Articles
PENETAPAN KADAR HIDROGEN PEROKSIDA (H2O2) PADA TAHU DENGAN METODE PERMANGANOMETRI
Muawanah Muawanah;
Nurul Afiah;
Edy Mashudi
Jurnal Medika Vol 5 No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/jmed.v5i2.179
Hidrogen peroksida (H2O2) merupakan bahan kimia yang bersifat oksidator kuat yang biasa digunakan sebagai bahan pemutih dan desinfektan. Bahan kimia ini merupakan salah satu bahan tambahan pangan yang dilarang secara resmi menurut Peraturan Menteri Kesehatan Republik Indonesia No. 33 tahun 2012 tentang Bahan Tambahan Pangan. Tujuan penelitian ini yaitu untuk menentukan kadar Hidrogen peroksida (H2O2) pada tahu. Jenis penelitian yang digunakan adalah Observasi Laboratorik dengan teknik pengambilan sampel secara Purposive Sampling. Pemeriksaan sampel tahu dilakukan di Laboratorium Kimia Kesehatan, Balai Besar Laboratorium Kesehatan Makassar dengan menggunakan metode titrasi permanganometri. Hasil penelitian dari 5 (lima) sampel tahu yang diteliti diperoleh 3 (tiga) sampel yang positif mengandung Hidrogen peroksida (H2O2) dengan masing-masing kadarnya yaitu sampel kode A sebesar 22,1514 %, sampel kode B sebesar 48,0849 %, dan sampel kode C sebesar 50,6366 %. Hal ini dapat disimpulkan bahwa 3 (tiga) sampel tahu positif mengandung Hidrogen Peroksida (H2O2) sehingga sampel tahu tersebut tidak aman untuk dikonsumsi.
ANALISIS KADAR ALKOHOL PADA BALLO ASE (Oriza sativa)
Nur Qadri Rasyid;
Hasnah Hasnah;
Nurannisa Nurannisa;
Muawanah Muawanah
Jurnal Medika Vol 7 No 1 (2022): Juni 2022
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/jmed.v7i1.287
Ballo is a traditional drink of palm wine originating from Bugis Makassar made through the fermentation process of sap, rice or fruit containing sugar. The results of fermentation produce alcohol consumed in the long term will cause cirrhosis of the liver. This study aims to determine the alcohol content contained in palm wine (ballo). This type of research was carried out by laboratory observation using the UV-Vis Spectrophotometer method. The object of this research is palm wine using 5 samples. The results of this study indicate that 5 samples contain alcohol content with sample A content of 18.67%, sample B of 28.42%, sample C of 30.67%, sample D of 8.17%, and sample E of 15.67 %. According to the regulation of the Minister of Health of the Republic of Indonesia NO. 86/MenKes/Per/VI/1997 concerning alcoholic beverages, it is explained that Group A drinks contain 1-5% ethanol content, Group B contains 5-20% ethanol content, and Group C contains 20-55% ethanol content. Based on the research that has been done, samples (A), (D) and (E) belong to group B drinks with moderate alcohol content, and samples (B) and (C) belong to group C with high alcohol content.
UJI EFEKTIVITAS PERASAN TEH (Camellia sinensis L.) CELUP TERHADAP Staphylococcusaureus
Waode Rustiah;
Andi Fatmawati;
Dewi Arisanti;
Muawanah Muawanah;
Nuraedah Hasima
Jurnal Medika: Media Ilmiah Analis Kesehatan Vol 7 No 2 (2022): Desember 2022
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/jmed.v7i2.293
Camellia sinensis L. atau yang lebih dikenal daun teh hijau sudah lama digunakan sebagai obat herbal dan juga bisa dijadikan olahan produk makanan maupun minuman. Kandungan polifenol, katekin, dan tanin yang memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui perasan teh celup (Camellia sinensis L.) dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini dilaksanakan secara eksperimen laboratorik dengan variasi konsentrasi, yaitu 25%, 50%, dan 75% serta larutan pembanding kontrol positif (antibiotic tetracycline) dan larutan pembanding kontrol negatif (aquabides), dengan masa inkubasi selama 24 jam pada suhu 370C. Sampel dalam penilitian ini adalah perasan teh hijau celup (Camellia sinensis L.) menggunakan metode difusi agar berlapis. Berdasarkan hasil penelitian pada konsentrasi 25%, 50% dan 75% tidak memiliki zona hambat. Pada kontrol negatif memiliki zona hambat 0 mm sedangkan kontrol positif memiliki zona hambat 20,2 mm. Hasil penelitian ini menunjukkan bahwa perasan teh celup tidak efektif menghambat pertumbuhan bakteri Staphylococcus aureus
ANALISIS KUALITATIF PEMANIS BUATAN SAKARIN DAN SIKLAMAT PADA AIR TAHU
Nur Qadri Rasyid;
Muawanah Muawanah;
Hasnah Hasnah;
Septiana Septiana
Jurnal Medika: Media Ilmiah Analis Kesehatan Vol 7 No 2 (2022): Desember 2022
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/jmed.v7i2.344
Sakarin dan siklamat merupakan pemanis yang sering digunakan oleh industri rumahan, karena harganya relatif murah dan dapat memberikan rasa manis yang lebih tinggi dibandingkan pemanis alami. Air tahu merupakan salah satu industri rumahan yang terbuat dari hasil ekstraksi kedelai yang memiliki rasa tidak manis sehingga pemanis buatan ditambahkan untuk meningkatkan cita rasa. Namun, pemakaian pemanis secara berlebih dapat menyebabkan efek samping yakni mengganggu sistem pencernaan, sakit kepala, migrain, tremor, diare, kehilangan daya ingat, kanker kandung kemih, pengecilan testicular dan kerusakan kromosom. Penelitian ini bertujuan untuk mengidentifikasi sakarin dan siklamat pada air tahu. Jenis penelitian observasi laboratorik sebanyak 5 sampel menggunakan metode uji warna untuk sakarin dan metode pengendapan untuk siklamat. Dari hasil penelitian yang dilakukan terhadap 5 sampel diperoleh sampel positif mengandung siklamat ditandai adanya endapan putih seperti pada kontrol positif dan negatif mengandung sakarin yang ditandai dengan tidak terjadinya perubahan warna hijau flouresens. Hasil penelitian yang telah dilakukan menunjukkan bahwa dari 5 sampel air tahu yang digunakan diperoleh 5 sampel yang mengandung siklamat dan 5 sampel tidak mengandung sakarin.
Identifikasi Dan Hitung Jumlah Bakteri Pada Telapak Tangan Penjual Gorengan
Anita Anita;
Muawanah Muawanah;
Andi Fatmawati;
Rahmawati Rahmawati;
Faisal Faisal
Lontara Journal of Health Science and Technology Vol 4 No 1 (2023): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/lontarariset.v4i1.368
The palm is the part of the body that is most often used daily to carry out various activities, which makes hands very easily contaminated by bacteria. This causes the palm of the hand can act as a medium for transmission of various types of diseases. This study aims to identify and count the number of bacteria on the palms of fried food sellers. This research technique was carried out by laboratory observation with the object of research being the palms of 10 fried food sellers in Rappocini District, Makassar City. Based on the results of research that has been carried out from 10 samples, it has been identified microscopically the form of gram-negative bacilli is red and clustered and macroscopic identification shows the shape of bacterial colonies is round, jagged with small to large sizes, while the results of calculating the number of bacteria for all samples obtained the number of bacteria in large numbers (TBUD). It can be concluded that the palms of fried food sellers in Rappocini District, Makassar City are positive for gram-negative bacilli with an amount that cannot be counted (TBUD).
PEMERIKSAAN KADAR BAHAN KIMIA OBAT (BKO) NATRIUM DIKLOFENAK PADA JAMU PEGAL LINU
Waode Rustiah;
Hasnah Hasnah;
Andi Fatmawati;
Muawanah Muawanah;
Yustikasari Masulili
Jurnal Medika Vol 8 No 1 (2023): Juni 2023
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/jmed.v8i1.370
Natrium diklofenak adalah derivat asam fenilasetat yang secara spesifik dikembangkan sebagai agen antiinflamasi. Natrium diklofenak merupakan anggota grup arilalkanoat, obat golongan non-steroidal anti-inflamatory drugs (NSAID) yang digunakan oleh masyarakat sebagai salah satu terapi artritis rematoid, osteoartritis, ankilosa, spondilitis dan inflamasi oftalmik. Natrium diklofenak biasanya banyak ditemukan dalam produk jamu kemasan. Jamu merupakan obat tradisional yang berperan penting dalam pengobatan penduduk di negara berkembang. Dalam jamu, obat ini bekerja dengan cara menghentikan produksi zat penyebab rasa sakit. Penelitian ini bertujuan untuk mengetahui ada atau tidaknya bahan kimia obat Natrium diklofenak dalam jamu pegal linu yang diperjualbelikan di pasar tradisional kota Makassar. Teknik pengambilan sampel menggunakan cara random sampling. Sebanyak 10 sampel dilakukan secara kualitatif dengan metode kromatografi lapis tipis (KLT) dan kuantitatif menggunakan spektrofotometeri UV-Vis. Hasil penelitian menunjukkan bahwa dari 10 sampel yang diteliti semuanya teridentifikasi positif natrium diklofenak yang ditunjukkan dengan nilai Rf sampel jamu dengan standar natrium diklofenak yang sama yaitu 0,85, karena selisih Rf sampel terhadap standar ≤ 0,2. Selanjutnya, dilakukan uji kuantitatif dengan spektrofotometer UV-Vis, diperolah kadar rata-rata 13,956 ppm. Dari hasil penelitian ini disimpulkan bahwa produk jamu tersebut tidak layak untuk mendapatkan izin edar.
Analisis Kadar Siklamat Pada Selai Tidak Bermerek Yang Dijual Di Pasar Tradisional Kota Makassar
Muawanah Muawanah;
Nurhidayat Nurhidayat;
Nur Qadri Rasyid;
Susi Susanti
Lontara Journal of Health Science and Technology Vol 1 No 2 (2020): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/lontarariset.v1i2.72
The jam is a semi-solid food made from fruit or other processed product. The manufacturing process can add food additives (BTP), one of which is artificial sweeteners such as cyclamate. Cyclamate can be harmful to health if consumed in levels that exceed the maximum limit.The purpose of this research was to identificate and determine the cyclamate content of unbranded jam in the traditional market town of makassar. The type of research used is laboratory observation with simple random sampling technique. This research was conducted qualitatively (depositional method) and quantitatively (gravimetric method). Based on the results of the research from 7 (seven) samples of unmerck jam there were 2 (two) positive samples containing cyclamate with cyclamate levels obtained, namely the K sample 0f 42.273,78 mg/kg and sample T3 of 49, 822. 67 mg/kg. In this study it can be concluded that 2 (two) samples of unbranded jam contained cyclamate levels that exceed the maximum limit set by BPOM No. 4 of 2014, namely 1000 mg/kg and so it is not safe for consumption.
Analisis Kualitatif Residu Pestisida Pada Bahan Pangan Dengan Menggunakan Metode Kromatografi Lapis Tipis (KLT)
Muawanah Muawanah;
Nur Qadri Rasyid
Lontara Journal of Health Science and Technology Vol 2 No 2 (2021): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/lontarariset.v2i2.222
Vegetables and fruits are foodstuffs whose cultivation process requires pesticides. In addition to farmers who apply pesticides, pesticide poisoning can also be experienced by people who consume agricultural products including vegetables through the pesticide residues contained in them, so that it will have a negative impact on human health who consume them continuously. The purpose of the study was to determine the presence of pesticide residues in foodstuffs. This research is a laboratory observation with a simple random sampling technique. In this study, a qualitative analysis of organophosphate and carbamate pesticide residues in food ingredients was carried out using the Thin Layer Chromatography (TLC) method. Based on the results of the study, from 15 (fifteen) samples of vegetables, no organophosphate chlorpyrifos pesticide content was detected, but the carbamate group was detected in samples of spinach, cabbage, and carrots marked with spots and the Rf value was the same as the Furadan standard. Thus, it is hoped that the community will wash the vegetables before they are processed and consumed.
Identifikasi Salmonella Sp Pada Saus Cabai Pentolan yang Diperjualbelikan Di Kecamatan Mamajang Kota Makassar
Tuty Widyanti;
Muawanah Muawanah;
Indriany Iyonu
Lontara Journal of Health Science and Technology Vol 2 No 2 (2021): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/lontarariset.v2i2.225
Sauce is a flavoring ingredient that is usually added to food. In the chili sauce processing process, it is possible that the raw materials used are not washed clean and the place used to store chili sauce is in the form of bottles or containers that do not undergo a sterilization process after use. One of the bacteria that contaminates chili sauce is Salmonella sp which can cause digestive tract disorders. The purpose of the study was to identify Salmonella sp in the pentolan chili sauce that was traded in the Mamajang sub-district, Makassar city, which was a laboratory observation using research methods, namely culture and biochemical tests. The research sample used was pentolan chili sauce that was traded in the Mamajang sub-district of Makassar city as many as 10 samples with the sampling technique of random sampling. Based on the research that has been done from 10 samples of pentolan chili sauce, positive results were obtained for Salmonella sp. It can be concluded that the pentolan chili sauce sold in the Mamajang district of Makassar city has been contaminated by Salmonella sp.
Identifikasi Dan Hitung Jumlah Bakteri Pada Telapak Tangan Penjual Gorengan
Anita Anita;
Muawanah Muawanah;
Andi Fatmawati;
Rahmawati Rahmawati;
Faisal Faisal
Lontara Journal of Health Science and Technology Vol 4 No 1 (2023): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar
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DOI: 10.53861/lontarariset.v4i1.368
The palm is the part of the body that is most often used daily to carry out various activities, which makes hands very easily contaminated by bacteria. This causes the palm of the hand can act as a medium for transmission of various types of diseases. This study aims to identify and count the number of bacteria on the palms of fried food sellers. This research technique was carried out by laboratory observation with the object of research being the palms of 10 fried food sellers in Rappocini District, Makassar City. Based on the results of research that has been carried out from 10 samples, it has been identified microscopically the form of gram-negative bacilli is red and clustered and macroscopic identification shows the shape of bacterial colonies is round, jagged with small to large sizes, while the results of calculating the number of bacteria for all samples obtained the number of bacteria in large numbers (TBUD). It can be concluded that the palms of fried food sellers in Rappocini District, Makassar City are positive for gram-negative bacilli with an amount that cannot be counted (TBUD).