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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
G. Ciptadi
Faculty of Animal Husbandry, Brawijaya University, Jl. Kampus Fapet UB, 65145, Malang
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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THE ESTIMATION ACCURACY OF LIVE WEIGHT FROM METRIC BODY MEASUREMENTS IN ONGOLE GRADE COWS Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H.F.N.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 3 (2013): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.3.149-155

Abstract

length in North Sulawesi province. Data on animal live weight (LW), body length (BL) and chest girth(CG) were collected from all cows (n=363) kept by traditional household farmers. Regression analysiswas carried out to estimate LW from all linear body measurements. Data were classified based on age ofanimals consisted of five groups with the first age group of two and half years old, to the fifth age groupof seven and half years old. The results showed that age significantly (P<0.05) influenced all bodymeasurements. Correlations between all pairs of measurements were highly significant (P<0.001) for allage groups. Regression analysis showed that live weight could be predicted accurately from chest girthand body length (R2=0.97). Multiple regression model can be recommended to predict live weight ofOngole grade cows based on those variables with their age groups ranging from 2.5 to ≥7.5 years old asfollows: Live weight (kg) = -806.410 + 4.79835 CG (cm) + 2.83500 BL (cm).
DETECTION OF MENDELIAN AND GENOTYPE FREQUENCY OF GROWTH HORMONE GENE IN ONGOLE CROSSBRED CATTLE MATED BY THE ARTIFICIAL INSEMINATION TECHNIQUE Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H.F.N.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.79-85

Abstract

The objectives of this study were to detect the Mendelian mode inheritance of growth hormone(GH) and to establish genotype frequency of GH gene in Ongole-crossbred cattle mated by the artificialinsemination (AI) technique. Total of 76 blood samples were collected from Ongole-crossbred cows andbulls (G0), and their progenies (G1) at the Tumaratas AI service center in North Sulawesi province,Indonesia. All blood samples were screened for the presence of GH locus using a PCR-RFLP methodinvolving restricted enzyme Msp1 on 1.2 % of agarose gel. Data were analyzed using statistical programfunction in Excel XP. The results showed that GH locus using alleles of Msp1+ and Msp1- enzymerestriction in Ongole-crossbred cows and bulls was inherited to their Ongole-crossbred progeniesfollowing the Mendelian mode inheritance. This Mendelian inheritance generated by AI technique wasnot under genetic equilibrium for the Msp1 genotype frequencies in groups of G0 and G1. The breedingprogram using genotypes of bulls and cows (G0) for generating the genotype of GH Msp1 enzymerestriction by AI technique should be maintained to increase these various allele dispersion rates forbreeding under genetic equilibrium of the Ongole-crossbred cattle population.
HERITABILITIES OF BODY SIZE BY GROWTH HORMONE (GH-Msp1) GENOTYPES USING PCR-RFLP IN ONGOLE GRADE CATTLE Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H. F. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.138-144

Abstract

Genotypic performance in term of heritability as the crucial factor of animal economical traits for body size inheritance had not been fully studied in Ongole-grade cattle. The objectives of this research were to define the heritability values of live weight, chest girth and body length in Ongole-crossbred cattle. Total of 37 blood samples were collected from parental cows and 2 blood samples from parental Ongole breed bulls. All blood samples were screened for the presence of growth hormone (GH) locus using PCR-RFLP method involving restricted enzyme Msp1 on agarose-gel (1.2%). Data were analyzed using statistical program in Excel XP. Results showed that the phenotypic estimation average of Ongole grade cattle population of live weight, chest girth and body length were 445.41 ± 45.95 kg, 175.35 ± 4.11 cm, and 139.70 ± 5.73cm, respectively. The heritability values of animal live weight, chest girth and body length in this study were 0.24, 0.003, and 0.41, respectively. These heritability values of animal live weight and body length would be categorized as moderate to high genotypic performance values, while the heritability of animal chest girth was included in low heritability standard of the animal economical trait performance.
The Quality of Gaga Roosters Semen During Cold Storage Using a Diluent Supplemented with Sorbitol Khaeruddin; Ciptadi, G.; Yusuf, M.; Fattah, A. H.; Junaedi; Syamsuryadi, B.; Wahjuningsih, S.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.436

Abstract

This study aimed to examine the effect of adding different concentrations of sorbitol to the Ringer’s lactate-egg yolk (RLEY) diluent on the quality of Gaga roosters’ semen during cold storage. A completely randomized design was used with 10 replicates and 4 levels of diluent treatment, including control, 1%, 2%, and 3% sorbitol. Semen was collected using a massage method, followed by fresh semen evaluation and dilution with a ratio of 1:5. Semen was stored at a cold temperature (5 ºC) and observed after 0, 24, 48, and 72 hours. The variables observed were semen pH, motility, kinematics, viability, plasma membrane integrity, acrosome integrity, malondialdehyde (MDA), and intracellular calcium ion (Ca2+) concentrations of sperm. All data were analyzed using analysis of variance (ANOVA). Subsequently, the evaluation of sperm mitochondrial activity and DNA damage was carried out, and the data were analyzed using the T-test. The results showed that treatment with sorbitol in the diluent had no effect on semen pH, kinematics (except straightness), acrosomal cap integrity, DNA damage, mitochondrial activity, and MDA concentration. However, sorbitol concentrations significantly affect (p<0.05) total motility, progressive motility, static motility, STR, viability, plasma membrane integrity, and intracellular Ca2+. In conclusion, the addition of 1-3% sorbitol preserves the quality of Gaga roosters’ semen during 72 hours of cold storage.
Co-Authors Fattah, A. H. H. F. N. Lapian, H. F. N. H.F.N. Lapian JUNAEDI Khaeruddin Khaeruddin L. Hakim M. Yusuf S. Wahjuningsih Syamsuryadi, B. U. Paputungan