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Total Phenolic, Flavonoid, Tannin Content and DPPH Scavenging Activity of Caesalpinia sappan Linn. Bark Brandon Aristo Verick Purba; Rini Pujiarti; Masendra Masendra; Ganis Lukmandaru
Wood Research Journal Vol 13, No 2 (2022): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2022.13.2.63-68

Caesalpinia sappan is a shrubby Leguminosae tree commonly found in Indonesia, traditionally utilized as natural dye and herbal drink. However, in the making of traditional herbal drink, the bark is often discarded as residues. This research aimed to investigate total phenolic (TPC), flavonoid (TFC), and tannin (TTC) content as well as the antioxidant activity (DPPH scavenging activity) of the bark successive extracted with n-hexane, ethyl acetate, methanol, and hot water as well as analyzed it with GC-MS. The result showed the highest amount of TPC (824.16±62.28 mg GAE/g), TFC (185.03±1.91 mg QE/g), and TTC (987.07±30.98 mg TAE/g) in the methanol extract of the bark. GC-MS analysis resulted hydroquinone as a major constituent in the methanol extract. Further, antioxidant activity was found the strongest on methanol extract (IC50=63.48), while correlation between antioxidant activity and TFC was found the highest (R2=0.93). These findings suggest that bark of C. sappan is a suitable source of natural antioxidant with strong activity to DPPH radical.

Investigation of Flavonoid Extractives and their Contribution to Color of Dalbergia latifolia Roxb Wood Masendra Masendra; Brandon Aristo Verick Purba; Denny Irawati; Ganis Lukmandaru
Wood Research Journal Vol 12, No 2 (2021): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2021.12.2.53-60

Dalbergia latifolia wood is a beautiful wood with color of dark purple to dark brown and rich in flavonoid compounds. This study aimed to investigate the presence of flavonoid compounds from D. latifolia wood and their contribution to its color. The D. latifolia wood sample was powdered and extracted with ethanol-toluene (1/2, v/v). The ethanol- toluene extract then was column chromatographed to obtain fraction 1 to fraction 12. Methylation was conducted to each fraction to detect flavonoid compounds by GC-MS. The results of GC-MS analysis showed that ethanol- toluene extract gave Fr- 1- Fr. 3 as soluble flavonoids (34.2%) and Fr. 4- Fr. 12 as insoluble polymers (62.6%). Furthermore, 12 flavonoids i.e latifolin, dalbergiphenol, chalcone, dalbergin, and their derivatives were characterised and these compounds might be significance in contributing to color of D. latifolia wood.

Phenolics Content and DPPH Free Radical Scavenging Activity of Dalbergia latifolia leaf ethanolic extract Masendra Masendra; Agus Ngadianto; Ridla Arifriana; Greitta Kusuma Dewi; Eko Prasetyo; Muhammad Rafly Nugraha; Saffana Ahda Labiba; Fatimah Ayu Warahapsari; Brandon Aristo Verick Purba; Ganis Lukmandaru
Wood Research Journal Vol 13, No 2 (2022): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2022.13.2.56-62

Dalbergia latifolia is one of the famous species that naturally grows in Java, Indonesia. The wood of this species is purple in color and the leaf is utilized as forage. The purpose of this study is to determine the bioactivity and phenol contents of D. latifolia leaf ethanolic extract. The antioxidant activity and anti-termite activity were measured through the DPPH method and Neotermes bosei activity. Furthermore, phenol contents were determined through colorimetric method and ethanolic extract constituents were identified by GC-MS. The DPPH inhibition of ethanol extract of D. latifolia leaf exhibited 138.20±2.14 µg/ml. While the measurement of total phenol and total flavonoid content of D. latifolia leaf showed a value of 192.67±9.41 mg GAE/ g and 55.23±5.11 mg QE/ g of dried extract sample. The termiticidal activity of ethanolic extract showed low activity. The GC-MS detection showed fatty acids as dominant compounds. The inhibition of DPPH by D. latifolia leaf ethanol extract in this study suggested this leaf is potent as antioxidant agents.

Total Phenolic, Flavonoid, Tannin Content and DPPH Scavenging Activity of Caesalpinia sappan Linn. Bark Brandon Aristo Verick Purba; Rini Pujiarti; Masendra Masendra; Ganis Lukmandaru
Wood Research Journal Vol 13, No 2 (2022): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2022.13.2.63-68

Caesalpinia sappan is a shrubby Leguminosae tree commonly found in Indonesia, traditionally utilized as natural dye and herbal drink. However, in the making of traditional herbal drink, the bark is often discarded as residues. This research aimed to investigate total phenolic (TPC), flavonoid (TFC), and tannin (TTC) content as well as the antioxidant activity (DPPH scavenging activity) of the bark successive extracted with n-hexane, ethyl acetate, methanol, and hot water as well as analyzed it with GC-MS. The result showed the highest amount of TPC (824.16±62.28 mg GAE/g), TFC (185.03±1.91 mg QE/g), and TTC (987.07±30.98 mg TAE/g) in the methanol extract of the bark. GC-MS analysis resulted hydroquinone as a major constituent in the methanol extract. Further, antioxidant activity was found the strongest on methanol extract (IC50=63.48), while correlation between antioxidant activity and TFC was found the highest (R2=0.93). These findings suggest that bark of C. sappan is a suitable source of natural antioxidant with strong activity to DPPH radical.

Evaluasi Aktivitas Antijamur dan Antioksidan dari Ekstrak Etil Asetat Kulit Kayu Pinus merkusii Masendra Masendra; Brandon Aristo Verick Purba; Ganis Lukmandaru
Jurnal Ilmu Kehutanan Vol 15 No 1 (2021): Maret
Publisher : Faculty of Forestry Universitas Gadjah Mada

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.

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