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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI)

. Giyanto

Department Of Plant Protection, Faculty Of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680

Author-ID : 209322

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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POTENSI BAKTERI ENDOFIT DALAM MENEKAN PENYAKIT LAYU STEWART (PANTOEA STEWARTII SUBSP. STEWARTII) PADA TANAMAN JAGUNG Rahma, Haliatur; Zainal, Aprizal; Suharman, Memen; Sinaga, Meity S; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 14 No. 2 (2014): September, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Potential of endophytic bacteria to control stewart wilt disease (Pantoea stewartii subsp. stewartii) in maize. The purpose of this study was to explore endophytic bacteria from seedling, maize roots and grass roots as well as to test the ability of endophytic bacteria which could potentially suppress stewart wilt disease development in maize. Characterization of endophytic bacteria as biocontrol agents including: do not induce HR on tobacco, synthesize IAA, dissolve phosphate, produce siderophores, and antibiotic to Pantoea stewartii subsp. stewartii (Pnss). The results of research shoed 17 isolates of endophytic bacteria potentially as candidate biocontrol agents. Nine isolates were able to produce IAA, siderofores and phosphatase; two isolates produce IAA and phosphatase; six isolates produce IAA. Six isolates ie: AR1, AJ34, AJ15, AJ19, and AJ14 AN6, can increase maize plant resistance and suppress stewart wilt disease severity with a range of 48.95-55.60%.

KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO Mutaqin, Kikin Hamzah; ., Giyanto; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.

POTENSI BAKTERI ENDOFIT AKAR UBI JALAR (IPOMOEA BATATAS L.) ASAL KABUPATEN SORONG PAPUA BARAT SEBAGAI AGENSIA BIOKONTROL MELOIDOGYNE SPP. ., Tuminem; Sinaga, Meity S; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 15, No 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Potency of sweetpotato (Ipomoea batatas L.) root endophytic bacteria from Sorong District West Papua as biocontrol agent of Meloidogyne spp. Root knot nematodes/RKN, Meloidogyne spp. is one of the important pathogens in sweet potato plant. The disease incidence rate by the RKN on sweetpotato crop in Sorong District reached 88.77%. This study aims to get the sweet potato root endophytic bacteria that have potential as biocontrol agents against Meloidogyne spp. Endophytic bacteria was isolated from the roots of healthy sweet potato sampled from Sorong District, West Papua Province. Isolation and selection of bacteria using TSA media. Selected bacterial isolates, which were non-pathogenic to plants and humans then were identified with PCR technique using universal primer 63-F / 1387-R. The ability of bacteria to produce the lipase enzyme was selected using the media NB agar and rhodamine B. The protease enzyme-producing bacteria were selected using skim milk media. The chitinase enzyme-producing bacteria were selected using the colloidal chitin media. Production of cyanide was detected using filter paper soaked in a solution of CDS. The effectiveness of culture filtrate of bacteria as biocontrol agents was measured based on the percentage of 2nd juvenile mortality and egg hatching of Meloidogyne spp. Four isolates of endophytic bacteria, that were Enterobacter sp EAS (1a), Enterobacter sp. EAS (3a) Enterobacter ludwigii EAS (4), and Burkholderia cepacia EAS (6) produced lipase and protease. In addition, B. cepacia EAS (6) also produced chitinase. Those isolates caused mortality of the 2nd juvenile 81.4 to 95.2% and inhibited the egg hatching of Meloidogyne spp. 53.13 to 81.92%.

KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Sanjaya, Wilhelmus Terang Arga; Giyanto, .; Widyastuti, Rahayu; Santosa, Dwi Andreas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or ÃŽÂ²-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: ÃŽÂ²-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau ÃŽÂ²-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.

Pelapisan Benih Menggunakan Bakteri Probiotik untuk Mempertahankan Viabilitas Benih Jagung Manis (Zea mays saccharata Sturt.) selama Penyimpanan Sarah Desmia Muchtar; Eny Widajati; . Giyanto
Buletin Agrohorti Vol. 1 No. 4 (2013): Oktober 2013
Publisher : Departemen Agronomi dan Hortikultura

Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pelapisan benih menggunakan bakteri Bacillus subtilis, Serratia marcescens, dan Pseudomonas kelompok fluorescens terhadap viabilitas dan daya simpan benih jagung manis (Zea mays saccharataSturt.). Penelitian disusun dengan rancangan petak tersarang (Nested Design) dengan petak utama adalah periode simpan minggu ke 0, 3, 6, 9, 12, 15, 18, 21, dan 24 dan anak petak adalah perlakuan coating dengan bakteri Bacillus subtilis, Serratia marcescens, Pseudomonas kelompok fluorescens, dan kontrol. Hasil penelitian menunjukkan adanya interaksi yang sangat nyata antara perlakuan coating benih dan periode simpan terhadap daya berkecambah, berat kering kecambah normal, kadar air, dan populasi bakteri. Perlakuan coating benih dan periode simpan menunjukkan interaksi yang nyata terhadap indeks vigor benih. Tolok ukur kecepatan tumbuh benihhanya dipengaruhi oleh faktor tunggal periode simpan. Benih yang dilapisi bakteri menghasilkan nilai berat kering kecambah normal yang nyata lebih baik daripada benih tanpa coating. Benih tanpa coating memiliki nilai daya berkecambah sebesar 56.7% pada periode simpan 24 minggu. Benih yang dilapisi bakteri Bacillus subtilis dapat mempertahankan daya berkecambah hingga 64.0% sampai periode simpan 24 minggu, sedangkan Serratia marcescens mampu mempertahankan daya berkecambah hingga 60.0% . Berdasarkan tolok ukur daya berkecambah, pelapisan benih menggunakan bakteri Bacillus subtilis dan Serratia marcescens merupakan perlakuan pelapisan benih yang potensial untuk lebih dikembangkan. Bakteri Bacillussubtilis lebih mampu bertahan hidup selama penyimpanan dibanding dengan bakteri Serratia marcescens maupun Pseudomonas fluorescens. Populasi bakteri Bacillus subtilis sampai dengan periode simpan 24 minggu adalah 14.2 × 104 cfu g-1.

Aplikasi Bakteri dalam Perlakuan Seed Coating untuk Mempertahankan Viabilitas dari Benih Cabai (Capsicum annuum L.) yang Sehat Herliyana Indahwardani; Eny Widajati; . Giyanto
Buletin Agrohorti Vol. 5 No. 1 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Penelitian ini bertujuan mengetahui pengaruh perlakuan pelapisan benih menggunakan bakteri Bacillus subtilis, Pseudomonas keompok. fluorescens dan Serratia marcescens terhadap viabilitas benih cabai (Capsicum annuum L.) selama di penyimpanan. Penelitian ini mengunakan rancangan petak tersarang dengan tiga ulangan. Petak utama adalah periode simpan (0, 3, 6, 9, 12, 15, 18, 21 dan 24 minggu) dan anak petak adalah perlakuan coating dengan bakteri tertentu (Bacillus subtilis, Pseduomonas kelompok fluorescens, Serratia marcescen dan kontrol). Benih cabai IPB C5 masih memiliki viabilitas yang cukup baik hingga akhir penyimpanan, ditunjukkan dengan nilai daya berkecambah sebesar 77.33%. Perlakuan kontrol dan coating menggunakan bakteri menunjukkan nilai daya berkecambah, indeks vigor, kecepatan tumbuh dan bobot kering kecambah normal yang tidak berbeda nyata. Ketiga bakteri yang digunakan sebagai pelapis masih dapat hidup sampai periode simpan 24 minggu dengan populasi 5.89 x 104 cfu g-1 untuk Pseudomonas kelompok fluorescens, 4.79 x 104 cfu g-1 untuk Bacillus subtilis dan 1.70 x 104 cfu g-1 untuk Serratia marcescens.

Kajian pemanfaatan limbah organik cair untuk pembiakan masal agens antagonis pseudomonas flourescens serta uji potensinya sebagai bio-pestisida . Giyanto; Efi Toding Tondok
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1989.032 KB)

Pseudomonas fluorescens has been well known as biological control agent for plant diseases control. The ;:>roblem to apply the agents widely in the field or in the level of farmer is limited technology of mass production Nith low cost, due to the simple technology of propagation has not been yet available. The objective of this research is to study the potency of liquid organic wastes as media for mass production of P. f/uorescens and to formulate them as bio-pesticide. The results showed that modification of coconut water to pH of 7.0 could be used as media for growing P. flourescens. The P. fluorescens also could grow well in livestock liquid waste by adding 10% meat extract. On the other hand, the liquid tofu waste and liquid compost waste became good media for growing of P. fluorescens by addition of 10°/o meat extract and 1.2S0/o sugar. Tetes tebu will be very good media for P. f/uorescens at S% final concentration and by adding of 10°/o meat extract and 2.S0/o of sugar. The P. fluorescens showed high antagonistic effect to Ralstonia solanacearum and Sclerotium rolfsii in all of modified liquid organic wastes media. Survival and antagonisctic activity of P. fluorescens in modified organic liquid wastes stored at S°C or room temperature were 12 weeks. In vivo antagonistic and plant growth promoting activity showed that P. fluorescens grown in liquid organic waste suppressed the incidence of stem rot diseases caused by Sclerotium rolfsii and increased the vigor of plant growth on watermelon. Formulation of the P. Fluorescens grown in modified coconut water gave the best performance of P. fluorescens in supppressing of plant diseases and inducing plant growth. The product of BeMOR(e) (beneficial microorganism) from the result of this research will be proposed to be patented.

EVALUASI KEMURNIAN GENETIK DENGAN MARKA MIKROSATELIT DAN APLIKASI RIZOBAKTERIA UNTUK MENINGKATKAN PRODUKSI DAN MUTU BENIH JAGUNG HIBRIDA Memen Surahman; . Giyanto; Andi Takdir; Awaludin Hipi
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1248.379 KB)

One effort to improve of high-quality of maize seed were the development and application of methods for genetic quality testing, such as SSR marker. Another effort was used of rhizobacteria for increased the availability of nutrients, especially P in the soil. The aim of this study were: 1) Microsatellite markers specific to male and female parents of maize hybrid; 2) Seed genetic purity by using molecular marker; 3) Rhizobacteria that could increase the physiological quality of maize hybrid seed; and 4) Rhizobacteria that could increase the growth of plant and efficiency of fertilizer P. The result showed that: 1) From five markers tested, three markers namely phi96100, phi328175 and phi072 produced polymorphic bands and capable to distinguish parental lines of two maize hybrids. Microsatellite marker phi96100 was specific used for testing genetic purity of cv.Bima-4 and phi072 for cv.Bima-3. While phi328175 was specific markers to both hybrids maize. The test of cv. Bima-3 and Bima-4 indicated that genetic purity of both varieties were 97.5% and 80% respectivelly; 2) Isolates B28 and B46 could increase IV 19% and 22% respectively, and had a high speed of growth compared controls; 3). Isolates B46, B42, B13, P14, P31, AB2, AB3, AB11, ATS4, and ATS5 could increased of germination compared to control; 4) Treatment of rhizobacteri significantly affects on plant height 2, 4 and 6 week after planting (WAP); 5) P fertilizer dosage were not significantly influenced on the number of leaves at 2 and 4 WAP, but significant at 6 WAP. Isolate of B28, B42 and ATS4 were potential for increased of plant growth.

Pengaturan Arsitektur Tanaman untuk Menyeimbangkan Sink dan Source serta Meningkatkan Pertumbuhan dan Hasil Kacang Koro Pedang Abdullah Sarijan; Memen Surahman; Asep Setiawan; Giyanto Giyanto
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

The research to improve the growth, production, and seed quality of Jack Bean (Canavalia ensiformis L.) through pruning, which was carried out from May to October 2016 in Purwasari Village, Dramaga, Bogor Regency and continued by seed testing at the Seed Testing Laboratory, Bogor Agricultural University in December 2016. The research was designed in a randomized block design (RBD) with eight treatment of stem and branch pruning and one treatment without pruning as a control, i.e. without pruning of branches and stems (P1), pruning on the 10th stem nodes (P2 ), pruning on the 11th stem nodes (P3), pruning on the 5th branch nodes (P4), pruning on the five branch nodes and the 10th stem nodes (P5), pruning on the 5th branch nodes and stem nodes to -11 (P6), pruning on the 6th branch nodes (P7), pruning on the 6th branch nodes and 10th stem nodes (P8), and pruning on the 6th branch nodes and 11th stem nodes (P9). The results showed that pruning affected some of the results of the study, but was not able to increase the production of Jack Bean. Pruning treatment on the 5th branch nodes and 10th stem nodes (P5) produced the highest seed production (3.4 tons ha-1), the physical quality of the seeds produced was classified as moderate with a weight of 1000 seeds between 1273.3-1291.7 g. The physiological quality of the seed is moderate with maximum growth potential 84.0-90.7%, germination percentage 73.3-82.7%, germination speed 9.1-116.1% / etmal, germination uniformity 40.0-49 , 3% and vigor index of 14.0-20.0%. Keywords: germination rate, inflorescence, oas bakol, pod

Bacillus endophyticus: Symbiotic Bacterium in Subterranean Termites Intestine (Blattodea: Termitoidae) from Bogor, Indonesia Nadzirum Mubin; Giyanto Giyanto; Idham Sakti Harahap
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.191

Rayap merupakan serangga sosial yang berperan penting dalam perputaran siklus nutrisi. Di dalam sistem pencernaan rayap, terdapat simbion yang membantu proses degradasi selulosa. Penelitian ini bertujuan mengisolasi bakteri simbion yang terdapat di dalam saluran cerna rayap tanah. Penelitian diawali dengan koleksi rayap tanah di Kampus IPB University, diikuti isolasi bakteri simbion dari saluran cerna belakang (proktodeum) yang kemudian diidentifikasi berdasarkan morfologi, fisiologi, dan molekuler menggunakan gen 16S rRNA. Enam rayap tanah yang diperoleh adalah Macrotermes gilvus, Odontotermes javanicus, Microtermes insperatus, dan Capritermes mohri (Famili Termitidae); Schedorhinotermes javanicus dan Coptotermes curvignathus (Famili Rhinotermitidae). Dari enam rayap diperoleh 43 isolat dan satu isolat yang memiliki karakter umum. Isolat bakteri kode 8A_27F berwarna putih, elevasi seperti tombol dengan tepian licin. Dari uji fisiologis, isolat ini termasuk ke dalam bakteri Gram positif, berspora, dan bersifat aerob. Identifikasi dengan gen 16S rRNA menunjukkan bahwa isolat bakteri tersebut memiliki tingkat kemiripan sebesar 98% dengan spesies Bacillus endophyticus. Kata kunci: bakteri, rayap, 16S rRNA, simbion, proktodeum

Co-Authors Abdjad Asih Nawangsih Agus Eko Prasetyo Aisyah Imas ALI NURMANSYAH Andi Takdir Anwar, Aswaldi Aprizal Zainal Aris Budiman Awaludin Hipi Bambang Nuryanto Dadang Dadang DAMAYANTI BUCHORI Dori Kusuma Jaya DWI ANDREAS SANTOSA Efi Toding Tondok Elvina Efendi Eny Widajati Erina Sulistiani Gustan Pari Gustan Pari Haliatur Rahma, Haliatur Herliyana Indahwardani Idham Sakti Harahap Imas Aisyah Indahwardani, Herliyana Kikin H Mutaqin Kikin H. Mutaqien Kresnamurti Kurniasih Leiwakabessy, Christoffol Mahardika Gama Pradana Meity S Sinaga, Meity S Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Suharman, Memen Memen Surahman Mokhammad Danang Kusuma Mubin, Nadzirum Muhammad Evan Nurrahmawan Muhammad Yasin Sonhaji Novik Nurhidayat RAHAYU WIDYASTUTI Ratna Sari Dewi Sanjaya, Wilhelmus Terang Arga Sarah Desmia Muchtar Sarah Desmia Muchtar Sarijan, Abdullah - Sarjiya Antonius Satoshi Nakamura SATRIYAS ILYAS Seiichi Furukawa Setiawan, Asep S Sudarsono Supramana Syaiful Khoiri TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Tuminem ., Tuminem

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