Vita Ratri Cahyani
Department of Soil Science, Faculty of Agriculture, Sebelas Maret University

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Kompatibilitas dan Efektivitas Azospirillum dan Streptomyces untuk Mengendalikan Penyakit Moler pada Bawang Merah di Alfisol Jumantono Hadiwiyono, Hadiwiyono; Fitriana, Faedah; Poromarto, Susilo Hambeg; Cahyani, Vita Ratri
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.50413

Abstract

Pengaruh Azospirillum sp. dan Streptomyces sp. secara terpisah sebagai perlakuan tunggal pada kegiatan budidaya tanaman telah banyak dilaporkan pada penelitian sebelumnya, tetapi sebagai perlakuan kombinasi sebagai agens hayati masih terbatas. Penelitian ini bertujuan untuk menganalisis kompatibilitas dan efektivitas Azospirillum sp. dan Streptomyces sp. untuk pengendalian penyakit moler yang disebabkan oleh Fusarium oxysporum f. sp. cepae (FOCe) dan pertumbuhan bawang merah di tanah Alfisol Jumantono. Penelitian terdiri dari uji in vitro (uji antagonisme dan uji kompatibilitas) dan uji in vivo (penanaman pada polybag di screen house). Uji in vitro dilakukan dengan metode dual culture sementara uji in vivo dilakukan dengan Rancangan Acak Kelompok Lengkap (RAKL) yang terdiri dari lima perlakuan dan lima ulangan. Perlakuan terdiri dari perlakuan tanpa agens pengendali hayati, aplikasi fungisida, Azospirillum sp., Streptomyces sp., dan kombinasi Azospirillum sp. dengan Streptomyces sp. semua perlakuan diinkulasikan dengan FOCe.Variabel yang diamati pada uji in vitro yaitu kompatibilitas dan penghambatan pertumbuhan koloni FOCe, sementara pada uji in vivo variabel yang diamati adalah penghambatan terhadap intensitase penyakit serta pengaruhnya terhadap pertumbuhan bawang merah. Hasil penelitian menunjukkan bahwa secara in vitro Azospirillum sp. dan Streptomyces sp. kompatibel satu sama lain. Streptomyces sp. menekan pertumbuhan FOCe sebesar 52.96% lebih baik daripada Azospirillum sp. Akan tetapi, hasil uji in vivo menunjukkan Azospirillum sp. dan Streptomyces sp., baik secara individu maupun bersama-sama, belum berhasil memberikan pengendalian yang optimal terhadap penyakit moler pada bawang merah yang ditanam di tanah Alfisols Jumantono. Meskipun aplikasinya mampu mengurangi intensitas penyakit moler, tingkat efektivitasnya termasuk dalam kategori kurang. Selain itu, dampaknya terhadap parameter pertumbuhan bawang merah juga belum hasil yang diharapkan. Oleh karena itu, diperlukan peningkatan dalam metode aplikasi agens pengendali hayati guna mencapai hasil yang diinginkan.
Exchangeable Al, peanut growth, and nodulation on Ultisol Bogor as affected by ameliorant proportion of lime and organic matter Cahyani, Vita Ratri; Firmansyah, Mohammad Iqbal; Cahyono, Ongko; Widijanto, Hery
Journal of Degraded and Mining Lands Management Vol. 11 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.112.5429

Abstract

Ultisols are characterized as acid soils with high exchangeable Al as the primary constraint for plant growth. This study aimed to investigate the effect of the proportion of lime and organic matter in decreasing exchangeable Al on Ultisol Bogor and continued to observe the impact on the growth and nodulation of peanuts. The pot experiment used a completely randomized design with a single treatment of the addition of ameliorant consisting of 12 levels of the treatment of lime (calcite and dolomite), organic matter (dry cow dung and rice straw compost), and their combinations with three replications. The first step was applying the ameliorant and incubating for eight weeks, then continuing the cultivation of peanuts. Results showed that during incubation, the decrease in exchangeable Al levels varied between treatments, along with an increase in soil pH and available P. The treatments of calcite 100% (T1) and dolomite 100% (T2) showed the highest increase in soil pH and available P and the highest decrease in exchangeable Al. The result of plant growth showed that the highest plant dry weight, N-uptake, and P-uptake were obtained by the treatment of dolomite 25% + rice straw compost 25% + dry cow dung 25% + NPK 25% (T10), whereas the highest number and total weight of nodules were obtained by the rice straw compost 50% + dry cow dung 50% (T7) and dolomite 25% + rice straw compost 75% (T8).  
Evaluation of Arbuscular Mycorrhizal Cultures in Increasing Phosphorus Uptake and Maize Growth Compared to Chemical and Organic Fertilizers on an Andisol Cahyani, Vita Ratri; Azzahra, Nadine Yuki; Rosariastuti, Retno
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.3867

Abstract

There is still limited information about the formulation of arbuscular mycorrhizal (AM) fungi culture with the specific ability to overcome P retention in Andisols. The purpose of this study is to evaluate the functional ability of eight AM fungi cultures consisting of four cultures from the generation I (A1I31, A2I21, A0I31, and A0I21) and four cultures from generation II (A1I32, A2I22, A0I32, and A0I22) in dealing with P constraints on an Andisol, compared with the application of fresh AM fungi inocula isolated from natural soils, synthetic chemical fertilizers (CF), rice straw (RS) compost, and several combination treatments including Bio-RP Nutrition. The highest functional ability in increasing P uptake and maize growth on Andisol is obtained by A1I32, followed by A2I22 and A0I22, indicating that AM fungi cultures generation II exhibited higher effectiveness than generation I. The increase of P uptake and maize shoot dry weight yielded by those three AM fungi cultures were in the range of 80-97% and 89-103% of T14 (CF 100%), indicating the high potential biofertilizers for reducing the use of chemical fertilizers. By  cultivation  plate  method,  the  present  findings  also  confirmed that  AM   fungi   inoculation   affecting  significantly   the   abundance and  the  composition  of  foliar  endophytic  bacterial  communities.
Dynamic capability of P-solubilizing endophytic and rhizospheric bacteria from various vegetation of Alas Bromo for reducing chemical fertilizer use Prasgi, Henokh Christian; Ariyanto, Dwi Priyo; Cahyani, Vita Ratri
Journal of Degraded and Mining Lands Management Vol. 12 No. 3 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.123.7617

Abstract

The application of agrochemicals is still the main practice in the agroforestry system of Alas Bromo. Alfisols in Alas Bromo were characterized as acidic soil with very low soil available P. This study aimed to examine the capacity of P-solubilizing endophytic and rhizospheric bacteria from various Alas Bromo vegetation: mahogany, wresah, peanut, elephant grass, and maize in increasing soil available P and enhancing maize growth, which has the potential for reducing chemical fertilizer use. The research began with exploring and isolating bacteria using Pikovskaya agar medium, followed by in vitro tests of functional abilities and greenhouse experiments (soil incubation continued with maize planting). Rock phosphate was used as a P-inorganic source with a dose of 150 kg   ha-1. Based on the highest PSI, a total of 15 out of 52 isolated bacteria from 5 vegetations were selected to be tested in vitro and in a greenhouse experiment. Among the assessed bacteria, 3 endophytic bacterial isolates of Ad_R_5, Ah_R_4, and Zm_L_6 showed consistently high performance during successive assessments. Compared to basal treatment, the increases of soil available P, shoot fresh weight, and shoot dry weight by these 3 superior isolates ranged from 51.11-90.29%, 38.27-89.75%, and 57.04-88.73%, while the increases by NPK were 93.53, 113.67 and 107.04%, indicating the isolates had high potential for reducing chemical fertilizer use. The 3 superior isolates were identified as Pantoea dispersa, Ralstonia picketii, and Enterobacter hormaechei, respectively. Further study is needed to investigate the other potential capabilities, such as pesticide degradation and supporting plant resistance to drought stress.
Rice growth and the associated phosphate solubilizing bacteria on an Alfisol treated with insecticide and endophytes inoculum Shofiyah, Laily; Sudadi, Sudadi; Cahyani, Vita Ratri
Journal of Degraded and Mining Lands Management Vol. 12 No. 5 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.125.8481

Abstract

The long-term use of excessive amounts of chemical fertilizers and pesticides adversely affects soil fertility, crop productivity, and biodiversity of soil and endophytes microbes. This study evaluated the ability of endophytic bacteria isolated from rice plants to enhance soil fertility, promote plant growth, and induce rice plant resistance on Alfisols treated with insecticide 10x usage dose (chlorantraniliprole 100 g L-1 and thiamethoxam 200 g L-1), and also assessed the diversity and population density of the associated phosphate solubilizing rhizospheric and endophytic bacteria. Three isolates of Burkholderia sp. YErOI-1 (I1), Bacillus sp. NErOI-2 (I2), Burkholderia sp. PElOI-3 (I3) and a mixture of three isolates (I4) were tested in combination with (P1) and without (P0) insecticide treatments in the pot cultivation of rice Inpari 32 using Alfisols in a completely randomized factorial design with three replications. The results showed that the control (I0P0) demonstrated higher plant growth compared to I0P1, indicated insecticide treatment resulted in toxicity effect. Among all the treatments, I2P0, I2P1, and I1P0 yielded the highest plant growth, whereas the lowest was indicated by I1P1. The rice associated phosphate solubilizing rhizospheric and endophytic bacteria indicated the highest population density and diversity index on the treatments I3P0 and I3P1 which significantly correlated with the highest P concentration and P uptake in these two treatments. Isolate I2 showed the strongest effect in inducing plant growth to insecticide resistance, in contrast isolate I1 showed no support in inducing plant resistance to insecticide. Further study is needed to examine the effect of the present assessed endophytes in other type of pesticide treatments.
Effect of biological agents on chlorpyrifos content in soil and bulbs, soil fertility, and shallot (Allium ascalonicum L.) yield Saputri, Agustin Ayu; Rahayu, Rahayu; Widijanto, Hery; Cahyani, Vita Ratri; Rosariastuti, Retno
Journal of Degraded and Mining Lands Management Vol. 12 No. 5 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.125.8735

Abstract

Continuous pesticide application in shallot (Allium ascalonicum L.) cultivation can reduce soil chemical and biological fertility, potentially leading to the accumulation of pesticide residues. Sustainable agricultural practices are therefore needed to restore soil fertility and improve shallot yield. This study was conducted as a field experiment using a randomized complete block design with a single-factor treatment, namely the type of biological agent: control (BA0), Atlantibacter hermannii (BA1), Pseudomonas sp. (BA2), Indigenous bacterial consortium (BA3), Eco enzyme (BA4), and Biofilm (BA5) from Mount Lawu's western slopes. Each experimental treatment was repeated five times, resulting in a total of 30 units. Statistical analysis was done using analysis of variance (ANOVA), followed by Duncan multiple range test (DMRT) and Pearson correlation. The parameters observed included chlorpyrifos levels, chemical and biological characteristics, and shallot yield. The results of the study indicate that Atlantibacter hermannii (BA1) reduced soil chlorpyrifos content by 64.62% and increased shallot yield by 41.44%. Eco enzyme (BA4) and biofilm (BA5) were the most effective in lowering chlorpyrifos accumulation in bulbs, with reductions of 42.31% and 38.46%, respectively. Regarding soil fertility, BA1 increased available phosphorus by 30.32%, Pseudomonas sp. (BA2) increased available potassium by 12%, and BA4 increased total nitrogen by 18.18%; however, the improvements from BA2 and BA4 were similar to those from BA1. Overall, Atlantibacter hermannii (BA1) and eco enzyme (BA4) emerged as the most promising biological agents for reducing pesticide residues, enhancing soil fertility, and increasing shallot production, highlighting their potential application in sustainable agriculture.
Effects of soil amendment from herbal and eucalyptus industrial waste on methane emission and rice yield Kurniawati, Feriana Dwi; Suntoro, Suntoro; Setyanto, Prihasto; Cahyani, Vita Ratri
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 2 (2023): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i2.69297

Abstract

The use of chemical fertilizer in rice fields contributes to increased global warming via enhanced emission of methane (CH4) into the atmosphere. Therefore, composting has been proposed to reduce methane emissions in the agricultural field. This study aimed to determine the CH4 emission and rice yield affected by compost from three different types of compost: herbal compost, eucalyptus compost, and manure compost. This randomized block design study was conducted from November 2019 to May 2020. There were 8 fertilizer treatments applied to the rice fields, namely: herbal compost 10 tons/ha. (O1), eucalyptus compost 10 tons/ha (O2), manure compost 10 tons/ha (O3), no compost no chemical fertilizer (as a control) (O4), herbal compost 5 tons/ha + chemical fertilizer/CF (C1), eucalyptus compost 5 tons/ha + CF (C2), manure compost 5 tons/ha + CF (C3), and only chemical fertilizer (C4), then all treatments replicated three times. For the chemical fertilizer (CF) the dose is 166 kg/ha urea + 166 kg/ha ZA + 330 kg/ha TSP. The result indicated that the compost manure 10 tons/ha (O3) and the combination compost manure 5 tons/ha + CF (C3) produced the highest rice yields (6.89 -6.94 tons/ha) but impacted the highest methane emissions (505.3 – 544.6 Kg.CH4 /ha/season). The important finding showed that among all the treatments, a combination of compost eucalyptus 5 tons/ha + CF (C2) and compost eucalyptus 10 tons/ha (O2) mitigated methane emission to the lowest level (296.6 -305.2 Kg.CH4/ha/season) and gave high rice yields (6.77-6.78 tons/ha) that were not significantly different from those of compost manure (O3 and C3). In addition, the combination of compost herbal 5 tons/ha and chemical fertilizer (C1) affected the lower methane emissions than manure compost and gave a high level of grain yield that was not significantly different from those of manure compost (O3 and C3) and eucalyptus compost (O2 and C2). 
Soil Fertility Status, Nutrient Uptake, And Soybean Yield Following OrganicPLUS Fertilizer Application On Alfisol Minardi, Slamet; Sudadi, Sudadi; Cahyono, Ongko; Cahyani, Vita Ratri; Maro'ah, Siti; Haniati, Isna Luthfa; Wijaya, Lidya Zaela
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.82987

Abstract

This study aims to assess the impact of Organic FertilizerPlus on soil fertility status, nutrient uptake, and soybean yield in Alfisol. The research employs a randomized complete group design (RCGD) with a single factor, consisting of P0 (Control), P1 (Cow dung, 5 tons ha-1 + Zeolite dose of 2.5 tons ha-1), P2 (Cow dung, 5 tons ha-1 + Zeolite dose of 5 tons ha-1), P3 (Cow dung, 5 tons ha-1 + Dolomite dose of 2.5 tons ha-1), P4 (Cow dung, 5 tons ha-1 + Dolomite dose of 5 tons ha-1), and P5 (Cow dung, 5 tons ha-1 + Zeolite dose of 5 tons ha-1 + Dolomite dose of 5 tons ha-1), repeated four times. The results indicate that the application of Organic FertilizerPlus enhances soil fertility status (pH, organic C, CEC, and available P), with the highest values observed in treatment P5, including pH (6.70), soil organic C (6.28 g.kg-1), cation exchange capacity (CEC) (6.85 cmol.g-1), and available P (2.63 mg.g-1). This leads to increased phosphorus uptake, consequently improving soybean yields. The highest soybean yield is achieved by applying Organic FertilizerPlus (P5) at 2.17 t.ha-1.
Composting of Rice Straw–Based Materials using Aerobic Bioactivator Isolated from Rice Straw, Mahogany Bark and Cassava Peels Cahyani, Vita Ratri; Rahayu, Rahayu; Lakshitarsari, Kynthavi Paramitha; Megow, Rahma Amira Zhalzhabila Wakak; Azzahra, Nadine Yuki
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 1 (2024): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i1.74297

Abstract

Compost is one of organic fertilizers that play an important role in maintaining soil health and supporting sustainable agriculture. Diverse aspects could be developed to increase the quality of compost. This study aims to compare the quality of compost produced by using two different bioactivators, namely aerobic bioactivator RMC (the microbial consortia isolated from composted rice straw, mahogany bark and cassava peels + additional supplement) and fermentative bioactivator (EM4 + molasses) in the composting of the mainly rice straw-based materials. Composting was conducted using a single factor completely randomized design consisting of five levels as follows: C0 (control, rice straw); C1 (rice straw + bioactivator EM4 + molasses); C2 (rice straw + bioactivator RMC + additional supplement); C3 (rice straw + cow dung + bioactivator RMC + additional supplement); C4 (leaf litter + cow dung + bioactivator RMC + additional supplement, as comparison treatment with no rice straw). Among the treatments of C0, C1 and C2, composting the same rice straw material but different bioactivators, C2 showed the highest compost quality and decomposition rate. Among the other three treatments of C2, C3 and C4 composting different materials but using the same bioactivator, C3 showed the highest compost quality, but the three treatments showed the same high decomposition rate. Based on the characteristics of the compost product, including nutrient content, the treatment C3 produced the highest quality, followed by C4 and then C2. Both bioactivators EM4 + molasses and RMC+ additional supplement tended to increase total bacteria, fungi, nitrogen-fixing bacteria and phosphate-solubilizing microbiota in the compost products compared to the control without bioactivator. A set of aerobic bioactivator RMC plus additional supplement serve as one strategy to accelerate the composting process and to enhance the compost quality.
Penyuluhan dan Praktik Budidaya Tayurdapot untuk Kelompok Tani “Abdi Dalem Klebengan”, Kelurahan Kadipiro, Banjarsari, Surakarta Sudadi, Sudadi; Minardi, Slamet; Cahyono, Ongko; Cahyani, Vita Ratri; Maro'ah, Siti
PRIMA: Journal of Community Empowering and Services Vol 8, No 2 (2024): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v8i2.66853

Abstract

Counseling and Practices of Tayurdapot Cultivation for Farmers Group “Abdi Dalem Klebengan”, Kadipiro Village, Banjarsari, Surakarta. Citizens association (RW) 17 as a partner in this community service activity is one of the RWs in Kelurahan Kadipiro, Banjarsari District, Surakarta City which has a farmer group, namely the Farmer Group (Poktan) "Abdi Dalem Klebengan" which until now has been very active in empowering the community in agriculture. Even though it is located in the city area, Poktan "Abdi Dalem Klebengan" realizes that agricultural activities are one of the sectors that can help maintain the community's economy. Therefore, Poktan "Abdi Dalem Klebengan" is very active in trying to make the people in their area, namely RT 01 RW 17, work on agriculture around their settlements, including in the yards of their respective houses by working on potted plants to help the family's economy. One type of plant that is easy to cultivate in a pot is a vegetable plant (Plant vegetables in a Pot-Tayurdapot). This service activity aims to awaken and maintain the spirit of farming around the house for members of the Poktan "Abdi Dalem Klebengan" in particular and the community of RT 01 RW 17 Ngipang, Kadipiro, Banjarsari, Surakarta in general. Activities are carried out by the method of socialization activities and demonstrations of Tayurdapot cultivation practices in synergy with the Poktan "Abdi Dalem Klebengan". Through this effort, it is hoped that the families of the group members and the community of RT 01 RW 17 will be helped by their need for vegetables that can reduce daily shopping costs. Poktan "Abdi Dalem Klebengan" also produces organic fertilizer from its livestock business so that it can synergize with this service activity through its help organic fertilizer as a planting medium for tayurdapot for residents of RT 01 RW 17. This service activity is mono-year, but is expected to continue in the following year through other productive activities such as tabulampot, livestock business, fish rearing and guidance on the production of organic fertilizers and liquid biofertilizers.