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All Journal HAYATI Journal of Biosciences Agrikultura Biotika: Jurnal Ilmiah Biologi Journal of Marine Research Cropsaver : Journal of Plant Protection Jurnal Perikanan dan Kelautan Journal of Coastal and Ocean Sciences Jurnal Teknologi Hasil Peternakan Journal of Health Technology Assessment in Midwifery Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Mia Miranti Rustama
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Physics Public Health Veterinary

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Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) ., Melanie; Rustama, Mia Miranti; Sihotang, Inriyani Sintia; Kasmara, Hikmat
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.668 KB)

Abstract

Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti.  B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti  resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis  that  non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation
Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) Melanie Melanie; Mia Miranti Rustama; Inriyani Sintia Sihotang; Hikmat Kasmara
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.668 KB) | DOI: 10.24198/cs.v1i1.16999

Abstract

Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti.  B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti  resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis  that  non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation
UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK AIR DAN ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI GRAM NEGATIF DAN GRAM POSITIF YANG DIISOLASI DARI UDANG DOGOL (Metapenaeus monoceros), UDANG LOBSTER (Panulirus sp), DAN UDANG REBON (Mysis dan Acetes) Martha Elselina Lingga; Mia Miranti Rustama
BIOTIKA Jurnal Ilmiah Biologi Vol 5, No 2 (2006): BIOTIKA DESEMBER 2006
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v5i2.337

Abstract

EFEKTIVITAS FORMULASI Helicoverpa armigera Nuclear Polyhedrosis Virus SUBKULTUR (HaNPV1 ) DALAM BERBAGAI BAHAN PEMBAWA TERHADAP MORTALITAS DAN PERTUMBUHAN Crocidolomia pavonana Fabricius Amalia Fildzah Tamii; Mia Miranti Rustama; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10073

Abstract

EFEKTIVITAS FORMULASI Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) HASIL SUB-KULTUR TERHADAP BERAT MAKSIMUM DAN LOLOS HIDUP LARVA Plutella xylostella Fuji Hardiani; Melanie Melanie; Mia Miranti Rustama
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10074

Abstract

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK AIR DAN ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI GRAM NEGATIF DAN GRAM POSITIF Mia Miranti Rustama; Sri Rejeki Rahayuningsih; Joko Kusmoro; Ratu Safitri
BIOTIKA Jurnal Ilmiah Biologi Vol 4, No 2 (2005): Biotika Desember 2005
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v4i2.303

Abstract

EFEKTIVITAS FORMULASI Helicoperva armigera NUCLEAR POLYHEDROSIS VIRUS HASIL SUB KULTUR (HaNPV ) TERHADAP MORTALITAS, WAKTU KEMATIAN DAN BERAT BADAN LARVA Spodoptera litura Fabricius (1775) Fitriyani Fitriyani; Mia Miranti Rustama; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i2.10079

Abstract

Pengaruh Suhu Pengeringan Polyhedra Terhadap Efektivitas Helicoverpa Armigera Nuclear Polyhedrosis Virus (HaNPV) Korespondensi: Dr. Mia Miranti, MP. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran, emal : miamiantariksa@gmail.com. Subkultur dalam Mengendalikan Populasi Spodoptera litura Fabricius Putri Marissa; Mia Miranti Rustama; Wawan Hermawan
BIOTIKA Jurnal Ilmiah Biologi Vol 11, No 1 (2013): BIOTIKA JUNI 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v11i1.10041

Abstract

Penelitian mengenai pengaruh suhu pengeringan polyhedra terhadap efektivitas Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) subkultur dalam mengendalikan populasi Spodoptera litura Fabricus telah dilakukan. Penelitian dilakukan dengan metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal dengan 5 taraf perlakuan (HaNPV cair dan HaNPV yang dikeringkan pada suhu 4oC, 25C) dengan empat kali pengulangan. Hasil penelitian menunjukkan bahwa berat badan maksimum larva S. litura instar tiga mencapai 0,967 g terjadi pada larva yang terinfeksi HaNPV yang dikeringkan pada suhu 4C dan pertambahan berat badan mencapai 0,732 g/ekor/hari pada larva yang terinfeksi HaNPV yang dikeringkan pada suhu 4C
UJI VIRULENSI BAKTERI Aeromonas hydrophila PADA IKAN KOMET (Carrasius auratus auratus Linnaeus, 1758) Rico ‎Rico; Mia Miranti Rustama; Ujang Subhan
BIOTIKA Jurnal Ilmiah Biologi Vol 19, No 1 (2021): BIOTIKA JUNI 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v19i1.34411

Abstract

Ikan komet (Carrasius auratus auratus Linnaeus, 1758) merupakan ikan hias yang banyak dibudidayakan. Salah satukendala dalam budidaya ikan komet adalah serangan penyakit yang disebabkan oleh bakteri Aeromonas hydrophila.Penelitian ini bertujuan untuk mengetahui virulensi bakteri A. hydrophila pada ikan komet. Penelitian menggunakanmetode deskriptif eksploratif. Data hasil dianalisis secara deskriptif. Kepadatan bakteri yang digunakan pada uji perendaman sebesar 1,58×10⁷ cfu/ml dan 5,08×10¹¹ cfu/ml. Pengamatan tanda klinis dan mortalitas ikan dilakukan setiap12 jam selama 96 jam. Hasil penelitian menunjukkan bahwa ikan komet yang terinfeksi A. hydrophila dengan kepadatanberbeda akan menunjukkan tanda klinis dengan tingkat keparahan yang berbeda. Bakteri A. hydrophila dengan kepadatan5,08×10¹¹ cfu/ml mengakibatkan kemunculan tanda klinis yang parah dan bakteri dengan kepadatan 1,58×10⁷ cfu/mlmengakibatkan tingkat kelangsungan hidup (survival rate) yang rendah. Pada penelitian ini bakteri A. hydrophila yangmenginfeksi ikan komet memiliki tingkat virulensi yang tinggi.
Characterization of Bacillus and Lactobacillus Encapsulated in Various Carrier Materials for Vannamei Probiotics (Litopenaeus vannamei Boone, 1931) Yuli Andriani; Aufa Aulia Kanza; Mia Miranti Rustama; Ratu Safitri
Jurnal Perikanan dan Kelautan Vol 7, No 2 (2017)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v7i2.2684

Abstract

Bacillus and Lactobacillus are bacteria that can be used in dry-stock probiotics in vannamei shrimp feed. In this study, it was performed to encapsulate Bacillus and Lactobacillus probiotic bacteria in several carrier materials, including rice flour, talc, and maltodextrin. The purpose of this study was to determine the type of carrier material that can provide the best probiotic character in the encapsulation process which will then be used in vannamei shrimp feed. The method used in this study is experimental method. The study was conducted with the preparation stage and the initial probiotic characterization test. Furthermore, Bacillus and Lactobacillus probiotic encapsulation process used spray drying method to produce powder inoculum, as well as advanced characterization test of bacteria that have been encapsulated. The parameters observed were the viability, resistance to temperature, pH and bile salts. Data were analyzed using ANAVA and Duncan Multiple Range Test. The results showed that all types of carrier materials used maintained the probiotic character of Bacillus and Lactobacillus after the encapsulation process. This can be seen from the character of probiotics that are in good category and deserve to be used as a probiotic agent after encapsulation process in the material of rice flour, maltodextrin, and talc.
Co-Authors ., Melanie Aliifah, Felicia Amalia Fildzah Tamii Amalia, Eri Aufa Aulia Kanza Camellia Panatarani Dadan Sumiarsa Desak Made Malini Dikdik Kurnia Febri Doni Fitriyani Fitriyani Fuji Hardiani Hikmat Kasmara Hikmat Kasmara I Made Joni Inriyani Sintia Sihotang Iskandar Iskandar Joko Kusmoro Kalasuba, Karina Martha Elselina Lingga Mega Fatimah Rosana Melanie Melanie Melanie Melanie, Melanie Muhamad Fatah Wiyatna Putri Marissa Putri, Agni Annisa Putri, Cindy Meylia Ratu Safitri Rico ‎Rico Shobihah, Hasna Nabila Sihotang, Inriyani Sintia Siswina, Tessa Siva Siti Patimah Sri Rejeki Rahayuningsih Syaputri, Yolani Teguh Husodo Tri Mayanti Ujang Subhan Wawan Hermawan Wawan Hermawan Wendry Setiyadi Putranto Wiyatna, Muhammad Fatah Yuli Andriani Yuli Andriani Zidni, Irfan