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Didik Tulus Subekti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Detection of Brugia malayi microfilaria/Larvae in mosquito using Polimerase Chain Reaction. Dyah Haryuningtyas; Didik Tulus Subekti
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.014 KB) | DOI: 10.14334/jitv.v13i3.587

Abstract

Lymphathic filariasis that is also known as elepanthiasis is caused by infestation of 3 species nematode Wuchereria bancrofti, Brugia malayi and Brugia timori. In Indonesia 70% filariasis case caused by Brugia malayi. Mosquito species from genus Anopheles, Aedes, Culex, Mansonia and Armigeres are known as vector of this disease. Microfilaria detection on mosquito is one methode to know infection rate in vector population in endemic area.The objectives of the research were to study the ability of Hha1 repeat applicable to detect microfilaria/larvae in a pool of mosquitoes and to get description of adult mosquito night biting population lived in endemic area of filariasis brugian. Mosquito as positive control used in this research come from laboratory of parasitology of FKUI. Mosquito sample from the field was from Binawara and Kolam Kiri villages, South Kalimantan province. Mosquito were trapped then identified by its species. DNA of mosquitoes was extracted and then run by the PCR using Hha 1 repeat primer. Result of the research indicated that adult mosquitoes night biting from Binawara village consist of Culex, Mansonia, Anopheles genus and from Kolam Kiri village only from Mansonia genus. Hha 1 repeat primer is applicable to detect 1 mosquito infected with microfilaria/larvae in a pool of negative mosquitoes. Mosquito samplesfrom the two villages showing negative PCR.   Key Words: Filariasis, Brugia Malayi, Vector, Microfilaria, Filaria Larve, PCR
PERBANDINGAN ANTARA ALANTOIN (5 UREIDOHYDANTOIN) DENGAN BETADINE® (POVIDONE IODINE) UNTUK PENGOBATAN LUKA BVSISI Didik Tulus Subekti
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1265

Abstract

Study on the comparison between allantoin (5 ¢ ureidohydantoin) and Betadine ® (povidone iodine) was conducted to compare and evaluate their efficacy, especially to accelerate wound (incision) healing. Treatment divided into three groups, first group is Control (without therapy), second group is allantoin treatment and the last one is Betadine ® treatment. Allantoin obtained from cattle's urine by Meissner method. The solution made of 2,4 grams of allantoin in 600 milliliters aqueous solution. Treatments (therapies) were given three times a day topically. Results showed no significant difference between allantoin and Betadine ® treatments (p > 0,05), control and the other treatments i.e allantoin and Betadine ® therapies have significantly difference (p < 0,01).
Co-Authors Dyah Haryuningtyas