Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

2.042

P-Index

This Author published in this journals

All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Veteriner Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar Jurnal Penelitian Pascapanen Pertanian Jurnal Pangan dan Agroindustri agriTECH BERITA BIOLOGI ANNALES BOGORIENSES TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Jurnal Kedokteran Hewan Jurnal Standardisasi

Harsi D. Kusumaningrum

Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB, Darmaga, Jl. Lingkar Akademik, Babakan, Kec. Dramaga, Bogor, Jawa Barat 16680

Author-ID : 303157

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

Published : 37 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).

Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Putranto, Wendry Setiyadi; Suradi, Kusmajadi; Chairunnisa, Hartati; Mustopa, Apon Zaenal; Giriwono, Puspo Edi; Kusumaningrum, Harsi Dewantari; Suhartono, Maggy Thenawidjaja
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.Â Â

Partial Sequencing of 16S rRNA Gene of Selected Staphylococcus aureus Isolates and its Antibiotic Resistance Harsi Dewantari Kusumaningrum; Lita Handayani; Reni Nofrianti
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The choice of primer used in 16S rRNA sequencing for identification of Staphylococcus species found in food is important. This study aimed to characterize Staphylococcus aureus isolates by partial sequencing based on 16S rRNA gene employing primers 16sF, 63F or 1387R. The isolates were isolated from milk, egg dishes and chicken dishes and selected based on the presence of sea gene that responsible for formation of enterotoxin-A. Antibiotic susceptibility of the isolates towards six antibiotics was also tested. The use of 16sF resulted generally in higher identity percentage and query coverage compared to the sequencing by 63F or 1387R. BLAST results of all isolates, sequenced by 16sF, showed 99% homology to complete genome of four S. aureus strains, with different characteristics on enterotoxin production and antibiotic resistance. Considering that all isolates were carrying sea gene, indicated by the occurence of 120 bp amplicon after PCR amplification using primer SEA1/SEA2, the isolates were most in agreeing to S. aureus subsp. aureus ST288. This study indicated that 4 out of 8 selected isolates were resistant towards streptomycin. The 16S rRNA gene sequencing using 16sF is useful for identification of S. aureus. However, additional analysis such as PCR employing specific gene target, should give a valuable supplementary information, when specific characteristic is expected.

FERMENTASI SUFU RENDAH GARAM DENGAN MENGGUNAKAN BEBERAPA KAPANG INDIGENUS DAN LACTOBACILLUS PLANTARUM KIK [Fermentation of Low Salt Sufu using Indigenous Moulds and Lactobacillus plantarum kik] Nurhayati Nurhayati; Betty S. L. Jenie; Harsi D. Kusumaningrum
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.837 KB)

Sufu is a traditional Chinese fermented soybean curd (tofu) resembling a soft creamy cheese-type product. It is made by fungal solid-state fermentation of tofu (called pizi) followed by aging in saturated brine solution. The aims of this study were to obtain the best indigenous mold strain for sufu fermentation and produce a low salt sufu by applying Lactobacillus plantarum kik. Four indigenous mold strains were used i.e Rhizopus oligosporus, R. oryzae, Mucor hiemalis and Actinomucor elegans during pizi fermentation. The salt concentrations used in brine fermentation varied in the range of 6% - 12%. The results showed that the fermentation time of pizi depended on the mold species. Based on the density of the mycelium and the spores colour, pizi fermented by R. oligosporus and R. oryzae were produced after 24 hours of fermentation, while those with M. hiemalis and A. elegans were formed after 36 hours at room temperature and 55-68% relative humidity (RH). Sensory evaluation of the pizi flavor indicated that the pizi fermented by A. elegans and R. oligosporus were ranked as first and second, respectively. Sensory evaluation (Balance Incomplete Block Rating Design) on the hedonic rating of sufu revealed that fermentation in 9% brine by Lactobacillus plantarum kik produced the most preferred sufu according to the panelists. Combination of L. plantarum kik and pasteurization of sufu could maintain the quality for three weeks.

CEMARAN ASPERGILLUS FLAVUS DAN AFLATOKSIN PADA RANTAI DISTRIBUSI PRODUK PANGAN BERBASIS JAGUNG DAN FAKTOR YANG MEMPENGARUHINYA [Contamination of Aspergillus flavus and Aflatoxin at Distribution Chain of Maize Based Food Product and its Influencing Factors Harsi D. Kusumaningrum; . Suliantari; Aris D. Toha; Sindhu H. Putra; Aldilla S. Utami
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 2 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.597 KB)

Aflatoxin is a human carcinogen, produced by fungus Aspergillus flavus that frequently contaminates maize. Analysis of A. flavus by plate counting and aflatoxin by Thin Layer Chromatography were performed on 104 samples and 25 samples, respectively, of maize grain and maize based food products from different places in Bogor-West Java, Boyolali-Central Java and Bojonegoro-East Java. These regions support significant number of maize production in Java. Forty percent of the samples were contaminated by A. flavus, whereas aflatoxin level of higher than 20 ppb was found in 4 of 25 samples. The highest contamination level of A. flavus was found at the collector trader that often stored the maize grain in average of 15 days, at room temperature. There was a significant correlation between the length of storage as well as relative humidity with the contamination levels of A. flavus. Significant correlation was also found between the contamination levels of A. flavus with the level of aflatoxin in maize grain. However, no significant correlation was found between the aflatoxin level and the contamination levels of A. flavus in the processed maize based food products.

AKTIVITAS ANTIBAKTERI EKSTRAK TUMBUHAN LUMUT HATI (Marchantia paleacea) TERHADAP BAKTERI PATOGEN DAN PERUSAK PANGAN [Antibacterial Activity of Liverwort (Marchantia paleacea) Extract on Pathogenic and Food Spoilage Bacteria] Reza Fadhilla; Eka Aditya Iskandar; Harsi Dewantari Kusumaningrum
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Liverwort (Marchantia paleacea) from Cibodas Botanical Garden Bogor Indonesia was used as raw materials in this research. The objective of this research was to assess the potential of antibacterial activity of liverwort (M. paleacea). Maceration using 4 different solvents i.e ethanol, methanol, ethyl acetate, and hexane was used to extract the active compound. The potential of antibacterial activity of the extracts was assesed by agar diffusion method and direct-contact test by determining the minimum inhibitory concentration (MIC). The data showed that the ethanol extract had the biggest inhibition zone on three test bacteria. The inhibition zone and MIC were 10.8 mm and 0.7 mg/ml for S. aureus; 4.5 mm and 8.0 mg/ml for S. Typhimurium; 5.8 mm and 5.9 mg/ml for P. aeruginosa, respectively. The total phenol content of the extract was 22 mg/g. Phenolic, triterpenoid and flavonoid were positively detected in the extracts.

QUANTIFICATION OF Salmonella Typhimurium REDUCTION DURING COLD STORAGE OF RAW SHRIMPS IN THE PRESENCE OF SODIUM METABISULFITE [Kuantifikasi Reduksi Salmonella Typhimurium pada Udang Segar selama Penyimpanan Dingin dengan Penambahan Natrium Metabisulfit] Harsi Dewantari Kusumaningrum; Andiarto Yanuardi
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Prediction of bacterial growth, survival or reduction in food matrices is needed for microbiological risk assessment. The survival of Salmonella Typhimurium on surfaces of raw shrimps at low temperature was studied, in the presence of sodium metabisulfite which is often used to prevent melanosis. The growth and/or reduction rates were quantified using DMFit software with Baranyi model and or linear model. The result showed that without sodium metabisulfite (control), when the initial level was high (105 CFU/ml), S. Typhimurium grew with a lag phase of 51.99±7.46 h and a growth rate of 0.01±0.002 log CFU.ml-1.h-1 on raw shrimps during storage at 8±2°C. When 1.5% (w/w) sodium metabisulfite, a maximum level that often used to prevent melanosis, was added under the same condition, the number of S. Typhimurium was reduced for 5 log CFU/ml after 5 days, with a reduction rate of -0.03±0.001 log CFU.ml-1.h-1. This study indicated that Baranyi model can be used to predict the growth of S. Typhimurium on raw shrimp at low temperature, when sodium metabisulfite is absent. However, when sodium metabisulfite is present, at least 0.4% as found in this study, the reduction of S. Typhimurium can be predicted using a simple linear model.

ISOLASI Campylobacter DARI KARKAS AYAM MENGGUNAKAN METODE KONVENSIONAL DAN POLYMERASE CHAIN REACTIONS [Isolation of Campylobacter from Poultry Carcasses using Conventional and Polymerase Chain Reaction Methods] . Andriani; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

ABSTRACT Campylobacter jejuni and Campylobacter coli are two spesies of Campylobacter sp. frequently found as pathogenic bacteria causing human gastrointestinal infections. Contaminated chicken carcasses have been reported as the source of human campylobacteriosis. In this study, Campylobacter were isolated from chicken carcasses sold in traditional markets and supermarkets. In traditional markets, chicken carcasses are sold without proper packaging or in an open space and stored at room temperature (25-30°C) for prolonged period allowing pathogenic bacteria to grow. While at supermarkets, chicken carcasses are openly displayed or enclosed in plastic wrappings and stored in a refrigerator (4-8°C). A total of 298 samples of chicken carcasses from traditional markets and supermarkets in the area of DKI Jakarta, West Java (Bogor and Sukabumi) and Central Java (Kudus and Demak) were collected. Isolation and identification using conventional and Polymerase Chain Reactions (PCR) methods were done to determine the prevalence of C. jejuni and C. coli contamination in poultry. The results showed that chicken carcasses sold in the sampling area, both traditional markets and supermarkets, were contaminated with C. jejuni and C. coli. The contamination rate of Campylobacter sp. in chicken carcasses sold in supermarkets, were 14.1% by conventional methods and 29.5% by PCR. This was higher than those in traditional markets, i.e. 5.7 and 12.1%, respectively. It is also confirmed that the prevalence for contamination of C. jejuni was higher than C. coli in 298 samples, i.e. 16.1% and 3.7% by conventional method and 23.5% and 18.1% by PCR method respectively. Keywords: Campylobacter coli, Campylobacter jejuni, poultry carcasses, supermarket, traditional market

AKTIVITAS ANTIBAKTERI GETAH PEPAYA KERING TERHADAP Staphylococcus aureus PADA DANGKE [Antibacterial Activity of Dried Papaya Latex toward Staphylococcus aureus in Dangke] Rifah Hestyani Arum; Budiatman Satiawihardja; Harsi D. Kusumaningrum
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

AKTIVITAS ANTIBAKTERI GETAH PEPAYA KERING TERHADAP Staphylococcus aureus PADA DANGKE[Antibacterial Activity of Dried Papaya Latex toward Staphylococcus aureus in Dangke]Rifah Hestyani Arum1), Budiatman Satiawihardja1,2) dan Harsi D. Kusumaningrum1,2,3)*1)Program Studi Ilmu Pangan, Institut Pertanian Bogor, Bogor2)Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor3)Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Bogor Diterima 19 November 2013 / Disetujui 12 Mei 2014ABSTRACT Dangke is a traditional milk curd product, made by coagulation of milk using fresh papaya latex. This product is usually kept at room temperature (27-30ºC) until consumption. Dried papaya latex was used in this study to produce dangke, and its effect to S. aureus was determined by direct contact in TSB and dangke. Fresh papaya latex was dried using vacuum oven at 50-55ºC for 22 hours. Dried papaya latex at a concentration of 2.7x10-3 g/100 mL could reduce S. aureus approximately 1 log CFU/mL in TSB after 24 hours. Dried papaya latex and papain could maintain the S. aureus number in dangke within 24 hours storage at room temperature. The antibacterial activity of non-proteolytic compound of papaya latex, i.e ethanolic extract of papaya latex was determined by macrodilution method, resulted an the MIC90 of 8 mg/mL. The cell membrane leakage after exposure was detected by measuring the optical density of bacterial supernatant at 260 nm. The result showed that exposure to increasing antibacterial concentration resulted in increasing of optical density of S. aureus supernatant, indicating that the antibacterial caused the S. aureus membrane leakage. Fluorescence microscopy imaging showed that S. aureus exposure to antibacterial caused membrane leakage thus gave Propidium Iodide (PI) chance to penetrate into the cell, as indicated by changing of fluorescence color from green to red.

REDISTILAT ASAP CAIR CANGKANG KELAPA SAWIT SEBAGAI BAHAN PENGAWET BAKSO SAPI Suminar Setiati Achmadi; Harsi Dewantari Kusumaningrum; Ihsan Anggara
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Liquid smoke has been used to extend the shelf life of food. However, its composition varies considerably depending on the type of raw materials used and preparation precedure. Liquid smoke derived from palm oil shell is potential due to the abundance of its byproduct sources in the palm oil industry. This study thus aims to prepare the best fraction of liquid smoke that can extend the shelf life of beef meatballs at room temperature. The raw liquid smoke was redistilled at 80, 90, and 100°C and was used as an ingredient in the beef meatballs production. The gas chromatography-mass spectrometry identification showed that there were no harmful compounds such as derivatives of tar and polycyclic aromatic hydrocarbons. Liquid smoke produced from redistillation at 80°C had the best result for providing higher acid value and lower pH, i.e. 5.14% and 2.26, respectively. The LC50 value of the redistilled liquid smoke in brine shrimp lethality assay was 0.16%. Inhibition zones of 0.1 and 0.8% redistilled liquid smoke on the antibacterial test against Staphylococcus aureus were both 6.10 mm, while the zones of inhibition for Escherichia coli were 0 and 7.0 mm, respectively. These resulting inhibition zones were less effective than that of 100 ppm chloramphenicol, i.e. 14.2 mm on S. aureus and 12.6 mm on E. coli. The usage of redistilled liquid smoke at concentration of 0.8% in meatballs was found to inhibit total microbial growth greater than that of the addition at 0.1%. Moreover, the addition of redistilled liquid smoke inhibited the growth of microbial up to 18 hours at room temperature.

Co-Authors . Andriani . Nurhayati . Suliantari Achmad Poernomo Agung Santoso Aldilla S. Utami Andiarto Yanuardi Andriani . Andriani a Andriani Andriani Andriani Andriani Apon Zaenal Mustopa Apon Zaenal Mustopa Aris D. Toha Asep Wawan Permana Betty S. L. Jenie Betty Sri Laksmi Jenie Betty Sri Laksmi Jenie Betty Sri Laksmi Jenie Betty Sri Laksmi Jenie Budiatman Satiawihardja Eka Aditya Iskandar Eni Kusumaningtyas Eni Kusumaningtyas Evi Savitri Iriani Hanifah Nuryani Lioe Hartati Chairunnisa Herwin Pisestyani Herza Govina Sobarsa I P G Arya Dharmawan Ihsan Anggara Imawati Eka Putri Jamal Zamrudi Kurnianto, Muhammad Alfid Kusmajadi Suradi Kusmajadi Suradi Lely Rahmawaty Lilis Nuraida Lita Handayani Maggy Thenawidjaja Suhartono Mirnawati Soedarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mona Nur Moulia N Nurhayati Nugraha E. Suyatma Nugraha Edhi Suyatma Nurhayati Nurhayati Nurhayati Nurhayati Palupi, Nurheni Sri Pika Mustika Puspo Edi Giriwono Raden Haryo Bimo Setiarto Rahmatia Garwan Ramadani, Suci Putri Raphaella Widiastuti Ratih Dewanti -Hariyadi Reni Nofrianti Retnani Rahmiati Reza Fadhilla Rifah Hestyani Arum Rika Puspitasari MZ Rizal Syarief Sindhu H. Putra Siti Mariana Widayanti Sri Budiarti Poerwanto Sri Wahyuningsih Sri Widowati SUMINAR SETIATI ACHMADI Surachmi Setiyaningsih Syahrizal Nasution TATI NURHAYATI Tatik Khusniati Taufikkilah Romadhon Uswatun Hasanah Wendry Setiyadi Putranto Widya Eka Prayitno Winiati P Rahayu Winiati P Rahayu Winiati Pudji Rahayu Yusma Yennie Zakiah Wulandari

Title

Found 37 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 37 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 37 Documents
Search