Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : BERITA BIOLOGI

PEMANFAATAN BERBAGAI SENYAWA NITRIL DAN PRODUK DEGRADASINYA SEBAGAI SUBSTRAT UNTUK PERTUMBUHAN ISOLAT BAKTERI TP Dyah Supriyati; Bambang Sunarko
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1129

Abstract

Our experiments showed that bacterial isolate TP was able to grow on acetonitrile, butyronitrile and propionitrile as sole sources of carbon, energy and nitrogen, but not on acrylonitrile dan benzonitrile. Besides on nitriles, isolate TP could grow on acetamide,propionamide, benzamide and nicotinamide, but not on acrylamide. However, none of the tested carboxylic acids could be used as growth subtrate for bacterial isolate TP. The best growth substrates of isolate TP were butyronitrile (CH3-CH2-CH2-CN) and propionamide (CH3-CH2-CONH2). When isolate TP grew on butyronitrile, the highest biomass concentration, the doubling-time (td), and the specific growth rate1(n) were 8.99 gram cell dry weight/liter, 4.8 h and 0.144 h , and when grew on propionamide were 4.57 gram cell dry weight/liter, 5.7 h and10.122 h", respectively.
ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Bambang Sunarko; Adityarini Adityarini; Usman Sumo F Tambunan; Nunik Sulistinah
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1151

Abstract

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (\j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (\i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.
PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Nunik Sulistinah; Bambang Sunarko
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1104

Abstract

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(\j was 6 h 40 minutes, the specific growth rate (\J) was 0,046 h'\ the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.