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Patogenisitas Beberapa Isolat Cendawan Entomopatogen terhadap Coptotermes curvignathus Holmgren dan Schedorhinotermes javanicus Kemmer Harahap, Idham Sakti
Jurnal Agro Teknologi Tropika Vol 2, No 1 (2013)
Publisher : Jurnal Agro Teknologi Tropika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengendalian hayati rayap Coptotermes curvignathus dan Schedorhinotermes javanicus dengan menggunakan beberapa isolat cendawan entomopatogen merupakan salah satu alternatif untuk pengendalian rayap tanah yang ramah lingkungan. Penelitian ini bertujuan untuk mempelajari patogenisitas cendawan entomopatogen isolat Indonesia Metarhizium anisopliae, M. brunneum, B. bassiana, dan Myrothecium roridum dan menguji keefektifannya terhadap rayap tanah C. curvignathus dan S. Javanicus. Uji patogenisitas beberapa isolat cendawan entomopatogen terhadap C. curvignathus dan S. javanicus dilakukan dengan pencelupan rayap ke dalam suspensi konidia, masing-masing dengan kerapatan 0,105,5x105,106,5x106,dan 107 konidia per ml, setiap perlakuan diulang lima kali. Sporulasi pada tubuh rayap dihitung dari rayap C. curvignathus yang telah mati pada uji patogenisitas. Data yang diperoleh dianalisis dengan sidik ragam dan uji lanjut selang ganda Duncan pada taraf nyata 5%. Hubungan kerapatan konidia dengan mortalitas dan waktu aplikasi dengan mortalitas diolah dengan analisis probit. Berdasarkan hasil analisis probit dapat diperoleh nilai LC dan LT. Patogenisitas M. brunneum terhadap rayap S. javanicus dan C. curvignathus lebih tinggi dibandingkan dengan M. anisopliae, Beauveria bassiana, dan Myrothecium roridum. Sporulasi M. brunneum lebih tinggi dari pada M. roridum maupun B. bassiana dan sporulasi terendah terdapat pada B. bassiana. Viabilitas M. brunneum tidak berbeda nyata dengan M. roridum dan viabilitas B. bassiana berbeda nyata dengan M. roridum.Kata kunci : cendawan entomopatogen, M. brunneum, rayap
SIMTOMATOLOGI DAN WAKTU KEMATIAN RAYAP Macrotermes gilvus Hagen (ISOPTERA: FAMILI TERMITIDAE) SETELAHINFEKSI CENDAWAN Metarhizium brunneum Petch Sayuthi, Muhammad; Santoso, Teguh; Harahap, Idham Sakti; Kastosuwondo, Utomo
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.801 KB) | DOI: 10.14203/beritabiologi.v10i6.1941

Abstract

The potential of entomopathogenic fungus Metarhizium brunneum Petch as biocontrol agent to termite Macrotermes gilvus Hagen has been tested in the laboratory.The purpose of this research is to study the symptomatology and lethal time of the termite M. gilvus fungus after infection by M. brunneum. The density of conidia at 1.21xlO6/mL showed as effective concentration in causing M. gilvus mortality, when compared to 1.08><106conidia/mL, and control. The infection stage of M. brunneum on the host until the death of its host occurred on day 2,and the sporulation of M. brunneum on the surface of the host integument occur on day 4. The entire surface of M. gilvus was covered by the mycelium and conidia of M. brunneum on day 7. Lethal time 50% of termite population of M. gilvus (LT50) was achieved in 5 days(5.14), and LT95 achieved on day 10 (10.03).
DIVERSITY OF SUBTERRANEAN TERMITES (BLATTODEA: TERMITOIDEA) ON VARIOUS TYPES HABITAT AROUND IPB UNIVERSITY CAMPUS, BOGOR, INDONESIA Mubin, Nadzirum; Sakti Harahap, Idham; .,, Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 19 No. 2 (2019): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.219158-169

Abstract

Termites are social insects that play an important role in ecosystem. Habitat is a crucial factors determining diversity of termites. IPB University campus has quite diverse habitat types. In this research, we study the diversity and abundance of termite species around IPB University campus. Two methods used for data collection, i.e. baiting system with pine wood and transect survey within 10 x 10 m area. Six species of termites found belongs to two families, Termitidae (Macrotermes gilvus, Odontotermes javanicus, Microtermes insperatus, and Capritermes mohri) and Rhinotermitidae (Schedorhinotermes javanicus and Coptotermes curvignathus). The most diverse with the high abundance of these termites are found in the  rubber trees area, Faculty of Fisheries (FPI) and the forest behind the main administration building, Rectorate (REK) because this area provides an abundant source of cellulose as a food source for termites. While the building areas tend to have a lower diversity and abundance index because food sources for termites are not always available due to human activities. Non-metric multidimensional scaling analysis shows that the termites species composition is different between study area.
The effectiveness of kaffir lime leaf essential oil as a fumigant for Tribolium castaneum (Herbst) Khalifah, Alif; Harahap, Idham Sakti; Dadang, Dadang
Jurnal Penelitian Tanaman Industri Vol 27, No 2 (2021): December 2021
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v27n2.2021.51-57

Abstract

Pests are often a problem with stored post-harvest products. One of the pests that often attack post-harvest products in storage is Tribolium castaneum (Herbst). The most common warehouse pest control effort is fumigation. Unwise and inappropriate application of fumigants can cause various negative impacts, such as pest resistance. Therefore, it is necessary to develop alternative control strategies that are more environmentally friendly through essential oils. The purpose of this study was to determine the effectiveness of kaffir lime (Citrus hystrix) essential oil as a botanical fumigant against T. castaneum that held at SEAMEO Biotrop Bogor. Kaffir lime leaf essential oil (KLLEO) is obtained by distillation. Tests in this study include the toxicity test of essential oils and essential oil fractions, resistance tests, and test the effect of essential oils on larval development. KLLEO n-hexane fraction caused high mortality of T. castaneum imago after 72 hours of fumigation. The LD95 KLLEO value in imago was 1.14 ml / l of air. MADJP also had high repellency activity against imago. The lowest dose of 0.03 ml/l air and the highest dose of 1.14 ml/l air showed a degree of 95.5 and 100% reliability, respectively, 6 hours after treatment. Chemical composition analysis using GCMS showed that citronella was the most dominant compound at 79.05%.Keywords: Citrus hystrix, fumigation, post-harvest pests AbstrakKEEFEKTIFAN MINYAK ATSIRI DAUN JERUK PURUT SEBAGAI FUMIGAN Tribolium castaneum (HERBST)Serangga hama seringkali menjadi masalah pada produk pascapanen yang disimpan. Salah satu hama yang sering menyerang produk pascapanen di gudang penyimpanan adalah Tribolium castaneum (Herbst). Upaya pengendalian hama gudang yang paling umum dilakukan adalah dengan fumigasi. Aplikasi fumigan yang tidak bijaksana dan tidak tepat dapat menimbulkan berbagai dampak negatif, seperti terjadinya resistensi hama. Oleh karena itu, perlu dikembangkan strategi pengendalian alternatif yang lebih ramah lingkungan melalui penggunaan minyak atsiri. Tujuan penelitian ini untuk mengetahui keefektifan minyak atsiri daun jeruk purut (Citrus hystrix) sebagai fumigan nabati terhadap T. castaneum yang dilaksanakan di SEAMEO Biotrop Bogor. Minyak atsiri daun jeruk purut (MADJP) diperoleh melalui distilasi. Pengujian dalam penelitian ini meliputi uji toksisitas minyak atsiri dan fraksi minyak atsiri, uji repelensi, dan uji pengaruh minyak atsiri terhadap perkembangan larva. Fraksi n-heksana MADJP menyebabkan kematian yang tinggi pada imago T. castaneum setelah 72 jam fumigasi. Nilai LD95 MADJP pada imago sebesar 1,14 ml/l udara. MADJP juga memiliki aktivitas repelensi yang tinggi terhadap imago. Dosis terendah 0,03 ml/l udara dan dosis tertinggi 1,14 ml/l udara menunjukkan derajat keandalan berturut-turut 95,5 dan 100% pada 6 jam setelah perlakuan. Analisis komposisi kimia menggunakan GCMS menunjukkan bahwa sitronela merupakan senyawa yang paling dominan sebesar 79,05%.Kata kunci : Citrus hystrix, fumigasi, hama pasca panen, sitronela
Fumigant and Repellent Effects of Essential Oil Fractions of Mentha piperita against Tribolium castaneum (Coleoptera: Tenebrionidae) Syam, Sunaryo; Harahap, Idham Sakti; Dadang, NFN
Buletin Penelitian Tanaman Rempah dan Obat Vol 28, No 2 (2017): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v28n2.2017.181-190

Abstract

Tribolium castaneum is one of the important stored-product pests in tropical countries. Fumigation with phosphine is the most commonly method to control T. castaneum. However, long-term application of phosphine will cause resistance. The aims of this research were to study the effects of fumigant and repellent of peppermint oil Mentha piperita fraction against T. castaneum, and to identify the compounds contained in the active fraction. This research was conducted in Entomology Laboratory Seameo Biotrop and Forensic Laboratory of Indonesian Police Headquarters Jakarta, from January to October 2016. Peppermint oil was extracted by distillation from the leaves of M. piperita. Peppermint oil was fractionated by counter-current distribution method using three solvents: n-hexane, ethyl acetate, and methanol. Then, the three fractions were tested as fumigant and repellent against T. castaneum. The active fraction of essential oils were identified by GC-MS. The n-hexane fraction of peppermint (HFP) was the active fraction caused mortality on larvae and adults of  T. castaneum at 72 hours after fumigation. The LC95 values for the adults and  larvae was 1.9 % and 9.43 % , respectively. Sublethal concentration of HFP showed inhibitory activity on larvae development. Percentage of pupae transformed from treated larvae was 48.67-74.91 % and percentage of adults emerged was 9.12-16.59 %. The repellent activity of HFP on adults of T. castaneum was 86.7 % at 72 hours after treatment. The dominant compounds of HFP was β-pinene, limonene, isopulegol, menthone, isomenthon, menthol, pulegone, and trans-carane. The HFP or its compounds was potential to be develoved as botanical insecticides.
Perilaku Kunjungan dan Efisiensi Penyerbukan Heterotrigona itama (Cockerell) dan Tetragonula laeviceps (Smith) (Hymenoptera: Apidae) pada Labu Siam Qurrotu A’yunin; Aunu Rauf; Idham Sakti Harahap
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 3 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.66 KB) | DOI: 10.18343/jipi.24.3.247

Abstract

Main insect pollinator of chayote in the neotropics is the stingless bees. In Indonesia, there is no information available on chayote pollination. Research was conducted with the objective to study the flowering phenology of chayote and the role of two specieses of stingless bees Heterotrigona itama (Cockerell) and Tetragonula laeviceps (Smith) (Hymenoptera: Apidae) on chayote pollination. Measurements included the number of staminate and pistillate flowers, volume of nectar and sugar concentration, floral handling time and visitation rate, and fruit set. Chayote plants produced more staminate flowers than pistillate flowers, with the ratio of 18:1. There was no difference in nectar volume and sugar concentration between staminate and pistillate flowers. H. itama and T. laeviceps visited both type of flowers, with the peak of daily visitation were occurred at 08:30-10:30 am local times. There were significant differences in the floral handling time and foraging rate of H. itama and T. laeviceps. Pollen load was significantly higher in H. itama (2137.50±184.49 grains) than those in T. laeviceps (1675.00±110.47 grains). H. itama seemed to deposite more pollen (14.1±4.1 grains) on stigma than T. laeviceps (9.8±3.1 grains). Flowers prevented from insect visits resulted in zero fruit set. Single flower visit by H. itama and T. laeviceps resulted in 60 and 40% fruit set, respectively. Higher percent of fruit set (80-85%) was noticed in open-pollination, suggesting that multiple visits by insect pollinators might increase the chances of successful pollination.
Bacillus endophyticus: Symbiotic Bacterium in Subterranean Termites Intestine (Blattodea: Termitoidae) from Bogor, Indonesia Nadzirum Mubin; Giyanto Giyanto; Idham Sakti Harahap
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.191

Abstract

Rayap merupakan serangga sosial yang berperan penting dalam perputaran siklus nutrisi. Di dalam sistem pencernaan rayap, terdapat simbion yang membantu proses degradasi selulosa. Penelitian ini bertujuan mengisolasi bakteri simbion yang terdapat di dalam saluran cerna rayap tanah. Penelitian diawali dengan koleksi rayap tanah di Kampus IPB University, diikuti isolasi bakteri simbion dari saluran cerna belakang (proktodeum) yang kemudian diidentifikasi berdasarkan morfologi, fisiologi, dan molekuler menggunakan gen 16S rRNA. Enam rayap tanah yang diperoleh adalah Macrotermes gilvus, Odontotermes javanicus, Microtermes insperatus, dan Capritermes mohri (Famili Termitidae); Schedorhinotermes javanicus dan Coptotermes curvignathus (Famili Rhinotermitidae). Dari enam rayap diperoleh 43 isolat dan satu isolat yang memiliki karakter umum. Isolat bakteri kode 8A_27F berwarna putih, elevasi seperti tombol dengan tepian licin. Dari uji fisiologis, isolat ini termasuk ke dalam bakteri Gram positif, berspora, dan bersifat aerob. Identifikasi dengan gen 16S rRNA menunjukkan bahwa isolat bakteri tersebut memiliki tingkat kemiripan sebesar 98% dengan spesies Bacillus endophyticus. Kata kunci: bakteri, rayap, 16S rRNA, simbion, proktodeum
The effectiveness of kaffir lime leaf essential oil as a fumigant for Tribolium castaneum (Herbst) Alif Khalifah; Idham Sakti Harahap; Dadang Dadang
Jurnal Penelitian Tanaman Industri Vol 27, No 2 (2021): December 2021
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v27n2.2021.51-57

Abstract

Pests are often a problem with stored post-harvest products. One of the pests that often attack post-harvest products in storage is Tribolium castaneum (Herbst). The most common warehouse pest control effort is fumigation. Unwise and inappropriate application of fumigants can cause various negative impacts, such as pest resistance. Therefore, it is necessary to develop alternative control strategies that are more environmentally friendly through essential oils. The purpose of this study was to determine the effectiveness of kaffir lime (Citrus hystrix) essential oil as a botanical fumigant against T. castaneum that held at SEAMEO Biotrop Bogor. Kaffir lime leaf essential oil (KLLEO) is obtained by distillation. Tests in this study include the toxicity test of essential oils and essential oil fractions, resistance tests, and test the effect of essential oils on larval development. KLLEO n-hexane fraction caused high mortality of T. castaneum imago after 72 hours of fumigation. The LD95 KLLEO value in imago was 1.14 ml / l of air. MADJP also had high repellency activity against imago. The lowest dose of 0.03 ml/l air and the highest dose of 1.14 ml/l air showed a degree of 95.5 and 100% reliability, respectively, 6 hours after treatment. Chemical composition analysis using GCMS showed that citronella was the most dominant compound at 79.05%.Keywords: Citrus hystrix, fumigation, post-harvest pests AbstrakKEEFEKTIFAN MINYAK ATSIRI DAUN JERUK PURUT SEBAGAI FUMIGAN Tribolium castaneum (HERBST)Serangga hama seringkali menjadi masalah pada produk pascapanen yang disimpan. Salah satu hama yang sering menyerang produk pascapanen di gudang penyimpanan adalah Tribolium castaneum (Herbst). Upaya pengendalian hama gudang yang paling umum dilakukan adalah dengan fumigasi. Aplikasi fumigan yang tidak bijaksana dan tidak tepat dapat menimbulkan berbagai dampak negatif, seperti terjadinya resistensi hama. Oleh karena itu, perlu dikembangkan strategi pengendalian alternatif yang lebih ramah lingkungan melalui penggunaan minyak atsiri. Tujuan penelitian ini untuk mengetahui keefektifan minyak atsiri daun jeruk purut (Citrus hystrix) sebagai fumigan nabati terhadap T. castaneum yang dilaksanakan di SEAMEO Biotrop Bogor. Minyak atsiri daun jeruk purut (MADJP) diperoleh melalui distilasi. Pengujian dalam penelitian ini meliputi uji toksisitas minyak atsiri dan fraksi minyak atsiri, uji repelensi, dan uji pengaruh minyak atsiri terhadap perkembangan larva. Fraksi n-heksana MADJP menyebabkan kematian yang tinggi pada imago T. castaneum setelah 72 jam fumigasi. Nilai LD95 MADJP pada imago sebesar 1,14 ml/l udara. MADJP juga memiliki aktivitas repelensi yang tinggi terhadap imago. Dosis terendah 0,03 ml/l udara dan dosis tertinggi 1,14 ml/l udara menunjukkan derajat keandalan berturut-turut 95,5 dan 100% pada 6 jam setelah perlakuan. Analisis komposisi kimia menggunakan GCMS menunjukkan bahwa sitronela merupakan senyawa yang paling dominan sebesar 79,05%.Kata kunci : Citrus hystrix, fumigasi, hama pasca panen, sitronela
Fumigant and Repellent Effects of Essential Oil Fractions of Mentha piperita against Tribolium castaneum (Coleoptera: Tenebrionidae) Sunaryo Syam; Idham Sakti Harahap; NFN Dadang
Buletin Penelitian Tanaman Rempah dan Obat Vol 28, No 2 (2017): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v28n2.2017.181-190

Abstract

Tribolium castaneum is one of the important stored-product pests in tropical countries. Fumigation with phosphine is the most commonly method to control T. castaneum. However, long-term application of phosphine will cause resistance. The aims of this research were to study the effects of fumigant and repellent of peppermint oil Mentha piperita fraction against T. castaneum, and to identify the compounds contained in the active fraction. This research was conducted in Entomology Laboratory Seameo Biotrop and Forensic Laboratory of Indonesian Police Headquarters Jakarta, from January to October 2016. Peppermint oil was extracted by distillation from the leaves of M. piperita. Peppermint oil was fractionated by counter-current distribution method using three solvents: n-hexane, ethyl acetate, and methanol. Then, the three fractions were tested as fumigant and repellent against T. castaneum. The active fraction of essential oils were identified by GC-MS. The n-hexane fraction of peppermint (HFP) was the active fraction caused mortality on larvae and adults of  T. castaneum at 72 hours after fumigation. The LC95 values for the adults and  larvae was 1.9 % and 9.43 % , respectively. Sublethal concentration of HFP showed inhibitory activity on larvae development. Percentage of pupae transformed from treated larvae was 48.67-74.91 % and percentage of adults emerged was 9.12-16.59 %. The repellent activity of HFP on adults of T. castaneum was 86.7 % at 72 hours after treatment. The dominant compounds of HFP was β-pinene, limonene, isopulegol, menthone, isomenthon, menthol, pulegone, and trans-carane. The HFP or its compounds was potential to be develoved as botanical insecticides.
Komposisi Rayap Dapat Menentukan Tingkat Ketergangguan Habitat : Studi Kasus di Kabupaten Dharmasraya Provinsi Sumatera Barat Sri Heriza; Damayanti Buchori; Idham Sakti Harahap; Nina Maryana
Jurnal Ilmu Lingkungan Vol 20, No 3 (2022): July 2022
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.20.3.678-684

Abstract

Transformasi habitat seperti pengalihan hutan alam ke bentuk habitat lain mempengaruhi aktivitas kehidupan organisme penting di dalamnya. Rayap merupakan organisme penting yang mempunyai peran sebagai dekomposer. Oleh sebab itu perlu dikaji dari komposisinya pada habitat-habitat dengan tingkat ketergangguan yang bervariasi. Penelitian ini bertujuan mempelajari komposisi rayap pada beberapa tingkat ketergangguan habitat. Penelitian ini dilakukan pada empat tipe penggunaan lahan di Kabupaten Dharmasraya Provinsi Sumatera Barat, yaitu hutan alam, hutan kebun, perkebunan kelapa sawit dan permukiman. Penelitian dilakukan dengan cara observasi di lapangan dan identifikasi spesies rayap di laboratorium. Untuk observasi, dilakukan di tiap habitat dengan petak pengamatan berukuran 50 m x 10 m. Satu petak pengamatan dibagi dalam sub petak pengamatan dengan ukuran 5 m x 5 m sehingga diperoleh 20 sub petak pengamatan. Setiap sub petak pengamatan dilakukan survei selama 30 menit, sehingga total untuk satu petak pengamatan adalah 600 menit atau 10 jam. Identifikasi rayap dilakukan di laboratorium dengan berpedoman pada beberapa kunci identifikasi spesies rayap. Pada penelitian ini ditemukan 14 spesies rayap pada beberapa habitat di Kabupaten Dharmasraya. Hasil analisis terhadap feeding group, indeks keanekaragaman, indeks kekayaan dan dominansinya menunjukkan habitat hutan alam dan hutan kebun cenderung memiliki kedekatan komposisi rayap yang lebih dekat daripada komposisi rayap yang ada di perkebunan kelapa sawit dan permukiman, kemudian komposisi rayap di perkebunan kelapa sawit dan permukiman memiliki tingkat kesamaan yang lebih dekat pula.ABSTRACTThe transformation of habitats such as natural forests to other forms of habitat affects the life activities of important organisms in them. Termites are important organisms that have a role as decomposers. Therefore, it is necessary to study its composition in habitats with varying levels of disturbance. This study aims to study the composition of termites at several levels of habitat disturbance. This research was conducted on four types of land use in Dharmasraya Regency, West Sumatra Province, namely natural forest, secondary forest, oil palm plantation, and settlement. The research was conducted by observing in the field and finding termite species in the laboratory. For observations, conducted in each habitat with an observation plot measuring 50 m x 10 m. One observation plot in the observation sub-plot with a size of 5 m x 5 m in order to obtain 20 observation sub-plots. Each sub-plot was observed for 30 minutes, so that the total observations in one plot were 600 minutes or 10 hours. Termite identification is carried out in the laboratory based on several key termite discoveries. In this study, 14 species of termites were found in several habitats in Dharmasraya Regency. The results of the analysis of food groups, diversity index, richness index and dominance showed that natural forest and secondary forest tended to have closer proximity than the composition of termites in oil palm plantations and settlement and the composition of termites in oil palm plantations and settlement had high levels of even closer similarities.