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All Journal Jurnal Sain Veteriner Jurnal Medika Veterinaria
Agus Winarsongko
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Detection of Very Virulent Infectious Bursal Disease (IBD) in Chicken in West Java Ajeng Fabeana Putri; Agus Winarsongko; Heri Hoerudin; Gita sekarmila; Ahpas Ahpas; Jejen Jaelani; Wawan Gunawan; Rina Dewiyanti; Yuda Pratama; Harimurti Nuradji; Nuha Fairusya; Fitrine Ekawasti; Rahmat Setya Adji; NLP Indi Dharmayanti; Risa Indriani; Bambang Ngaji Utomo; Sri Suryatmiati
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.35819

Abstract

Infectious Bursal Disease (IBD), also known as Gumboro disease, is an acute, highly contagious disease that infects chickens and causes a high mortality rate of up to 100% in young animals. The disease is caused by Infectious Bursal Disease Virus (IBDV) of the genus Avibirnavirus, family Birnaviridae. The disease has been reported in Indonesia since 1976, and management strategies for the disease, such as vaccination, have been applied to prevent and control outbreaks in poultry farms. In this study, we conducted the detection of the disease in chickens from a farm in West Java with a mortality rate of 80%. Chickens showing clinical signs, such as sudden death, anorexia, watery diarrhea, and ruffled feathers, were necropsied, and organ samples, including the bursa Fabricius, brain, and spleen, were collected. The samples were then tested using Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) to confirm the diagnosis of IBD. Positive results were obtained in this study, highlighting the need for improved biosecurity in poultry farms in Indonesia. These results also provided a basis for further research on viral characterization to develop detection kits or vaccines for IBD using local isolates from the field in Indonesia.
Optimization of Sybr Green Quantitative Real Time Polymerase Chain Reaction (qPCR) using Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma gondii Fitrine Ekawasti; Agus Winarsongko; Farlin Nepho; Eko Setyo Purwanto; Didik Tulus Subekti; harimurti nuradji; NLP Indi Dharmayanti; Riza Zainuddin Ahmad; Siti Sa’diah; Umi Cahyaningsih; Raden Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90867

Abstract

AbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosis
Co-Authors Ahpas Ahpas Ajeng Fabeana Putri Bambang Ngaji Utomo Didik Tulus Subekti Eko Setyo Purwanto Farlin Nepho Fitrine Ekawasti Fitrine Ekawasti Gita sekarmila Harimurti Nuradji harimurti nuradji Heri Hoerudin Jejen Jaelani NLP Indi Dharmayanti NLP Indi Dharmayanti Nuha Fairusya Raden Wisnu Nurcahyo Rahmat Setya Adji Rina Dewiyanti Risa Indriani Riza Zainuddin Ahmad Siti Sa’diah Sri Suryatmiati Umi Cahyaningsih Wawan Gunawan Yuda Pratama