Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

1.497

P-Index

This Author published in this journals

All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI)

. Giyanto

Department Of Plant Protection, Faculty Of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680

Author-ID : 209322

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Published : 27 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

POTENSI BAKTERI ENDOFIT DALAM MENEKAN PENYAKIT LAYU STEWART (PANTOEA STEWARTII SUBSP. STEWARTII) PADA TANAMAN JAGUNG Rahma, Haliatur; Zainal, Aprizal; Suharman, Memen; Sinaga, Meity S; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 14 No. 2 (2014): September, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Potential of endophytic bacteria to control stewart wilt disease (Pantoea stewartii subsp. stewartii) in maize. The purpose of this study was to explore endophytic bacteria from seedling, maize roots and grass roots as well as to test the ability of endophytic bacteria which could potentially suppress stewart wilt disease development in maize. Characterization of endophytic bacteria as biocontrol agents including: do not induce HR on tobacco, synthesize IAA, dissolve phosphate, produce siderophores, and antibiotic to Pantoea stewartii subsp. stewartii (Pnss). The results of research shoed 17 isolates of endophytic bacteria potentially as candidate biocontrol agents. Nine isolates were able to produce IAA, siderofores and phosphatase; two isolates produce IAA and phosphatase; six isolates produce IAA. Six isolates ie: AR1, AJ34, AJ15, AJ19, and AJ14 AN6, can increase maize plant resistance and suppress stewart wilt disease severity with a range of 48.95-55.60%.

KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO Mutaqin, Kikin Hamzah; ., Giyanto; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.

POTENSI BAKTERI ENDOFIT AKAR UBI JALAR (IPOMOEA BATATAS L.) ASAL KABUPATEN SORONG PAPUA BARAT SEBAGAI AGENSIA BIOKONTROL MELOIDOGYNE SPP. ., Tuminem; Sinaga, Meity S; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 15, No 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Potency of sweetpotato (Ipomoea batatas L.) root endophytic bacteria from Sorong District West Papua as biocontrol agent of Meloidogyne spp. Root knot nematodes/RKN, Meloidogyne spp. is one of the important pathogens in sweet potato plant. The disease incidence rate by the RKN on sweetpotato crop in Sorong District reached 88.77%. This study aims to get the sweet potato root endophytic bacteria that have potential as biocontrol agents against Meloidogyne spp. Endophytic bacteria was isolated from the roots of healthy sweet potato sampled from Sorong District, West Papua Province. Isolation and selection of bacteria using TSA media. Selected bacterial isolates, which were non-pathogenic to plants and humans then were identified with PCR technique using universal primer 63-F / 1387-R. The ability of bacteria to produce the lipase enzyme was selected using the media NB agar and rhodamine B. The protease enzyme-producing bacteria were selected using skim milk media. The chitinase enzyme-producing bacteria were selected using the colloidal chitin media. Production of cyanide was detected using filter paper soaked in a solution of CDS. The effectiveness of culture filtrate of bacteria as biocontrol agents was measured based on the percentage of 2nd juvenile mortality and egg hatching of Meloidogyne spp. Four isolates of endophytic bacteria, that were Enterobacter sp EAS (1a), Enterobacter sp. EAS (3a) Enterobacter ludwigii EAS (4), and Burkholderia cepacia EAS (6) produced lipase and protease. In addition, B. cepacia EAS (6) also produced chitinase. Those isolates caused mortality of the 2nd juvenile 81.4 to 95.2% and inhibited the egg hatching of Meloidogyne spp. 53.13 to 81.92%.

KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Sanjaya, Wilhelmus Terang Arga; Giyanto, .; Widyastuti, Rahayu; Santosa, Dwi Andreas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or ÃŽÂ²-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: ÃŽÂ²-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau ÃŽÂ²-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.

Uji Inokulasi dan Respon Ketahanan 38 Genotipe To mat terhadap Clavibacter michiganensis subsp. michiganensis Aprizal Zainal; Aswaldi Anwar; Satriyas Ilyas; , Sudarsono; , Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Identifi kasi ketahanan genotipe adalah langkah awal dalam pengembangan kultivar tahan terhadap serangan patogen. Tujuan penelitian ini adalah (i) mendapatkan cara inokulasi dengan jumlah dan konsentrasi inokulum Cmm yang efektif untuk mengevaluasi ketahanan tomat terhadap Cmm di rumah kaca, (ii) mendeterminasi reaksi ketahanan berbagai genotipe tomat akibat inokulasi Cmm. Percobaan ini menggunakan 38 genotipe tomat yang terdiri dari 7 genotipe tomat lokal, 15 genotipe tomat komersial, dan 16 genotipe koleksi Pusat Studi Pemuliaan Tanaman IPB Bogor (PSPT/IPB). Agen penyebab penyakit yang digunakan adalah 6 isolat Cmm hasil percobaan sebelumnya. Cara inokulasi Cmm yang efektif terhadap tomat cv. Marta (sangat rentan), uji reaksi ketahanan berbagai genotipe tomat terhadap Cmm telah dilakukan di rumah kaca. Kesimpulan dari penelitian ini adalah (i) inokulasi dengan menyuntikkan inokulum Cmm 5 µl konsentrasi 106 cfu/ml pada beberapa tempat di ketiak daun (daun pertama, daun tengah dan pucuk) merupakan cara yang paling efektif mengevaluasi ketahanan tomat terhadap Cmm, (ii) berbagai genotipe tomat yang diuji belum ada yang tahan teradap Cmm, genotipe tomat lokal ada yang agak rentan dan agak tahan.Kata kunci: kultivar tahan, suspensi bakteri, tomat

Perlakuan Benih untuk Meningkatkan Mutu dan Produksi Benih serta Mengendalikan Penyakit Bulai pada Jagung Manis Muhammad Yasin Sonhaji; Memen Surahman; Satriyas Ilyas; Giyanto .
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perlakuan benih untuk meningkatkan mutu dan produksi benihserta mengendalikan penyakit bulai pada jagung manis. Galur jagung manis 06 digunakan pada percobaan di rumah plastikyang disusun berdasarkan rancangan acak lengkap satu faktor, yaitu 13 perlakuan benih. Percobaan di lapangan disusunberdasarkan rancangan petak terbagi dengan dua faktor. Faktor pertama merupakan galur jagung yang terdiri atas duagalur yaitu galur 06 dan 07, dan faktor kedua merupakan perlakuan benih yang terdiri atas 7 perlakuan. Matriconditioningmenggunakan rasio benih : arang sekam : pelarut = 3:0.5:1 (g) dalam botol tertutup dan ditempatkan pada ruangan bersuhu20±2 C selama 24 jam. Fungisida sintetik yang digunakan mengandung metalaksil dan dimethomorf. Agen hayati yangdigunakan adalah Bacillus megaterium dan Brevibacillus laterosporus. Hasil percobaan di rumah plastik dan lapanganmenunjukkan bahwa perlakuan fungisida sintetik paling efektif menekan penyakit bulai. Percobaaan di lapangan menunjukkanbahwa galur 07 lebih tahan terhadap penyakit bulai dibanding galur 06. Perlakuan matriconditioning + fungisida sintetik+ B. laterosporus pada galur 07 menghasilkan bobot tongkol per tanaman paling tinggi. Perlakuan matriconditioning +fungisida sintetik + B. laterosporus pada galur 06 mampu meningkatkan mutu fisiologis benih hasil panen.Kata kunci: Bacillus megaterium, Brevibacillus laterosporus, matriconditioning, Peronosclerospora maydis

Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.) Anna Tefa; Eny Widajati; Muhamad Syukur; dan Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period

Uji Ketahanan Anggrek Hibrida Phalaenopsis terhadap Penyakit Busuk Lunak yang Disebabkan oleh Dickeya dadantii Refa Firgiyanto; Sandra Arifin Aziz; Dewi Sukma; dan Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTOne of the most popular orchids and cultivated by Indonesia and other countries is Phalaenopsis. The main disease of Phalaenopsis orchids in Indonesia is soft rot caused by bacteria Dickeya dadantii. The purpose of this study was to know the resistanctcy of Phalaenopsis hybrid to soft rot disease. The experiment was conducted at the Bacterial Plant Laboratory, Department of Plant Protection, the Greenhouse of Leuwikopo, Bogor Agricultural University, and the plastic house of Alam Sinar Sari Dramaga, Bogor from June 2014 to February 2015. The experimental design was randomized block design with three replications. Five genotypes of hybrid Phalaenopsis were tested, namely Phal. KHM 205, Phal. KHM 1126, Phal. KHM 1318, Phal. AMP 17, and Phal. KHM 2249. Phal. amabilis, Phal. esmeralda, Phal. amboinensis, and Phal. cornu-cervi were used as controls. The resistance testing was performed by inoculating bacteria D. dadantii on leaves of the orchids. The results showed all Phalaenopsis hybrid showed disease symptoms after inoculation. Phal. KHM 2249 had the lowest number of fallen leaves and the highest number of survive plants compared to the other hybrid Phalaenopsis. Survival rate was likely related to peroxidase activity and leaf thickness.Keywords: fallen leaves, leaf thickness, peroxidase, Phalaenopsis

Pelapisan Benih Menggunakan Bakteri Probiotik untuk Mempertahankan Viabilitas Benih Jagung Manis (Zea mays saccharata Sturt.) selama Penyimpanan Sarah Desmia Muchtar; Eny Widajati; . Giyanto
Buletin Agrohorti Vol. 1 No. 4 (2013): Oktober 2013
Publisher : Departemen Agronomi dan Hortikultura

Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pelapisan benih menggunakan bakteri Bacillus subtilis, Serratia marcescens, dan Pseudomonas kelompok fluorescens terhadap viabilitas dan daya simpan benih jagung manis (Zea mays saccharataSturt.). Penelitian disusun dengan rancangan petak tersarang (Nested Design) dengan petak utama adalah periode simpan minggu ke 0, 3, 6, 9, 12, 15, 18, 21, dan 24 dan anak petak adalah perlakuan coating dengan bakteri Bacillus subtilis, Serratia marcescens, Pseudomonas kelompok fluorescens, dan kontrol. Hasil penelitian menunjukkan adanya interaksi yang sangat nyata antara perlakuan coating benih dan periode simpan terhadap daya berkecambah, berat kering kecambah normal, kadar air, dan populasi bakteri. Perlakuan coating benih dan periode simpan menunjukkan interaksi yang nyata terhadap indeks vigor benih. Tolok ukur kecepatan tumbuh benihhanya dipengaruhi oleh faktor tunggal periode simpan. Benih yang dilapisi bakteri menghasilkan nilai berat kering kecambah normal yang nyata lebih baik daripada benih tanpa coating. Benih tanpa coating memiliki nilai daya berkecambah sebesar 56.7% pada periode simpan 24 minggu. Benih yang dilapisi bakteri Bacillus subtilis dapat mempertahankan daya berkecambah hingga 64.0% sampai periode simpan 24 minggu, sedangkan Serratia marcescens mampu mempertahankan daya berkecambah hingga 60.0% . Berdasarkan tolok ukur daya berkecambah, pelapisan benih menggunakan bakteri Bacillus subtilis dan Serratia marcescens merupakan perlakuan pelapisan benih yang potensial untuk lebih dikembangkan. Bakteri Bacillussubtilis lebih mampu bertahan hidup selama penyimpanan dibanding dengan bakteri Serratia marcescens maupun Pseudomonas fluorescens. Populasi bakteri Bacillus subtilis sampai dengan periode simpan 24 minggu adalah 14.2 × 104 cfu g-1.

Aplikasi Bakteri dalam Perlakuan Seed Coating untuk Mempertahankan Viabilitas dari Benih Cabai (Capsicum annuum L.) yang Sehat Herliyana Indahwardani; Eny Widajati; . Giyanto
Buletin Agrohorti Vol. 5 No. 1 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Penelitian ini bertujuan mengetahui pengaruh perlakuan pelapisan benih menggunakan bakteri Bacillus subtilis, Pseudomonas keompok. fluorescens dan Serratia marcescens terhadap viabilitas benih cabai (Capsicum annuum L.) selama di penyimpanan. Penelitian ini mengunakan rancangan petak tersarang dengan tiga ulangan. Petak utama adalah periode simpan (0, 3, 6, 9, 12, 15, 18, 21 dan 24 minggu) dan anak petak adalah perlakuan coating dengan bakteri tertentu (Bacillus subtilis, Pseduomonas kelompok fluorescens, Serratia marcescen dan kontrol). Benih cabai IPB C5 masih memiliki viabilitas yang cukup baik hingga akhir penyimpanan, ditunjukkan dengan nilai daya berkecambah sebesar 77.33%. Perlakuan kontrol dan coating menggunakan bakteri menunjukkan nilai daya berkecambah, indeks vigor, kecepatan tumbuh dan bobot kering kecambah normal yang tidak berbeda nyata. Ketiga bakteri yang digunakan sebagai pelapis masih dapat hidup sampai periode simpan 24 minggu dengan populasi 5.89 x 104 cfu g-1 untuk Pseudomonas kelompok fluorescens, 4.79 x 104 cfu g-1 untuk Bacillus subtilis dan 1.70 x 104 cfu g-1 untuk Serratia marcescens.

Co-Authors Abdjad Asih Nawangsih Agus Eko Prasetyo Aisyah Imas ALI NURMANSYAH Andi Takdir Anwar, Aswaldi Aprizal Zainal Aris Budiman Awaludin Hipi Bambang Nuryanto Dadang Dadang DAMAYANTI BUCHORI Dori Kusuma Jaya DWI ANDREAS SANTOSA Efi Toding Tondok Elvina Efendi Eny Widajati Erina Sulistiani Gustan Pari Gustan Pari Haliatur Rahma, Haliatur Herliyana Indahwardani Idham Sakti Harahap Imas Aisyah Indahwardani, Herliyana Kikin H Mutaqin Kikin H. Mutaqien Kresnamurti Kurniasih Leiwakabessy, Christoffol Mahardika Gama Pradana Meity S Sinaga, Meity S Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Suharman, Memen Memen Surahman Mokhammad Danang Kusuma Mubin, Nadzirum Muhamad Syukur Muhammad Evan Nurrahmawan Muhammad Yasin Sonhaji Novik Nurhidayat RAHAYU WIDYASTUTI Ratna Sari Dewi Refa Firgiyanto Sandra Arifin Aziz Sanjaya, Wilhelmus Terang Arga Sarah Desmia Muchtar Sarah Desmia Muchtar Sarijan, Abdullah - Sarjiya Antonius Satoshi Nakamura Satriyas Ilyas Seiichi Furukawa Setiawan, Asep S Sudarsono, Sudarsono Sukma, Dewi Supramana Syaiful Khoiri Tefa, Anna TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Tuminem ., Tuminem

Title

Found 27 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 27 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 27 Documents
Search