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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia VALENSI Current Biochemistry Indonesian Journal of Pharmaceutical Science and Technology Justek : Jurnal Sains Dan Teknologi Jurnal Farmamedika (Pharmamedica Journal) Indonesian Journal of Applied Research (IJAR)
Djarot Sasongko Hami Seno
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University, Bogor 16680,West Java Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Immunology & microbiology Mathematics Medicine & Pharmacology

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Journal : Current Biochemistry

 1 
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Dwitasari, Oczhinvia; Hami Seno, Djarot Sasongko; Safithri, Mega
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Phytochemical Analysis, α-glucosidase Inhibition Activity in-vitro and Enzyme Kinetics of Ethyl Acetate and Hexane Extracts of Graptophylum pictum (L.) Griff Waras Nurcholis; I Made Artika; Djarot Sasongko Hami Seno; Dimas Andrianto; Apipah Aprianti; Fina Febrianti; Inawati Inawati; Antonius Padua Ratu; Arya Arendra
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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The species Graptophylum pictum (L.) Griff, also known as “daun ungu” in Indonesia, is a traditional herbaceous plant believed to have antidiabetic potential. The number of people in the world with diabetes has increased dramatically over the recent years. The treatment of type II diabetes is complicated by several factors inherent to the disease. Elevated postprandial hyperglycemia is one of the risk factors and the intestinal digestive enzyme α-glucosidase plays a vital role in carbohydrate metabolism. One of the antidiabetic therapeutic approaches which reduces the postprandial glucose level in blood is by the inhibition of α-glucosidase. In this study, phytochemical analysis, α- glucosidase inhibitory activity and enzyme kinetics of ethyl acetate- and hexane extracts of G. pictum were evaluated with the aim to analyze its antidiabetic potential. Phytochemical analysis revealed the presence of tannins, steroids, and alkaloids. Steroids were present in ethyl acetate extract but absent in hexane extract, while alkaloids were present in hexane extract but absent in ethyl acetate extract. The ethyl acetate and hexane extracts had 30.68 and 49.82 % inhibitory effect on α-glucosidase activity respectively. The kinetics of glucosidase enzyme of ethyl acetate and hexane extracts were determined by Lineweaver Burk plots. These exhibited uncompetitive and noncompetitive inhibition to alpha-glucosidase activity respectively. From the enzyme assay, we infer that ethyl acetate and hexane extracts of G. pictum contain potential α-glucosidase inhibitors that have the potential to be exploited for use in the treatment of diabetes
Identification of Aroma Gene (Mutated badh2) and Properties of Aroma on Aromatic BC5F2 Ciherang Jap Mai Cing; Djarot Sasongko Hami Seno; Tri Joko Santoso
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Aromatic rice varieties have some weaknesses such as low productivity, and less resistant to pests and diseases. This study aimed to obtain homozygous strain of BC5F2 Ciherang aromatic through the identification of aroma gene (mutated badh2) and properties of the aroma. Ciherang paddy (nonaromatic paddy) was used as the female parent, whereas Mentik Wangi paddy (aromatic paddy) was used as the male parent. The experiment was conducted in BC5F2 because it is expected to generate plants with properties 98.4% close to female parent. The DNA from five strains of paddy plants BC5F2Ciherang X Mentik Wangi was isolated by a modified CTAB method. The concentration of DNA was determined by measuring absorbance at 260 nm wavelength, while its purity was determined from the ratio of the absorbance at a wavelength of 260/280 nm. PCR-based molecular selection was done by using the Bradbury primers. PCR results showed that of the 250 samples, there were 66 samples had DNA fragment of the same size as that of Mentik Wangi, i.e. 257 bp, 67 samples had the same size as the DNA fragment of Ciherang, i.e. 355 bp, and 117 samples had the same size with the both of DNA fragments, i.e. 257 bp and 355 bp. Plants with amplified 257 bp DNA fragment was subjected to leaf aroma test using 1.7% KOH. The results showed that 42 positive samples, out of 66 samples. Samples positive on leaf aroma test were tested again on rice aroma test. Rice aroma test results showed the majority (85.4%) samples that are positive on leaf aroma test is also positive on the rice aroma test. 
In Silico Analysis of Curcuma longa Against PCAF Histon Asetiltransferase Artha Vinsentricia; Djarot Sasongko Hami Seno; Maria Bintang
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Curcumin turmeric (C. longa) has been found capable of inhibiting p300/CBP associated factor (PCAF) histone asetyltransferase (HAT) as a cancer biomarker. However, the molecular mechanism of inhibition is unkown. This study is aimed to analyse the molecular inhibition mechanisms by in silico method. The analysis showed that curcumin’s Gibbs free energy (ΔG) was stable and capable of binding amino acid residues believed to be active site of PCAF HAT. Curcumin is predicted to be a PCAF HAT inhibitor, and further in vitro analysis is required.
The Activity of Wungu Leaf (Graptophyllum pictum (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice Hayatul Rahmi; I Made Artika; Norman Razief Azwar; Djarot Sasongko Hami Seno; Waras Nurcholis
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.
Perbandingan Pertumbuhan Butyrivibrio fibrisolvens E14 Varian Sticky dan Loose Djarot Sasongko Hami Seno; John Douglas Brooker
Current Biochemistry Vol. 1 No. 2 (2014)
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A number of Butyrivibrio fibrisolvens strains have been reported to attach cellulose fibres, feed and other particles in the rumen, as well as to sheep rumen epithelia. In this research, B. fibrisolvens E14 variants sticky (S) and loose (L) were used to study the mechanism of B. fibrisolvens attachment to surfaces, especially to plant fibre. Results may be useful in enhancing plant fiber degradation within the rumen, or the use of plant biomass as energy source. The two cell types were compared; studies included physical and growth characteristics in defined, solid or liquid medium containing various carbon sources, the presence of compounds that may induce or inhibit attachment, and their phenotypic stability. Compared to the non-adhering L cells, the adhering S cells were shinier, spherical, more intensely pigmented (yellow), more firmly attached to the agar surface and could only be removed with scraping. After longer incubation, the cells were released from the agar but the colonies tended to stick together, and only became separable when further incubated. In contrast, the L cells were non spherical, loosely attached to the agar and separable at all stages of growth. In liquid medium, the S cells tended to clump during the early stages of growth, and be dispersed at later stages. The L cells were dispersed throughout the medium at all stages of growth. The phenotypes of the 2 variants were stable; both variants maintained their characteristics through multiple passages on solid and in liquid medium. The presence of molecules that induced attachment of S or inhibited attachment of L cells were not detected.
Pengembangan Nontransgenik F1 dan Bc1f1 Padi Ciherang Toleran Genangan secara Site-Directed Crossing Djarot Sasongko Hami Seno; Satya Nugroho; Tri Joko Santoso; Joel Rivandi Sinaga; Euis Marlina; Dimas Adrianto; Rudi Munzirwan; Aniversari Apriana; Zainal Alim Mas'ud
Current Biochemistry Vol. 1 No. 3 (2014)
Publisher : IPB University

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The development of submergence tolerant rice varieties is urgently required to maintain the stability of future food production, to anticipate the unpredictable global climate changes. Due to in-economical agronomic traits of native submergence tolerant varieties for large scale cultivation, submergence tolerance gene (sub1) must be introduced into popular high-yielding rice variety, such as Ciherang. To develop new submergence tolerant variety with good agronomic traits as those of Ciherang, in this research, submergence tolerance gene (sub1) was introduced into Ciherang variety. To avoid strict GMO regulation, gene introduction was carried out through site-directed crossing. Donor sub1 was crossed with Ciherang host. The selected F1 progenies were further backcrossed to Ciherang 4 x to obtain BC5F1 progeny having ~98% agronomic traits of those of Ciherang. In every cross/backcross generation, submergence test was performed, followed by sub1 marker-assisted PCR. F1 and BC1F1 submergence-tolerant Ciherang were successfully constructed. Co-dominant RM464A marker was not able to discriminate between host, donor, and progenies (F1 and BC1). Co-dominant RM219 maker showed slightly different size between donor and host amplicon, but it was difficult to see their heterozygous progenies. Both C173 and AEX1 dominant markers were able to show sub1 introgression from donor to host. PCR results confirmed that progenies-submergence tolerance was due to sub1 introgression, not escape mechanisms. AEX1 was chosen for subsequent experiments. Backcross until BC5 is in progress, to obtain maximum host retention for engineering new submergence tolerant varieties with good agronomic traits as those of Ciherang.
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Oczhinvia Dwitasari; Djarot Sasongko Hami Seno; Mega Safithri
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Co-Authors . Sugihartati . Taufiq A.A. Ketut Agung Cahyawan W Abdullah, Wais Akhmad Endang Zainal Hasan Aniversari Apriana Aniversari Apriana Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Apipah Aprianti Artha Vinsentricia Arya Arendra Bambang Padmadi Bambang Pontjo Priosoeryanto Bram Kusbiantoro Cika Hilda Fransiski Dewi Praptiwi Dimas Adrianto Dimas Adrianto DIMAS ANDRIANTO Dwitasari, Oczhinvia Dwitasari, Oczhinvia Euis Marlina Fina Febrianti Hayatul Rahmi Helmy Ramadhan Al Anshary I MADE ARTIKA Inawati Inawati Inda Setyawati, Inda Jap Mai Cing Joel Rivandi Sinaga John Douglas Brooker LAKSMI AMBARSARI MARIA BINTANG Mega Safithri Muhammad Taufan Fatahajudin Mustika Weni Najmah Najmah Norman Razief Azwar Oczhinvia Dwitasari Popi Asri Kurniatin Rini Kurniasih, Rini Riyan Alifbi Putera Irsal Rizki Fitria Rudi Munzirwan Satya Nugroho Satya Nugroho Tri Joko Santoso Tri Joko Santoso Waras Nurcholis Zainal Alim Mas'ud Zainal Alim Mas'ud