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Journal : Borneo Journal of Medical Laboratory Technology

Trombositosis: Faktor Risiko Peningkatan Aterosklerosis pada Perokok Pasif di Kecamatan Pahandut Kota Palangka Raya Rinny Ardina
Borneo Journal of Medical Laboratory Technology Vol 1 No 1 (2018): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.983 KB) | DOI: 10.33084/bjmlt.v1i1.457

Abstract

Passive smokers that exposure to cigarette smoke in their house has been reached 78,4%. Inhaled of cigarette smoke in the room was more dangerous than in an open place. Every puff of cigarette smoke from active smoker contains 1015 of free radicals which can cause oxidative stress. The presence of free radicals will trigger of activation of inflammatory genes, and then they can be activated of endothelial cells, dysfunction, and injury. Oxidative stress also stimulates large production of platelet (thrombogenesis) and followed by excessive formation of fibrin and thrombin. So that can cause thrombus in endothelial tissue, and it subsequently develops into atherosclerosis. This study aimed to describe thrombocytosis as a risk factor for increased of atherosclerosis in passive smokers in Kecamatan Pahandut, Kota Palangka Raya. This study used a descriptive observational method with a cross-sectional design. The sample obtained by 45 people with purposive sampling technique with criteria such as respondents were passive smokers, housewives, exposed to cigarette smoke more than five years, no history of the disease, and willing to become respondents. Examination of platelet counts was analyzed by an automatic method using Hematology Analyzer. The results showed that 6.7% of passive smokers had high platelet counts (thrombocytosis). High platelet counts in 6.7% of passive smokers caused by exposure of cigarette smoke from active smokers can endanger passive smoking, especially the risk of atherosclerosis. Therefore, a preventive and educative method for both passive and active smokers are needed to prevent the dangers of cigarette smoke to the risk of atherosclerosis
Efek Merokok Berat terhadap Jumlah Leukosit dan Jenis Leukosit pada Pria Usia Produktif di Kelurahan Tanjung Pinang Kota Palangka Raya Rinny Ardina; Nada Soraya
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.447 KB) | DOI: 10.33084/bjmlt.v1i2.708

Abstract

Prevalence of productive men smokers in Kalimantan Tengah in 2013 has been reached 64.9% with a mean of smoking about 12.3 cigarettes a day. Cigarette smoke contains 1015 of oxidative free radicals which can cause oxidative stress. It will trigger of activation of inflammatory genes and increase of cytokines release by increasing of leukocytes and activate some of the differential leukocytes. Continual inflammation can cause atherosclerosis and coronary heart disease. This study aimed to determine the effects of heavy smoking on white blood cell count and differential leukocyte count in productive men in Kelurahan Tanjung Pinang, Palangka Raya. This study used a descriptive observational method with a cross-sectional design. The sample obtained by 28 people with purposive sampling technique with criteria: men, active smoker, smoking more than ten years, working as a farmer, fish farmer, or laborer, and willing to become a respondent. White blood cell count and differential leukocyte count were analyzed by an automatic method using Hematology Analyzer. The results showed that 3.6% of active smokers had high white blood cell count and others had low white blood cell count (3.6% ). Whereas differential leukocyte count showed that 2 persons had high of eosinophils (7.10%), 5 people had low of eosinophils (17.9%), one person had little of neutrophils (3.6%), 2 persons had high of lymphocytes (7.20%), 10 people had little of lymphocytes (35.7%), and 15 people had high of monocytes (53.6%). To reduce the risks, preventive and educative actions from medical personnel are needed for active smokers and their families.
Pengaruh Variasi Waktu Inkubasi terhadap Kadar Hemoglobin Menggunakan Metode Sahli Rinny Ardina; Yemimasike Putri
Borneo Journal of Medical Laboratory Technology Vol 2 No 1 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.66 KB) | DOI: 10.33084/bjmlt.v2i1.1084

Abstract

Several clinical laboratory and community health centers in Palangka Raya still use a simple method to determine hemoglobin levels with Sahli method. There are mamy differences in procedures related to incubation time in Sahli method of hemoglobin examination. There are those who don’t use incubation, incubation for 3 minutes or incubation for 5 minutes. This study aimed to determine the effect of icubation time on hemoglobin levels using the Sahli method. This study was experimental study, where hemoglobin levels are measured by Sahli method with incubation time variations of 0 minutes, 3 minutes, 5 minutes, 8 minutes, and 10 minutes. A total of 21 blood samples were obtained by purposive sampling technique. The data obtained were statistically analyzed using the One Way ANNOVA test. The results showed there are significant effect of incubation time (0 minutes, 3 minutes, 5 minutes, 8 minutes and 10 minutes) on hemoglobin levels using Sahli method (p = 0.00). Increased hemoglobin levels occur with increasing incubation time. Where the lowest value of the average hemoglobin level at the measurement of 0 minutes was 10.9 g / dl and the highest value on the measurement of 10 minutes was 14.2 g / dl. It is important to laboratory personnel to incubate blood according to the procedures established by the Sahli method, because errors in the analytical stage contribute 25% to the causes of errors in laboratory result.
APTT (Activated Partial Thromboplastin Time) dan (Prothrombin Time) pada Penderita Diabetes Melitus Tipe 2 di RSUD dr. Doris Sylvanus Palangkaraya Rinny Ardina; Fera Sartika; Lidya Prihatini Nainggolan
Borneo Journal of Medical Laboratory Technology Vol 2 No 2 (2020): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.941 KB) | DOI: 10.33084/bjmlt.v2i2.1384

Abstract

Patients with type 2 diabetes mellitus (T2DM) have a high risk of atherothrombotic events. Hyperglycemia and other metabolic disorders in T2DM are associated with abnormalities of hemostasis system and thrombosis that contribute to cardiovascular disease. Routine laboratory tests to examine the hemostasis system are Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT). This study aimed to describe the abnormalities of APTT and PT in patients with T2DM in RSUD dr. Doris Sylvanus Palangkaraya. Twenty subjects with T2DM in RSUD dr. Doris Sylvanus Palangkaraya were obtained using purposive sampling techniques and blood samples were examined with a COATX Biosystem coagulation using photocolorimetric method. This study found that was 70% APTT are shortened, 5% APTT are prolonged and 25% APTT were normal. While the PT results were 25% PT are shortened and 75% APTT were normal. Abnormalities of APTT and PT in T2DM patients in RSUD dr. Doris Sylvanus Palangkaraya showed an abnormality of the coagulation system so as to allow a tendency for bleeding and cardiovascular disease in T2DM patients. Routine coagulation test should be continued to help better diabetes mellitus management in order to prevent micro or macrovascular complications.
Perbedaan Hasil Pemeriksaan Hemoglobin dengan Point of Care Testing (POCT) pada Sampel Darah Vena dan Kapiler Reza Widianto; Dwi Purbayanti; Rinny Ardina
Borneo Journal of Medical Laboratory Technology Vol 4 No 1 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i1.2956

Abstract

On examination of hemoglobin with POCT generally use capillary blood. The use of capillary blood as a test sample has a deficiency, namely the possibility of dilution in capillary blood samples caused by a deep puncture so that the blood that comes out is not smooth and usually the fingers will be pressed or sequenced. This condition can cause blood dilution by tissue fluid, so the test results will tend to be low. The purpose of this study was to find out the difference in hemoglobin results with POCT in venous and capillary blood samples. This study uses descriptive analytical methods that aim to describe or describe a situation objectively, and then describe it. The results showed the average hemoglobin level in the venous blood sample was 13.2 g/dl. While in capillary blood samples 12.9 g / dl. The results of the independent t-test obtained a significance value of 0.224 (p > 0.05) so it can be concluded that there is no significant difference between the results of hemoglobin levels examination using venous blood samples and capillary blood samples.
HUBUNGAN ANTARA BEKERJA MALAM DENGAN KONTROL GLIKEMIK PADA PEDAGANG PASAR TRADISIONAL DI KOTA PALANGKA RAYA Dwi Purbayanti; Buana Hairunisa; Rinny Ardina; Noor Fadillah; Nurhalina
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 1 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bekerja pada malam hari dapat berdampak negatif terhadap kesehatan pekerja akibat gaya hidup yang tidak sehat dan terganggunya irama sirkadian tubuh yang dapat menimbulkan terganggunya metabolisme tubuh dan homeostasis glukosa. Bekerja malam berkaitan dengan peningkatan risiko diabetes melitus tipe 2. Penelitian ini bertujuan untuk mengetahui dampak dari aktivitas bekerja malam pada pedagang pasar subuh yang sehat terhadap kontrol glikemik melalui kadar HbA1c. Metode penelitian yang digunakan adalah metode deskriptif observasional, dengan jumlah pedagang pasar subuh yang telah mengisi Informed consent dan memenuhi kriteria inklusi-eksklusi adalah sebanyak 50 orang yang diperoleh menggunakan teknik purposive sampling. Kadar HbA1c diukur pada sampel darah vena dengan antikogulan EDTA menggunakan alat Quo-Lab A1c. Analisis regresi digunakan untuk mengetahui hubungan antara kadar HbA1c dengan jenis kelamin, umur, masa kerja, konsumsi kalori tinggi pada malam hari, dan aktivitas fisik. Hasil diperoleh sebagian besar pedagang memiliki kadar HbA1c normal yaitu 42 orang (84%), dan terdapat 6 orang (12%) dengan kategori prediabetes dan 2 orang (4%) dengan kategori diabetes. Terdapat hubungan positif signifikan (P-value = 0,041) antara kadar HbA1c dengan masa kerja dan konsumsi makanan berkalori tinggi pada malam hari (P-value = 0,006). Bekerja malam pada pedagang pasar subuh jalan Seram di Kota Palangka Raya memiliki dampak merugikan terhadap kontrol glikemik.