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TUTUS GUSDINAR
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Mutation and Characterization of an Albino Mutant of Monascus sp. Isolated from the Cikapundung River, Bandung TIANA MILANDA; MARLIA SINGGIH WIBOWO; TUTUS GUSDINAR; HARYANTO DHANUTIRTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.286 KB) | DOI: 10.5454/mi.1.1.5

Abstract

Monascus sp. isolated from Cikapundung River, Bandung was mutated using ethyl methanesulfonate (2.5%, 90 min). Previously, this wild type was identified as Monascus purpureus ITBCC-HD-F001 employing random amplification polymorphic DNA (RAPD). Stability of the mutant was observed using color consistency and mutant stability (sub-culturing for five generations) tests. Genetic variation of the mutant (M. purpureus ITBCC-HDF002) was confirmed by RAPD. One of the DNA bands of 1150 bp was found in the albino mutant but not in the wild type, so it was considered as a genetic variation resulting from the mutation process. The albino mutant was characterized by comparing the growth curve, biomass production curve, and the monascidin A production curve of both strains i.e. wild type and the albino mutant. Monascidin A production of the mutant was higher than that of the wild type.
The Antifungal Activity of Artesunate toward Candida albicans: Two Opposite Activities MOHAMMAD RIZKI F ADHIL PRATAMA; DARYONO HADI TJAHJONO; TUTUS GUSDINAR
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1503.088 KB) | DOI: 10.5454/mi.13.3.5

Abstract

The artemisinin and its derivatives antifungal activity continue to be an interesting research object, with the potential shown to be developed as an antifungal compound. Artesunate, one of the artemisinin derivatives known to have antifungal activity against various pathogenic fungi, including Candida albicans. This study aims to determine the effect of artesunate on antifungal activity toward C. albicans in vitro at concentrations below 1 mg -1m L . The method used is yeast-plate count, with a parameter of observation were the number of C. albicans colonies viable after exposure with artesunate for five days. The concentration of artesunate used was divided into -1 -2 -3 -4 -1si x groups, which were 10; 1; 10 ; 10 ; 10 ; and 10 mg mL . Compared to control, a significant decrease in -1c olony counts was only shown at the highest concentration of 10 mg mL . Interestingly, at the lowest -4 -1c oncentration of 10 mgmL , it showed an increase in a number of colonies almost twice of the blank. These results suggest that while at higher concentration of artesunate may inhibit the growth of C. albicans, a lower concentration of artesunate may stimulate their growth.
Co-Authors Daryono Hadi Tjahjono Haryanto Dhanutirto Marlia Singgih Wibowo Mohammad Rizki Fadhil Pratama TIANA MILANDA