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The Distribution of MAP-2 Phosphorylation in Cerebral Cortex of Long-Tailed Monkey Fetuses (Macaca fascicularis) in the Last Trimester of Gestation Pangestiningsih, Tri Wahyu; Irawan, Vidya; Sulistiawati, Erni
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.706 KB)

Memories are storage in cholinoceptive cells, the cells which are enriched with microtubule-associated protein 2 (MAP-2) that localized in the neuronal dendrite and the cell bodies. Phosphorylation of MAP-2 may increase memory with reduce stability of dendrite by altered dendrite length and lead new side-branches of neuronal as a neuronal plasticity processes in cerebral cortex. The aim of this research is to study the distribution of MAP-2 phosphorylation neurons in cerebral cortex of long-tailed macaques in the third semester of gestationalimmunohistochemically using avidin biotin conjugated complex method. Neurons MAP-2 phosphorylation immunoreactive were located in dendrites and cell bodies, mostly in pyramidal neurons of cerebral cortex. Intensity of MAP-2 phosphorylation immunoreactivity in layer V were stronger than another layer and the neurons that very intensely stained were the pyramidal cells in frontal and parietal lobes, that was suggested that neurons in this areas more responsive to neuroplasticity. From the results we concluded that MAP-2 phosphorylation already distributed in the cerebral cortex of long-tailed macaque fetuses at the last trimester of gestation, mostly in the pyramidal cells of layer V that is suggested plays a role for preparation of memoryformation.Keywords: fetus, long-tailed monkey, cerebral cortex, memory, MAP-2 phosphorylation

Serum and urinary neutrophil gelatinase-associated lipocalin as a predictor of rat kidney histopathology in an early ischemia-reperfusion model Panggabean, Sahala; Sulistiawati, Erni; Chairani, Nuryati; Mansyur, Muchtaruddin; Susalit, Endang
Medical Journal of Indonesia Vol 21, No 4 (2012): November
Publisher : Faculty of Medicine Universitas Indonesia

Background: The severity of ischemia-reperfusion (I/R) kidney injury is highly correlated with mortality and morbidity rate. Research on human and animal prove that NGAL predicts kidney injury at early phase. The objective of this study is to prove that the increase in serum and urinary NGAL are correlated with kidney tubular epithelial damage, and this increase has occurred in initiation phase, indicated by rat kidney histopathology in an early I/R model.Methods: Twenty eight male Sprague-Dawley rats were divided into 4 groups: 4 hour sham (Sham 4), 8 hour sham (Sham 8), 10 minute ischemia 4 hour reperfusion (I/R 4) and 10 minute ischemia 8 hour reperfusion (I/R 8). Blood, urine and kidney samples were collected. Serum creatinine level was analyzed with Jaffe method, while serum and urinary NGAL level were analyzed with direct sandwich ELISA method. Evaluation of kidney damage were measured semi quantitatively in tissue stained with HE. Further evaluation to confirm cellular changes on kidney was performed by electron microscope and immunohistochemistry.Results: Serum NGAL was found significantly correlated with degree of kidney tissue damage (ρSpearman NGAL serum = 0.701, p < 0.001), also urinary NGAL (ρSpearman = 0.689, p < 0.001). NGAL expression differs significantly between I/R group and sham (t-test, t = -26635.056, p < 0.001), also kidney damage (t-test, t = -5.028, p < 0.001), and serum and urinary NGAL levels (Mann-Whitney, U = 0, p < 0.001). With cutoff points of 136.95 ng/mL and 58.69 ng/mL subsequently for serum and urinary NGAL , it is found that sensitivity = 1, specificity = 1.Conclusion: Elevation of serum and urinary NGAL are significantly correlated with epithelial tubular kidney damage on rat undergoing early ischaemia reperfusion. (Med J Indones. 2012;21:208-13)Keywords: Early I/R kidney injury, kidney histopathology, NGAL

Efektivitas Ivermectin dan Albendazole dalam Melawan Ostertagia pada Anakan Domba di Bogor, Indonesia Silvia Puspitasari; Erni Sulistiawati; Muladno Basar; Achmad Farajallah
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 3 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Giving a half-dose of an anthelmintic is a simple method for detecting resistance in parasites infesting small ruminants. When a single anthelmintic fails in native sheep from Indonesia, a combination of anthelmintics from different chemical classes with different mechanism of action is an alternative parasite-control strategy. This study compared the anthelmintic effectiveness of ivermectin (IVM) and albendazole (ABZ) have given either separately as a full-dose or half-dose or co-administered to sheep naturally infected with Ostertagia. Twelve sheep from Bogor, Indonesia were divided into six groups based on the anthelmintic treatment: half-dose IVM, full-dose IVM, half-dose ABZ, full-dose ABZ, combined (half-dose IVM + half-dose ABZ), and control groups. The treatment effectiveness was determined using the Faecal egg count reduction (FECR) on before and after 7, 14, 21, 28, 35, and 42 days of treatment. FECR of half-dose IVM, full-dose IVM, half-dose ABZ, full-dose ABZ, and both combined ranged from 78-100%, 96-100%, -71-89%, -521-64%, and 54-100%, respectively. The results showed that full-dose IVM was the most effective to against Ostertagia until 42 days and followed the combined that effective until 28 days. Half-dose and full-dose ABZ weren’t effective to against Ostertagia.

Komparasi Data Hematologi pada Beberapa Spesies Macaca Erni Sulistiawati; Luluk Lailatul Hasanah; Dondin Sajuthi; Yohana Tri Hastuti
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.31-38

Satwa primata merupakan salah satu hewan model yang banyak digunakan dalam penelitian biomedis karena secara anatomi dan fisiologis memiliki kesamaan dengan manusia. Darah merupakan salah satu cairan tubuh ekstraseluler yang berfungsi sebagai alat angkut atau media transport dan pertahanan tubuh. Gangguan keseimbangan yang terdapat pada setiap variabel kuantitatif dan kualitatif dari jenis sel-sel darah dapat menyebabkan validitas suatu penelitian menurun. Penelitian ini bertujuan untuk mendapatkan data dasar hematologi Macaca siberu dan untuk mendapatkan informasi kekerabatan melalui data sekunder hematologi secara kuantitatif dan kualitatif dari Macaca leonina, dan Macaca nemestrina. Sampel diambil dari 8 ekor Macaca siberu (5 jantan dan 3 betina) dengan umur yang bervariasi dan tergolong sehat serta telah dipuasakan. Analisis sampel yang dilakukan yaitu berupa pemeriksaan diferensial leukosit, pemeriksaan kuantitatif sel-sel darah, dan indeks eritrosit yang dianalisis secara deskriptif. Profil hematologi pada Macaca siberu secara keseluruhan menunjukkan hasil yang tidak jauh berbeda dengan Macaca nemestrina dan Macaca leonina. Variasi nilai yang ditemukan dapat dipengaruhi oleh faktor intrinsik seperti jenis kelamin, nutrisi, aktivitas dan stres, serta faktor ekstrinsik seperti suhu, ketinggian, dan letak geografis.

Carbohydrate is a diverse compound in the body and can be as glycoconjugate which bound to protein in proteoglycan and glycoprotein shape, and bound to lipid in glycolipid shape as well. The study was conducted to get information image of pattern distribution of the glycoprotein at stomach gland from various age levels at Macaca fascicularis (Mf) after and before bearing. Gastric specimen was collected processed through microtechnique procedure, followed by histochemistry Lectin staining to det Erdiansyah Rahmi; Dondin Sajuthi; Srihadi Agungpriyono; Erni Sulistiawati
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Carbohydrate is a diverse compound in the body and can be as glycoconjugate which bound to protein in proteoglycan and glycoprotein shape, and bound to lipid in glycolipid shape as well. The study was conducted to get information image of pattern distribution of the glycoprotein at stomach gland from various age levels at Macaca fascicularis (Mf) after and before bearing. Gastric specimen was collected processed through microtechnique procedure, followed by histochemistry Lectin staining to detect glycoprotein from stomach. Observation result of given score subjectively into four categories: (-) none/negatif, (+) low, (++) medium,and (+++) height. The results obtained were tabulated and analyzed diskriptively. We found that glycoproteins complex exist in the glandullar stomach of long tailed macaques are mannose, glucose, galactose, N-acetil-D-glocosamine and N-acetil-D-galactose all of these glycoproteins complex was observed at age foetus 70 days. Glycoprotein can act as energy resource used for grouht and protections toward stomach. Keywords: glycoprotein, lectin, stomach gland, Macaca fascicularis

Intervensi Nikotin terhadap Level Low Density Lipoprotein, dan Ekspresi UCP-1 (Uncoupling Protein 1) pada Monyet Ekor Panjang (Macaca fascicularis) Obes dengan Resiko Aterogenesis Anwar Wardy Warongan; Irawan Jusuf; Dondin Sajuthi; Erni Sulistiawati; Sri Supraptini Mansjoer; Ria Oktarina
Jurnal Primatologi Indonesia Vol. 7 No. 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstrak: Lima belas monyet ekor panjang obes (Macaca fascicularis) dengan resiko aterogenesis digunakan untuk memperoleh informasi tingkat low density lipoprotein (LDL) dan ekspresi protein uncoupling-1 (UCP-1) setelah tiga bulan intervensi nikotin dosis rendah per oral. Kelompok hewan dibagi menjadi tiga dengan komponen diet berbeda, yaitu diet A (lemak), diet tipe B (lemak dan kuning telur), dan diet tipe C (komersial diet-monyet chow), dan setiap kelompok terdiri dari lima monyet. Ketiga diet tersebut dicampur nikotin dan diberikan ke dalam makanan sehari dengan dosis 0,50-0,75 mg/kg/12 jam. Pengamatan terhadap lipid darah dilakukan setiap bulan dan pada saat nekropsi diambil jaringan adiposa coklat untuk evaluasi dengan teknik fluoresensi antibodi. Berdasarkan analisis lipid menunjukkan bahwa ada peningkatan yang signifikan konsentrasi LDL (p

KIVFA-3 Analisis dan Kajian Kadar Logam dalam Darah Sapi Potong Erni Sulistiawati; Cynthia Novita; Puji Rahayu
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Beberapa pemilik hewan ternak menggembalakan hewan ternaknya di Tempat Pembuangan Akhir (TPA). Pemeliharaan seperti ini dilakukan karena keterbatasan ladang penggembalaan dan pertumbuhan rumput yang tidak sebanding dengan kebutuhan pakan sapi. Masuknya mineral logam berbahaya ini tidak hanya melalui proses memakan, melainkan dapat juga melalui air minum atau kebiasaan ternak yang suka menjilat segala benda yang terdapat di TPA. Pakan sapi yang tercemari oleh berbagai bahan cemaran seperti material logam akan menimbulkan kerugian yang memang tidak secara langsung membunuh ternak, namun meterial logam berbahaya tersebut dengan kadar tinggi khususnya timbal (Pb) dan kadmium (Cd) dapat membahayakan kesehatan sapi dan munusia yang mengkonsumsi daging sapi potong tersebut (3)(10)(11). Selain itu akumulasi logam lainnya yang berlebih pada daging sapi dapat juga sejumlah tanda klinis diare, kelemahan otot dan gangguan fungsi ginjal (1) dan kanker.Tujuan penulisan ilmiah ini adalah mendapatkan informasi analisis kandungan berbagai jenis material logam seperti timbal (Pb), cadmium (Cd), cobalt (Co), cuprum (Cu), Zink (Zn) dan Magnesium (Mg) dalam darah sapi potong dan mengkaji dampak kandungannya pada tubuh sapi potong.

KIVSA-7 Persentase Kejadian Hiperbilirubinemia pada Kucing Zulfitra Utami Putri; Dondin Sajuthi; Erni Sulistiawati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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PENDAHULUANHiperbilirubinemia merupakan kondisi patologis akibat terjadinya peningkatan kadar bilirubin di dalam darah (1). Hiperbilirubinemia ditandai dengan temuan klinis berupa ikterus. Ikterus dapat dikategorikan menjadi tiga bentuk yaitu prehepatik, hepatik dan posthepatik.Ikterus prehepatik dapat disebabkan oleh meningkatnya bilirubin indirek di dalam darah yang dapat terjadi akibat adanya destruksi sel darah merah secara berlebihan (hemolisis). Kondisi ini pada kucing dapat disebabkan oleh berbagai penyebab seperti immune mediated hemolytic anemia (IMHA), perubahan sekunder dari penyakit infeksius seperti feline infectious peritonitis (FIP), feline leukemia virus (FeLV), dan infeksi Mycoplasma haemofelis (5). Ikterus prehepatik tidak hanya terkait dengan adanya hemolisis namun juga dipengaruhi oleh kapasitas organ hati dalam mengkonjugasi bilirubin yang terkait dengan hypoxic liver damage yang umumnya terjadi bersamaan dengan anemia (8).Ikterus hepatik merupakan adanya akumulasi bilirubin indirek atau direk akibat adanya kerusakan atau gangguan pada organ hati. Kondisi ini dapat disebabkan oleh hepatik lipidosis, kolangitis, toksik hepatopati dan hepatik neoplasia (5). Sedangkan, ikterus posthepatik yaitu adanya akumulasi bilirubin direk akibat gangguan traktus hepatobiliari atau obstruksi saluran empedu. Yangmana pada kucing umumnya terkait dengan feline triaditis syndrome dan liver fluke (2)(7).Hiperbilirubinemia pada kucing kurang sensitif jika digunakan sebagai indikator gangguan pada hati karena kurang dari 50% kejadian kasus gangguan fungsi hati pada kucing menunjukkan adanya hiperbilirubinemia yang disertai dengan ikterus (6). Gangguan fungsi hati berkorelasi dengan adanya kondisi anemia. Anemia dapat terjadi selama adanya gangguan fungsi hati setelah terjadinya infeksi, perdarahan atau neoplasia (4).Penulisan ilmiah ini bertujuan untuk mendapatkan informasi kejadian hiperbilirubinemia pada kucing melalui penelusuran lebih lanjut terkait ada atau tidaknya anemia dan mengkaji berbagai kausa yang mempengaruhi.

The Distribution of MAP-2 Phosphorylation in Cerebral Cortex of Long-Tailed Monkey Fetuses (Macaca fascicularis) in the Last Trimester of Gestation Tri Wahyu Pangestiningsih; Vidya Irawan; Erni Sulistiawati
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Memories are storage in cholinoceptive cells, the cells which are enriched with microtubule-associated protein 2 (MAP-2) that localized in the neuronal dendrite and the cell bodies. Phosphorylation of MAP-2 may increase memory with reduce stability of dendrite by altered dendrite length and lead new side-branches of neuronal as a neuronal plasticity processes in cerebral cortex. The aim of this research is to study the distribution of MAP-2 phosphorylation neurons in cerebral cortex of long-tailed macaques in the third semester of gestationalimmunohistochemically using avidin biotin conjugated complex method. Neurons MAP-2 phosphorylation immunoreactive were located in dendrites and cell bodies, mostly in pyramidal neurons of cerebral cortex. Intensity of MAP-2 phosphorylation immunoreactivity in layer V were stronger than another layer and the neurons that very intensely stained were the pyramidal cells in frontal and parietal lobes, that was suggested that neurons in this areas more responsive to neuroplasticity. From the results we concluded that MAP-2 phosphorylation already distributed in the cerebral cortex of long-tailed macaque fetuses at the last trimester of gestation, mostly in the pyramidal cells of layer V that is suggested plays a role for preparation of memoryformation.Keywords: fetus, long-tailed monkey, cerebral cortex, memory, MAP-2 phosphorylation

Study of Struvite Crystals and Dietary at Risk for the Formation of Struvite Urolithiasis in Dogs in Small Animal Practices Erni Sulistiawati Sulistio
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66464

Urolithiasis is defined as the formation of urolith or crystal in the urinary tract caused by supersaturation of urine. This study was conducted at PDHB drh Cucu Kartini S, dkk, Jakarta clinic. The data were taken from urinalysis test results of 23 dogs which suffered crystaluria and urolith stone examination of 11 dogs which suffered urolith stone post cystotomy. Most affected breeds were Shihtzu, Pug, Mini Pom, Corgi, Miniature Schnauzer, Golden Retriever. Crystaluria most detected in male dogs, while urolith stone most affected in female dogs. Dogs from 6 months to 12.5 years with a median of 5 years were affected. The urinalysis showed that dogs with crystaluria had alkaline urine (mean 8.3), 100% proteinuria, 86.9% hematuria, 41.4% pyuria. Diets is the most contributing factor in case of struvite urolithiasis, which is low water content; high in protein, magnesium, phosphorus, carbohydrates and calcium. Therapeutic diets that low in protein, phosphorus, magnesium and sufficient carbohydrates and diets with greater than 70% moisture can be an option for treatment and prevention of recurrent of struvite urolithiasis

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