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All Journal HAYATI Journal of Biosciences Vegetalika Jurnal Ilmu Pertanian Jurnal Ilmu Lingkungan Gontor AGROTECH Science Journal Jurnal Agro Planta Tropika Panrita Abdi - Jurnal Pengabdian pada Masyarakat Kultivasi Agriprima : Journal of Applied Agricultural Sciences Caraka Tani: Journal of Sustainable Agriculture Floribunda Penbios: Jurnal Pendidikan Biologi dan Sains J-PEN Borneo : Jurnal Ilmu Pertanian JURNAL AGROSAINS : Karya Kreatif dan Inovatif ABDIPRAJA (Jurnal Pengabdian kepada Masyarakat) Proceedings Series on Physical & Formal Sciences PESARE: Science and Engineering Service Journal Biosaintifika: Journal of Biology & Biology Education
Eka Oktaviani
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Jenderal Soedirman
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Mechanical Engineering Public Health Social Sciences Other

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Amylose profile and rice grain morphology of selected F6 lines derived from a crossing of Black Rice and Mentik Wangi for the development of waxy pigmented rice Eka Oktaviani; Suprayogi Suprayogi; Zulfa Ulinnuha
Jurnal Ilmu Pertanian Vol 6, No 2 (2021): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.61867

Abstract

Research on rice plant breeding to get superior black rice varieties with tender rice texture can be carried out by crossing Black Rice and Mentik Wangi variety. The rice lines derived from a crossing of these two varieties have recently reached the F6 line. The texture of rice is distinguished by the amylose content. The lower of the rice amylose, the more tender of the rice texture, and vice versa. This study aimed to find out the profile of amylose content and the grains morphology of the lines that will be developed as waxy pigmented rice. Analysis of amylose content was carried out using iodine-colorimetry methods. The quantification of amylose was measured based on the regression of the standard amylose curve. The results showed that all the F6 lines had the potential to be developed as waxy pigmented rice. Based on the Kruskall Wallis test, there was a variance in the amylose profile average of the eight genotypes. In terms of grains morphology, the line 482-17-7 and 482-17-18 had a combination colour between the two parents. In addition, there was a significant difference in grains size between the genotypes and the grain size of all lines were classified in the medium size. F6 lines resulted from the crossing between Black Rice and Mentik Wangi are recommended to be used as superior varieties of waxy pigmented rice.
Karakter Rhizobakteri Pelarut Fosfat Potensial dari Rhizosfer Tumbuhan Mangrove Teluk Awur Kabupaten Jepara secara Mikrobiologi Eka Oktaviani; Arina Tri Lunggani; Rejeki Siti Ferniah
Jurnal Ilmu Lingkungan Vol 18, No 1 (2020): April 2020
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.13 KB) | DOI: 10.14710/jil.18.1.58-66

Abstract

Ekosistem mangrove Teluk Awur yang terletak di Kabupaten Jepara, Provinsi Jawa Tengah, merupakan  salah satu ekosistem mangrove yang mengalami kerusakan cukup parah karena perluasan lahan budidaya ikan (tambak), sehingga mendorong terjadinya erosi pantai. Peremajaan kembali dan konservasi ekosistem mangrove dapat dilakukan dengan menggunakan bakteri tanah yang mampu mendukung pertumbuhan tanaman atau yang biasa disebut Plant Growth Promoting Rhizobacteria (PGPR). Salah satu mekanisme pendukung pertumbuhan tanaman oleh kelompok PGPR adalah dengan aktivitas pelarutan fosfat karena fosfat dalam tanah berada dalam bentuk yang sulit diserap oleh tanaman. Penelitian ini bertujuan untuk memperoleh isolat Rhizobakteri pelarut fosfat yang unggul dalam melarutkan fosfat secara in-vitro dan mengetahui karakter isolat yang diperoleh. Isolasi dan penapisan rhizobakteri pelarut fosfat dilakukan menggunakan medium Pikovskaya agar. Karakterisasi isolat potensial dilakukan secara mikrobiologi dan atau uji aktivitas biokimia. Hasil penelitian menunjukkan bahwa rhizobakteri pelarut fosfat potensial yang berhasil diisolasi, secara mikrobiologi teridentifikasi ke dalam genus Enterobacter.
OPTIMALISASI PENGOLAHAN SAMPAH RUMAH TANGGA MENJADI PUPUK ORGANIK DAN BUDIDAYA SAYURAN DI PP AL-JAMIL, PURWOKERTO Ni Wayan Anik Leana; Prasmadji Sulistyanto; Eka Oktaviani; Zulfa Ulinnuha
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 6 No. 1 (2022): Jurnal Panrita Abdi - Januari 2022
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v6i1.12388

Abstract

Abstract. PP Al Jamil is an Islamic boarding school in  Banyumas regency, Central Java, with 126 pupils. This number of occupants would certainly produce a significant amount of organic household waste. The technology transfer introduced to the school as a community service was a method to turn organic waste into organic fertilizers for growing vegetables. So far, vegetables consumed in PP Al Jamil have been obtained from the nearest markets. A pre-trial survey conducted on the pupils in March 2020 showed that the majority (90%) understood the difference between organic and inorganic wastes. Furthermore, some students had sorted wastes into those two categories (61.22%), generated organic fertilizers from household waste (53.06%), and grown vegetables (65.30%). Based on this information, the technology transferred to the pupils increased the knowledge and skills on producing organic fertilizers and cultivating vegetables. The procedures comprised lectures, discussions, practices, and counseling on the above subjects, including pest control, fertilizing schedule, and plant care. A post-trial survey conducted in September 2020 indicated that the pupils' understanding of waste types increased by 10%, while their experience in vegetable farming increased by 26.37%. Furthermore, this community service activity reduced the school's daily expenditure on vegetables by 50%. --- Abstrak. PP Al Jamil salah satu pondok pesantren di kabupaten Banyumas, Jawa Tengah memiliki santri sejumlah 126 orang. Jumlah ini tentu menghasilkan sampah rumah tangga yang tidak sedikit. Alih teknologi yang diperkenalkan dalam pengabdian kepada masyarakat ini adalah teknik pengolahan sampah organik adalah dengan menjadikan pupuk organik dan memanfaatkannya untuk budidaya sayuran. Tujuan pengabdian kepada masyarakat ini adalah menyampaikan informasi mengenai teknik pengolahan sampah organik dan memanfaatkannya untuk budidaya sayuran. Selama ini semua sayur yang dikonsumsi di PP Al Jamil diperoleh dengan membeli di pasar terdekat. Hasil survey terhadap santri di PP Al Jamil pada bulan Maret 2020 menunjukkan bahwa mayoritas (90%) santri mengetahui macam-macam sampah yaitu organik dan anorganik. Berdasarkan pengalaman santri, 61,22% menyatakan pernah melakukan pemilahan sampah antara organik dan anorganik, membuat pupuk dari sampah yang dihasilkan (53,06%), serta budidaya sayuran (65,30%). Berdasarkan data ini pemecahan masalah yang ditempuh adalah dengan peningkatan pengetahuan dan keterampilan santi dalam membuat pupuk organik dan budidaya sayuran. Metode yang digunakan ceramah, diskusi, praktek langsung dan pendampingan pembuatan pupuk organik, budidaya sayuran termasuk pengendalian hama penyakit, pemupukan dan perawatan tanaman. Survey pada September 2020 menunjukkan terjadi peningkatan pengetahuan santri mengenai jenis-jenis sampah sebanyak 10% serta peningkatan pengalaman budidaya sayuran sebanyak 26,37%. Kegiatan pengabdian ini juga dapat mengurangi belanja sayuran sehari-hari hingga 50%.
Microsatellite Markers and Metabolite Profiles of Salt-Tolerant Rice: Inpari Unsoed 79 Agritan Rinanda Gandhi Ningrum Prasetia; Suprayogi Suprayogi; Ari Asnani; Eka Oktaviani; Isa Nuryana
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 2 (2022): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i2.57728

Abstract

Salinity is a challenge in crop production. High salinity affects soil osmotic pressure and the balance of nutrients that inhibit plant growth. In such case, utilization of salt-tolerant rice varieties could be an alternative. This study aims to identify microsatellite markers associated with salt tolerance, compare the Inpari Unsoed 79 Agritan variety with ten other rice genotypes based on microsatellite markers and determine the qualitative composition metabolites in Inpari Unsoed 79 Agritan associated with the plant response to salinity. This research was carried out at the Laboratory of Plant Breeding and Biotechnology Universitas Jenderal Soedirman and Indonesian Institute of Sciences, Bogor. This research used eleven rice varieties and ten microsatellite markers. The identification of microsatellite markers consisted of genomic DNA extraction, quantification and qualification of DNA, amplification of microsatellite DNA and data analysis. Metabolite profiling was conducted on Gas Chromatography-Mass Spectrometry (GC-MS) instrument. The results showed that microsatellite markers RM 241, RM 515, RM 519 and RM 528 differentiate the Inpari Unsoed 79 Agritan from the IR 29 genotype. Microsatellite markers RM 129 and RM 292 distinguished the Nona Bokra from the IR 29 genotypes. The genetic relationship of eleven rice genotypes resulted in two clusters. The GC-MS metabolite compounds in Inpari Unsoed 79 Agritan are β-Alanine and trimethylsilyl ester β-Alanine, a derivative compound of β-Alanine. These findings suggested that microsatellite markers RM 129, RM 292, RM 241, RM 515, RM 519 and RM 528 were associated with salt-tolerant in the seedling stage.
ANALISIS PROTEIN ISOLAT BAKTERI Escherichia coli BL 21 MENGGUNAKAN Sodium Dodecyl Sulphate – Polyacrylamide Gel Elektrophoresis (SDS-PAGE) Eka Oktaviani; Maya Ekaningtias
PENBIOS: JURNAL PENDIDIKAN BIOLOGI DAN SAINS Vol 4 No 01 (2019): PENBIOS: Jurnal Pendidikan Biologi dan Sains, Vol. 4, No. 1, Mei 2019 pISSN 2541
Publisher : Biology Education Department, Faculty of Teacher Training and Education, University of Nahdlatul Wathan Mataram - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Protein is a structural and functional component found in living organisms. This study aims to determine the quantity of protein obtained and the type of protein that was successfully extracted from the culture of Escherichia coli BL 21 (DE3). The research method uses exploratory descriptive, measurement of protein concentration by spectrophotometer and analysis of protein molecular weight by SDS-PAGE electrophoresis. The results of the study it was found that the total protein isolated from E. coli BL21 (DE3) bacterial culture had a concentration of 7.67 µg / µl, the control concentration was higher than the sample which was 9.33 µg / µl. The conclutions of the study it was found that the concentration of total protein isolated from E. coli BL21 (DE3) bacterial culture had a of 7.67 µg/µl, but the molecular weight and type of protein in total protein were still unknown.
Molecular Characterization of Rhizobacteria (Isolate EO-4) as Potential Solvent of Phosphate in-vitro from the Mangrove Ecosystem of Teluk Awur, Jepara-Indonesia Eka Oktaviani; Rejeki Siti Ferniah; Arina Tri Lunggani
PENBIOS: JURNAL PENDIDIKAN BIOLOGI DAN SAINS Vol 5 No 01 (2020): PENBIOS: Jurnal Pendidikan Biologi dan Sains, Vol. 5, No. 1, Mei 2020 pISSN 2541
Publisher : Biology Education Department, Faculty of Teacher Training and Education, University of Nahdlatul Wathan Mataram - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/penbios.v5i01.207

Abstract

Rejuvenation and conservation of mangrove ecosystems, especially the Teluk Awur mangrove ecosystem, Jepara Regency, can be done by using bacteria that are capable of supporting plant growth or called Plant Growth Promoting Rhizobacteria (PGPR). One of the mechanisms that support plant growth by the PGPR group is phosphate dissolving activity, because the phosphate in the soil is in the form of a compound that is difficult for plants to absorb. This study aims to determine the character of Rhizobacter isolates as a superior phosphate solvent in dissolving phosphates in-vitro from the Teluk Awur mangrove ecosystem, namely isolates coded EO-4. These isolates have similar microbiological and biochemical characters to the genus Enterobacter. Molecular characterization of isolates was carried out using the Polymerase Chain Reaction (PCR) method - 16S rRNA sequence analysis (comparing with 16S rRNA sequences in gene banks). The results showed that the phosphate-solubilizing rhizobacteria that were isolated had the same base pair percentage of 48% with Enterobacter pyrinus (access number NR_028875).
PARAMETER KUALITATIF MUTAN TANAMAN KENTANG (Solanum tuberosum L.) SETELAH IRADIASI SINAR GAMMA Siti Nurchasanah; Agung Prasetyo Fitrianto; Slamet Rohadi Suparto; Purwanto Purwanto; Eka Oktaviani
J-PEN Borneo : Jurnal Ilmu Pertanian Vol 5, No 2 (2022)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS BORNEO TARAKAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/jpen.v5i2.2806

Abstract

Kentang (Solanum tuberosum L.) merupakan jenis komoditi hortikultura yang menghasilkan umbi dari keluarga Solanaceae. Kebutuhan kentang terus meningkat setiap tahun sejalan dengan meningkatnya jumlah penduduk dan berkembangnya industri yang membutuhkan bahan baku kentang. Kentang banyak dibudidayakan di dataran tinggi di atas 800 m dpl, seperti Dieng, Kerinci, Pengalengan, dan Curup yang memiliki suhu 17ºC sampai dengan 20ºC sehingga tanaman kentang dapat berproduksi maksimal. Namun, di dataran tinggi mulai muncul ancaman lingkungan seperti kekeringan dan tanah longsor, sehingga mendorong para peneliti untuk mengembangkan tanaman kentang di dataran medium. Pengembangan varietas tanaman kentang untuk dataran medium terus dilakukan, salah satunya dengan induksi mutasi. Induksi mutasi dapat dilakukan dengan perlakuan bahan mutagen tertentu terhadap bagian tanaman seperti organ reproduksi. Iradiasi sinar gamma merupakan mutasi yang sering digunakan untuk menginduksi perubahan genetik di dalam sel somatik yang diturunkan, sehingga terjadi peningkatan viabilitas dan menghasilkan mutan baru. Penelitian ini bertujuan untuk mengetahui pengaruh radiasi sinar gamma terhadap berbagai variabel kualitatif 3 (tiga) varietas tanaman kentang. Perlakuan radiasi sinar gamma memberikan pengaruh yang berbeda dengan tanaman kontrol pada variabel bentuk daun, frekuensi daun menyimpang, kebiasaan tumbuh, bentuk umbi, dan sifat ketahanan terhadap penyakit. Mutan yang memberikan keunggulan dalam segi ketahanan terhadap penyakit adalah mutan D1-A11, D2- A11, D2-A4, D1-G1, D1-G2, D3-G11, D3-G12, dan D2-M11.Kata kunci : kualitatif, kentang, iradiasi gamma, dataran medium
Genetic Parameters, Inter-relationship Among Agronomic Traits and Dehulled Rice Morpho-Biochemical Profile of Promising Black Rice x Mentik Wangi Lines Eka Oktaviani; . Suprayogi
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.834-844

Abstract

A comprehensive understanding of the genetic parameters and the interrelationship among characters in the breeding population is crucial for selecting low amylose and high antioxidant black rice varieties. Meanwhile, dehulled rice morpho-biochemistry profile can be used to determine the grain quality of F6 and F7 lines of Black Rice x Mentik Wangi var. The objectives of this study were to determine the agronomic traits, figure up the genetic parameters, describe the relationship among agronomic characteristics of the F6 lines, and determine the morpho-biochemical profile of F7 dehulled rice. Agronomic traits showed a difference in each line. Genetic parameters in each trait showed various categories. Path analysis showed that the number of tillers affected the dry weight and grain weight per panicle, but the plant dry weight did not directly affect the weight of 1,000 grain. Directly, the weight of 1,000 grain was only significantly affected by the grain weight per panicle trait. The F7 lines had a difference in grain length and amylose content. The dehulled rice color of the two lines showed the combination of the two parents. Based on the student T-test conducted on F6 and F7 grain, there was no difference in antioxidant content between the two sample groups. The antioxidant activity of all lines was in the range between the antioxidant activities of the two checked varieties. Although further research is still needed, the lines have the potency to be developed as low amylose pigmented rice.
AUTHENTICATION OF THREE WAX APPLES CULTIVARS (SYZYGIUM SAMARANGENSE (BLUME) MERR. & L.M. PERRY) BASED ON MORPHOLOGICAL CHARACTER AND FRUIT METABOLITE PROFILE Annisa Nur Rachmah; Asri Febriana; Niken Kusumarini; Eka Oktaviani; Arnia Sari Mukaromah
Floribunda Vol. 7 No. 2 (2023): Floribunda April 2023
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v7i2.2023.409

Abstract

Wax apple is one of the superior fruits of Demak, especially cultivars 'Citra', 'Delima' and ‘Madu Deli Hijau'. Research on the wax apple of the three cultivars is still limited. Therefore, research on the authentication of wax apple cultivars based on morphological characters and metabolite profiles present in one location is important to do. The aims of this study were to identify morphological characters and analyze metabolite profiles, to analyze relationship and to identify morphological characters and metabolite marker profiles in the authentication activities of three wax apples cultivars. Sampling was in Boyolali Village, Demak Regency. The stages of this research included morphological characterization of stem, leaf, fruit and seed organs, phylogeny analysis with MVSP, metabolite profile analysis using GC-MS, and determination of morphological characters and metabolite profiles with PCA. The results showed that there were morphological diversity and metabolite profile variations of the three cultivars. Relationship analysis based on morphological characters and metabolite profiles resulted in different grouping patterns. 'Madu Deli Hijau' and 'Citra' are closely related based on morphological characters and a combination of morphological characters and metabolite profiles. Morphological characters in the authentication of the 'Citra' (dark red exocarp color), 'Delima' (bright red exocarp color, smooth and flat fruit surface, jagged fruit shape), in 'Madu Deli Hijau' (elongated leaf shape and exocarp is green with a pink tinge). Authentication of probable marker metabolite profiles on 'Citra', 'Delima' and 'Madu Deli Hijau' wax apples of 10, 7 and 5 compounds.
Hypersensitivity Test of Insect-Carried Bacteria in Chilli Crop at Banyumas Regency Agus Suroto; Endang Mugiastuti; Tarjoko Tarjoko; Eka Oktaviani; Muhammad Bahrudin
Gontor Agrotech Science Journal Vol. 9 No. 1 (2023): June 2023
Publisher : University of Darussalam Gontor, Ponorogo, East Java Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/agrotech.v9i1.9903

Abstract

As a horticultural crop with high economic and cultural values, the productivity of chili (Capsicum annum L.) fluctuates from time to time. This is influenced by changes in weather and climate which lead to the low resistance of chili plants to pests and diseases. Climate change can affect the existence of pests and diseases in plants and even reduce yields between 10-28% on a global scale. Bacteria, in association with plants, can be both pathogenic and non-pathogenic. While pests tend to have a negative relationship with plants because they can cause damage to plants. Banyumas Regency (Central Java) is one of the districts that contribute to the supply of chili which is important to increase production. This study aims to distinguish pathogenic and non-pathogenic bacteria, which are carried by insects in chili planting fields in 3 (three) endemic locations for pest attacks, namely Sumbang, Sokaraja, and Karanglewas Districts. The research was conducted from March to November 2021. As many as 83.33% of the total grown isolates did not show hypersensitive reactions while the others showed positive results. However, further research needs to be carried out for further research (characterization, identification, and other potential of the isolated insect-borne bacteria).
Co-Authors Afidani, Muthia Hanin Agung Prasetyo Fitrianto Agus Riyanto Alifia Khairunnisa Amalia, Dwi Ayu Lutfiana Annisa Nur Rachmah Ari Asnani Arina Tri Lunggani Arina Tri Lunggani Arnia Sari Mukaromah Asri Febriana Bahrudin, Muhammad Endang Mugiastuti Endang Mugiastuti Etik Wukir Tini Fauziah, Jihan Nur Isa Nuryana Isnaeni, Fenty Chakimatul Kurniawan, Ruly Eko Kusuma Leana, Ni Wayan Anik Maya Ekaningtias Muhammad Bahrudin Ni Wayan Anik Leana Niken Kusumarini Noor Farid Omer, Omer Ahmed Mohamed Prasmadji Sulistyanto Prasmaji Sulistyanto Priswanto Priswanto, Priswanto Purwanto Purwanto Purwanto Purwanto Purwanto Purwanto Purwanto, Purwanto Rejeki Siti Ferniah Rinanda Gandhi Ningrum Prasetia Siti Nurchasanah Slamet Rohadi Suparto Suprayogi Suroto, Agus Susanti, Retna Tarjoko Tarjoko Tarjoko Tarjoko Tarjoko Tarjoko, Tarjoko Totok Agung Dwi Haryanto Ulinnuha, Zulfa Wulansari, Nur Kholida