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All Journal KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Local Wisdom : Jurnal Ilmiah Kajian Kearifan Lokal Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdi Insani BIOEDUPAT: Pattimura Journal of Biology and Learning Pakem : Jurnal Pengabdian Kepada Masyarakat Rumphius Pattimura Biological Journal Kalwedo Sains (KASA) Berita Biologi
Ferymon Mahulette, Ferymon
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Microbiological and Physicochemical Characteristics of Inasua Traditional Fish Fermented from Maluku Islands Mahulette, Ferymon; Mubarik, Nisa Rachmania; Suwanto, Antonius; Widanarni, Widanarni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13537

Abstract

Based on the raw materials, inasua consists of two types namely inasua with sap and inasua without sap. Research of inasua with sap has never been done and considered as the novelty of this research. The sensory characteristics and shelf life of two types of inasua were different. The research aims to analyze the microbiological and physicochemical characteristics of two types of inasua during fermentation. The microbiological analyzes include the total number of bacteria and lactic acid bacteria, while physicochemical analyzes include temperature, pH, water activity, proximate analysis, salt, alcohol, histamine, amino acids and fatty acids contents. The total number of bacteria and lactic acid bacteria has decreased during fermentation. At the end of the fermentation the total number of bacteria and lactic acid bacteria inasua with sap were 3.2x107 CFU/g and 3.0x107 CFU/g, while inasua without sap were 5.4x105CFU/g and 3.5x105 CFU/g, respectively. The moisture, protein, alcohol contents and water activity decreased, otherwise the salt, fat, ash, amino acids, and fatty acids contents increased during fermentation. Generally, microbiological and physicochemical characteristics of inasua with sap was better than inasua without sap. The results of this research to improve the quality of this fermentation product in the future.
MICROBIOLOGICAL QUALITY AND PROXIMATE COMPOSITION OF BAKASANG LAOR, A TRADITIONAL FERMENTED FISHERY PRODUCT IN MALUKU Mahulette, Ferymon; Kurnia, Tri Santi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.399 KB) | DOI: 10.15294/biosaintifika.v12i1.23356

Abstract

Bakasang laor is one of the traditional fermented fishery products in Maluku, Indonesia. There are two types of  bakasang laor, i.e. with and without vinegar. The microbiological research of bakasang processed use laor as raw material  has never been done before. The research aimed to analyze the microbiological quality and proximate composition of two types of bakasang laor.  The proximate composition can determine the nutritional value of  this product for consumption by the community. The sample of bakasang laor was taken from traditional producer in Latuhalat village, Ambon. The total number of halotolerant and coliform bacteria  in bakasang laor without vinegar were 1.7x106 CFU/g and 4.1x106 CFU/g, respectively, while the total of lactic acid bacteria in bakasang laor with vinegar was 6.0x106 CFU/g.  The carbohydrate, protein, fat and ash contents of bakasang laor with vinegar was higher than bakasang laor without vinegar. The carbohydrate, protein and fat contents of bakasang laor with vinegar were 19.64 %, 16.23 %, and 2.42 %, respectively. Generally, microbiological quality and proximate composition  of  bakasang laor with vinegar was better than bakasang laor without  vinegar. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various food products.  The results of this research to improve the quality of this fermentation product in the future.
Microbial Succession and Chemical Characteristics in Fermentation of Ambonese Arrack (Sopi), Traditional Beverage from Maluku Mahulette, Ferymon; Astuti, Dea Indriani
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23791

Abstract

Ambonese arrack is one of the traditional fermented beverage product in Maluku, Indonesia. The microbiological research of this beverage that is processed using coconut sap as raw material has never been done before. The research aimed to analyze the microbial succession and chemical characteristics during fermentation of Ambonese arrack. The sample of coconut sap was taken from traditional producer in Mahia village, Ambon. The dominant microbes in the fermentation of Ambonese arrack were Pichia polymorpha and Kloeckera javanica. The highest numbers of these two microbes was obtained after 15 hours fermentation (9.6 log Cfu/mL and 9.9 Cfu/mL, respectively). The sugar content decreased from 593.3 mg/L to 474.3 mg/L, whereas ethanol content increased from 0.0018 g/L to 0.0100 g/L. The pH value decreased from 4.70 to 3.10. The research has isolated indigenous microbes in Ambonese arrack fermentation which was considered as novelty. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various beverage products, especially Ambonese arrack. The results of this research can improve the quality of this fermentation product in the future.
Ambonese Arrack (Sopi): Processing and It’s Functions in Moluccan Culture Mahulette, Ferymon
KOMUNITAS: International Journal of Indonesian Society and Culture Vol 13, No 1 (2021): March 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/komunitas.v13i1.25762

Abstract

Sopi is a beverage used in various traditional and religious ceremonies in Moluccas. The raw materials and processing techniques of this fermented product are very diverse. The processing of sopi has been done by the ancestors of Moluccan for a long time, but information about processing this product is very limited. This research aimed to describe the techniques of sopi processing and it’s functions in Moluccan culture. This study was descriptive qualitative research design. Data was collected from direct observations in five villages in differences islands, i.e. Mahia (Ambon island), Ouw (Saparua island), Sifluru (Ceram island), Wangel (Aru islands), and Yawuru (Kisar island). The processing of sopi were diverse in Moluccas. The raw materials used in the processing of sopi were saps from coconut, palm sugar, and coli trees, while the equipment of distillation were made of bamboo, plastic and pipe. Sopi was used in various traditional and religious ceremonies such as cuci negeri, panas pela, sumpah sopi and siram sopi. In general this traditional beverage is a symbol of solidarity in the Moluccans tradition. This research was expected to conserve of sopi processing culture to support the tourism in Moluccas. 
Traditionally Catching and Processing of Laor in Moluccas Islands Mahulette, Ferymon
Local Wisdom Jurnal Ilmiah Kajian Kearifan Lokal Vol. 12 No. 2 (2020): July 2020
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/lw.v12i2.4078

Abstract

Laor is a fishery product that found in Moluccas at March or April, a few days after the full moon. The swarming of laor in Moluccas is called timba laor. This event is one of the local wisdoms that must be conserved. The people have always consumed this worms for long time, but research of catching and processing techniques of laor has never been done. This research aimed to describe the techniques of catching and processing laor in Moluccas Province. This research was descriptive qualitative. Data was collected from direct observations and documentation from volunteers in several villages only in Moluccas Province, i.e. Latuhalat (Ambon), Booi (Saparua), Taruy (Geser), Elaar (Kei), and Emplawas (Tepa) villages. The catching and processing laor were very diverse in Moluccas. The people were usually catch of laor used a tanggo, both of triangular or oval shape. Laor that has been taken then processed into laor lawar and bakasang. Both of these food products were very popular in Moluccas community. This research was expected to conserve of timba laor culture to support the tourism in Moluccas province.DOI: https://doi.org/10.26905/lw.v12i2.4078
Processing of Inasua as Local Wisdom from Teon, Nila and Serua Communities in Ceram Island Mahulette, Ferymon; Kurnia, Tri Santi; Serpara, Ridolf; Rupilu, Marselina Ribka
Komunitas Vol 14, No 1 (2022): March 2022
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/komunitas.v14i1.30868

Abstract

Inasua is a traditionally fermented fish product which is local wisdom of Teon, Nila and Serua (TNS) Communities, Central Moluccas. Inasua processing initially using porkfish (Ruvettus tydemani Weber) as raw material. This fish is rarely found, so the people process inasua using a variety of reef fish. The processing of inasua has been done by the ancestors of the community for a long time, but information about processing this product is very limited. This research aimed to describe the techniques of inasua processing in Waipia, Ceram Island. This research was descriptive qualitative. Data was collected from direct observations in three villages representing three groups of TNS Communities, i.e. Layeni Village (Teonese), Kuralele Village (Nilanese), and Waru Village (Seruans). The processing of inasua was diverse in the three groups of TNS Communities. The people of Teonese usually process of inasua using only salt and salt added coconut sap, while the people of Nilanese using salt only. The people of Seruans usually process inasua using salt only, but some people also added coconut vinegar. This research was expected to conserve of inasua processing culture to support the tourism in Central Moluccas Regency.
Ambonese Arrack (Sopi): Processing and It’s Functions in Moluccan Culture Mahulette, Ferymon
Komunitas Vol 13, No 1 (2021): March 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/komunitas.v13i1.25762

Abstract

Sopi is a beverage used in various traditional and religious ceremonies in Moluccas. The raw materials and processing techniques of this fermented product are very diverse. The processing of sopi has been done by the ancestors of Moluccan for a long time, but information about processing this product is very limited. This research aimed to describe the techniques of sopi processing and it’s functions in Moluccan culture. This study was descriptive qualitative research design. Data was collected from direct observations in five villages in differences islands, i.e. Mahia (Ambon island), Ouw (Saparua island), Sifluru (Ceram island), Wangel (Aru islands), and Yawuru (Kisar island). The processing of sopi were diverse in Moluccas. The raw materials used in the processing of sopi were saps from coconut, palm sugar, and coli trees, while the equipment of distillation were made of bamboo, plastic and pipe. Sopi was used in various traditional and religious ceremonies such as cuci negeri, panas pela, sumpah sopi and siram sopi. In general this traditional beverage is a symbol of solidarity in the Moluccans tradition. This research was expected to conserve of sopi processing culture to support the tourism in Moluccas. 
ANALISIS KEBERADAAN BAKTERI ENTEROBACTER PADA AIR CUCIAN IKAN LAYANG (DECAPTERUS SPP) DI PASAR MARDIKA DAN PASAR TAGALAYA AMBON Manusiwa, Gellian Gabriella; Pattipeilohy, Mery; Pelamonia, Alamanda; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page42-51

Abstract

Background: Fish sales generally do not prioritize sanitation aspects in Ambon city. This study aims to determine the presence of Enterobacter bacteria and the morphological characteristics of Enterobacter bacteria in the washing water of flying fish (Decapterus spp) at Mardika Market and Tagalaya Market Ambon. Wash water samples were taken at Mardika and Tagalaya Markets in Ambon. Methods: The analysis used the Most Probable Number (MPN) method and biochemical tests (methyl red and citrate tests). Results: The results showed that 6 samples of flying fish washing water at the mardika market and tagalaya market that had been tested were identified as Enterobacter bacteria and based on the morphological characteristics found in the washing water samples of flying fish were pink, smooth edges, wavy, curved, and also not sequential and the elevation was flat and raised. The methyl red and citrate tests were negatife and positive respectively. Conclusion: Flying fish washing water at the Mardika and Tagalaya Markets has been contaminated with Enterobacter bacteria.
INTEGRATION OF PROJECT BASED LEARNING ETHNOSAINS TENUN IKAT TO IMPROVE UNDERSTANDING OF SCIENCE CONCEPTS AND CREATIVE THINKING STUDENTS JUNIOR HIGH SCHOOL (SMP) TANIMBAR Rupilele, Edy; Mahulette, Ferymon; Papilaya, Pamella Mercy
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p047-050

Abstract

This study aims to determine increased understanding of the concept of science through the integration of ethnoscience tenun ikat project based learning at SMP tanimbar, improving the ability to think creatively through the integration of the ethnoscience, differences in understanding the concept of science through the integration of project based learning ethnoscience, and differences in the ability to think creatively through the integration of ethnoscience. This research was conducted on April 26 – June 26 2021 located at SMPN 3 Wuarlabobar, SMPN 3 Waturu, SMP Christen Batuputih, SMPN 1 Atap Wermatang. The research design used a quasi-experimental design used two groups, namely the experimental group and the control group. The results of the study show that the integration of project based learning into the learning process can improve understanding of science concepts and ability to think creatively. The conclusion of this study is that there is a significant difference in the value of understanding science concepts and students' creative thinking ability between the sample groups of SMP Negeri 3 Wuarlabobar, SMP Negeri 3 Waturu, SMP Christen Batuputih and SMP 1 Atap Wertmatang.
ANTIBACTERIAL ACTIVITY OF BINAHONG ROOT EXTRACT (Anredera cordifolia (Ten.) Steenis) IN TREATMENT OF BOILS Pattiwael, Syane; Awan, Ali; Mahulette, Ferymon
RUMPHIUS Vol 4 No 1 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i1p018-025

Abstract

Infectious diseases caused by Staphylococcus aureus can be cured with traditional medicine, One example of a plant that has antibacterial power is binahong root. This study aims to determine the minimum inhibitory concentration (MIC) and minimum killing concentration (MBC) of binahong root extract against the growth of Staphylococcus aureus bacteria. Binahong root extract was obtained from maceration using absolute ethanol solvent. Furthermore, the extract obtained was made with a concentration dilution of 5%, 10%, 15% and 20%. The method used is disc diffusion. The results showed that each concentration of binahong root extract had an inhibitory effect on the growth of Staphylococcus aureus bacteria. This is influenced by the active compounds contained in the binahong root extract such as flavonoids, saponins, tannins, and alkaloids. Binahong root extract concentrations of 5%, 10%, 15%, and 20% were found to have zones of inhibition against the growth of S. aureus bacteria and the minimum inhibitory level (MIC) of Binahong root extract against the growth of S. aureus bacteria at a concentration of 5%.
Co-Authors Antonius Suwanto Awan, Ali Dea Indriani Astuti Elly, Sovian Sesca Hajan, Esmi Irwina, Irwina Kalkoy, Yosualda Kosaplawan, Martafina Kurnia, Tri Santi Lelapary, Claudia Manusiwa, Gellian Gabriella Matulessy, Yoin M Melay, Stevin Mery Pattipeilohy, Mery Muskita, Christo Nikijuluw, Monica NISA RACHMANIA MUBARIK Papilaya, Pamella Mercy Pattiasina, Eifan B Pattiwael, Syane Pelamonia, Alamanda Ratusehaka, Nopri Rupilele, Edy Rupilu, Marselina R Rupilu, Marselina Ribka Rupilu, Ronny Sahetapy, Elsa Josma Sangur, Kristin Sarak, Brian Serpara, Ridolf Simal, Rufiati Smith, Alwi Tuapattinaya, Prelly Marsell Jolanda Warella, Juen Carla Welerubun, Inggrid WIDANARNI WIDANARNI