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The Relationship Between Soil-transmitted Helminth Infections and Clean and Healthy Living Behavior Anifa Chofsoh Zuchaliya; Yulia Sari; Sigit Setyawan; Yusuf Ari Mashuri
Disease Prevention and Public Health Journal Vol. 15 No. 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.3159

Abstract

Background: Soil-transmitted Helminth (STH) are nematodes that can infect humans through direct contact with soil due to poor hygiene behavior. School-age children are the most susceptible to the infection. The clinical symptoms of STH infection are usually asymptomatic. However, it can affect the quality of life of the infected. This study aims to analyze the relationship between STH infection with clean and healthy living behavior; Method: This study takes the form of analytic observational research with a cross-sectional approach. The sampling technique used was the total sampling method with subjects of 132 students of Sekolah Dasar (SD) 1 Talakbroto, SD 1 Kedunglengkong, and SD Wates, Simo, Boyolali. The data were obtained from the results of the students' clean and healthy living behavior questionnaire and the examination of stool samples using the Kato Katz technique. Data were presented in a categorical form and analyzed using the chi-square method.; Results: There were 15 students (11%) infected by STH from 132 subjects studied. Based on the chi-square test, the p-value of STH infection and clean and healthy living behavior was 0.043 (p<0,05); Conclusion: There is a significant relationship between STH infection with clean and healthy living behavior. 
Toxoplasma gondii Coinfection in HIV/AIDS Patients of Dr. Moewardi Hospital in Surakarta Irvan Raharjo; Yulia Sari; Afiono Agung Prasetyo
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Coinfection of T. gondii and HIV/AIDS will result in severe clinical symptoms. Age and gender are risk factors that are often associated with the occurrence of this coinfection. This study aimed to determine the seroprevalence of T. gondii and HIV/AIDS coinfection as well as to determine its association with age and gender. Methods: Serological test, ELISA, was performed in order to determine the presence of IgM anti-Toxo and IgG anti-Toxo in 51 blood samples taken from HIV/AIDS patients at Dr. Moewardi Hospital in November 2011. Results: The positive rates of IgM anti-Toxo and IgG anti-Toxo were 25.5% (13/51) and 17.6% (9/51), respectively. IgM anti-Toxo was associated with age ? 37 years (aOR 9.34 (95% CI 1.77549.174; p = 0.008)). Unless otherwise stated, no statistical association could be drawn. Conclusions: Screening on T. gondii infection is recommended for all HIV/AIDS patients since the positivity rates of anti-Toxo antibodies were found high in these patients. Keywords: Toxoplasma gondii, HIV/AIDS coinfection
Antifungal Effect of Ascaris suum, Goeze Pseudocoelom and Perienteric Body Fluid to Candida albicans, (C.P. Robin) Berkhout In Vitro Zakka Zayd Zhullatullah Jayadisastra; Yulia Sari; Sri Haryati
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Ascaris suum, Goeze pseudocoelom and perienteric body fluid is suggested to have antifungal effect to Candida albicans, (C.P. Robin) Berkhout because of Ascaris suum antibacterial factor (ASABF) which presents in pseudocoelom body fluid and neuropeptide-like protein 29 and caenacin-2 which present in perienteric body fluid. This research is conducted to prove the antifungal effect of Ascaris suum, Goeze pseudocoelom and perienteric body fluid to Candida albicans, (C.P. Robin) Berkhout in vitro. Methods: This was a quasi-experimental research. Candida albicans, (C.P. Robin) Berkhout speciments were tested using Ascaris suum, Goeze pseudocoelom and perienteric body fluid with concentration of each were 80%, 90%, and 100%. Specimens were incubated in temperature of 37oC for 24 hours and the inhibitory zone was measured. Result: Ascaris suum, Goeze pseudocoelom and perienteric body fluid didnt create any inhibitory zone in Candida albicans, (C.P. Robin) Berkhout inoculation. Conclusion: Ascaris suum, Goeze pseudocoelom and perienteric body fluid didn't have antifungal effect to Candida albicans, (C.P. Robin) Berkhout. Keywords: Candida albicans, Ascaris suum, Pseudocoelom Body Fluid, Perienteric Body Fluid.
Interleukin-10 of Positive and Negative HIV RNA Patients in Dr. Moewardi General Hospital in Surakarta Emirita Wulan Pradeta; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: HIV infection may cause imbalance of immune system and impacts on IL-10 status which has several immunological roles that may affect HIV infection itself. The aim of this study was to investigate the association between IL-10 and HIV RNA status. Methods: The IL-10 levels in the plasma samples obtained from 60 HIV patients in Dr. Moewardi General Hospital in Surakarta were analyzed by ELISA (Enzyme-Linked Immunosorbent Assay). Results: Elevated IL-10 level was found in 21.7% (13/60) samples and predominantly in positive HIV RNA (OR 2.67; 95% CI 0.40-17.96) and male (OR 1.22; 95% CI 0.36-4.17) patients. Conclusion: Elevated IL-10 level was associated with positive HIV RNA status and gender. Keywords: IL-10, HIV RNA