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All Journal Saintifika Pelita Perkebunan UNEJ e-Proceeding The Indonesian Biomedical Journal Health Notions BIOEDUKASI Multidiciplinary Journal Pancaran Pendidikan Eduvest - Journal of Universal Studies Jurnal Kesehatan dr. Soebandi

Dwi Wahyuni

Biology Education Study Program, Department Of Mathematic And Nature Education, Faculty Of Teacher Training And Education, Universitas Jember, Jl. Kalimantan Tegalboto No.37, Jember

Author-ID : 2535405

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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Cleaning Officers’ Behavior in Waste Management According to The Standards of Accreditation in X Jember Hospital Carri Noer Fida Yanik; Dwi Wahyuni; Dewi Rokhmah
Health Notions Vol 3, No 1 (2019): January
Publisher : Humanistic Network for Science and Technology (HNST)

Waste management is very important especially in hospitals. Hospital waste management must be done properly and correctly so that it can be prevented from diseases caused by waste that will be felt by all residents of the hospital. The purpose of this study was to analyze factors related to the behavior of cleaning officers in waste management in X Jember Hospital. The research design used was cross sectional. The results showed that age, education, years of service, knowledge and supervision were related to the behavior of cleaning officers in waste management with p value of

Analytical and Phytochemical Exploration of Bioinsecticide Granules Mixed Betel Leaf Extract (Piper betle) and Srikaya Seed Extract (Annona squamosa) Dhina Ayu Susanti; Ady Soesetijo; Dwi Wahyuni
Health Notions Vol 2, No 2 (2018): February
Publisher : Humanistic Network for Science and Technology (HNST)

One alternative in controlling Aedes aegypti larvae is with the use of bioinsecticides that are environmentally friendly, safe, and inexpensive. Betel leaf and srikaya seeds contain chemical compounds that can be used as bioinsectiside. The following paper deals with detection of phytochemical in the Granules Mixed Betel Leaf Extract and Srikaya Seed using Thin Layer Chromatography (TLC) and FTIR. The TLC analysis was performed Rf value for 0.91 flavonoid compound; alkaloids 0.49; saponins 0.29 and 0.65; tannin 0.89; and anthraquinone 0.22 and 0.45. The FTIR analysis was performed represented the presence of various functional groups which includes alcohol, alkana, nitrile, and ether.Keywords: Phytochemical, Bioinsecticide, Granula, TLC, FTIR

POTENSI ANTAGONIS BAKTERIA SERRATIA MARCESSCENS TERHADAP BAKTERI STREPTOCOCCUS PYOGENESIS DAN BAKTERI VIBRIO CHOLERA SECARA IN VITRO Muhammad Efendi; Dwi Wahyuni; Imam Mudakir
Saintifika Vol 18 No 2 (2016)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

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Abstract: In indonesia, infection diseases are a very high problem among the public, one the common infectious diseases suffered by the Indonesian community is imflamtion of the tonsils caused by Streptococcus pyogenesis and Cholera caused by Vibrio cholera. The use of chemical antibiotics will have a negative impact in health, so necessary alternative replecement of natural antibiotics by Serratia mercescens can pruduce prodigiosin. This reseacrh was to test the potential of Serratia mercescens antagonist against Streptococcus phyogenesis and Vibrio cholera in vitro, using Completely Randomized Design with one treatment factor from Serratia mercescens bacteria concentration. The concentration used was 30μm for each bacterial suspension and added positive control in the form of chloramphenicol and negative control of aquades, each of which was repeated 3 repititions. The results were analyzed using one-way ANOVA and continued D- test. The result showed that Serratia mercescens had blocked inhibitability of Streptococcus phyogenesis bacteria with an average resistance of1.1033mm and on Vibrio cholera with an average resistance of 1.2233mm. Keywords: Disease Infections; Bacterial Antagonist; Secondary Metabolism

PERBEDAAN TOKSISITAS EKSTRAK DAUN SIRIH (Piper betle L.) DENGAN EKSTRAK BIJI SRIKAYA (Annona squamosa L.) TERHADAP LARVA NYAMUK Aedes aegypti L. Dwi Wahyuni; Intania Loren
Saintifika Vol 17 No 1 (2015)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.439 KB)

Dengue Fever is a public health problem that is more widespread endemic area for morbidity is high enough into something that must be addressed immediately.The way to solve this desease chemically can make this mosquito becomes resistant. To solve this problem, it needs to find an alternative way in which it can be a natural insecticide that is safe like compounds that comes from Piper betle leaves and Annona squamosa seed. The active compounds of Piper betle leaves that are mixed with the active compounds of Annona squamosa seeds can be used as bioinsecticide. Piper betle leaves contain the active compounds of saponin, alkaloid, flavonoid and tannin, while Annona squamosa seeds contain theactive compounds of annonacin, squamocin, asimisin and alkaloid. This research aimsed to investigate the toxicity of themixture of the Piper betle leaves extract and Annona squamosa seeds againet Aedes aegypti mosquito larvae and tocompare the toxicity of the extract mixture and single extract. The method of this research used complete random design bythree repetitions. The concentratios used were 300, 600, 900, 1200 and 1500 ppm for Piper betle leaves extract and 5, 10, 50, 70 and 100 ppm for Annona squamosa seeds extract with aquades asthe control. The data were analyzed by using probit analysis to determine the LC50. The LC50 score of the Piper betle leaves extract was 910,38 ppm and the LC50 of the Annona squamosa seeds extract was 20,62 ppm. This result showed that toxicity of Piper betle leaves extract with different Annona squamosa seeds which was toxicity is higher than the toxicity of Piper betle leaves extract.

THE TOXICITY OF SEEDS EXTRACT OF ANNONA SQUAMOSA L., LEAVES EXTRACT OF TERMINALIA CATAPPA L. AND LEAVES EXTRACT OF ACACIA NILOTICA L. ON THE MORTALITY OF AEDES AEGYPTI L. LARVAE Dwi Wahyuni; Sandy Pradipta; Muhammad Ramadhan
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Aedes aegypti L. is a species of mosquito that carries the dengue virus causes dengue fever. The way to solve the disease is still focused on synthesis larvacides which have a negative impact on the environment, it needs to be replaced with natural larvicides that are environmentally friendly. Annona squamosa L. seeds extract contains annonain and squomacin. Terminalia catappa L. leaf extract contains quinone, saponin, flavonoid and tannin. Acacia nilotica L. leaf extract contains terpenoid, saponin, flavonoid and tannin. This research aims to determine The toxicity of seeds extract of A. Squamosa, leaves extract of T. Catappa, and Leaves extract of A. nilotica on the mortality of Ae. aegypti. The method used in this research was complete randomized design using four replications. Concentration used 5, 10, 50, 100, 150, 200 and 250 ppm for the seeds extract of A. Squamosa, 50, 100, 150, 200, 250, 300 and 350 ppm for the leaf extract of T. catappa, and 80, 120, 180, 240, 300, 350 and 400 ppm for the leaf extract of A. nilotica. The data were analyzed using probit analysis to determine the LC50. The result showed LC50 is 109.43 ppm for A. Squamosa seeds extract, LC50 is 171.65 ppm for T. catappa and LC50 is 203.63 ppm for A. nilotica. This result showed the biggest toxicity is seeds extract of A. Squamosa.

Pheretima javanica K. Ethanol Extract Granules’ Effects on Eosinophil Level, Immunoglobulin E Level, and Organs Histopathology in Rattus norvegicus B. Dwi Wahyuni; Joko Waluyo; Jekti Prihatin; Fersty Isna Kusumawardani; Aditya Kurniawan
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1388

BACKGROUND: Traditional medicine that might be useful to treat typhoid fever is earthworms (Pheretima javanica K.). It is important to know whether any substances contained in Pheretima javanica K. extract are toxic and have pharmacological effects, which can be observed from the eosinophil and immunoglobulin E (IgE) levels in the blood.METHODS: Thirty Rattus norvegicus B. were included in this study and were separated into 5 groups, with 6 rats in each group. T1, T2, T3, and T4 were groups of rats treated with 50, 300, 2000, and 5000 mg/kg BW of Pheretima javanica K. ethanol extract granules (PJEEG), respectively, while the control group was a group of rats treated with 0.36 mg Ciprofloxacin. The eosinophil number and the IgE concentration were detected. Physical observations were also conducted to detect any allergic symptoms, toxicity symptoms, or histopathological changes.RESULTS: Administration of PJEEG significantly increased the number of eosinophil in every group, from 49.82 cells/μL to 259.26 cells/μL on average (p<0.001). PJEEG did not significantly affect IgE levels (p=0.624). The results showed that the administration did not cause any allergic and toxicity symptoms. Histopathological analysis of rats’ kidneys and liver also showed that the administration had no significant effect on histopathological changes.CONCLUSION: The administration of PJEEG does not cause allergic symptoms, toxicity symptoms, and histopathological changes in rats. The administrations also significantly increase eosinophil number in rats’ blood. However, the concentration of IgE in rats’ blood was not significantly changed after administration of PJEEG.KEYWORDS: allergies, typhoid fever, earthworms, Pheretima javanica K., eosinophil, immunoglobulin E

Upaya Promotif Petugas Kesehatan dalam Pelaksanaan Posyandu Lansia di Puskesmas Mangli Kabupaten Jember Fitrah Hadiyati Awaliyah Tuanaya; Dwi Wahyuni; Elfian Zulkarnain
Multidisciplinary Journal Vol 1 No 1 (2018): Agustus 2018
Publisher : Pascasarjana Universitas Jember

One of the health programs which is implemented for the elderly in Indonesia is Elderly Posyandu. Elderly Posyandu emphasizes health services on promotive and preventive efforts. The Health Officer is any person who devotes himself / herself to the health sector as well as has knowledge and or skills through education in the field of health which for a certain type requires authority to do health efforts. The role of Health Officers of Elderly Posyandu is very important to realize the optimum promotive and preventive efforts in the implementation of posyandu elderly. This study aims to analyze the role of health officers in carrying out promotive efforts at elderly posyandu of Mangli Public Health Centre Jember by using qualitative method with case study design.There are 2 main informants in this research, health officer and 2 additional informants of elderly from elderly posyandu in working area of Mangli Public Health are chosen by purposive technique. The data are collected through indepth interview with main informant then verified data with triangulation of source by matching result of interview with additional informant and triangulation technique by doing observation. The results showed that the role of health officers in the implementation of promotive efforts is still less optimum.Keywords: Elderly Posyandu, Health Officer, Promotive

Pengaruh Konsentrasi dan Time Efek Ekstrak Batang Serai Wangi (Cymbopogon nardus) dalam Bentuk Spray sebagai Bioinsektisida Nyamuk Aedes aegypti Khoirul Anam; Isa Ma’rufi; Dwi Wahyuni
Multidisciplinary Journal Vol 2 No 1 (2019): Juli 2019
Publisher : Pascasarjana Universitas Jember

Dengue Hemorrhagic Fever is an insect tropic disease that always threatens the Indonesian population every year and becomes an outbreak in several regencies/cities in Indonesia after the changing seasons. The Aedes aegypti mosquito vector control effort has been carried out both physically with the PSN program or chemically using synthetic insecticides that can be directed against both adult mosquitoes and larvae. Fragrant citronella extract based on previous research contains many alkaloid compounds that can inhibit cholinesterase enzymes such as synthetic organophosphate class insecticides. The purpose of this study was to analyze the effect of concentration and time effect of citronella extract (Cymbopogon nardus) in the form of a spray as a natural bioinsecticide to control the Aedes aegypti mosquito. This research belongs to a pure research design (true experiment) with factorial design. The research unit has 3 units plus 1 unit as a control using 25 mosquitoes for each research unit. Data analysis in this study used the Repeated Measure GLM and Probit analysis. The results showed that the concentration and time effect of citronella extract had a test result of 0.0001 <0.005. The conclusion of the study was the effect of concentration and time effect of citronella extract on the death of Aedes aegypti mosquitoes. The results of the probit analysis found that the value of LT50 was 27.49 minutes after the intervention and LC50 was 3,198 ppm. Keywords: Frarant of citronella , spray, Aedes aegypti

The Toxicity of Srikaya Seed Granules (Annona squamosa L.) with Different Heating Temperatures Against the Larva of Aedes aegypti L. Rima Gloria Purwanto; Dwi Wahyuni; Joko Waluyo
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.15683

Abstract: Aedes aegypti L. is a mosquito carrying dengue virus that causes dengue fever, especially in Southeast Asia which is a tropical rain forest region which is a habitat for mosquito growth. Aedes aegypti L. mosquito control is carried out by chemical means of fogging and using abate but this control actually causes Aedes aegypti L. mosquitoes to become resistant or immune to chemical drugs so that a new breakthrough is created by making bioinsecticides biologically to eradicate the Aedes aegypti L. mosquito. with characteristics that are environmentally friendly and do not kill non-target animals and cause the Aedes aegypti L. mosquito not to become resistant. To be able to realize this desire so that the sugar cane granules extract of Annona squamosa L. containing active compounds in the form of annonain and squamosin so that they are toxic to Aedes aegypti L. mosquito larvae. Further research is to test the heating temperature level of the granules of Annona squamosa L. extract temperature of 40 ° C and 60 ° C which is more deadly of Aedes aegypti L. mosquito larvae. This research method uses a completely randomized design with four repetitions. The temperature of 60 ° C uses concentrations of 1 ppm, 6 ppm, 12 ppm, 18 ppm, 24 ppm and 30 ppm. As for the temperature of 40 ° C using concentrations of 1 ppm, 7 ppm, 14 ppm, 21 ppm, 28 ppm, and 35 ppm, each temperature compared with aquadest and abate. Data analyzed using probit analysis to determine the LC50, then followed by a statistical test paired sample T-test with SPSS to find out a significant difference between heating temperatures of 40 ° C to 60 ° C. These results then show that a higher temperature of 60 ° C has a higher level of toxicity compared to a lower temperature of 40 ° C.

EFFECT OF FEED COMPOSITION ON SPONSTIC PLASTIC WASTE DEGRADATION, SURVIVALITY AND LARVA GROWTH Tenebrio molitor L. Wachju Subchan; Lintang Mutiara Putri; Dwi Wahyuni; Sugeng Winarso
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31789

Sponge plastic is one of the plastic wastes that is difficult to degrade. Recently research reported that Tenebrio molitor L larvae have the potential capability to degrade plastic waste. But still a lack information according to the effect of feed composition on survival, and growth of T. molitor L larvae. The purpose of this study was to determine the effect of plastic feed composition on the rate of degradation of sponge plastic waste, survival, and growth of T. molitor L larvae. The method used in this study was an experimental laboratory using Completely Randomized Design (CRD) and using controls and six treatments with 3 repetition times. The parameters measured in this study were survival (%), body length growth (cm/week) and body biomass (gram/week), and degradation rate (gram/week). The data obtained from the research results will be analyzed using ANOVA which is then tested by Duncan. The results showed that feed composition had a significant effect (p=0.001) on the rate of degradation of plastic sponge waste, survival (p=0.000), and growth (p=0.001) of T. molitor L larvae. degradation, survival, and growth of T. molitor L. larvae.

Co-Authors Aditya Kurniawan Ady Soesetijo Carri Noer Fida Yanik Dewi Rokhmah Dhina Ayu Susanti Elfian Zulkarnain Erika Arifiana Erma Sulistyaningsih Febrilia Nur ‘Aini Fersty Isna Kusumawardani Firdha Novitasari Fitrah Hadiyati Awaliyah Tuanaya Imam Mudakir Intania Loren Isa Ma’rufi Isna Nur Aisiyah Jekti Prihatin Joko Waluyo Khoirul Anam Lintang Mutiara Putri Muhammad Efendi Muhammad Ramadhan Qurrotun Ayunin Reni Ikayanti Rima Gloria Purwanto Risma Galuh Suhesti Sandy Pradipta Sri Sukamto Sugeng Winarso Suratno Suratno Wachju Subchan

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