Claim Missing Document
Check
Articles

Found 13 Documents
Search

Isolasi dan Identifikasi Bakteri Heterotrofik di Perairan Pantai Paris Tigaras Kabupaten Simalungun Sri Winda Lestari; Ulfayani Mayasari; Rasyidah Rasyidah
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.12894

Abstract

Heterotrophic bacteria are bacteria that have a role as decomposers of waste in the form of carbon compounds such as leaves, wood from plants and animal carcasses which will produce minerals as nutrients, so that waste does not accumulate on the Paris coast. The purpose of this research was to determine the types of bacterial isolates and characteristic of heterotrophic from water Paris beach. Bacterial isolation was carried out using the spread method on nutrient agar medium. The result of isolation was 10 bacteria isolates which were then purified and observation of macroscopic morphology. The result showed that bacterial isolates SP2I1, SP4I4, SP1I7 were heterotrophic. Bacterial isolates SP2I1, SP4I4 was gram–positive with rod-shapes cells, while bacteria isolates SP1I7 was gram-negative (rod-shaped cells). Based the result of biochemical tests, morpjology observations, the bacterial isolates SP2I1, SP4I4 belongs to the genus Bacillus and isolates SP1I7 belongs to the genus Enterobacter.
Kajian Efektifitas Penggunaan Abu Sekam Padi sebagai Bahan Stabilisasi pada Tanah Lempung terhadap Nilai CBR Vella Anggreana; Rasyidah Rasyidah
Jurnal Penelitian Rumpun Ilmu Teknik Vol. 3 No. 1 (2024): Februari : Jurnal Penelitian Rumpun Ilmu Teknik
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/juprit.v3i1.3523

Abstract

One type of soil that has many problems in a construction development is clay soil. Clay soil originating from Jl. Abdul Rahman Hamid, Tenayan Raya District, Pekanbaru has a problem where there are cracks and waves on the road due to non-uniform subsidence. To deal with problems in the soil one way that can be done is by stabilization. The purpose of this study was to determine to determine the effect of a mixture of rice husk ash on the CBR value and to find the optimal mixture. The research uses the laboratory test method, by testing CBR on variations of the rice husk ash mixture amounting to 4%, 7%, and 10% of the dry weight of the soil with unfermented conditions, 7 days of incubation and 4 days of soaking. The effect of using rice husk ash on the value of CBR in unfermented conditions increased in the 4% and 7% rice husk ash mixture, but decreased in the 10% rice husk ash mixture. The influence of the use of rice husk ash mixture on 7-day fermentation increases the CBR values. Whereas in immersion conditions, the CBR value tends to decrease with increasing rice husk ash levels. The optimal mixture in CBR occurred in a mixture of 10% rice husk ash with a 7-day fermentation condition of 12.85%. Thus, it is concluded that the use of the percentage of the mixture as well as the length of the soaking and soaking days used can affect the CBR value of the soil.
Isolasi Dan Identifikasi Bakteri Proteolitik Dari Tanah Mangrove Di Pantai Pandaratan Kecamatan Sarudik Kabupaten Tapanuli Tengah Linda Eriri; Rasyidah Rasyidah; Ulfayani Mayasari
Bhinneka: Jurnal Bintang Pendidikan dan Bahasa Vol. 2 No. 3 (2024): Juli : Bhinneka
Publisher : Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59024/bhinneka.v2i3.856

Abstract

Proteolytic bacteria are bacteria that can degrade proteins by producing extracellular protease enzymes. Proteolytic bacteria can be found in mangrove soil. This is because the dry weight of mangrove leaf litter contains 61% protein. The purpose of this study was to determine the presence of proteolytic bacterial isolates from mangrove soil in Pandaratan Beach, Sarudik District, Central Tapanuli Regency and to characterize them. This study used a laboratory experimental method which was analyzed descriptively. Soil sampling was carried out at two location points I (sandy) and location II (muddy) which were isolated using SMA media with the streak plate method. Characterization includes macroscopic and microscopic observations as well as biochemical tests. The results of the study found that there were 15 isolates of proteolytic bacteria isolated from the mangrove soil of Pandaratan Beach, Central Tapanuli. The highest activity test of the proteolytic index (IP) was obtained 0.82 mm by the Micrococcus (L1) and 0.09 mm lowest by Brevibacillus (L2). A total of 11 genera of proteolytic bacteria were found, including Curtobacterium (P1 and L7), Bacillus (P2, P4, and P7), Enterococcus (P3), Brevibacillus (P5 and L2), Carnobacterium (P6), Paenibacillus (P8), Micrococcus (L1), Vagococcus (L3), Marinococcus (L4), Halobacillus (L5), Sporosarcina (L6).