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Haryanto Dhanutirto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Journal : Microbiology Indonesia

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Mutation and Characterization of an Albino Mutant of Monascus sp. Isolated from the Cikapundung River, Bandung TIANA MILANDA; MARLIA SINGGIH WIBOWO; TUTUS GUSDINAR; HARYANTO DHANUTIRTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.286 KB) | DOI: 10.5454/mi.1.1.5

Abstract

Monascus sp. isolated from Cikapundung River, Bandung was mutated using ethyl methanesulfonate (2.5%, 90 min). Previously, this wild type was identified as Monascus purpureus ITBCC-HD-F001 employing random amplification polymorphic DNA (RAPD). Stability of the mutant was observed using color consistency and mutant stability (sub-culturing for five generations) tests. Genetic variation of the mutant (M. purpureus ITBCC-HDF002) was confirmed by RAPD. One of the DNA bands of 1150 bp was found in the albino mutant but not in the wild type, so it was considered as a genetic variation resulting from the mutation process. The albino mutant was characterized by comparing the growth curve, biomass production curve, and the monascidin A production curve of both strains i.e. wild type and the albino mutant. Monascidin A production of the mutant was higher than that of the wild type.
Co-Authors Andreanus A. Soemardji Marlia Singgih Wibowo TIANA MILANDA TUTUS GUSDINAR Yuana Nurulita