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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Microbiology Indonesia Jurnal Penelitian Hasil Hutan
Zainal Alim Mas'ud
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Microbiology Indonesia

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Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. ROFIQ SUNARYANTO; BAMBANG MARWOTO; TUN TEDJA IRAWADI; ZAINAL ALIM MAS'UD; LIESBETINI HARTOTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6996.369 KB) | DOI: 10.5454/mi.4.2.7

Abstract

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out. Marine sediment samples were obtained from six different places at Banten West Coast. Isolation was conducted using two pretreatment methods, acid and heat shock pre-treatment. A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134. Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp. Production of active compound from A11 isolate used yeast peptone medium. Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC. A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Co-Authors . Sugihartati . Taufiq Ahmad Syahreza Akhmad Endang Zainal Hasan Aniversari Apriana BAMBANG MARWOTO Bambang Padmadi Bram Kusbiantoro Charlena Dewi Praptiwi Dimas Adrianto Djarot Sasongko Hami Seno Djumali Mangunwidjaja Helmy Ramadhan Al Anshary Iswandi Anas Kapelle, Imanuel B. D. Liesbetini Hartoto Meika Syahbana Rusli Muhammad Taufan Fatahajudin Niken Dyah Wanodyanti Rofiq Sunaryanto Satya Nugroho Tri Joko Santoso Tun Tedja Irawadi