Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Indonesian Journal of Agricultural Science
Masdiar Bustamam
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
IDENTIFICATION OF A MAJOR QUANTITATIVE TRAIT LOCUS CONFERRING RICE BLAST RESISTANCE USING RECOMBINANT INBRED LINES Sobrizal, Sobrizal; Bustamam, Masdiar; Carkum, Carkum; Warsun, Ahmad; Human, Soeranto; Fukuta, Yoshimichi
Indonesian Journal of Agricultural Science Vol 11, No 1 (2010): April 2010
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Blast disease caused by Pyricularia oryzae is one of the limitingfactors for rice production world wide. The use of resistantvarieties for managing blast disease is considered as the mosteco-friendly approaches. However, their resistances may bebroken down within a few years due to the appearance of newvirulent blast races in the field. The objective of the presentstudy was to identify the quantitative trait locus (QTL) conferringresistance to blast disease using 126 recombinant inbred(RI) lines originated from a crossing of a durably resistant uplandrice genotype (Laka) and a highly susceptible rice accessioncultivar (Kencana Bali). The RI population was developedthrough a single seed descent method from 1997 to 2004.Resistance of the RI lines was evaluated for blast in an endemicarea of Sukabumi, West Java, in 2005. Disease intensity of theblast was examined following the standard evaluation systemdeveloped by the International Rice Research Institute (IRRI).At the same year the RI lines were analyzed with 134 DNAmarkers. Results of the study showed that one major QTL wasfound to be associated with blast resistance, and this QTL waslocated near RM2136 marker on the long arm of chromosome11. This QTL explained 87% of the phenotypic variation with37% additive effect. The map position of this QTL differedfrom that of a partial resistant gene, Pi34, identified previouslyon chromosome 11 in the Japanese durably resistant variety,Chubu 32. The QTL, however, was almost at the same positionas that of the multiple allele-resistant gene, Pik. Therefore, anallelic test should be conducted to clarify the allelic relationshipbetween QTL identified in this study and the Pik. The RI linesare the permanent segregating population that could be veryuseful for analysing phenotypic variations of important agronomictraits possibly owned by the RI lines. The major QTLidentified in this study could be used as a genetic resource inimprovement of rice varieties for blast resistance in Indonesia
Evaluation of Several Pup1-Lines of Rice (Oryza sativa L.) on Nutrient Solution and at the Field Prasetiyono, Joko; Suhartini, Tintin; Soemantri, Ida Hanarida; Tasliah, ,; Moeljopawiro, Sugiono; Aswidinnoor, Hajrial; Sopandie, Didy; Bustamam, Masdiar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.883 KB) | DOI: 10.24831/jai.v40i2.13200

Abstract

Phosphorus (P) deficiency is one of major problems in rice cultivation. Development of a tolerant variety to P deficient soil is expected to reduce the needs of P fertilizer. The aim of this research was to evaluate the agronomic performance of Pup1-introgression rice lines. This research consisted of two separate experiments, an evaluation on rice grown in nutrient solution in the greenhouse of ICABIOGRAD, Bogor; and field evaluation at upland rice screening site Taman Bogo, Lampung. The rice lines used were BC2 F3 derived from crossing of Situ Bagendit with Kasalath and NIL-C443, and from crossing of Batur with Kasalath and NIL-C443. Molecular analysis was conducted to verifiy the Pup1 introgression in the lines used and successfully confirmed the occurance of homozygote Pup1 segment in those lines. Evaluation of rice lines grown in nutrient solution was performed in a split plot factorial design, using the dose of Al (0 and 45 ppm Al3+) as main plots and dose of P (0.5 and 10 ppm P) as subplots. Field evaluation was performed in split plot design, with P fertilization (0 and 500 kg SP-18 ha-1) as main plots and BC2 F3 lines as the subplots. Evaluation using Yoshida nutrient solution showed that the BC2 F3 lines had greater total dry weight under low-P condition (37.5-112.5%), especially under Al-toxicity, compared to the respective parental varieties (Situ Bagendit and Batur). Field evaluation showed that the Pup1-introgression lines had greater shoot dry weight than the respective parental lines (10.5-74.82%). However there was no significnant effect of Pup1 introgression in terms of weight of filled grain.
Co-Authors , Tasliah Ahmad Warsun Carkum Carkum Didy Sopandie HAJRIAL ASWIDINNOOR Ida Hanarida Soemantri Joko Prasetiyono Sobrizal Sobrizal Soeranto Human SUGIONO MOELJOPAWIRO Tintin Suhartini Yoshimichi Fukuta