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All Journal Jurnal Sain Veteriner Jurnal Ilmu Ternak Veteriner BERITA BIOLOGI
Didik Tulus Subekti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Detection of Brugia malayi microfilaria/Larvae in mosquito using Polimerase Chain Reaction. Dyah Haryuningtyas; Didik Tulus Subekti
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.014 KB) | DOI: 10.14334/jitv.v13i3.587

Abstract

Lymphathic filariasis that is also known as elepanthiasis is caused by infestation of 3 species nematode Wuchereria bancrofti, Brugia malayi and Brugia timori. In Indonesia 70% filariasis case caused by Brugia malayi. Mosquito species from genus Anopheles, Aedes, Culex, Mansonia and Armigeres are known as vector of this disease. Microfilaria detection on mosquito is one methode to know infection rate in vector population in endemic area.The objectives of the research were to study the ability of Hha1 repeat applicable to detect microfilaria/larvae in a pool of mosquitoes and to get description of adult mosquito night biting population lived in endemic area of filariasis brugian. Mosquito as positive control used in this research come from laboratory of parasitology of FKUI. Mosquito sample from the field was from Binawara and Kolam Kiri villages, South Kalimantan province. Mosquito were trapped then identified by its species. DNA of mosquitoes was extracted and then run by the PCR using Hha 1 repeat primer. Result of the research indicated that adult mosquitoes night biting from Binawara village consist of Culex, Mansonia, Anopheles genus and from Kolam Kiri village only from Mansonia genus. Hha 1 repeat primer is applicable to detect 1 mosquito infected with microfilaria/larvae in a pool of negative mosquitoes. Mosquito samplesfrom the two villages showing negative PCR.   Key Words: Filariasis, Brugia Malayi, Vector, Microfilaria, Filaria Larve, PCR
PERBANDINGAN ANTARA ALANTOIN (5 UREIDOHYDANTOIN) DENGAN BETADINE® (POVIDONE IODINE) UNTUK PENGOBATAN LUKA BVSISI Didik Tulus Subekti
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1265

Abstract

Study on the comparison between allantoin (5 ¢ ureidohydantoin) and Betadine ® (povidone iodine) was conducted to compare and evaluate their efficacy, especially to accelerate wound (incision) healing. Treatment divided into three groups, first group is Control (without therapy), second group is allantoin treatment and the last one is Betadine ® treatment. Allantoin obtained from cattle's urine by Meissner method. The solution made of 2,4 grams of allantoin in 600 milliliters aqueous solution. Treatments (therapies) were given three times a day topically. Results showed no significant difference between allantoin and Betadine ® treatments (p > 0,05), control and the other treatments i.e allantoin and Betadine ® therapies have significantly difference (p < 0,01).
Optimization of Sybr Green Quantitative Real Time Polymerase Chain Reaction (qPCR) using Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma gondii Fitrine Ekawasti; Agus Winarsongko; Farlin Nepho; Eko Setyo Purwanto; Didik Tulus Subekti; harimurti nuradji; NLP Indi Dharmayanti; Riza Zainuddin Ahmad; Siti Sa’diah; Umi Cahyaningsih; Raden Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90867

Abstract

AbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosis
Co-Authors Agus Winarsongko Dyah Haryuningtyas Eko Setyo Purwanto Farlin Nepho Fitrine Ekawasti harimurti nuradji NLP Indi Dharmayanti Raden Wisnu Nurcahyo Riza Zainuddin Ahmad Siti Sa’diah Umi Cahyaningsih