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Marlia Singgih Wibowo
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Uji Mekanisme Kerja Antibakteri Senyawa 1,5-difuril-1,4-pentadien-3-on ANALOG Kurkumin terhadap Beberapa Bakteri Dwiningsih Dwiningsih; Vivin Nopiyanti; Ismi Rahmawati; Marlia Singgih Wibowo; Daryono Hadi Tjahjono
Biomedika Vol 9 No 1 (2016): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.946 KB) | DOI: 10.31001/biomedika.v9i1.259

Abstract

Senyawa monokarbonil analog kurkumin yang sudah berhasil disintesis yaitu senyawa 1,5-difuril-1,4-pentadien-3-on, mempunyai aktivitas sebagai antioksidan, anti inflamasi dan antibakteri. Penelitian ini bertujuan untuk mengetahui mekanisme kerja senyawa analog kurkumin 1,5-difuril-1,4-pentadien-3-on terhadap bakteri Klebsiella pneumonia ATCC 10031, Shigella dysenteriae ATCC 9361, Pseudomonas aeruginosaATCC 27853 dan Bacillus subtilis ATCC 6633 yang teraktif. Senyawa 1,5-difuril-1,4-pentadien-3-on merupakan hasil sintesis yang dinyatakan murni dan memiliki hasil elusidasi struktur yang sesuai. Senyawa hasil sintesis diuji aktivitas antibakteri dengan menggunakan metode difusi dengan pelarut DMSO serta kontrol positif amoksilin dilanjutkan dengan metode dilusi. Senyawa 1,5-difuril-1,4-pentadien-3- on dibuat dengan konsentrasi 1500 ppm. Suspensi bakteri disetarakan dengan Mc Farlan 0,5. Hasil aktivitas dengan metode dilusi ditentukan hasil KBM (Konsentrasi Bunuh Minimal) untuk dilakukan kajian mekanisme kerja dengan melihat kebocoran membran dan rusaknya dinding sel bakteri. Hasil menunjukkan senyawa 1,5-difuril-1,4-pentadien-3-on memiliki aktivitas terhadap bakteri K. pneumonia ATCC 10031, S. dysenteriae ATCC 9361, P. aeruginosa ATCC 27853 dan B.subtilis ATCC 6633 dengan diameter daya hambat rata-rata berturutan adalah 16mm; 18,6mm; 21,2mm dan 19mm. Senyawa1,5-difuril-1,4-pentadien-3-on merupakan senyawa teraktif terhadap bakteri P. aeruginosa ATCC 27853. Uji aktivitas antibakteri dilanjutkan dengan metode dilusi untuk mendapatkan nilai KBM (Konsentrasi Bunuh Minimal). Hasil KBM senyawa 1,5-difuril-1,4-pentadien-3-on adalah 187,5 ppm. Uji mekanisme kerja dilakukan dengan pengujian kebocoran membran dan kerusakan dinding sel yang dilihat dengan AAS. Hasil menunjukkan adanya kerusakan dinding sel bakteri karena terjadinya peningkatan konsentrasi Mg2+ pada perlakuan.
KARAKTERISASI TOXOPLASMA GONDII ISOLAT INDONESIA Sagung Chandra Yowani; Endang Kumolosasi; Marlia Singgih Wibowo
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1226.125 KB)

Abstract

Cathodic protection basically reduces the corrosion rate of a metallic structure by reducing its corrosionpotential, Toxoplasma gondii isolated from diaphragm of sheep at an abbatoir in Sukabumi, West Java had beencharacterized by Centre Research Institute for Animal Sciences. The characterization included study of morphologyby optical microscope, study of ultrastructure by transmission electron microscope, the study of the parasite growthin mice Mus musculus, and study of proteins of the parasite. The growth of parasite in mice had been studied usingtwo groups of mice i.e., normal group and immunosuppressed group. The number of parasites was comparedstatistically using student’ t-pair test. Results showed that dexamethasone at a dose of 5.2 mg/20 g body weight intraperitoneally to the immunosuppresed mice did not increase the number of extracelluler parasites in the peritonealfluid. The best parasite harvest time was on the 4th day after inoculation. Determination of parasite protein obtainedat 4 days after inoculation using sodium dodecyl sulphate polyacrylamide gel electrophoresis showed a dominantsurface protein of 42 kDa.
Mutation and Characterization of an Albino Mutant of Monascus sp. Isolated from the Cikapundung River, Bandung TIANA MILANDA; MARLIA SINGGIH WIBOWO; TUTUS GUSDINAR; HARYANTO DHANUTIRTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.286 KB) | DOI: 10.5454/mi.1.1.5

Abstract

Monascus sp. isolated from Cikapundung River, Bandung was mutated using ethyl methanesulfonate (2.5%, 90 min). Previously, this wild type was identified as Monascus purpureus ITBCC-HD-F001 employing random amplification polymorphic DNA (RAPD). Stability of the mutant was observed using color consistency and mutant stability (sub-culturing for five generations) tests. Genetic variation of the mutant (M. purpureus ITBCC-HDF002) was confirmed by RAPD. One of the DNA bands of 1150 bp was found in the albino mutant but not in the wild type, so it was considered as a genetic variation resulting from the mutation process. The albino mutant was characterized by comparing the growth curve, biomass production curve, and the monascidin A production curve of both strains i.e. wild type and the albino mutant. Monascidin A production of the mutant was higher than that of the wild type.
Optimasi Formula Lipid Nanostruktur dengan Pentarget Manosa sebagai Sistem Penghantaran Rifampisin Tri Suciati; Nurani Istiqomah; Benny Permana; Elin Julianti; Marlia Singgih Wibowo; Titah Yudistira; Yani Triyani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1017.477 KB) | DOI: 10.35814/jifi.v17i2.568

Abstract

Limited accumulation of anti-tuberculosis drugs in macrophages become a barrier to the success of latent tuberculosis therapy. The purpose of this study is to develop a D-mannose modified nanoparticle formula as a targeting agent to the mannose receptors to increase the internalization of rifampicin into macrophages. D-mannose was conjugated with chitosan using an amine reducing agent. Chitosan-D-mannose conjugate was characterized using FTIR. Subsequently, the conjugate was adsorbed onto the nanostructured lipid carrier (NLC) electrostatically. The NLC formula consisted of an ethyl acetate solution of solid-liquid lipid blend and rifampicin and an aqueous solution of chitosan-D-mannose conjugate, which were emulsified using polysorbate 80. Solidification of the NLC-chitosan-mannose nanoparticles was carried out by ionotropic gelation and solvent evaporation. The nanoparticle formula was optimized using Box-Behnken design. The formation of chitosan-D-mannose conjugate was shown by the change of the amide band wave number and the Schiff base formation of the FTIR spectra. The optimum formula of nanoparticles had a diameter of 766.1 ± 57.56 nm with a polydispersity index of 0.32 ± 0.02, encapsulation efficiency of 91.54 ± 0.18% and drug loading of 36.62 ± 0.07%. Rifampicin was released from the nanoparticles at pH 5.2 or 7.4 with a similar rate. This D-mannose modified NLC formula has the potential to be further developed as an intracellular antibiotic targeting to macrophages.
Co-Authors Benny Permana Daryono Hadi Tjahjono Dwiningsih Dwiningsih Elin Julianti Endang Kumolosasi Haryanto Dhanutirto Ismi Rahmawati Nurani Istiqomah Sagun Chandra Yowani TIANA MILANDA Titah Yudistira Tri Suciati TUTUS GUSDINAR Vivin Nopiyanti Yani Triyani