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All Journal Microbiology Indonesia Jurnal Matematika & Sains Jurnal Sosioteknologi Biosaintifika: Journal of Biology & Biology Education AL-HAYAT: Journal of Biology and Applied Biology 3BIO: Journal of Biological Science, Technology and Management Makara Journal of Science Annales Bogorienses
Dea Indriani Astuti
Sekolah Ilmu dan Teknologi Hayati - Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Social Sciences

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Journal : Makara Journal of Science

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Indigenous Microbial Biostimulation for Microbial Enhanced Oil Recovery through Oil Degradation with Variation in Nutrent Concentrations Handaruni, Rika; Astuti, Dea Indriani; Purwasena, Isty Adhitya; Afifah, Lulu Nur
Makara Journal of Science Vol. 24, No. 2
Publisher : UI Scholars Hub

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Oil production in Indonesia has been declining since 2002, and this decline is incompatible with the high oil consumption in Indonesia. As such oil production in Indonesia should be improved. Biostimulation in microbial enhanced oil recovery involves environmental modification to stimulate microbial growth within a reservoir by adding limiting nutrients. Variation in C, N, and P concentrations injected to reservoirs can induce different responses from indigenous microbes and increase oil recovery. In this study, molasses, urea and diammonium phosphate were used as nutrients injected to a reservoir for biostimulation. Biological, physical and chemical characteristics after biostimulation were observed and bacterial growth was monitored up to 42 days. The physical characteristics observed were pH, oil viscosity, and interfacial tension. Gas chromatography mass spectrometry was performed to determine the chemical characteristics of oil. Results showed that the addition of nutrients at various concentrations yielded different production patterns of metabolites. The addition of urea and diammonium phosphate induced biosurfactants overproduction and increase hydrocarbon degradation of by bacteria. Therefore, hydrocarbons were degraded for the first 14 days, and polymerized again on days 14 to 42.
Sequential Isolation of Saturated, Aromatic, Resinic, and Asphaltic Fractions Degrading Bacteria from Oil Contaminated Soil in South Sumatera Munawar, Munawar; Aditiawati, Pingkan; Astuti, Dea Indriani
Makara Journal of Science Vol. 16, No. 1
Publisher : UI Scholars Hub

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Sequential isolation has been conducted to obtain isolates of saturated, aromatic, resin, and asphaltene fractions degrading bacteria from oil contaminated sites. Five soil samples were collected from South Sumatera. These bacterial isolates were obtained using soil extract medium enriched with oil recovery or remaining-oil recovery degradated (ROD) as sole carbon and energy sources according to the isolation stage as the isolation medium. ROD at the end of every isolation stage analyzed oil fractions by use of the SARA analysis method. Six isolates of bacteria have been selected, one isolate was fraction saturates degrading bacteria that are Mycobacterium sp. T1H2D4-7 at degradation rate 0.0199 mgs/h with density 8.4x106 cfu/g from stage I. The isolate T2H1D2-4, identified as Pseudomonas sp. was fraction aromatics degrading bacteria at accelerate 0.0141 mgs/h with density 5.1x106 cfu/g are obtained at stage II. Two isolates namely Micrococcus sp. T3H2D4-2 and Pseudomonas sp. T1H1D5-5 were fraction resins degrading bacteria by accelerate 0.0088 mgs/h at density 5.6x106 cfu/g and 0.0089 mgs/h at density 5.7x106 cfu/g are obtained at stage III. Isolation of stage IV has been obtained two isolates Pseudomonas sp. T4H1D3-1and Pseudomonas sp. T4H3D5-4 were fraction asphaltenes degrading bacteria by accelerate 0.0057 mgs/h at density 5.6x106 cfu/g and accelerate 0.0058 mgs/h at density 5.7x106 cfu/g.
Co-Authors Afifah, Lulu Nur ASEP RISWOKO DEWI NANDYAWATI Ferymon Mahulette, Ferymon Fitriagustiani Gede Suantika Handaruni, Rika Ikramullah, Muh. Chaeril IS HELIANTI Munawar Munawar Nasir, Fitri Handayani NIKNIK NURHAYATI Pasmawati PINGKAN ADITIAWATI Pingkan Adityawati PURWASENA, ISTY ADHITYA Putri, Nurul Febriani Sabatina, Grace Saepudin Saepudin Siti Eka Yulianti Taufik, Intan Yusup Sofyan