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Journal : Biocelebes

INOVASI UBI KAYU TRANSGENIK MENGGUNAKAN TEKNOLOGI CRISPR-CAS9 SEBAGAI UPAYA PEMENUHAN KEBUTUHAN PANGAN MASA DEPAN May Ningsih Wigati; Nurul Fajar Amini; Esna Dilli Novianto
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16112

Abstract

Jumlah penduduk yang kian meningkat menyebabkan kebutuhan pangan terus meningkat. Hal ini menyebabkan angka ketergantungan akan beras juga meningkat. Padahal hingga saat ini, untuk memenuhi kebetuhan bahan pangan beras, pemerintah masih harus melakukan impor. Untuk itu sebagai upaya menurunkan ketergantungan konsumsi beras masyarakat Indonesia, pemerintah menggalakkan program diverfisikasi pangan. Salah-satu komoditas yang difokuskan dalam program ini yaitu ketela pohon atau ubi kayu. Namun, dalam pemenuhan kebutuhan gizi, ketela pohon masih memiliki kandungan protein yang rendah. Untuk itu dilakukan genome editing dengan teknik CRISPR-Cas9. CRISPR-Cas9 ini adalah teknologi yang bekerja untuk pengeditan genom dengan kemampuan menemukan tempat tertentu di DNA, untuk memotong sebagian kecil DNA di tempat itu, dan mengedit DNA tersebut. Penerapan teknik CRISPR-Cas9 terhadap ketela pohon menghasilkan tanaman yang mengandung protein yang lebih tinggi.
PENGARUH PEMBERIAN LIMBAH BAGLOG JAMUR TIRAM PADA PERTUMBUHAN DAN HASIL TANAMAN CAISIM (Brassica juncea L.) Esna Dilli Novianto; Nurul Anindyawati; Nindya Nur Aina Kasih
Biocelebes Vol. 17 No. 1 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i1.16405

Abstract

Caisim (Brassica juncea L.) is one of the most popular vegetable crops in the community. However, the production of caisim is not commensurate with the amount of consumer demand. Currently, one of the efforts made by farmers is to increase caisim production by adding inorganic fertilizers. This method can leave residues so that other alternatives are needed, such as the use of organic fertilizers that are more environmentally friendly. One of the potential sources of organic matter is oyster mushroom baglog waste because it contains a number of nutrients that can act as soil improvement materials. This study aims to study the effect of adding oyster mushroom baglog waste to the growth and yield of caisim (Brassica juncea L.) plants. This study used a factorial Completely Randomized Block Design with 4 treatments and 5 blocks. The treatments consisted of 0 g, 300 g, 600 g and 900 g of baglog oyster mushroom waste. The data obtained were analyzed for variance and continued with the orthogonal polynomial test. The results showed that the control treatment was better than the other treatments. It is suspected that the oyster mushroom baglog waste did not undergo a good fermentation process so that the content of C-organic, N, P and K was low which resulted in fewer nutrients being available for plants. Oyster mushroom baglog waste is not recommended for caisim cultivation practices.