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All Journal JURNAL KIMIA SAINS DAN APLIKASI ASPIRATOR Jurnal Bioteknologi & Biosains Indonesia (JBBI) Sains Natural: Journal of Biology and Chemistry Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)
Sogandi Sogandi
Universitas 17 Agustus 1945 Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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EFEK ANTIBAKTERI EKSTRAK DAUN CENGKEH (Syzygium aromaticum) TERHADAP Escherichia coli DAN Staphylococcus aureus Ardia Ramadhani; Susy Saadah; Sogandi Sogandi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.146 KB) | DOI: 10.29122/jbbi.v7i2.4146

Abstract

Antibacterial Effect of Clove Leaf Extract (Syzygium aromaticum) against Escherichia coli and Staphylococcus aureus Escherichia coli and Staphylococcus aureus are microorganisms that cause infection. Overcoming infection using antibiotics is known to generate bacteria that are resistant to some antibiotics, hence the need of other antibacterial resources. One of the natural sources that can be utilized is clove leaf (Syzygium aromaticum). This study aims to determine the types of compounds contained in clove leaves and their inhibitory activity against E. coli and S. aureus. The study began with extraction using maceration techniques, then the separation of the compounds through ethanol, n-hexane, and ethyl acetate fractionation. Next step was the identification of secondary metabolites of clove leaf compounds using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed the ethyl acetate fraction was the most active in inhibiting the growth of E. coli and S. aureus with a minimum inhibitory value (MIC) of 10%. The most dominant compound in the ethyl acetate fraction was found to be caffeine with a content of 23.36%.  Keywords: antibacterial, clove leaves, GC-MS, MIC, Syzygium aromaticum ABSTRAK Escherichia coli dan Staphylococcus aureus adalah mikroorganisme penyebab infeksi. Penanggulangan infeksi menggunakan antibiotik telah memunculkan bakteri yang resisten terhadap beberapa antibiotik sehingga perlu mencari sumber antibakteri lain. Salah satu bahan alam yang dapat dimanfaatkan adalah daun cengkeh (Syzygium aromaticum). Penelitian ini bertujuan mengetahui jenis senyawa yang terkandung dalam daun cengkeh dan aktivitas penghambatannya terhadap bakteri E. coli dan S. aureus. Penelitian diawali dengan ekstraksi menggunakan teknik maserasi, kemudian dilakukan pemisahan senyawa berdasarkan tingkat kepolaran melalui fraksinasi etanol, n-heksan, dan etil asetat. Hasil fraksinasi diujikan ke bakteri uji. Identifikasi senyawa metabolit sekunder daun cengkeh menggunakan Gas Chromatography Mass Spectrometry (GC-MS). Hasil penelitian menunjukkan bahwa fraksi etil asetat paling aktif menghambat pertumbuhan bakteri E. coli dan S. aureus dengan nilai kadar hambat minimum (KHM) 10%. Senyawa paling dominan pada fraksi etil asetat adalah kafein dengan kadar 23,36%.
Efek Larvasida Fraksi Etil Asetat Daun Bangun-bangun (Plectranthus amboinicus) terhadap Mortalitas Larva Aedes aegypti Sogandi Sogandi; Fadhli Gunarto
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 12 No 1 (2020): Jurnal Aspirator Volume 12 Nomor 1 2020
Publisher : Loka Litbang Kesehatan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.631 KB) | DOI: 10.22435/asp.v12i1.1288

Abstract

Abstract. Mosquitoes are insects that can transmit diseases to humans through their bites. Dengue haemorrhagic fever is one of the diseases that could be transmitted by Aedes aegypti mosquito. The use of insecticides to control the occurrence of dengue haemorrhagic fever builds resistance of the mosquito towards the chemical substance. Therefore, natural larvicide was used as an alternative to chemical insecticides. This research was conducted to study the effectiveness of larvicides from the ethyl acetate fraction of Bangun-bangun leaves (Plectranthus amboinicus) in killing the Aedes aegypti larvae and to identify the active larvicide compounds. This laboratory experiment research tested six extract concentrations (0.0; 1.0; 1.8; 3.2; 5.6; 10.0%) in three replications. Observations were administered for 48 hours to count the number of the dead Aedes aegypti instar III larvae obtained from the Entomology Laboratory of the Faculty of Veterinary, IPB. The results showed that the larvae mortality increased as the extract concentration increased. The percentage of Aedes aegypti killed reached 96% at an extract concentration of 10%. Meanwhile, the probit analysis showed that LC50 was found at a concentration of 5.56%. The identification of active compounds using GCMS revealed that the larvicidal compounds in ethyl acetate fraction were the ones from an organic acid group which were, stearic acid, and palmitic acid. Keywords: Aedes aegypti, identification, larvacidal, mortality Abstrak. Nyamuk merupakan serangga yang dapat menularkan penyakit melalui gigitannya. Salah satu penyakit yang ditularkan adalah demam berdarah dengue yang dibawa oleh nyamuk Aedes aegypti. Pengendalian demam berdarah dengue menggunakan insektisida berbahan kimia dapat menyebabkan resisten nyamuk terhadap insektisida meningkat, oleh karena itu larvasida alami diharapkan dapat menjadi alternatif pengganti insektisida kimia. Tujuan dari penelitian ini adalah mengetahui afektifitas larvasida dari fraksi etil asetat daun Bangun-bangun (Plectranthus amboinicus) terhadap mortalitas larva Aedes aegypti dan mengidentifikasi jenis senyawa aktif yang berperan sebagai larvasida. Penelitian ini merupakan eksperimen laboratorium dengan enam konsentrasi ekstrak (0,0; 1,0; 1,8; 3,2; 5,6; 10,0%) dan tiga kali ulangan. Pengamatan dilakukan selama 48 jam terhadap jumlah kematian larva Aedes aegypti instar III yang diperoleh dari Laboratorium Entomologi Fakultas Kedokteran Hewan, IPB. Hasil penelitian menunjukkan bahwa kematian larva meningkat seiring dengan meningkatnya konsentrasi ekstrak. Kematian Aedes aegypti mencapai 96% pada konsentrasi ekstrak 10% dan hasil analisa probit menunjukkan LC50 terdapat pada konsentrasi 5,56%. Identifikasi senyawa aktif menggunakan GCMS menunjukkan bahwa senyawa yang bersifat larvasida dalam fraksi etil asetat ini adalah senyawa dari golongan asam organik yaitu, asam stearate, dan asam palmitat. Kata Kunci: Aedes aegypti, identifikasi, larvasida, mortalitas
Isolation and Molecular Identification of Endophytic Bacteria of Clove Leaf (Syzygium aromaticum L) and Mechanism of Action Antibacterial Sogandi Sogandi; Irviyani Irviyani; Usep Suhendar
JURNAL SAINS NATURAL Vol. 12 No. 1 (2022): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1013.531 KB) | DOI: 10.31938/jsn.v12i1.326

Abstract

Endophytic bacteria are microorganisms that live in plant parts. Endophytic bacteria from clove (Syzygium aromaticum L.) leaves with antibacterial activity are rarely reported. Therefore, this study aims to isolate endophytic bacteria from clove leaves (Syzygium aromaticum L.), screen and determine potential endophytic bacteria as antibacterial compounds, identify molecularly with 16S rRNA, analyze bacterial growth curves and detect bioactive compounds produced by potential bacteria. Endophytic bacteria were isolated as many as 7 pure isolates including IRV1, IRV2, IRV3, IRV4, IRV5, IRV6, IRV7. IRV3 isolates showed the most dominant activity in inhibiting the growth of test bacteria and as the most potential producer of antibacterial compounds. Molecular identification with PCR (polymerase chain reaction) amplification in the 16S rRNA gene showed IRV3 isolate as Staphylococcus sp. with a 99% similarity value. Besides, the results of GCMS analysis of secondary metabolites of IRV3 isolates showed pyrazine content as a dominant compound, which has been known as a bioactive compound in inhibiting bacterial growth. Therefore, the results of this study revealed that IRV3 isolates that were successfully isolated and identified would be able to contribute to further research to find new antibiotic sources.Keywords: Antibacterial, Endophytic, Clove Leaves, Bioactive, Syzygium aromaticumIsolasi dan Identifikasi Molekuler Bakteri Endofit Daun Cengkeh (Syzigium aromaticum L) dan Mekanisme Aksinya Sebagai AntibakteriABSTRAKBakteri endofit adalah mikroorganisme yang hidup di dalam bagian tanaman. Bakteri endofit dari daun cengkeh (Syzygium aromaticum L.) dengan aktivitas antibakteri masih jarang dilaporkan. Oleh karena itu, penelitian ini bertujuan untuk mengisolasi bakteri endofit dari daun cengkeh (Syzygium aromaticum L.), skrining dan menentukan bakteri endofit potensial sebagai penghasil senyawa antibakteri, mengidentifikasi secara molekuler dengan 16S rRNA, menganalisis kurva pertumbuhan bakteri dan mendeteksi senyawa bioaktif yang diproduksi oleh bakteri potensial. Bakteri endofit yang berhasil diisolasi sebanyak 7 isolat murni diantaranya IRV1, IRV2, IRV3, IRV4, IRV5, IRV6, IRV7. Isolat IRV3 menunjukkan aktivitas yang paling dominan dalam menghambat pertumbuhan bakteri uji dan sebagai penghasil senyawa antibakteri yang paling potensial. Identifikasi molekuler dengan amplifikasi PCR (polymerase chain reaction) pada gen 16S rRNA menunjukkan isolat IRV3 sebagai Staphylococcus sp. dengan nilai similaritas 99%. Selain itu, hasil analisis GCMS dari metabolit sekunder isolat IRV3 menunjukkan kandungan pirazin sebagai senyawa dominan, yang telah dikenal sebagai senyawa bioaktif dalam menghambat pertumbuhan bakteri. Oleh karena itu, hasil penelitian ini mengungkapkan bahwa isolate IRV3 yang berhasil diisolasi dan diidentifikasi akan dapat berkontribusi pada penelitian lebih lanjut untuk menemukan sumber antibiotik baru.Kata kunci: antibakteri, endofit, daun cengkeh, bioaktif, Syzigium aromaticum
Antibacterial Activity and Mechanism of Action of Methanol Extract from Kasturi Mango Fruit (Mangifera casturi) on Caries-Causing Bacterium Streptococcus mutans Usep Suhendar; Muhammad Fathurrahman; Sogandi Sogandi
Jurnal Kimia Sains dan Aplikasi Vol 22, No 6 (2019): Volume 22 Issue 6 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3025.552 KB) | DOI: 10.14710/jksa.22.6.235-241

Abstract

One of the problems frequently found in the oral cavity is dental caries caused by Streptococcus mutans. Thus far, dental caries is treated using antibiotics. However, the bacterium is known to be resistant to many antibiotics; hence, another alternative is needed. An alternative option is found in the Kasturi mango (Mangifera casturi). This study aims to identify the bioactive compounds of  Kasturi mango and find out the mechanism of its action in inhibiting the growth of Streptococcus mutans. Kasturi mangoes were macerated using 96% methanol, then the phytochemical compounds were identified qualitatively. Antibacterial activity testing was carried out using the agar diffusion method, and bioactive compounds were identified using GCMS. The results showed that the methanol extract of Kasturi mango contains alkaloids, flavonoids, phenolics, terpenoids, and saponins. In 1 gr/mL of the methanol extract of Kasturi mango fruit has an inhibitory activity against the growth of Streptococcus mutans with a zone of inhibition of ± 10 mm and MIC (minimum inhibitory concentration) value of 25% extract. The inhibitory action is suspected to be through a mechanism where holes in the bacterial cell membrane are made. This can be seen from the results of SEM (scanning electron microscope) images showing that cell leakage or lysis has occurred. This research also, for the first time, revealed the types of bioactive compounds from the methanol extracts of Kasturi mango (Mangifera casturi) consisting of 18 compounds with the most abundance is 5-Hydroxymethylfurfural compounds, Octadecenoic acid, n-Hexadecanoic acid, Phenyl 4-methyl-1-piperidine carboxylate, and Methyl linolenate.
Identifikasi Senyawa Aktif Ekstrak Buah Mengkudu (Morinda citrifolia L.) dan Potensinya sebagai Antioksidan Sogandi Sogandi; Rabima Rabima
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2716.322 KB) | DOI: 10.14710/jksa.22.5.206-212

Abstract

Antioxidants are compounds that can inhibit oxidation reactions by binding to free radicals and highly reactive molecules. One form of reactive oxygen compounds is free radicals, these compounds are formed in the body and are triggered by various factors. Noni fruits have been used by the community as a medicine for high blood pressure, beriberi, urinating, inflammation of bile, inflammation of the intestine, dysentery, constipation, spleen pain, liver pain, diabetes and lumbago. Nevertheless, research on the identification of the active compounds of noni fruit which act as antioxidants yet. This study aims to extract and fractionation of Noni fruit, determine the antioxidant activity of Noni fruit extract, and identify the types of bioactive compounds that act as antioxidants. Noni fruit was extracted used maceration technique with 96% ethanol and then fractionated using n-hexane, chloroform and ethyl acetate solvents. Antioxidant activity from the results of noni fruit fractionation was measured by the DPPH (1.1 diphenyl-2-picrylhydrachyl) method and identification of bioactive compounds was carried out by GCMS. The results showed the greatest antioxidant activity was found in chloroform fraction with a percentage of inhibition of 78.19%. For the first time this study revealed the types of bioactive compounds from noni fruit ethyl acetate fraction which act as antioxidants are n-hexadecanoic acid, squalene, pyridin-3-carboxamide, oxime, n- (2-trifluoro methyl phenyl), and beta-sitosterol
IDENTIFIKASI KANDUNGAN GIZI DAN PROFIL ASAM AMINO DARI IKAN SELUANG [Rasbora Sp] (IDENTIFICATION OF NUTRITIONAL CONTENT AND PROFILES OF AMINO ACID FROM SELUANG FISH [Rasbora Sp]) Sogandi Sogandi; Rahmat Eko Sanjaya; Noor Baity; Syahmani Syahmani
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol. 42 No. 2 (2019): PGM VOL 42 NO 2 TAHUN 2019
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v42i2.1287

Abstract

Kalimantan Selatan sebagian besar wilayahnya berupa sungai dan rawa dengan luas daerah rawa mencapai 235.677 hektare. Dengan luas daerah seperti itu, Kalimantan Selatan memilki banyak jenis ikan sungai yang salah satunya adalah ikan seluang (Rasbora Sp). Melalui hasil penelitian ini diharapkan dapat meberikan informasi mengenai kandungan gizi, mineral, asam lemak dan jenis asam amino dari ikan seluang. Penelitian ini dimulai dengan (1) tahap pengambilan dan preparasi sampel, (2) analisis proksimat ikan seluang (kadar air, abu, protein, mineral dan lemak), (3) ekstraksi lemak ikan seluang, (4) analisis kandungan asam lemak dengan GC-MS dan (5) analisis jenis asam amino menggunakan HPLC. Ikan seluang (Rasbora Sp) diambil dari Sungai Barito Kabupaten Banjar, setiap 100 g ikan Seluang mengandung kadar air ±55,89%, kadar abu ±11,45%, total protein ±47,54%, total lemak ±12,36%, mineral Fe ±2,9%, Cu ±0,38%, Zn ±3,64% dan dalam 100 gram ikan seluang mengandung 21,53 kilokalori. Ikan seluang mengandung asam lemak oleat (omega 9) sebesar 36,43%, palmitat 27,43%, stearate 11,97%, palmitoleat 3,75%, laurat 3,17%, miristat 3,11%, dan EPA asam eikosapentanoat (Omega 3) sebesar 2,98%. Sedangkan jenis asam amino yang terdapat pada ikan seluang (Rasbora Sp) adalah 14 jenis terdiri dari 7 asam amino esensial (leusin, lisin, valin, isoleusin, phenil alanin, histidin, dan methionin) dan 7 asam amino non-esensial (asam glutamat, asam aspartat, serin, triosin, arginin, alanin, dan glisin).
Co-Authors Ardia Ramadhani Fadhli Gunarto Irviyani Irviyani Muhammad Fathurrahman Noor Baity Putu Nilasari Rabima Rabima Rahmat Eko Sanjaya Susy Saadah Syahmani Syahmani Usep Suhendar Usep Suhendar