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All Journal Jurnal Ilmu Pertanian Indonesia AL-HAYAT: Journal of Biology and Applied Biology Floribunda
Asri Febriana
Universitas Islam Negeri Walisongo Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Kejadian Indel Simultan pada Intron 7 Gen Branched-Chain α-Ketoacid Dehydrogenase E1a (BCKDHA) pada Sapi Madura Asri Febriana; Achmad Farajallah; Dyah Perwitasari
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 2 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1157.4 KB) | DOI: 10.18343/jipi.20.2.97

Abstract

Madura cattle is one of the Indonesian local cattle breeds derived from crossing between Zebu cattle (Bos indicus) and banteng (Bos javanicus). Branched-chain α-ketoacid dehydrogenase (BCKDH) is one of the main enzyme complexes in the inner mitochondrial membrane that metabolizes branched chain amino acid (BCAA), ie valine, leucine, and isoleucine. The diversity of the nucleotide sequences of the genes largely determine the efficiency of enzyme encoded. This paper aimed to determine the nucleotide variation contained in section  intron 7, exon 8, and intron 8 genes BCKDHA on Madura cattle. This study was conducted on three Madura cattle that used as bull race (karapan), beauty contest (sonok), and beef cattle. The analysis showed that the variation in intron higher than occurred in the exon. Simultaneous indel found at base position 34 and 68 in sonok cattle. In addition, the C266T variant found in beef cattle. These variants do not cause significant changes in amino acids. There was no specific mutation in intron 7, exon 8, and intron 8 were found in Madura cattle designation. This indicated the absence of differentiation Madura cattle designation of selection pressure of BCKDHA gene.
Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Asri Febriana; Achmad Farajallah; Baiq Farhatul Wahidah; Dyah Perwitasari
Al-Hayat: Journal of Biology and Applied Biology Vol 5, No 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
AUTHENTICATION OF THREE WAX APPLES CULTIVARS (SYZYGIUM SAMARANGENSE (BLUME) MERR. & L.M. PERRY) BASED ON MORPHOLOGICAL CHARACTER AND FRUIT METABOLITE PROFILE Annisa Nur Rachmah; Asri Febriana; Niken Kusumarini; Eka Oktaviani; Arnia Sari Mukaromah
Floribunda Vol. 7 No. 2 (2023): Floribunda April 2023
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v7i2.2023.409

Abstract

Wax apple is one of the superior fruits of Demak, especially cultivars 'Citra', 'Delima' and ‘Madu Deli Hijau'. Research on the wax apple of the three cultivars is still limited. Therefore, research on the authentication of wax apple cultivars based on morphological characters and metabolite profiles present in one location is important to do. The aims of this study were to identify morphological characters and analyze metabolite profiles, to analyze relationship and to identify morphological characters and metabolite marker profiles in the authentication activities of three wax apples cultivars. Sampling was in Boyolali Village, Demak Regency. The stages of this research included morphological characterization of stem, leaf, fruit and seed organs, phylogeny analysis with MVSP, metabolite profile analysis using GC-MS, and determination of morphological characters and metabolite profiles with PCA. The results showed that there were morphological diversity and metabolite profile variations of the three cultivars. Relationship analysis based on morphological characters and metabolite profiles resulted in different grouping patterns. 'Madu Deli Hijau' and 'Citra' are closely related based on morphological characters and a combination of morphological characters and metabolite profiles. Morphological characters in the authentication of the 'Citra' (dark red exocarp color), 'Delima' (bright red exocarp color, smooth and flat fruit surface, jagged fruit shape), in 'Madu Deli Hijau' (elongated leaf shape and exocarp is green with a pink tinge). Authentication of probable marker metabolite profiles on 'Citra', 'Delima' and 'Madu Deli Hijau' wax apples of 10, 7 and 5 compounds.
Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Arnia Sari Mukaromah; Malia Ulfah; Annisa Nur Rachmah; Muhammad Ramdhani Arfan; Niken Kusumarini; Asri Febriana
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Abstract

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet.  The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.
Co-Authors ACHMAD FARAJALLAH Annisa Nur Rachmah Annisa Nur Rachmah Arnia Sari Mukaromah Arnia Sari Mukaromah Baiq Farhatul Wahidah DYAH PERWITASARI -FARAJALLAH Eka Oktaviani Malia Ulfah Muhammad Ramdhani Arfan Niken Kusumarini Niken Kusumarini