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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu Ternak ANNALES BOGORIENSES Annales Bogorienses
Hartati Chairunnisa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Putranto, Wendry Setiyadi; Suradi, Kusmajadi; Chairunnisa, Hartati; Mustopa, Apon Zaenal; Kusumaningrum, Harsi Dewantari; Suhartono, Maggy Thenawidjaja
Annales Bogorienses Vol. 21 No. 1 (2017): Annales Bogorienses
Publisher : BRIN

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Abstract

Rennin-like protease of many microorganisms behave similarly to chymosin and are potential alternatives of rennet. The lactic acid bacteria with milk clotting activity were isolated from Bakasam, an Indonesian traditional fermented meat. Screening assay was carried out using modified method of skim milk agar and milk clotting activity test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/mL. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% identical with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.
Co-Authors Andry Pratama Anita Fitriani Apon Zaenal Mustopa Apon Zaenal Mustopa Denny Suryanto Sutardjo Giriwono , Puspo Edi Harsi D. Kusumaningrum Hendronoto AW. Lengkey Jajang Gumilar Kusmajadi Suradi Kusmajadi Suradi Lilis Suryaningsih Maggy Thenawidjaja Suhartono Nina Mardiana (F01108057) Obin Rachmawan Roostita L Balia Stefanus Jemianus Lepa Trianing Tyas Wendry Setiadi Putranto Wendry Setiyadi Putranto