur dan dianalisis. Hasil penelitian menunjukkan bahwa nilai rata-rata tingkat konsumsi floem pada varietas padi Ciherang, IR-64 dan Pelita, menunjukkan bahwa ketiga varietas ini sangat rentan terhadap hama pengisap WBC. Semakin banyak nutrisi makanan yang disedot oleh hama WBC dari tanaman padi, maka semakin banyak jumlah spot bercak honeydew dan semakin luas spot bercak honeydew yang dihasilkan. Luas spot untuk varietas Pelita adalah 33,78 m2, IR-64 28,26 mm2, dan Ciherang 22,44 mm2. Varietas padi Pelita memiliki kerentanan yang tinggi terhadap hama WBC, bila dibandingkan dengan varietas Ciherang dan IR-64, karena varietas Pelita ini tidak memiliki gen ketahanan terhadap hama WBC. Kata kunci: Ciherang, honeydew IR-64, padi, Pelita, wereng
PENGEMBANGAN DAN PEMBERDAYAAN USAHA PERBENIHAN PADI BERSERTIFIKAT DI NAGARI SUNGAI BATANG, KECAMATAN TANJUNG RAYA, KABUPATEN AGAM Indra Dwipa; Irfan Suliansyah; Syafrimen Yasin; Dini Hervani; Silvia Permata Sari
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 11: April 2023
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v2i11.5393

Abstract

Kegiatan Pengabdian Kepada Masyarakat Membangun Nagari Tahun 2022 ini merupakan keberlanjutan kegiatan PKM Membantu Nagari Membangun Nagari tahun 2020 dan 2021, yang difokuskan kepada pengembangan dan pemberdayaan usaha perbenihan padi bersertifikat di Nagari Sungai Batang Kecamatan Tanjung Raya Kabupaten Agam. Lima kegiatan yang diberikan pada mitra PKM (KWT Semangat Berkarya) oleh Tim Pengabdian Unand, yaitu: 1). Sosialisasi teknologi pengeringan benih bersertifikat, 2). Penyuluhan dan sekolah lapang pengujian benih hasil penangkaran, 3). Sosialisasi penanganan hama gudang dan penyakit pasca panen, 4). Bimbingan teknis teknologi pengujian viabilitas benih berkualitas gabah hasil penangkaran, 5). Bimbingan teknis teknologi packing gabah hasil penangkaran. Lima kegiatan tersebut diberikan dalam bentuk sosialisasi dan sekolah lapang. Hasil pengabdian menunjukkan bahwa kegiatan PKM sudah terlaksana dengan baik, sukses, dan capaian tercapai 100%. Pada kegiatan pengabdian ini juga memperlihatkan adanya kolaborasi berbagai instansi, seperti BPSB Provinsi Sumatera Barat, POPT Kabupaten Agam, Instansi pemerintahan setempat. Mitra dari kegiatan PKM ini sangat berterima kasih karena dilatih menjadi penangkar benih padi bersertifikat, bahkan mendapatkan bantuan benih lainnya (kacang tanah) maupun alat produksi sebagai penangkar benih padi bersertifikat (mesin perontok gabah).
PENGARUH PEMBERIAN DOSIS DOLOMIT TERHADAP PERTUMBUHAN DAN HASIL TANAMAN JAGUNG (Zea mays. L) DI LAHAN GAMBUT Netti Herawati; Nasrez Akhir; Tasya Noor Aina; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 25, No 2 (2023): edisi April
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i2.2756

Abstract

This research is about the effect of dolomite dosing on the growth and yield of maize (Zea mays L.) on peat soil. This research aimed to obtain the best dose of dolomite on the growth and yield of maize (Zea mays L.) on peat soil. This research was conducted at Matobe Village, Hamlet of Sosoroat, South Sipora District, Mentawai Island. This study used the experimental method in a Randomized Block Design (RBD) with 3 levels of treatment (without using dolomite; 2 ton/ha dolomite; 4 ton/ha dolomite) so that 9 experimental units were obtained. The research data were analyzed statistically using F-test at 5% level. If F count is greater than F table 5%, then proceed with Duncan’s New Multiple Range Test (DNMRT) at the 5% real level. The result showed that the best dose of dolomite on the growth and yield of maize (Zea mays L.) on peat soil was 2 ton/ha dolomite which significantly affected plant height, cob diameter, and the amount of line in each cob.INTISARIPenelitian ini tentang pengaruh pemberian dosis dolomit terhadap pertumbuhan dan hasil tanaman jagung (Zea mays) di lahan gambut. Penelitian ini bertujuan untuk mendapatkan dosis terbaik dari kapur dolomit terhadap pertumbuhan dan hasil tanaman jagung di lahan gambut. Penelitian ini dilaksanakan di Desa Matobe Dusun Sosoroat, Kecamatan Sipora Selatan, Kabupaten Kepulauan Mentawai. Penelitian ini menggunakan metoda percobaan dalam Rancangan Acak Kelompok (RAK) dengan 3 taraf perlakuan (tanpa pemberian kapur dolomit; 2 ton/ha kapur dolomit; 4 ton/ha kapur dolomit) sehingga didapatkan 9 satuan percobaan. Data hasil penelitian dianalisis secara statistik dengan uji F pada taraf 5%. Jika F hitung lebih besar dari F tabel 5%, maka dilanjutkan dengan uji Duncan’s New Multiple Range Test (DNMRT) pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa dosis kapur dolomit terbaik untuk tanaman jagung (Zea mays L.) di lahan gambut adalah dosis 2 ton/ha yang berpengaruh nyata terhadap tinggi tanaman, diameter tongkol, dan jumlah baris per tongkol
Hubungan Pemahaman Tentang Bioteknologi Dengan Strategi Coping Stress Mahasiswa Tadris Biologi IAIN Kerinci Dalam Menerima Vaksin Covid-19 Silvia Permata Sari; Dharma Ferry; Emayulia Sastria
Symbiotic: Journal of Biological Education and Science Vol. 4 No. 1 (2023): Symbiotic: Journal of Biological Education and Science Vol. 4 No. 1 April 2023
Publisher : Institut Agama Islam Negeri Kerinci

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32939/symbiotic.v4i1.100

Abstract

The government's plan to vaccinate the Indonesian population for Covid-19 has encountered obstacles due to the emergence of hoaxes regarding the Covid-19 vaccine. Several students of Biology Department of IAIN Kerinci who have contracted biotechnology courses, that some of these students have not dared to be vaccinated against Covid-19 because they were affected by the Covid-19 vaccine hoax. The research was conducted on students who had contracted biotechnology courses at the Biology Department of IAIN Kerinci, using quantitative methods and correlational approaches. From the results of the study, it was found that the level of understanding of students' biotechnology was in a low category, namely 42.85% with a frequency of 27 respondents. Meanwhile, students' stress coping strategies in receiving the Covid-19 vaccine were in the high category, namely 84.12% with a frequency of 53 respondents. The results of the correlation analysis show a significance value of 0.007 and it means that there is a relationship between the two variables in this study, but the interpretation of the correlation is categorized as low with a correlation coefficient value of 0.335. This means that increasing understanding of biotechnology will affect the improvement of students' stress coping strategies in receiving the Covid-19 vaccine.
PENGARUH PEMBERIAN IBA DAN KOMPOSISI MEDIA TUMBUH TERHADAP PEMBENTUKAN AKAR DAN TUNAS SETEK TANAMAN MAWAR (Rosa sinensis) Netti Herawati; Benni Satria; Annisa Najla Salsabila Apri; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4694

Abstract

This research aims to determine the interaction between IBA concentration and growth media composition. Apart from that, determine the provision of IBA and the best composition of growing media for the formation of roots and shoots of rose cuttings. This research was carried out for 4 months, starting from March to June 2022 at the UPT Farm Experimental Garden, Faculty of Agriculture, Andalas University, Padang. The design used was a Factorial Design with 2 factors arranged in a Completely Randomized Design (CRD). The first factor is the provision of IBA which consists of 4 levels (0; 50; 100; 150 ppm). The second factor is the composition of the growing medium which consists of 3 levels: (soil + charcoal husk + cow manure; soil + coconut fiber powder (cocopeat); soil + sand + cow manure). The research data were analyzed statistically using the F test at the 5% level. If the calculated F is greater than the F table of 5%, then proceed with the Duncan's New Multiple Range Test (DNMRT) at level 5. The results of the research show that there is no interaction between the concentration of IBA (Indole-3 butyric acid) and the composition of the growing media in root formation and rose cuttings. The best IBA concentration for forming roots and shoots from rose cuttings is a concentration of 100 ppm, while the best growing medium for forming roots and shoots from rose cuttings is soil + sand + cow manure.
SELEKSI IN VITRO KALUS KEDELAI (Glycine max L.) HASIL INDUKSI MUTASI MENGGUNAKAN ETIL METAN SULFONAT TOLERAN KEKERINGAN DAN ALUMINIUM Gustian Gustian; Muhammad Rafli Ibrahim; Benni Satria; Ryan Budi Setiawan; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4695

Abstract

Soybean (Glycine max L.) is a type of nutritious food crop because soybean seeds have high protein amino acid content. Increasing domestic production for soybean self-sufficiency needs to be done by expanding the planting area. The objective of this research is to examine the effect of ethyl methane sulfonate concentration and obtain soybean plants tolerant to drought and aluminum stress through in vitro selection. This research was conducted from November 2023 to January 2024 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University. The research was arranged in a Completely Randomized Design consisting of 12 treatment levels of the combination of concentrations of abiotic selection agents PEG 4000 and AlCl3 induced by EMS mutation and 12 treatment levels of the combination of concentrations of abiotic selection agents PEG 4000 and AlCl3 not induced by EMS mutation with 3 replications. Data analysis was performed using independent sample t-test at a significance level of 5% and interpreted descriptively. The results showed that mutation induction using ethyl methane sulfonate (EMS) did not produce soybean somatic embryo mutants tolerant to drought and aluminum stress. Meanwhile, results from non-mutated callus produced somatic embryos suspected to be potential putative somaclonal candidates tolerant to drought and aluminum after double in vitro selection using 10% PEG 4000 and 150 ppm AlCl3 selection agents.
INVENTARISASI SPESIES AND INTENSITAS SERANGAN SERANGGA VEKTOR TANAMAN TERUNG (SOLANUM MELONGENA L.) DI SUMATERA BARAT Lailatun Najmi; Silvia Permata Sari; Yaherwandi Yaherwandi
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 8, No 4 (2023): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v8i4.14073

Abstract

Tanaman terung saat ini menjadi tanaman yang menggiurkan dalam sektor pertanian di tengah-tengah masyarakat di Sumatera Barat. Ditengah meningkatnya produksi tanaman terung juga terdapat laporan hama dan penyakit tanaman yang semakin marak. Beberapa hama yang menyerang tanaman terung ada yang berperan sebagai vektor penyebab dan penyebar penyakit tanaman. Oleh karena itu, penelitian ini bertujuan untuk mengetahui jenis dan populasi serangga vektor, gejala serangan serangga vector, dan intensitas serangan serangga vector pada tanaman terung di Sumatera Barat. Penetapan sampel diambil secara diagonal dengan lima titik pengamatan. Masing-masing titik diambil lima sampel tanaman secara acak. Pengamatan dilakukan sebanyak tiga kali, dilakukan pada empat lokasi yang mewakili sentra daerah produksi terung di Sumatera Barat, yaitu di Kecamatan Kuranji, Kecamatan Pauh, Kecamatan Batang Anai, dan Kecamatan Sitoga. Serangga vector yang ditemukan pada tanaman terung ditemukan diantaranya Amrasca devastans, Bemisia tabaci, Aphids gosypii, Thrips sp, dan Paracoccus sp serta juga ditemukan Acanthocepala terminalis dan Nezara viridula meskipun dalam jumlah yang sedikit  Populasi serangga vector dominan yang ditemukan pada tanaman terung dengan intensitas serangan juga mencapai 100% yaitu Bemisia tabaci dan Amrasca devastans. Peningkatan intensitas serangan serangga vector seiring dengan meningkatnya populasi serangga vector.
Pertumbuhan dan Hasil Kentang G1 Melalui Modifikasi Media Tanam dan Aplikasi Zat Pengatur Tumbuh Suliansyah, Irfan; Hervani, Dini; Sari, Silvia Permata; Muhsanati, Muhsanati; Ekawati, Fitri Ekawati,
Jurnal Agroekoteknologi Vol 15, No 2 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v15i2.23183

Abstract

Success in cultivating potatoes is the use of good quality potato seeds. Good quality potato seeds are produced through several stages starting from in vitro culture to extension seed production. One of the important stages in potato seed production is the production of first generation (G1) potato seeds. Efforts to increase the yield of G1 tubers can be made through modifying the planting media and applying growth regulators. This research aims to produce G1 potato tubers by adjusting the composition of the planting media (cocopeat, cocofiber, charcoal husk) and applying a growth inhibitor (Daminozide). The research consisted of four series of experiments. Each experiment was carried out using a Completely Randomized Design (CRD) with six treatments, each repeated four times. Experiment 1 (a. Cocopeat 100% + 0% husk charcoal; b. Cocopeat 20% + 80% husk charcoal; c. Cocopeat 40% + 60% husk charcoal; d. Cocopeat 60% + 40% husk charcoal; e. Cocopeat 80% + Charcoal Husk 20%; f. Cocopeat 0% + Charcoal Husk 100%. Experiment 2 (a. Cocofiber 100%; b. Cocopeat 100%; c. Cocopeat 20% + Cocofiber 80%; d. Cocopeat 40% + Cocofiber 60%; e. Cocopeat 60% + Cocofiber 40%; f. Cocopeat 80% + Cocofiber 20%. Experiment 3 (a. Cocopeat + Cocofiber + husk charcoal (1 : 1 : 1); b. Cocopeat + Cocofiber + charcoal husk (1 : 1 : 2); c. Cocopeat + Cocofiber + husk charcoal (1 : 2 : 1); d. Cocopeat + Cocofiber + husk charcoal (2 : 1 : 1). Experiment 4 (a. Daminozide 0 ppm; b. Daminozide 500 ppm; c. Daminozide 1000 ppm; d. Daminozide 1500 ppm). From the research results it can be concluded: 1) The combination treatment of 80% cocopeat + 20% cocofiber is the best treatment combination in the vegetative growth phase; 2) Composition treatment 60% cocopeat + 40% husk charcoal is the best treatment for the growth and number of G1 potato tubers; 3) Treatment of the composition of the planting media with cocopeat, cocofiber, and husk charcoal with a composition of 1:1:1 produces the highest number of tubers; 4) The best concentration of daminozide for the growth and production of G1 potato seed tubers is 3500 ppm.