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Retno Damajanti Soejoedono
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Journal : Jurnal Veteriner

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PHYLOGENETIC AND ANTIGENIC STRUCTURE OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM WATERFOWLS R Susanti; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika; I Wayan Teguh Wibawan; Maggy Thenawidjaja Suhartono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried (1) to analyze the phylogenetic relationship of fragment hemaglutinin (HA) geneof avian influenza viruses (AIV) subtype H5N1 isolated from apparently healthy backyard waterfowls inWest Java with representative of animal and human isolates from Indonesia and some countries in Asia;(2) to find out cross-reactivity of those viruses with a standard Indonesian strain. Nucleotide sequences ofHA gene of AIV H5N1 from backyard waterfowls along with other H5N1 isolates of Indonesian and Asianorigin were aligned using with ClustalW of MEGA 3.1 program. Estimation of genetic distance and theconstruction phylogenetic tree were conducted by Neighbor Joining method and calculation of distancematrix using Kimura 2-parameter. Antigenic analysis was conducted using hemagglutination inhibition(HI) test. Result of phylogenetic analysis indicated that all viruses from backyard waterfowls form threedistinct sublineages. One lineage was located in Indonesia cluster and two lineages in Asia cluster. In thephylogenetic analysis, it was concluded that multiple introductions of AIV H5N1 to Indonesia have occurred.Six AI H5N1 viruses from backyard waterfowls (IPB1-RS to IPB6-RS) appeared to be different ancestorsthose isolated previously in Indonesia. Cross-antigenic analysis showed that nine viruses isolates used inthis study were antigenically different to Legok 2003 chicken strain of AIV H5N1. The HI titer of anti-Legok 2003 antibody with all newly isolated viruses is up to 6 log lower then the HI titer using homologstrain.
Phenotypic and Serotypic Characterization of Staphylococcus aureus Strains from Subclinical Mastitis Cattle (KARAKTERISASI SECARA FENOTIPE DAN SEROTIPE STAPHYLOCOCCUS AUREUS YANG BERASAL DARI MASTITIS SUBKLINIK PADA SAPI) Siti Gusti Ningrum; Wyanda Arnafia; Sylvia Oscarina; Retno Damajanti Soejoedono; Hadri Latif; Mohammad Ashraf; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also studied, such as erythromycin, streptomycin, tetracycline, cefepime,nitrofurantoin, amikacin, chloramphenicol, and ciprofloxacin. About 78% of the isolated were moderatelysensitive to nitrofurantoin, while 89% were highly resistant to cefepime and ciprofloxacin. Using thevarious mammals’ sera, seven isolates out of 104 revealed the presence of Spa.
Deteksi Virus Avian Influenza H5N1 pada Anak Ayam Umur Satu Hari dengan Teknik Imunohistokimia Sophia Setyawati; Retno Damajanti Soejoedono; Ekowati Handharyani; Bambang Sumiarto
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Influenza (AI) or bird flu caused by virus H5N1 is still present in Indonesia. The Department ofAgriculture of Indonesia has banned poultry distribution from endemic to non endemic area, except fordistribution of day old chick (DOC). The aim of this research was to detect AI virus infection in DOCdistributed from AI endemic to AI non endemic areas. Two hundred and forty DOCs from farms in WestJava and Banten were collected from Soekarno Hatta airport. Their antibody titers were examined againstAI virus by Haemaglutination Inhibition (HI) test. The AI virus detected in tissues (trachea, lung, heart,kidney, liver, and intestine) by immunohistochemistry technique. Detection of AI virus using anti AI H5N1monoclonal antibody was conducted AEC as chromogen. The result showed that 66,2% of DOC were positiveAI and 33,8% were negative AI. The 66,2% of positive samples, 43,3% showing the presence of AI antigenin trachea, lung and intestine, and 22,9% were presence in liver and kidney. DOCs were infected AI viruswith subclinical symptoms and they were potential as the source of rapid AI spread in Indonesia. It istherefore important to take a very cautious measure to prevent the spread of AI via DOC from AI endemicto free area.
Produksi IgY Antivirus Avian Influenza H5N1 dan Prospek Pemanfaatannya dalam Pengebalan Pasif I Wayan Teguh Wibawan; Sri Murtini; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Immunoglobulin Y (IgY) in yolk has been shown in several studies to prevent both bacterial and viralinfections. This research was conducted to find evidence that IgY specific against avian influenza virus(AIV) of H5N1 subtype can be produced in a large quantity in egg yolk. Laying hens were vaccinated withAI killed-vaccine (IPB-Shigeta). The IgY was purified using affinity chromatograpy technique, and anti-H5activity was measured using a standard haemaglutination inhibition (HI) and agar gel immunodifusion.The concentration of IgY was calculated, and the protein pattern was detected using polyacrilamid gel(AGID) electrophoresis (PAGE). Anti H5 antibody as high as 27 – 29 HI units was detected and produce aspecific line of precipitation in AGID. The concentration of IgY was 7.89 mg/ml. Purified specific IgY consistof 6 main protein bands with molecular weights ranging from 35 to 225 kD. These proteins were sensitiveto heat treatment (75oC for 30 minutes), to acid condition (pH2) as well as the pepsin and trypsin. Theseresults indicated the possibility of using specific IgY for passive immunisation to prevent AIV infection oras immunotherapeutic applications for AI treatment in humans.
Co-Authors Bambang Sumiarto Ekowati Handharyani Hadri Latif I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan Maggy Thenawidjaja Suhartono Mohammad Ashraf R Susanti Siti Gusti Ningrum Sophia Setyawati sri murtini . Sylvia Oscarina Wyanda Arnafia