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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Biota Jurnal Bioteknologi & Biosains Indonesia (JBBI) JURNAL PANGAN
Sukarno Sukarno
Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Karakterisasi Distribusi Ozon Pada Air Distilasi dan Air Kelapa yang Diproduksi Menggunakan Mesin Lucutan Plasma Berpenghalang Dielektrik Ganda David Yudianto; Ratih Dewanti Hariyadi; Sukarno Sukarno; Muhammad Nur; Eko Hari Purnomo
Biota Vol 9 No 2 (2023): Jurnal Biota 2023
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v9i2.16714

Abstract

Ozone produced using plasma technology can act as an antimicrobial agent that could be applied in a sterilization process. A Double Dielectric Barrier Discharge (DDBD) machine has ability to produce ozone in sufficient amount for microbial inactivation. The objective of this study is to characterize the ozone distribution expressed as dissolved ozone in distilled water and coconut water produced using a DDBD machine. The information can be useful for industries to design a commercial sterilization process. The results shows that an oxygen flow rate of 0.2 L/min produces the highest ozone concentration, i.e. 3440 mg/L. In addition, the capacity of the machine is relatively similar to all off oxygen flow rate, i.e., 41.28-43.2 g/hour. The oxygen flow rate of 0.2 L/min produces the highest dissolved ozone concentration, i.e. 0.42 mg/L distilled water. This oxygen flow rate is followed by its best kinetic model based on its linear portion during dissolved ozone penetration. A zero order model could describe this penetration process well, including its best k value of 0.0292 mg/L per minute with the highest dissolved ozone concentration among other oxygen flow rates. Besides, distilled water could represent dissolved ozone penetration in coconut water. Ozone gas and dissolved ozone concentration have possitive corellation with R-square value of 0.8934.
Isolasi, Identifikasi, dan Produksi Miselia Rhizopus sp. Berkadar Asam Nukleat Rendah untuk Pengembangan Mikoprotein Riza Firmansyah; Nampiah Sukarno; Utut Widyastuti Suharsono; Sukarno Sukarno; Wendi Nurul Fadillah
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.179

Abstract

Fungi, including Rhizopus sp., are food sources that commonly contain high nucleic acid levels. Therefore, the nucleic acid content must be reduced to achieve health standard requirements. This study aimed to isolate, identify, and produce Rhizopus sp. mycelium containing low nucleic acid. The Rhizopus spp. were isolated from tempeh collected from 12 different areas in Indonesia. Fungal identification was conducted based on morphological characteristics. The fungal isolates were selected based on mycelial growth and spore production on PDA. Biomass production of mycelium was carried out in potato extract and soybean extract media obtained from 200 g/L and 333.3 g/L, respectively. In each medium, 6 sugar levels were added, namely 0, 2, 3, 4, 5, and 6 g/L. Mycelium nucleic acid content reduction was achieved by heat treatment at 50°C and 60°C for 15 minutes and measured by a spectrophotometer at 260 nm. Fifty-eight isolates that were identified into 3 species were obtained in this experiment: R. oryzae, R. stolonifera, and R. microsporus. R. Microsporus had higher mycelium biomass and lower spore number than the other species. R. Microsporus produced a higher mycelium biomass in the soybean extract medium with 5 g/L additional sugar. The nucleic acid content of the 50°C heat-treated mycelium was 1.82% and 1.73% at 65°C. These values fulfilled the standard of mycelial nucleic acid content permitted in food by the USDA. Keywords: morphology, Rhizopus microspores, spore, soybean extract, tempeh
Co-Authors . Anidah . Aslan Afwa Nururrahmah Munawaroh Ardiansyah, Ardiansyah Arif Hartoyo Armaiki Yusmur Azis Boing Sitanggang Budi Suarti Candytias Puspitasari David Yudianto Dedi Fardiaz Dwyana Izza Augusta Eko Hari Purnomo Eris Septiana Erma Rohmawati Fauzia Nurul Izzati Febriansyah, Muhammad Irfan Ferdina Tri Laksmita Muhammad Ilman Muhammad Nur Nadia T. Hendartina Nampiah Sukarno Nancy Dewi Yuliana Nova Kushandita Partomuan Simanjuntak Rahmawati, Siti Irma Ratih Dewanti -Hariyadi Rikno Harmoko Riza Firmansyah Saiful Bachri Salcia Inka Nelanda Slamet Budijanto Slamet Budjianto Tatty Yuniati Utut Widyastuti Suharsono Wendi Nurul Fadillah