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All Journal Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Jamu Indonesia Jurnal Kedokteran Hewan Indonesian Journal of Jamu
Siti Sa’diah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Pemberdayaan Kelompok Ibu-Ibu PKK Desa Babakan, Kecamatan Darmaga, Kabupaten Bogor dalam Upaya Swamedikasi Menggunakan Tanaman Obat Siti Sa’diah; Katrin Roosita; Rudi Heryanto
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 1 No. 1 (2015): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.1.1.62-67

Abstract

Looking by the lack of information about medicinal plants and not good enough effort by the member of PKK in optimizing swamedication with medicinal plants, IbM held three types of training about swamedication using medicinal plants and monitoring the program. The purpose of this program is to improve the skills and knowledge of every member of PKK about medicial plants, grow the plants at limited space and gain the benefits from medicinal plants for inhabitant. The first training was about improving knowledge about medicinal plants and distribute seedling of medicinal plants which can plant at house yards. The second training was about production techniques of fourinds traditional medicine: jamu beras kencur, kunyit asam, galohgor and instant type of beras kencur. The last training was about techniques for organic compost from household waste. Process of evaluating and monitoring held after all the training done, then there was interview session and held a competition of producing jamu. The evaluation shows there was some improvement in consumption of beras kencur and kunyit asam families and inhabitant. Also, there was indication of transferred information from aenoughtrained member to not-trained member in informal ways. Overall, all of the programs has done with good achievement. This programs already made a significant improvement in public information about medicinal plants.
Perbandingan Ekstrak Jahe Merah (Zingiber Officinale Roscoe. Var. Rubrum), Gingerol dan Shogaol sebagai Anti-Toksoplasma terhadap Parasit Toxoplasma Gondii Secara In-Vitro Siti Sa’diah; Effionora Anwar; Mahdi Jufri; Umi Cahyaningsih
Jurnal Jamu Indonesia Vol. 4 No. 3 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1473.786 KB) | DOI: 10.29244/jji.v4i3.160

Abstract

Metode ekstraksi dan jenis pelarut yang digunakan akan berdampak pada proses penarikan komponen aktif sehingga akan berpengaruh juga pada aktivitasnya. Jahe merah (Zingiber officinale var. Rubrum) merupakan salah satu bahan rempah yang juga berpotensi sebagai tanaman obat. Salah satu khasiat ekstrak jahe merah adalah sebagai anti toksoplasma. Pada penelitian ini telah dilakukan pembuatan ekstrak jahe merah dengan dua jenis metode yaitu maserasi dan sokhletasi masing-masing menggunakan tiga jenis pelarut (etanol 30%, etanol 70% dan etanol 96%) sehingga diperoleh enam jenis ekstrak. Kemudian masing-masing ekstrak ditentukan kadar senyawa pencirinya meliputi 6-gingerol, 8-gingerol, 10-gingerol dan 6-shogaol dengan Kromatografi Cair Kinerja Tinggi (KCKT). Selanjutnya ekstrak diuji aktivitas antitoksoplasma secara in vitro terhadap parasit Toksoplasma gondii pada fase takhizoit yang ditumbuhkan pada sel vero. Penentuan aktivitas antitoksoplasma juga dilakukan pada senyawa murni 6, 8, 10-gingerol dan 6-shogaol serta kontrol positif antibiotik spiramisin. Hasilnya menunjukkan bahwa ekstrak etanol 96% dengan metode maserasi adalah yang paling baik aktivitasnya dan 6-gingerol adalah senyawa penciri yang paling berperan sebagai anti-toksoplasma.
EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Fitrine Ekawasti; Zul Azmi; Didik Tulus Subekti; muhammad ibrahim desem; Arifin Budiman Nugraha; Siti Sa’diah; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that  stored on liquid nitrogen was isolated using DNAzol™ reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.
Utilizing Program of Vacant Land on Public Facilities for Diabetes Mellitus Self-Medication Parks in Benteng Village, Bogor Regency Purnaningsih, Ninuk; Siti Sa’diah; Qodarian Pramukanto
Jurnal Jamu Indonesia Vol. 7 No. 3 (2022): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v7i3.278

Abstract

The prevalence of diabetes in Indonesia has increased substantially in the last 30 years, where individual chances of developing diabetes are higher for women than for men; and level of education is negatively related to diabetes mellitus. Independent therapy garden, is one of the functions of public park which is designed thematically for therapy (medication).The objectives of Program are: (1) Development of a participatory Diabetes Mellitus Self-therapy Garden at partner locations; (2) Cultivation training and education in using/processing of medicinal plants for self-therapy for Diabetes. Program partners are the Kecapi Posyandu RW 6 Benteng Village Bogor Regency, West Java Province. Partner problems are: (1) There are a large number of elderly people who have the potential to suffer from Type 2 Diabetes; (2) Lack of knowledge of partners and residents around partner locations about Diabetes: its characteristics, treatment and prevention; (3) Lack of skills in cultivating and processing of medicinal plants to maintain health, especially diabetes. The participatory approach was carried out by involving partners from the beginning of the program, started from discussing the activity plan, determining the location of the public facility land to be built into a therapy park, and determining program participants. The determination of the location of the park was chosen based on area that was easily visited by residents, so that they can interact with fellow residents in the park. Education about diabetes therapy was carried out by conveying information about diabetes mellitus and how to handle it. Education on the use of medicinal plants, processing them, concocting them, was carried out in practice. There has been an increase in knowledge, skills, and positive attitudes in the prevention and treatment of diabetes independently by partners. The sustainability of the program is carried out by providing diabetes self-therapy manuals, and mentoring through groups through periodic discussions.
Co-Authors Arifin Budiman Nugraha Didik Tulus Subekti Effionora Anwar Fitrine Ekawasti Ita Djuwita Katrin Roosita Latifah Kosim Darusman Mahdi Jufri muhammad ibrahim desem Ninuk Purnaningsih Qodarian Pramukanto Rahminiwati, Min Rudi Heryanto Umi Cahyaningsih Zul Azmi