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All Journal Jurnal Kebidanan Jurnal Biosains Pascasarjana Biomedika The Indonesian Biomedical Journal Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Media Analis Kesehatan Jurnal Labora Medika Jurnal Analis Medika Biosains (JAMBS) Jurnal Biomedika Jurnal Hukum Kesehatan Indonesia Medika Respati : Jurnal Ilmiah Kesehatan

Budi Santosa

Department Of Medical/Clinical Laboratory Science, Universitas Muhammadiyah Semarang, Indonesia

Author-ID : 2390601

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Variasi Waktu Pembacaan Setelah Stop Solution Terhadap Nilai Absorbansi Anti HBS Metode Elisa Budi - Santosa
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 2 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i2.192

Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical technique used in the field of immunology to presence detection of antibodies or antigens in a sample. After providing a stop solution, it is necessary to know the right time for the absorbance reading. The purpose of this study was to determine the anti HBs titer with absorbance reading time variation (immediate, 30 minutes and 60 minutes). This research is an experimental study. The study population was D4 Health Analyst University Student Muhammadiyah Semarang Class 2018 after hepatitis B vaccination as many as 20. To know the difference in variation during the reading, Kruskal-Wallis test was conducted. The results showed the average absorbance at an immediate reading, 30, and 60 minutes complete in complete 1,931, 1,489, 1,276. Kruskal-Wallis statistical analysis obtained a p value of 0.00. The conclusion is obtained between the variation of absorbance reading time on the ELISA method

Comparison of Pap Smear Preparation Quality Containing Orange G and Without Orange G on the Results of the Papanicoulou Painting Intan Prihatin; Budi Santosa; Fennisia Wibisono
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.775

Examination procedure for pap smear preparation is influenced by fixation, drying and painting, and interpretation capability of examination. The Papanicolaou painting uses Harris Hematoxylin, Eosin Azure (EA) -50, and orange G. Orange G colors the orange matured and keratin cells Keratinizing-pink/orange cells. Chromatin in the nucleus will bind alkaline paint (hematoxylin) and cytoplasm protein will bind acidic paint (Orange G) and nucleus in the core will bind acid paint (EA 50) so that the cell will turn into pink with a blue core. The coloring stage according to procedure is using Orange G, but some PA laboratories do not use Orange G. The research goal is to know the comparison of pap smear preparation quality which contains orange G and without orange G towards papanicoulou painting result. The research type is experimental; the sample was 16 samples with two treatments namely coloring which using Orange G and without Orange G. The preparation quality which contains Orange G obtained moderate result of 12.5%, and good 87.5%. The preparation quality without Orange G was not good result of 6.3%, moderate 18.8%, and good 75%. The statistical test result of Chi Square and Kappa showed that the result was p>0.05 which means that comparison result of preparation quality which contains orange G and without orange G there was no significant difference.

PENGARUH KONSENTRASI KALIUM FEROSIANIDA TERHADAP PEWARNAAN Fe PADA SUMSUM TULANG Khoiriyatunnisa Khoiriyatuinnisa; Stalls Norma Ethica; Budi Santosa
Biomedika Vol 10, No 2 (2018): Biomedika Agustus 2018
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v10i2.7016

ABSTRAKPemeriksaan sumsum tulang merupakan uji diagnostik standar emas untuk penilaian cadangan zat besi di dalam tubuh, sehingga pemeriksaan ini dianggap pentingPemeriksaan cadangan besi sumsum tulang dalam prakteknya, ada beberapa variasi konsentrasi larutan kalium ferosianida (K4Fe(CN)6·3HO) yang digunakan.Faktanya, kalium ferosianida merupakan zat beracun yang berdampak buruk pada kesehatan jika terpapar pada konsentrasi tinggi. Di sisi lain, pengaruh konsentrasi kalium ferosianida terhadap kualitas hasil pewarnaan Fe belum pernah dilaporkan. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi kalium ferosianida terhadap kualitas hasil pewarnaan Fe berdasarkan interpretasi visual sebagai bagian dari pemeriksaan sumsum tulang. Secara lebih khusus, penelitian ini bertujuan untuk menentukan konsentrasi kalium ferosianida terendah dengan hasil kualitas pewarnaan Fe dengan interpretasi baik. Objek penelitian ini adalah sampel sumsum tulang pasien terdiagnosis anemia. Kemudian sampel diperiksa dengan pewarnaan Fe berbagai variasi konsentrasi kalium ferosianida, yaitu: 1 %, 2 %, 3 %, 4 %, dan 5% b/v (artinya b/v apa? Jangan menyingkat sebelum diberikan kepanjangannya). Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan pada hasil interpretasi tingkat cadangan besi akibat kualitas hasil pewarnaan yang berbeda akibat pengaruh variasi konsentrasi ferosianida dengan nilai uji signifikansi 0,00 (p 0,05) berdasarkan uji statistik Fisher. Dari hasil penelitian ini, konsentrasi kalium ferosianida yang paling direkomendasikan untuk pemeriksaan cadangan besi dalam sumsum tulang adalah 2% b/v, karena konsentrasi kalium ferosianidanya tidak terlalu tinggi namun menghasilkan kualitas penafsiran terbaik .Kata kunci: Pewarnaan Besi, Cadangan Besi Tubuh, Kalium Ferosianida, Sumsum Tulang ABSTRACTBone marrow examination is one of the most crucial diagnostic test considered as gold standard for the assessment of iron reserves in the body. In practice, there has been variation of potassium ferocyanide (K4Fe(CN)6·3HO) concentrations used in the iron staining step of the exam by medical laboratory workers. Infact, ferrocyanide is a toxic substance exposing health risks if used in high concentration. Yet, there have been no reports about the effect of ferrocyanide concentration on the quality of Fe staining results. This study aims to determine the effect of potassium ferrocyanide concentration on Fe staining result quality by visual interpretation as part of bone marrow examination. In more particular, it determines the lowest possible concentration (lowest toxicity) of ferrocyanide that could result in acceptable quality of Fe staining results. Objects of this study were bone marrow samples of patients diagnosed with anemia, which later examined by Fe staining using ferrocyanide at varied concentrations of 1, 2, 3, 4, and 5% b/v, respectively. Results indicated that there was significant difference on interpretation quality of iron reserves due to variation of ferrocyniade concentrations with significance test value of 0.00 0.05 based on Fisher’s test statistic. Results showed that the most recommended concentration of potassium ferrocyanide, which is not too high but resulting in the best possible interpretation quality of iron reserves on bone marrow is 2% b/v.Keywords: Fe Staining, Iron Reserves, Potassium Ferrocyanide, Bone Marrow

IR Bagendit Paddy Leaves Extract Improves Liver Cell Morphology and Reduces The Activity of Transaminase Enzymes After Lead Exposure in Rat Budi Santosa; Henna Ria Sunoko; Andri Sukeksi; Siti Thomas Zulaikhah
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.971

BACKGROUND: Lead (Pb) was known as one of systemic toxic agent. In the body, lead may be deactivated by the metallothioneins. Paddy leaves contain metallothioneins, sugars and pythosterols, and studies have shown the pharmacological activity of rice leaves on the protective effect of lead-induced rats against kidney function. The aim of this study was to evaluate the role of IR Bagendit paddy leaves extract as hepatoprotective agent.METHODS: Twenty-eight rats were divided into four groups: one control and three treatment groups. Control and treatment groups were exposed to lead of 0.5 g/kg body weight (BW)/day and then the treatment groups were administered with paddy leaves extract of 0.2; 0.4; and 0.8 g/kg BW/day per oral for 8 weeks. On the last day of the 8th week, body weight was measured and the numbers of normal, degenerative and necrotic liver cells were examined with hematoxylin-eosin staining. Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) were measured as liver function parameter. Difference of variables between control and treatment groups were examined by Friedman test.RESULTS: There was no association in different BW between groups. The normal liver cells are higher in treatment than control group (p<0.001) and necrotic liver cells are lower in treatment than control group (p≤0.001). There was no association in degenerative liver cells between groups (p=0.153). The activity of transaminase enzymes are lower in treatment than control group (p<0.001).CONCLUSION: IR Bagendit Paddy leaves extract reveals hepatoprotective activity by improving liver cells morphology and reducing the activity of transaminase enzymes after lead exposure.KEYWORDS: paddy leaves extract, liver normal cell, necrotic cell, transaminase enzymes

ANTIGEN NON STRUKTURAL 1 (NS1) SEBAGAI MARKER SUSPEK INFEKSI DENGUE Budi Santosa; Herlisa Anggraini; Arista Kurnia Budi Fristiani
Jurnal Media Analis Kesehatan Vol 11, No 1 (2020): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Infeksi dengue masih merupakan masalah kesehatan global. Angka insidensi dari tahun ke tahun belum mengalami penurunan. Angka kematian dari penderita cukup tinggi dan salah satu sebabnya adalah keterlambatan dalam penanganan penderita. Pemeriksaan laboratorium sangat dibutuhkan dalam menegakkan diagnosis dini penderita suspek dengue. Antigen non struktural 1 (NS1) dianggap sensitif spesifik bereaksi dengan virus dengue sehingga dapat mendeteksi keberadaan virus ini pada awal infeksi (suspek). Tujuan dari penelitian ini adalah untuk mengetahui gambaran hasil NS1 pada penderita suspek dengue. Penelitian ini menggunakan pendekatan deskriptif dari pasien yang diduga terinfeksi virus dengue. Tanda-tanda virus dengue diperoleh dari catatan medis yang berupa masa demam dan hasil Rumple Leed (RL) dari RS Roemani, hasil NS1 diperoleh dari pemeriksaan laboratorium. Hasil pnelitian menunjukkan dari 30 pasien yang suspek dengue dengan gejala demam 0-2 hari terdapat 14 orang, RL positif 3, NS1 positif 5. Gejala demam 3-4 hari terdapat 15 orang, 1 RL positif dan 1 NS1 positif. Gejala demem 5-7 hari terdapat 1 orang dengan hasil NS1 negatif. Simpulan bahwa tidak semua suspek dengue di RS Roemani didapatkan NS1 positif. Gejala demam 0-2 hari 35%, demam 3-4 hari 6,6%, demam 5-7 hari 0%.

UJI SKRINING FILARIASIS DI DESA JATIBARANG LOR KECAMATAN JATIBARANG KABUPATEN BREBES Asri Mulia Ningsih; Tulus Ariyadi; Budi Santosa
Jurnal Labora Medika Vol 4, No 1 (2020): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Penyakit filariasis adalah penyakit menahun menular yang disebabkan oleh cacing filaria yang ditularkan melalui nyamuk sebagai vektornya. Penyakit ini disebabkan oleh cacing filaria yang terdiri dari Wuchereria Bancrofti, Brugia Malayi dan Brugia Timori. Menurut data Dinas Kesehatan Kabupaten Brebes ditemukan 9 orang penderita filariasis di kecamatan Jatibarang. Tujuan pada penelitian ini adalah untuk mengetahui gambaran penyakit filariasis di desa Jatibarang Lor berdasarkan jenis mikrofilaria, jenis kelamin, umur, kebiasaan keluar rumah pada malam hari dan kebiasaan mengkonsumsi obat pencegah filariasis. Desain penelitian bersifat deskriptif dengan jumlah sampel sebanyak 30 sampel. Hasil penelitian tidak ditemukan adanya mikrofilaria, Berdasarkan karakteristik jenis kelamin paling banyak diikuti oleh perempuan sebanyak 16 orang. Berdasarkan karakteristik umur paling banyak diikuti pada umur dewasa dengan 22 orang sebanyak 73,3%. Berdasarkan kebiasaan keluar rumah pada malam hari didapat hasil baik dengan kebiasaan jarang keluar rumah dan berdasarkan kebiasaan minum obat pencegah filariasis didapat hasil 50% tidak pernah mengkonsumsi obat pencegah filariasis. Kesimpulan pada penelitian ini tidak ditemukan adanya mikrofilaria di desa Jatibarang lor.

Profesionalisme Tenaga Kesehatan dalam Penanganan Kegawatdaruratan COVID-19 Fitriani Nur Damayanti; Budi Santosa; Ta’adi Ta’adi
Jurnal MHKI Vol 2 No 01 (2022): April
Publisher : Masyarakat Hukum Kesehatan Indonesia

COVID-19 is an emergency that must be treated immediately by medical personnel. Handling the COVID-19 outbreak is the biggest challenge for health workers. Health workers need training for handling COVID-19 emergency cases because of the high risk in handling emergency cases. Patient safety is the main priority in handling emergency cases. The method used in this paper is a literature review study that aims to explore the professionalism of health workers in handling COVID-19 emergencies. The professionalism of health workers in handling COVID-19 emergencies consists of the main aspects, namely professional care in providing health services, competent health workers in handling COVID-19 emergencies, health workers having professional wisdom for handling COVID-19 emergencies, professional health workers having interpersonal competence, able to empower positive communication and partnerships with the patient's family, professional health workers develop themselves both personally and professionally, which is a prerequisite for true professionalism.

Blood Profile of Rattus Nurvegicus Exposed to HgCl2 and Received Combination of IR Bagendit Rice Leaves-Infused Water and Young Coconut Water Budi Santosa
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Mercury chloride (HgCl2) is widely used in industries; in fact, this chemical substance is deadly for health and causes various health problems, such as liver damage, kidney damage, and hematopoietic disorders, especially in erythropoiesis. This study aims to determine the blood profile in Rattus nurvegicus exposed to HgCl2 by combining IR Bagendit rice leaves-infused water and young coconut water. This study employed an experimental method to examine the positive control group exposed to HgCl2 and the negative control group receiving a placebo. Meanwhile, the treatment groups received a combination of IR Bagendit rice leaves-infused water in stratified compositions and young coconut water. The hematologic profile was examined using a hematologic analyzer, while the reticulocyte count was examined using wet and dry methods. The mean value of each group was tested using ANOVA. This study has successively obtained average levels of Hb (C+ 12,0;C- 14,2;T1 12,5;T2 12,9;T3 12,8), the erythrocyte count (C+ 6,52;C- 7,80;T1 1,44;T2 1,07;T3 7,32), levels of Ht (C+ 35,5;C- 42,9;T1 37,8;T2 37,9;T3 38,2), MCV (C+ 73,7;C- 79,9;T1 80,4;T2 78,7;T3 79,6), MCH (C+ 27,3;C- 29,9;T1 29,5;T2 28,5;T3 29,1), and MCHC (C+ 31,1;C- 34,2;T1 31,9;T2 33,3;T3 32,1), and the reticulocyte count (C+ 2,0;C- 1,2;T1 1,3;T2 1,1;T3 1,3). The significant differences in the groups are found in levels of Hb, Ht, MCV, and MCHC (p-value 0,00; 0,00; 0,03; 0,01). This study concludes that combining IR Bagendit rice leaves-infused water and coconut water could prevent blood profile exposure to HgCl2. Suggestions for further research to increase the intervention time and measure oxygen levels.

Evaluation in Hematology and BCR-ABL Molecular Profiles in Patients with Chronic Myeloid Leukemia Undergoing Tyrosine Kinase Inhibitor Therapy Muhammad Ihza Lisan Shidqi; Budi Santosa; Muhamad Muslim; Haitami Haitami
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Chronic myeloid leukemia (CML) is a myeloproliferative malignancy due to the formation of the BCR-ABL fusion gene in chronic myeloid leukemia. This condition causes excessive cell proliferation, resulting in an increase in the number of leukocytes. Tyrosine Kinase Inhibitor (TKI) is a first-line therapy that helps reduce the percentage of the Breakpoint Cluster Region–Abelson (BCR-ABL) fusion gene in patients with chronic myeloid leukemia. This study was conducted to determine evaluation in the hematological profiles (hemoglobin levels, leukocyte counts, platelet counts) and molecular BCR-ABL in patients with chronic myeloid leukemia before and after 12 months of tyrosine kinase inhibitors therapy. This analytic observational study was administered using a cross-sectional design to in analyzing the medical records of CML patients who underwent TKI therapy at the Sub Specialist Polyclinic of Internal Medicine Hematology Oncology Ulin Banjarmasin Indonesia Regional Hospital from March 2021-April 2022. Statistical test was performed which analysis results showed that 12-month tyrosine kinase inhibitor therapy could increase the hemoglobin levels, decrease leukocyte counts, platelet counts as well as decreasing the percentage of BCR-ABL gene fusion in patients with chronic myeloid leukemia. In conclusion, evaluation of tyrosine kinase inhibitor therapy in patients with chronic myeloid leukemia obtained significant differences in the hematological profiles and the molecular BCR-ABL. Further researchers are recommended to compare the type of tyrosine kinase inhibitor therapy between Imatinib and Nilotinib on the hematological and molecular profiles of BCR-ABL in patients with chronic myeloid leukemia with a larger sample count.

The Potential of Mangrove Stem Extract (Aegiceras corniculatum) on the Haematocrit Value Gustiadi Saputra; Theresia Indah Budhy; Muji Rahayu; Budi Santosa
Jurnal Biosains Pascasarjana Vol. 24 No. 2 (2022): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i2.2022.122-127

Hematocrit examination is an examination used to measure the concentration of red blood cells (erythrocytes) in the blood stated in percent (%). The usual hematocrit examination uses the anticoagulant EDTA, is expensive, and rarely distributed in remote area. Alternative anticoagulants are currently being studied, one of which is the extract of mangrove stems (Aegiceras corniculatum). This study aims to determine the difference in hematocrit values using the anticoagulant EDTA and extract of mangrove stems (Aegiceras corniculatum). The type of this research was analytic observational with a cross-sectional research design. Samples were taken by non-random purposive sampling of 16 female students from a total of 31 students of class D semester VIII of Health Analyst Universitas Muhammadiyah Semarang. The results showed average hematocrit value using EDTA anticoagulant was 42.38% by using the microhematocrit method. Meanwhile, the average hematocrit value using mangrove stem extract (Aegiceras corniculatum) is 20.88%. The hematocrit examination using the EDTA anticoagulant were higher than using the extract of mangrove stems (Aegiceras corniculatum). It showed a value of 0.000 with a significant level of 0.05, which is 0.000 <0.05. Therefore, there is a difference in hematocrit value using EDTA anticoagulant and mangrove stem extract (Aegiceras corniculatum).

Co-Authors Andri Sukeksi Arista Kurnia Budi Fristiani Asri Mulia Ningsih Fennisia Wibisono Fitriani Nur Damayanti Gustiadi Saputra Haitami Haitami Henna Ria Sunoko Herlisa Anggraini Intan Prihatin Khoiriyatunnisa Khoiriyatuinnisa Mudyawati Kamarudin Muhamad Muslim Muhammad Ihza Lisan Shidqi Muji Rahayu Nisa Julia Safarti Novita Nining Anggraini Purwanto Adhipireno Rina Febriyani Sarlince Agustin Nesan Siti Nurjanah Siti Thomas Zulaikhah Stalls Norma Ethica Ta’adi Ta’adi Theresia Indah Budhy Sulisetyawati Tulus Ariyadi Tulus Ariyadi Widya Cahya Maggyasari

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