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All Journal Jurnal Teknologi Dan Industri Pangan Hemera Zoa Jurnal Ilmu Ternak Veteriner
D Sastradipradja
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Industrial & Manufacturing Engineering Veterinary

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Anthiypercholesterolaemic Activity of Zincophyllin . Alsuhendra; D Muchtadi; D Sastradipradja; T Wresdiyati
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Pheophytin was obtained from chlorophyll that was extracted from fresh cassava leaves by use of ethanol. One hundred to 200 ppm of zinc was added to pheophytin to produce a complex of zinc-pheophytin (“zincophyllin”). The antihypercholesterolaemic effect of “zincophyllin” was studied in 20 male New Zealand White rabbits fed on a low-cholesterol (0.1%) diet for 12 weeks. Five animals were given 33.4 mg/d (doses I), 5 animals received 100.2 mg/d (doses II) of “zincophyllin”, 5 animals received standard diet as negative controls, and the remaining 5 animals served as cholesterol-fed controls (positive controls). Animal receiving dose Ii had significantly lower serum total and LDL-cholesterol concentrations. Positive control group and doses I group had significantly higher serum total cholesterol. Cholesterol concentration of group II slightly increased for 4 weeks, but decreased to normal until 12 weeks. Triglyceride concentration of all rabbits groups were in normal level, but LDL-cholesterol concentration of positive control group and doses I group were higher than others. It was suggested that the antihypercholesterolaemic effect of “zincophyllin” may benefit rabbits by inhibiting the increase of serum cholesterol concentration. Key words: Chlorophyll, pheophytin, “zincophyllin”, hypercholesterolaemic
Uji radioimun untuk progesteron G Wanananda; D Sastradipradja; P Paridjo
Hemera Zoa Vol. 71 No. 1 (1983): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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A radioimmunoassay (RIA) for progesterone plasma of various animal species had been applied and validated in our laboratory. The method used antisera against 11 alpha hydroxy progesterone raised in goats (donated by Dr. L.E Edqvist, Sweden) in a 1:2000 dilution. A sensitivity of 10 pg/ml was obtained based on the readings for this concentration which was statistically different from a zeroconcentration (p
Profil hormon progesteron dan luteinizing hormone (LH) kerbau betina dalam keadaan reproduksi normal dan setelah pemberian PGF2 Alpha G Wanananda; D Sastradipradja; P Paridjo; R Widjajakusuma; H Permadi; Iskandar .; A Soetisna; J.T. Batussama
Hemera Zoa Vol. 71 No. 1 (1983): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Dalam penelitian ini dilakukan tehnik radioimmunoassay (RIA) untuk analisa hormon progesteron dan LH pada kerbau betina bersiklus normal dan setelah pemberian PGF2 alpha. Hewan percobaan yang dipakai adalah delapan hewan krbau betina dewasa yang diberi makan dan minum secara ad libitum. Prepara PGF2 alpha yang diberikan adalah Enzaprost-F, secara intra-uterin, sebanyak dua kali pemberian dengan selang waktu sebelas hari dengan dosis 3-7 mg/hewan setiap kali pemberian. Pada profil hormon normal kadar progesteron mulai meningkat pada hari ke-6 dan mencapai puncak pada hari ke-12 yaitu 423 +- 60.3 pg/ml. Kadar progesteron pada saat estrus berkisar antara 24-253 pg/ml. Kadar LH basal adalah sekitar 0.1 pg/ml. Kadar progesteron setelah penyuntikan PGF2 alpha pertama maupun kedua berkisar antara 28-200 pg/ml. Profil LH setelah perlakuan pada dasarnya sama dengan yang diamati untuk siklus normal. Kerbau betina bersiklus normal memperlihatkan pola hormon progesteron dan LH dalam kualitas tidak berbeda dengan pola pada hewan ternak pemamah biak lain. Perbedaan itu terletak pada nilai kadar hormon yang lebih rendah yang dapat disebabkan oleh berbagai faktor seperti misalnya ciri genetik bangsa hewan dan mungkin juga berbagai keadaan cekaman (stress) seperti kualitas dan kuantitas makanan yang kurang baik, kondisi eksperimen, tatalaksana yang kurang baik dan sebagainya. Perlakuan dengan PGF2 alpha menyebabkan regresi corpus luteum yang dicerminkan oleh penurunan kadar peogeteron sampai ke nilai basal. Tumbuhnya puncak LH merupakan indikasi terjadinya ovulasi. Berdasarkan data tersebut, PGF2 alpha dapat dipakai sebagai penyerentak birahi pada kerbau yang diikuti oleh kemungkinan fertilitas, jika didukung oleh proses pematangan ovum yang cukup pada saat pemberian PGF2 alpha yang kedua.
Strategic study on energy-protein requirements for local sheep: 4. Ewes during late pregnancy I-W Mathius; D Sastradipradja; T Sutardi; A Natasasmita; L.A Sofyan; D.T.H Sihombing
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 3 (2002): SEPTEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.288 KB) | DOI: 10.14334/jitv.v7i3.291

Abstract

Thirty-six Javanese thin-tail ewes were set out to study the energy and crude protein requirements for the last eight-week of pregnancy. The ewes were randomlly assigned to a 3 x 3 factorial arrangement and offered nine diets comprising three levels of energy (low, medium and high) and three levels of crude protein (low, medium and high) with four ewes per treatment. The diets were pelleted and offered four times daily at approximately in the same amount. Results showed that, despite a difference of energy concentration in the diets, dry matter intake was not significantly (P>0.05) affected, but crude protein concentration was (P<0.05). Energy concentration in the diet also had no effect (P>0.05) on protein intake. However, protein concentration in the diets significantly affected on protein intake and protein excreted in the feces. Moreover, an interaction between energy and protein levels was not detected on energy and nitrogen availability utilization. This study demonstrated that a high efficiency on nitrogen utilization could be achieved on low crude protein diets. It was also found that crude protein and energy requirements for maintanance during pregnancy phase were 0.67 g/kg BW0.75 and 0.425 MJ EM/kg BW0.75 respectively.   Key words: Protein-energy requirement, pregnancy phase, ewes  
Strategic study on energy-protein requirements for local sheep: 5. Ewes during lactation phase I-W Mathius; D Sastradipradja; T Sutardi; A Natasasmita; L.A Sofyan; D.T.H Sihombing
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 1 (2003): MARCH 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.531 KB) | DOI: 10.14334/jitv.v8i1.370

Abstract

Thirty-six Javanese thin-tail ewes in the end of late pregnancy phase were set out to study the energy and crude protein requirements during the first eight-week of lactation phase. The ewes were penned individually in doors and randomly assigned to a 3 x 3 factorial arrangement, consisting of three levels of energy (low, medium and high) and three levels of crude protein (low, medium and high) diets with four ewes per treatment. The diets were pelleted and offered four times daily in approximately equal amount. Feed intake, nutrient digestibility, body weight and milk production were recorded. Results showed that, total lamb birth weights was not affected, but protein content on the ration treatments significantly altered (P<0.05) milk yield and composition. Dry matter intake due to feeding treatments was not significantly affected (P>0.05), while crude protein content on the ration highly significantly affected (P<0.01). Based on data recorded, the energy and protein requirements for ewes during lactation phase are highly significantly depended on ewes’ live weight, milk production and the ratio of energy metabolism and crude protein of the ration. It was concluded that in order to fulfil the crude protein and energy needs of the ewes during lactation phase, the ration given should contain crude protein and energy as much as 16% (based on dry matter) and 13.4 MJ/kg dry matter respectively.   Key words: Energy-protein requirement, lactation phase, local ewes  
Co-Authors A Natasasmita A Soetisna Alsuhendra Alsuhendra D Muchtadi D.T.H Sihombing G Wanananda H Permadi I-W Mathius Iskandar . J.T. Batussama L.A Sofyan P Paridjo R Widjajakusuma T Sutardi T Wresdiyati