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PAT-2 Rapid Diagnostic Test of Red Sea Bream Iridoviral Disease (RSIVD) in Grouper Epinephelus sp. Based on Serological Co-Agglutination and Molecular Study Dwi Sulistiyono; Surya Amanu; Kurniasih Kurniasih; Yuli Purwandari Kristianingrum
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.403 KB)

Red sea bream iridoviral disease (RSIVD) is caused by red sea bream iridovirus (RSIV), adouble stranded DNA of Icosahedral virus with a diameter of 120-240 nm [1]. RSIV is one of the species of the Megalocytivirus, Genus of the Iridoviridae Family, first reported to infect red sea bream (Pagrus major) fish, at Sikoku Island, Japan 1991, and since then it has been noted to cause considerable economic losses to fisheries in Singapore, Taiwan, Thailand, Korea, Philippines, Malaysia and also in Indonesia [2,3,4]. Rapid transmission with high mortality rates in fish populations infected becomes a serious threat to the aquaculture fishery business. Stained imprints or tissue sections [1], monoclonal antibody technique (MAb), Immunofluorescent Antibody Tests (IFAT) [5], Polymerase Chain Reaction (PCR) [6] Electron Microscope and Multiplex PCR [2] methods have been introduced. Although it is very effective for detecting RSIVD in infected fish, but requires training and specialized equipment at a high cost.Co-agglutination test is a diagnostic method, used both in humans and animals in detecting bacterial or viral diseases [7], this method is fast, easy to use, and does not require special equipment. Test results from co-agglutination are easily seen macroscopically, so it is suitable if developed in RSIVD detection in the field case. This study aims to create and conduct RSIVD co-agglutination kit field tests supported by molecular studies and diagnostic analysis of the sensitivity and specificity of the accuracy and reliability of the kit. Then the test results will be compared from the pooling and individual samples.

Lesi Patologik Organ dan Jaringan Ikan Nila (Oerochromis niloticus) yang Diinfeksi Bakteri Staphylococcus sp. Bambang Sutrisno; Yuli Purwandari K.
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Lesi Patologik Organ Dan Jaringan Ikan Nila (Oerochromis nilaticus) Yang Diinfeksi Bakteri Staphylococcus sp. Bambang Sutrisno; Yuli Purwandari K.
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer Yuli Purwandari Kristianingrum; Sitarina Widyarini Sitarina Widyarini; Kurniasih Kurniasih; Bambang Sutrisno Bambang Sutrisno; Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.

Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer Yuli Purwandari Kristianingrum; Sitarina Widyarini Sitarina Widyarini; Kurniasih Kurniasih; Bambang Sutrisno Bambang Sutrisno; Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.

Immunodiagnosis Infeksi Aeromonas hydrophila pada Ikan Yuli Purwandari Kristianingrum; Sitarina Widyarini; Kurniasih Kurniasih; Bambang Sutrisno; Charles Rangga Tabbu; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Aeromonas hydrophila causes a disease that often infects fish and is known as Motile Aeromonas Septicaemia (MAS), Hemorrhagi Septisemia, Ulcer disease or Red-Sore disease. The aims of this study were to develop polyclonal antibody of Aeromonas hydrophila in the rabbits to confirm the diagnosis of Aeromonas hydrophila in the fish by immunohistochemistry staining method. Preparation of polyclonal antibodies was performed on the rabbits used to Aeromonas hydrophila bacteria that have been tested biochemically by intravenous and intraperitoneal injection. Doses of Aeromonas hydrophila bacteria were 109 CPU/ml of 0.5 ml at first injection, 1 ml at second injection, 2 ml at thirth injection and 3 ml at fourth injection. Blood serum collection was performed at week 5 after injection from an ear and intracardial vein. The result of antibody titer was 28 = 1024 which measured by tube test. Furthermore, polyclonal antibody was used to immunohistochemistry staining with 400x dilution. The results of the staining showed that an immunopositive reaction in the liver, skin,lien, gill, kidney, and heart of fish to Aeromonas hydrophila antibody. The research conclution was polyclonal antibody from rabbit can be used to accurately confirm the diagnosis of Aeromonas hydrophila based on antigen and antibody reaction.

Studi In-Vivo Ekstrak Daun Teh Hijau (Camellia Sinensis) sebagai Alternatif anti Bakteri Eschericia Coli pada Ayam Broiler Bambang Sutrisno; R. Wasito; Kurniasih Kurniasih; Sitarina Widyarini; Yuli Purwandari Kristianingrum; Sugiyono Sugiyono
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The prevalence of colibasillosis in chicken farms in Indonesia is very high, treatment using antibiotics is experiencing resistance, so it is necessary to look for alternatives to antibacterial. The study was aimed to determine the antibacterial effect of green tea leaf extract on broiler chickens infected with Eschericia coli by looking at the score of macroscopic lesions strengthened by histopathological examination, heterophile examination, plasma protein and fibrinogen. The research used 20 day old broilers (DOC) which were randomly divided into 4 groups, group A group B, group C and group D, each consisting of 5 DOC broilers. While maintaining ND and Gumboro vaccines on schedule like maintenance in general. At the age of 21 days all broilers in each group began to be treated as controls (Group A) without infecting E. coli and were not given 0,1g/ml water extract of green tea leaves (Camillia sinensis). Group B, intratracheal-infected broilers with local strains of E.coli were 108 cells / ml according to 0,5 Mc Farland standard, and were not given green tea leaf extract. Group C, broilers infected by intratracheal with local strains of E. coli 108 cells / ml by 0,5 Mc Farland standard, and given to drink green tea leaf extract (Camillia sinensis) 0,1 g/ml and group D, broilers were given drinking green tea leaf extract (Camillia sinensis) 0,1g/ml. During the treatment all of chickens were given food and drink ad libitum. Fourteen days after infection of E.coli, 5 chickens in each group were collected to collect blood for heterophyll, total plasma protein (TPP) and fibrinogen. And then were euthanasied with Mg SO4 saturated solution intravenously injection and necropsied for gross and histpathological examination. Analysis of blood tests results were used one way of anova (SPSS version 22 program), whereas for gross and histopathological examination with descriptive analysis. The results showed that the gross examination and histopathological organs of brolier infected with E. coli without being given a green tea extract experienced airsacculitis, pericarditis, perihepatitis and peritonitis, whereas broilers infected with E. coli and given green tea extract does not indicate the presence of inflammation. Examination of heterophile counts and blood fibrinogen levels had shown a difference (P <0.05), in broilers infected with E. coli and given green tea extracts had lower amounts of hetrophils and fibrinogen levels. While blood TPP levels were not significantly different (P> 0.05). The conclusion can be drawn, that the study of in vivo green tea extract (Camelia sinensis) 0,1g/ml has the potential to inhibit the infection of Eschericia coli bacteria in broiler chickens.

Gangguan Pertumbuhan Organ Limfoid Ayam Broiler yang Menderita Omfalitis Bambang Sutrisno; R Wasito; Sitarina Widyarini; Yuli Purwandari Kristianingrum; Sugiyono .
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60465

Penelitian ini bertujuan untuk melihat gangguan pertumbuhan jaringan limfoid primer dan sekunder yang menderita omphalitis dengan pemeriksaan histopatologi diwarnai dengan pewarnaan hematoksilin-eosin rutin dan pewarnaan imunohistokimia streptavidin biotin terhadap interleukin-10 (IL-10) pada ayam muda. Ayam umur 24 hari (DOC) broiler digunakan dan dikumpulkan dari tempat penetasan yang sama di Jawa Tengah di Indonesia. Semua 24 DOC dibagi menjadi dua kelompok yang masing-masing terdiri dari 12 DOC (Grup A) dan 12 DOC omphalitic (Grup B). Semua DOC dirawat di kandang yang berbeda, diberi makan dan diminum di libitum. Pada hari ke 3, 6 dan 9, empat ekor ayam dari masing-masing kelompok ditimbang untuk kemudian dinekropsi. Timus, bursa fabricius dan limpa dikumpulkan dan ditimbang. Semua jaringan diproses secara histopatologi dengan pewarnaan hematoksilin-eosin rutin dan pewarnaan biotin streptavidin imunohistokimia imunopatologi. Data indeks berat limpa, bursa Fabricius dan tymus dianalisis menggunakan program statistik IBM SPSS versi 22. Hasil penelitian menunjukkan bahwa indeks bobot limpa, bursa fabricius dan timus ayam omphalitic (kelompok B) lebih rendah dibandingkan dengan indeks bobot ayam sehat (kelompok A). Indeks berat timus berbeda nyata (P <0, 05). Lesi histopatologis pada organ limfoid diamati pada semua ayam di Grup B. Lesi ditandai dengan penipisan dan nekrosis limfosit. Ayam dari Grup A tidak mengalami perubahan pada organ limfoid. Biotin streptavidin immunostaining dengan ekspresi antibodi policlonal anti IL-10 pada bursa Fabricius pada ayam omphalitic (Grup B) memiliki IL-10 yang sangat sedikit jika dibandingkan dengan ayam sehat (Grup A). Kesimpulan, omfalitis menyebabkan penurunan indeks berat badan yang signifikan dan gangguan pertumbuhan organ limfoid yang ditandai dengan deplesi dan nekrosis limfosit.

STUDI HISTOPATOLOGIS TUMOR PADA HEWAN KESAYANGAN AND WILD ANIMAL Sitarina Widyarini; Sugiyono Sultan; Yuli Purwandari Kristiangingrum; Bambang Sutrisno
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70506

Penelitian ini dilakukan untuk mengamati gambaran histomorfologis (berdasarkan pada histogenesis dan sifat pertumbuhan tumor), pada berbagai sampel tumor dari hewan kesayangan (anjing dan kucing) dan wild animals (beruang) koleksi Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada. Duapuluh satu sampel (22) dari organ yang diduga jaringan tumor yang diperoleh dari Dokter Hewan Praktisi maupun Rumah Sakit Hewan selama tahun 2019 digunakan dalam penelitian ini. Semua sampel tersimpan dalam kontener berisi larutan formalin 10%. Selanjutnya sampel diproses dan diwarnai dengan Hematoxylin-Eosin. Pengamatan terhadap histogenesis dan sifat pertumbuhan tumor dilakulan dengan menggunakan mikroskop cahaya dengam pembesaran 200X dan 400X dan selanjutnya dilakukan dokumentasi dengan pengambilan foto. Analisis dilakukan secara deskriptif dengan membandingan dengan referensi histomofologis tumor pada hewan. Hasil penelitian memperlihatkan bahwa selama tahun 2019, jumlah sampel tumor kulit dilaporkan paling banyak 63.63% (14/22), diikuti dengan jumlah sampel tumor pada kelenjar mammae 22,72% (5/22), liver-duktus biliverus 18.18% (4/22), tulang 4,54% (1/22) dan metastasis paru-paru dan metastasis paru-paru dari jaringan tulang sejumlah 4,54% (1/22). Hewan asal tumor paling banyak ditemukan pada kucing dan anjing masing-masing 59% dan 34%. Hasil pengamatan terhadap gambaran histomorfologis ditemukan adenokarsinoma mammae (tubulopapillary carsinoma, solid adenocarsinoma, lipid-rich adenokarsinoma), fibroadenomatous hiperplasia mammae, karsinoma sel skuamosa, papiloma, adenoma kelenjar hepatoid, melanositosis, fibrosarkoma, kolangiokarsinoma dan osteosarkoma.

Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.278 KB)

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.

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