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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Annales Bogorienses
Ahmad Thontowi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Isolasi dan Karakterisasi Mikrob Pendegradasi Senyawa Nitril Ahmad Thontowi; Bambang Sunarko
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.87

Abstract

Biotransformation of nitrile compound using microbe represents the best way in eliminating  toxicity. Besides, microbes and enzymes of nitriles degradation can be used in synthesis of various chemical compounds with a high economic value. The purpose of this research is to obtain microbial potential for nitriles degradation, to obtain information of physiological of chosen microbe and the characteristic of its enzyme. We have conducted are isolation and selection of microbial nitriles degradation, perception of growth pattern, determination of influence various and concentration of nitriles on microbial growth, determination of enzymes specificity and inductivity, and determination of optimum pH and temperature. The result of microbial isolation from various sources of waste obtained by 12 isolates at acetonitrile, 18 isolates at benzonitrile, 8 isolates at adiponitrile, 1 isolate at lactonitrile, and 1 isolate at ß- aminopropionitrile. GLB5 isolate was selected from 46 isolates, because this isolate can grow at some tested nitriles. The best growth of GLB5 isolate in acetonitrile 360 mM, benzonitrile 30 mM, 3-sianopiridin 15 mM, and ß- aminopropionitrile 140 mM. The growth of GLB5 isolate in acetonitrile 360 mM were experienced a lag phase during 8 hours, and an exponential phase during 40 hours. Time of cell doubling during 7 hours and 42 minutes, and specific growth rate (µ) equal to 0,09 h-1. The character of nitriles degradation enzymes from GLB5 whole cell was inductive, with optimum activity at pH and temperature equal to 7.2 and 40oC.
Alkane Degradation and Detection of Mono-xygenase Gene from Alcanivorax sp. from Jakarta Bay Ahmad Thontowi; Yopi Yopi
Annales Bogorienses Vol. 15 No. 2 (2011): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2011.v15.n2.25-30

Abstract

Alkanes is a major component of crude oil that can be hydrolized by enzyme alkane monooxygenase from bacteria. Nine oil-degrading bacteria were analyzed their capability to degrade alkanes (pristane and paraffin). The result of growth test on paraffin and pristane were showed that 9 isolates could be divided into two groups. First group (BL09, BL31 and BL45) could degrade both paraffin and pristane, and second group (BL01, BL06, BL44, BL057, BL058 and BL071) preferred to degrade paraffin than pristane. Three isolates (BL09, BL31 and BL45) have activity to decrease paraffin and pristane until less 50% remain. Based on homology analysis of 16SrRNA gene sequences showed that isolates No. BL09, BL31 and BL45 were identified as Alcanivorax sp. and the partial sequences of the alkB gene from those three isolates are showing 66-68% of identity compare with some mono-oxygenase gen from database of genbank.
Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Ahmad Thontowi; Nanik Rahmani; Yopi Yopi
Annales Bogorienses Vol. 17 No. 1 (2013): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2013.v17.n1.33-42

Abstract

Bto5 is marine bacterium
Co-Authors Bambang Sunarko Nanik Rahmani Nanik Rahmani Yopi Yopi Yopi Yopi