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KAJIAN KARAKTER MORFOLOGI MIKROSPORA TEMBAKAU VIRGINIA YANG MENGALAMI CEKAMAN PELAPARAN DAN SUHU TINGGI SECARA IN VITRO -, Baiq Farhatul Wahidah
Teknosains Vol 8, No 2 (2014): JULI
Publisher : Fakultas Sains dan Teknologi UIN ALauddin

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Abstract

The effect of treatment combination by heat shock (34oC) and starvation medium on embryogenesis induction in microspore culture of selected genotype of Nicotiana tabacum L.cv. Virginia was examined.The microspores which were isolated aseptically by maceration and centrifugation, were cultured on starvation medium (B medium) without sugar and nitrogen during 6 days at 34oC. The culture contained relatively homogeneous population of developed stage microspore (late uninucleate stage). The viability and development of microspore was obsevered. DAPI (4,6-diamidino-2-phenylindole) was used to stain the nucleus. Stained microspores were examined under a fluorescence microscope.The result showed that during stress treatment by starvation and heat shock (34oC) the structure of microspore change into 3 types of embryogenic microspore. Type 1 was identical to the late uninucleate microspore stage; in type 2 the vacuole of microspore was fragmented and peripheral cytoplamic pocket contain the nucleus; and type 3 the nucleus was positioned in cytoplamic pocket in the centre of the microspore.Key words: Nicotiana tabacum L.cv. Virginia, Starvation, heat shock.
Perbandingan Kecepatan Fotosintesis Pada Tanaman Sawi Hijau (Brassica juncea) yang diberi Pupuk Organik dan Anorganik Hasbiah, St; Farhatul Wahidah, Baiq
Biogenesis Vol 1, No 1 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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This study was conducted to determine the comparison of photosynthetic rate between organic and inorganic plant with mustard greens (Brassica juncea) as sample. The experimental design was a randomized block design with 3 times respectively using compost for organic crops and NPK for inorganic crops. Doses used is 20 g / kg soil for both. The results showed an average increase of oxygen pressure in the light reactions of organic crops is 0.123 hPa/s whereas the inorganic plant is0.028 hPa / s. The average pressure of oxygen organic crops decreased to 0.104 hPa / s whereas the inorganic plant -0.037 hPa / s in the dark reaction. Analysis of the data in this study using the unpaired t test and the results showed a significant difference between the rate of photosynthesis in plants organic and inorganic.Keywords: dark reaction, inorganic plant, light reaction, organic plant, the rate of photosynthesis
Preparasi Kromosom Fase Mitosis Markisa Ungu (Passiflora edulis) Varietas Edulis Sulawesi Selatan Muhlisyah, Nurul; Muthiadin, Cut; Farhatul Wahidah, Baiq; Rasdianah Aziz, Isna
Biogenesis Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Passion fruit (Passifloraceae) was derived from tropical South America. Kind of passion fruit that widely cultivated in South Sulawesi is purple passion fruit (Passiflora edulis). This study aimed to observe the activity of Passiflora edulis chromosome at different stages of cell division and observed active mitosis time. The study was conducted by using exploratory descriptive study and obtained results a mitotic on Passiflora edulis occurs at 09.00 until 15.00 pm. Prophase seen in 5 minute after observation, prometaphase in 80 minute after the first observation, metaphase in 154 minutes after the first observation, anaphase in 227 minutes after the first observation, and telophase in 317 minutes after the first observation.Keywords: Chromosome, Mitosis, Passiflora edulis, Purple Passion Fruit
Pengaruh Penambahan Air Kelapa Terhadap Pertumbuhan Stek Mikro Tanaman Krisan (Chrysanthemum indicum) Secara In Vitro M, Mustakim; Farhatul Wahidah, Baiq; Al-Fauzy, Adi
Prosiding Seminar Biologi 2015: Seminar Nasional Mikrobiologi Kesehatan dan Lingkungan
Publisher : Prosiding Seminar Biologi

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Langkah antisipatif pemenuhan kebutuhan massal benih krisan bermutu yang dilakukan dengan perbanyakan secara in vitro. Penelitian bertujuan untuk mengetahui pengaruh penambahan air kelapa terhadap stek mikro tanaman Krisan yang ditanam secara in vitro, serta perlakuan mana yang memberikan pengaruh terbaik. Penelitian telah dilaksanakan di laboratorium Botani Fakultas Sains dan Teknologi UIN Alauddin Makassar. Penelitian ini berupa penelitian eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL) satu faktor yaitu penambahan air kelapa yang terdiri atas empat perlakuan yakni 0 ml/l, 50 ml/l, 100 ml/l, dan 150 ml/l. parameter yang diamati adalah pembentukan jumlah akar, jumlah daun, tinggi planlet, dan berat planlet. Data yang diperoleh setelah dianalisa secara statistik inferensial yaitu uji F (varians) pada taraf kepercayaan α 0,05. Hasil penelitian menunjukkan bahwa penambahan air kelapa dengan konsentrasi 150 ml/ memberikan hasil terbaik terhadap pembentukan jumlah daun, jumlah akar, tinggi planlet dan berat planlet.Kata Kunci: Chrysanthemum indicum, Murashige dan Skoog, stek dan air kelapa
Identifikasi Jenis-Jenis Poaceae di Desa Samata Kabupaten Gowa Sulawesi Selatan Bohari, Megawati; Farhatul Wahidah, Baiq
Prosiding Seminar Biologi 2015: Seminar Nasional Mikrobiologi Kesehatan dan Lingkungan
Publisher : Prosiding Seminar Biologi

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Penelitian ini merupakan penelitian deskriptif yang dilakukan untuk mengetahui jenis-jenis Poaceae di Di Desa Samata Kabupaten Gowa Sulawesi Selatan. Metode yang dilakukan adalah plot dengan membuat beberapa plot di daerah penelitian. Daerah peneitiannya sendiri dibagi menjadi 20 stasiun yang ditentukan secara purposive sampling yaitu area yang memiliki kepadatan populasi tumbuhan yang cukup tinggi. Selanjutnya Poaceae yang di jumpai diidentifikasi berdasarkan ciri morfologinya yaitu akar, batang, daun, bunga, buah, dan biji. Hasil identifikasi menunjukkan bahwa di lokasi penelitian terdapat 18 jenis Poaceae yaitu Axonopus compressus, Bambusa apus, Bambusa vulgaris, Chloris barbata, Cymbopogon nardus Cynodon dactylon, Dactyloctenium aegyptium, Digitaria sanguinalis, Eleusin indica, Eragrotis amabilis, Eulalia amaura, Imperata cylindrical, Paspalum commersonii, Pennisetum purpureum, Pogonatherum paniceum, Sorghum halapenses, Oryza sativa dan Zea mays.Kata Kunci: Desa Samata, identifikasi, Poaceae
Perbedaan Pengaruh Media Tanam Serbuk Gergaji dan Jerami Padi Terhadap Pertumbuhan Jamur Tiram Putih (Pleurotus ostreatus) Farhatul Wahidah, Baiq; Adi Saputra, Firman
Biogenesis Vol 3, No 1 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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This research is an experimental research that aims to identify differences effect of growing media sawdust and rice straw on mycelium development time, the number of fruiting bodies and wet weight of Pleurotus ostreatus grown in the media (baglog). This research conducted in the Botanical Laboratory, Biology department, UIN Alauddin Makassar. The method in this research is completely randomized design with two types of growing media treatment. Each treatment consists of five repetitions obtained 10 baglog in observation. The data obtained were analyzed using inferential statistics t test, confidence level 0.05 through software SPSS 16.0. The results showed that there was a significant differences between the growth of Pleurotus ostreatus grown on rice straw media and sawdust media.Keywords: Pleurotus ostreatus, sawdust and rice straw media, the mycelium growth, the number of fruiting bodies
Karakterisasi Morfologi Anggrek (Orchidaceae) di Hutan Kecamatan Ngaliyan Semarang Shidiqy, Hasby Ash; Wahidah, Baiq Farhatul; Hayati, Nur
AL-HAYAT: Journal of Biology and Applied Biology Vol 1, No 2 (2018): Vol. 1, No. 2, Tahun 2018
Publisher : AL-HAYAT: Journal of Biology and Applied Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v1i2.3761

Abstract

Orchid is one ofthe flowering plants with a very high level of diversity, including the diversity of morphological characters it has. This study aims to determine the diversity of orchids (orchidaceae) seen morphologically in the Ngalian sub-district forest in Semarang city. The method used in this study is cruise method and characterization techniques. Analysis of research data was carried out descriptively and displayed in the form of tables and photos. Orchids in the teak forest cover 940 ha, spread in 4 villages, namely Wonosari village, Gondoriyo village, Podorejo village and Wates village. The results showed that there were 3 types of orchid plants (orchidaceae). The types of orchids include Rhynchostylis retusa (L.) Bi., Rhynchostylis gigantea (Lindl.) Rindl. and Dendrobium crumenatum (L.) Sw. The diversity of morphological characters in each species of orchid such as diversity in leaf color, leafshape, leaftip shape, stem color, root type etc.
Inventarisasi Jenis-jenis Rumput di Jalur Pendakian Gunung Ungaran Nuhaa, Muhammad Hasiibun; Lianah, Lianah; Wahidah, Baiq Farhatul
Al-Hayat: Journal of Biology and Applied Biology Vol 2, No 2 (2019)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v2i2.4663

Abstract

Mount Ungaran has very varied vegetation which includes grass plants. The purpose of this study was to find out the types of Poaceae family grass found on the Mount Ungaran hiking path. This type of research is a qualitative research field. The object of the research is the types of grass belonging to the family Poaceae. Data collection techniques used in this study were observation and characterization. The results of the study obtained 29 species from 23 genera.
Inventarisasi Tumbuhan Obat di Jalur Pendakian Gunung Ungaran Khoirurrais, Muhamad; Ismail, Ismail; Wahidah, Baiq Farhatul
Al-Hayat: Journal of Biology and Applied Biology Vol 2, No 1 (2019)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v2i1.4648

Abstract

Mount Ungaran has a high varied vegetation which includes medicinal plants. The purpose of this study is to know the types of medicinal plants found in the hiking trails of Mount Ungaran. The method used in this research is the cruising method. The object of research was spermatophyta medicinal plants. Data collection techniques used in this study are observation, characterization and document review. The results of the study obtained 43 types of medicinal plants belonging to 30 families with the most families were Asteraceae family with the number of 5 species. But unfortunately these plants have not been maximally utilized by the surrounding community due to lack of community knowledge about the potential of these plants and their use especially as a medicinal plants.
Etnobotani Tanaman Kelor (Moringa oleifera Lam.) di Desa Kedungbulus Gembong Pati Dhea Dani, Brintan Yonaka; Wahidah, Baiq Farhatul; Syaifudin, Andang
Al-Hayat: Journal of Biology and Applied Biology Vol 2, No 2 (2019)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v2i2.4659

Abstract

The potential of  Moringa tree related to health properties is still not fully utilized by the people in Pati. Mrs. Muryati, a resident of Kedungbulus Village, Gembong Subdistrict, Pati, was one of the residents who was moved to campaign for the use of Moringa leaves. The purpose of this study was to determine public perceptions about the potential of Moringa plants in the village of Kedungbulus Gembong Pati. This research uses survey methods which include: literature study, field observations, interviews using questionnaires, semistructure interview techniques and using random sampling techniques and purposive sampling. Random sampling sampling from the community taken randomly, while purposive sampling sampling from community leaders such as informants of production houses, village heads, shamans etc. The results obtained from the relationship between community and Moringa plants are explained from interviews with the public perception of  Moringa plants.
Co-Authors Abadiyah, Auliya Saadatul ACHMAD FARAJALLAH Adi Al-Fauzy Adi Al-Fauzy, Adi Adi Sutanto Aidatul, Agustina Alfina, Lina Andang Syaifudin Anif Rizqianti Hariz Anif Rizqianti Hariz Aprilianingsih, Risqi Apriliyani, Riski Arifin Surya Dwipa Irsyam Asma Lutfi, Asma Asri Febriana Asri Febriana, Asri Atika Wijaya Auliya Saadatul Abadiyah Azizah, Ismatul Birran, Regan Abadi Khalid Putra Yusac Brintan Yonaka Dhea Dani Cut Muthiadin Devi Octavia Dhea Dani, Brintan Yonaka Dinalhaq, Putri Cahya Dinalhaq, Putri Cahya Dyah Perwitasari DYAH PERWITASARI -FARAJALLAH Eri Sulis Hardianto Erna Afdal Fadly Husain FATIMA, SITI Fatmawati Nur Firman Adi Saputra Firman Adi Saputra, Firman Harto Wicaksono, Harto Hasby Ash Shidiqy Hasrul Hasrul Hasyimuddin Hasyimuddin I Putu Gede P. Damayanto I Putu Gede P. Damayanto Irsyad Kamal Irsyam, Arifin Surya Dwipa Ismail Ismail Ismail Ismail Isna Rasdianah Aziz Isna Rasdianah Aziz Isna Rasdianah Aziz Khasanah, Rizkiati Khoirurrais, Muhamad Kuncoro Bayu Prasetyo Lailatul Qodriyah Lala Nita Sari Lianah Lianah, Lianah Masriany, Masriany Megawati Bohari Megawati Bohari, Megawati Miswari Miswari Miswari Miswari, Miswari Muhamad Khoirurrais Muhammad Hasiibun Nuhaa Muhammad Rifqi Hariri Muhammad Rifqi Hariri Mulyanah, Wiwin Murhadi Murhadi Mustakim M, Mustakim Mustakim Mustakim Nawang, Mugi Niken Kusumarini Niken Kusumarini, Niken Nisa', Galih Kholifatun Nuhaa, Muhammad Hasiibun Nur Hayati Nur Hayati Nur Hayati Nurkhalis A Gaffar Nurlaila Sari Nurlailah Mappanganro Nurman Nurman Nurul Muhlisyah Nurul Muhlisyah Rahma Ziyan Firdausia Rahmat Fajrin Alir Rini Iswari Risqi Aprilianingsih Rizkiati Khasanah Rusmadi Rukmana Rusmadi Rusmadi Rusmadi Rusmadi Saifullah Hidayat Sary, Dian Puspita Shidiqy, Hasby Ash Sri Mulyani Sri Mulyani St Hasbiah St Hasbiah Syaifudin, Andang Ulya, Fadhliyatul Zahro, Shofiyyatuz Zul Janwar Zulkarnain Zulkarnain