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All Journal UNEJ e-Proceeding Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi UMI Medical Journal Molucca Medica
Siska Nuryanti
Fakultas Farmasi, Universitas Muslim Indonesia, Makassar
Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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KARAKTERISASI MAKROSKOPIK DAN MIKROSKOPIK SERTA ISOLASI DNA ISOLAT FUNGI ENDOFIT DAUN EKOR NAGA (Rhaphidophora pinnata L.F Schott) St Maryam; Siska Nuryanti; Kartini Eka Fakhriyanti Rahbuddin
As-Syifaa Jurnal Farmasi Vol 14, No 2 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i2.900

Abstract

 Endophyte fungi generally produce secondary metabolites which have biological activities such as anticancer, antiviral, or antibacterial compounds. The endophytic fungi isolate of FM 2 code is one of the endophytic fungi samples of dragon tail leaf (Rhaphidophora pinnata (L.f.) Schott) because it has the best activities. The research aimed to isolate DNA samples of the dragon-tail leaves and to identify the endophytic fungi isolates through macroscopic and microscopic observations. The significance of DNA isolation can be found through electrophoresis, which will show DNA band under the UV light. The results showed that the sample the endophytic fungi isolates on FM 2 code was identified conventionally by observing its morphological characters through macroscopic and microscopic identification compared to literature, showing the similarities to the genus Mucor sp. which will show DNA band under the UV light. The results showed that the sample of endophytic fungi was isolated by a transiluminator UV lamp indicating that the sample was successfully isolated
AKTIVITAS ANTIOKSIDAN FUNGI ENDOFIT HERBA KROKOT (Portulaca oleracea L.) Siska Nuryanti; Asriani Suhaenah; Rachmat Faisal Syamsu; Rini Andriani
As-Syifaa Jurnal Farmasi Vol 15, No 1 (2023): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v15i1.966

Abstract

Purslane herb (Portulaca oleracea L.) is one of the plants that is considered as a valuable plant in the pharmaceutical world for the future because of its antioxidant content and high nutritional value. Endophytic fungi can produce secondary metabolites that have activities similar to those produced by their host plants, where these metabolites have medicinal potential. In previous studies, it was found that the ethyl acetate extract of endophytic fungi had antidiabetic activity in the α-glucosidase enzyme inhibition test. This research aims todetermine the antioxidant activity of the ethyl acetate extract of the endophytic fungus of purslane herb (Portulaca oleracea L.) using the Test method1,1-diphenyl-2-picrylhydrazyl(DPPH). Purslane herbaceous endophytic fungus isolate (Portulaca oleracea L.) code FEK purified for 3 days. Then, fermented using MYB medium for 21 days. After that, extracted to become a thick extract. Furthermore, the DPPH test was carried out using a vitamin C comparison solution. Based on the IC50 value of the ethyl acetate extract of the endophytic fungus of purslane herb code FEK, it was found to be 64.26 μg/mL which is a relatively strong antioxidant activity
SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAUN KARET KEBO (Ficus elastica) DENGAN MENGGUNAKAN METODE PEREDAMAN RADIKAL BEBAS DPPH (2,2-Diphenyl-1- Picrylhydrazil) Asriani Suhaenah; Siska Nuryanti; Zainal Abidin; Hexatrianto Fatul Rahman
As-Syifaa Jurnal Farmasi Vol 15, No 1 (2023): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v15i1.935

Abstract

Free radicals are a form of reactive oxygen compounds, compounds that have unpaired electrons. Free radicals are independent atoms, molecules or compounds that have unpaired electrons, and therefore are highly reactive and unstable. Antioxidants are compounds that can slow down the oxidation process of free radicals. Ficus is a species that is rich in polyphenolic compounds, such as flavonoids which are strong antioxidants which can help in the prevention and treatment of various diseases caused by oxidative stress. The purpose of this study was to determine the value of antioxidant activity in the ethyl acetate fraction of kebo rubber leaves (Ficus elastica) using the DPPH (2,2-Diphenyl-1-Picrylhydrazil) free radical scavenging method based on the IC50 value. Based on the research results, it was found that the ethyl acetate fraction of kebo rubber leaves (Ficus elastica) contained flavonoids, phenolics, tannins and saponins and had antioxidant activity with an IC50 value of 70.74 µg/mL and included in the strong category
Co-Authors A. Rini Pratiwi Aan Yulianingsih Asriani Suhaenah Awaluddin Awaluddin Ayu Ulfiah Azis Burhanuddin Bahar Faridha Ilyas Firdaus Hamid Fitriana Fitriana Fitriana Fitriana Herwin Herwin Hexatrianto Fatul Rahman Kartini Eka Fakhriyanti Rahbuddin Muh. Farid Jamal Novita Prastiwi Rachmat Faisal Syamsu Rachmat Faisal Syamsu Reza Ariyadi Deponda Rini Andriani Rizalinda Sjahril Santri Santri St Maryam Tadjuddin Naid Zainal Abidin Zulhisda Premeita Sari