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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Omni-Akuatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Molekul: Jurnal Ilmiah Kimia Scripta Biologica Jurnal Akuakultura Universitas Teuku Umar IJP (Indonesia Jurnal Perawat). Darma Sabha Cendekia

Yulia Sistina

Fakultas Biologi Universitas Jenderal Soedirman Purwokerto

Author-ID : 2600077

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Nursing

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Articles

Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture Farida Nur Rachmawati; Ridwan Affandi; Yulia Sistina
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Eel population in nature reaches critical number, so that culture strategy is urgently needed to fulfil the high demand of this fish. A shortcut to get functional male, which proven difficult to be founded from natural catching, is masculinization. This research aimed to induce masculinization of tropical eel, Anguilla bicolor McClelland supplemented with various doses of 17α-methyltestosterone during a month culture in freshwater (0 ppt) or brackish water (10 ppt). The eel was grouped and fed with supplementation of 17α-methyltestosterone at various doses, depending upon treatments, namely 0 mg Kg-1 diet (control), 40, 80 or 120 mg Kg-1 diet. Eels size were similar, at approximately 16,78 g ± 0,62 in weight and 25,38 cm ± 0,15 in length were either culture in freshwater or brackish during the experiment for eight weeks. Sex gonad, based on anatomical histological structures, Eye Index and Fin Index were measured after time culture treatment achieved, as well as body length, weight, eye diameter and the length of the pectoral fin were measured. Results showed that supplementation 17α-methyltestosterone 80 mg/Kg diet culture in brackish water has the highest number of male (90%). This study proven that, the hormone was effective for masculinization in eels, It useful for masculinization in eels. Results proved that the 17α-methyltestosterone highly significant (P<0.01) effect on the Eye index (3.63 – 5.14) and Fin Index (3.03 – 4.08) of eels. This study concluded, that 17α-methyltestosterone more effective in improving the number of males in brackish water than in freshwater culture.

Masculinization of Tropical Eel Anguilla bicolor McClelland in Different Population Density Farida Nur Rachmawati; Yulia Sistina
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 25, No 3 (2020): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.25.3.97-102

In general, female eel dominates the results of catching eel in the river. Male fish dare rarely found in nature, therefore masculinization is necessary for obtaining in males. The administration of 17α-methyltestosterone to masculinize Anguilla bicolor McClelland. It is a synthetic anabolic-androgenic steroid which has potential to endocrine disrupter that disturbed function of normal reproduction in human or animal. It urgently needed that a masculinization technique needs to study the use of an environmental factor. Population density is one of the environmental factors that influence gender determination (ESD-environmental dependent sex determination). This will result in increased cortisol secretion, which will further stimulate the synthesis of 11-KT steroids that affect male gonad differentiation. This study aims to induce masculinization in tropical eel Anguilla bicolor McClelland in different density. Three treatments and three replicates conducted the research. The treatments were one fish.48 L-1, two fish.48 L-1 and three fish.48 L-1. Eels size was approximately similar, at 16,78 g±0,62 in weight, and 25,38 cm±0,15 in length were either culture in brackish water for eight weeks. The results showed that density population treatment significantly increased the Fin Index (P<0.05), but no sign for Eye Index, GSI, HSI, and testosterone level. The highest male population (77,8%) achieves at the highest density treatment, three fish.48 L-1 population density. It can be concluded, based on reproductive observations, population density does not affect gonad maturity/puberty, but the high population density (3 fish.48 L-1)stimulates Anguilla bicolor McClelland masculinization.

The Effect of Endosulfan in GnRH and GtH Genes Expression of Male Hard-Lipped Barb (Osteochilus vittatus C.V.) asrul sahri siregar; pudji astuti; yulia sistina; norman arie prayogo
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

The presence of pollutants in water makes the environment toxic to all living biota and affects reproduction in fish. One of such pollutants is endosulfan, which is an off-patent organochlorine insecticide, widely used in various agricultural activities. Endosulfan is extremely toxic to fish and all other aquatic organisms, showing a range of chronic effects, including genotoxicity, and reproductive issues. Therefore, the aim of the research was to find the effect of endosulfan on cGnRH, sGnRH, GtH-I and GtH-II gene expression inmale hard-lipped barb fish. These fish were kept in aquarium containing several levels of endosulfan (0 mg/L [control]; 0.88 mg/L [low level]; 1.76 mg/L [medium level]; and 2.64 mg/L [high] level) for 60 days. The effects of endosulfan on GnRH performance on the fishwere evaluated by the expressions of these genes; cGnRH-II, sGnRH, GtH-I, and GtH-II. In the 8th week, there was a decrease in the expression of all the four genes in fish with high dose of endosulfan compared with the control group (P < 0.05). These findings showedthat endosulfan inhibits the expression of cGnRH, sGnRH, GtH-I and GtH-II genes in male hard-lipped barb.

UKURAN ORGAN SISTEM REPRODUKSI ITIK JANTAN YANG DISUPLEMENTASI PROBIOTIK MEP+ BERBAGAI DOSIS SELAMA 30 HARI Chelsy Rirgiyensi; Yulia Sistina; Farida Nur Rachmawati
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Probiotics MEP+ can increase fowl weight and weft efficiency, therefore it is important to know probiotics MEP+ effect at different dosage toward reproduction aspect. This research aimed to examine duck reproduction organ size suplemented with probiotics MEP+ with different dosage within 30 days. This research used Completely Randomized Design (CRD) with 4 treatments with different dosages within 30 days which was without probiotic’s application or control (K), 0,75 ml/kg wefts (P1) dose, 1,5 ml/kg wefts (P2) dose, a n d 3 ml/kg wefts (P3) dose. Each treatment repeated 8 times. Total 40 ducks raised in floor dry cage system. At 31st day of treatment duck reproduction system organ was measured. Whole results show increase average data (±SD) for weight of both right and left testis, and liver weight with highly probiotics dosage it, however the analysis result statistic not significant (P>0,05) except weight of right left testis with duck weight or gonadosomatic indeks (GSI) were very significant (P<0,01) among all treatment at different dosages was compared control. The results is confirmed that probiotic's MEP+ treatment with different dosages within 30 days gave no effect towards duck reproduction system organ size except to gonadosomatic indeks (GSI) male duck.

Keberhasilan Ginogenesis Ikan Tawes (Barbonymus gonionotus Blkr.) pada Dua Dosis Iradiasi UV (λ 254 nm) dengan Kejut Panas 40 C Reni Apriyani; Suhestri Suryaningsih; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.346

Laporan ini memverifikasi keberhasilan protokol tiga tahap ginogenesis tawes melalui inaktivasi genom jantan dengan iradiasi ultra violet (UV), fertilisasinya, dilanjutkan diploidisasi zigot dengan kejut panas (400 C) selama 60 detik. Eksperimen Rancangan Acak Lengkap, tujuh perlakuan yaitu, kontrol positif (fertilisasi normal); kontrol negatif1 telur difertilisasi dengan milt encer yang di UV 1983,348 J/m2; kontrol negatif2 dosis iradiasinya 3966,96 J/m2; ginogenesis1 kontrol negatif1 lalu dikejut panas pada 10 menit pasca fertilisasi; ginogenesis2 ginogenesis1 beda waktu kejut pada 15 menit pasca fertilisasi; ginogenesis3 kontrol negatif2 lalu dikejut pada 10 menit pasca fertilisasi; dan ginogenesis4 ginogenesis3 beda waktu kejut pada 15 menit pasca fertilisasi. Materi gamet segar diperoleh dari induk yang diinduksi GnRH analog dan domperindon dosis 1,5 ml/kg bobot induk intramuskuler, 6-10 jam sebelumnya. Milt segar diencerkan 100 x dalam larutan Ringer. Data dianalisis dengan uji Anova, menggunakan program SPSS. Hasil penelitian menunjukkan bahwa data fertilitas, data penetasan dan data kelangsungan hidup yang terbukti homogen (p>0,05), menunjukkan bahwa ketujuh perlakuan secara statistik fertilitas, penetasan, dan kelangsungan hidupnya tidak nyata (p>0,05) antar pelakuan. Data persentase morfologi larva abnormal tawes menunjukkan bahwa perlakuan yang dicobakan memberikan pengaruh yang sangat nyata (P<0,01). Secara keseluruhan dapat disimpulan bahwa, keempat perlakuan mitoginogenesis yang diujikan efektif menghasilkan larva mitoginogenesis ikan tawes walaupun efektifitasnya masih tergolong rendah. Efektivitas perlakuan dibuktikan dari signifikansi data morfologi larva abnormal dariklompok kontrol negatif membuktikan efektivitas dosis inaktivasi genetis jantan dan morfologi larva normal hasil perlakuan ginogenesis membuktikan efektivitas diploidisasi kejut panas tidak berbeda dari morfologi kontrol positif.

FERTILITAS DAN VIABILITAS EMBRIO TELUR ITIK YANG INDUKNYA DIBERI PAKAN SUPLEMENTASI PROBIOTIK Yunita Rusidah; Yulia Sistina; Ismoyowati Ismoyowati
Indonesia Jurnal Perawat Vol 2, No 2 (2017): Indonesia Jurnal Perawat
Publisher : Universitas Muhammadiyah Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26751/ijp.v2i2.473

AbstrakTujuan penelitian ini adalah untuk mengetahui efek perbedaan dosis pada pakan dengan suplementasi probiotik dan mengetahui dosis yang paling efektif terhadap fertilitas dan viabilitas embrio telur itik (Anas platyrhynchos). Materi penelitian terdiri dari Itik petelur (Anas platyrhynchos) sebanyak 100 ekor dengan rasio sex, jantan : betina = 1 : 5. Bahan pakan sesuai standar SNI formulasi H dan probiotik MEP+. Penelitian eksperimental ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 4 ulangan. Perlakuan terdiri dari : Ro (Kontrol), R1 (Probiotik 0,075 cc/kg pakan), R2 (Probiotik 1,5 cc/kg pakan), R3 (Probiotik 3 cc/kg pakan). Hasil penelitian menunjukan bahwa persentase fertilitas telur itik, secara statistik tidak berbeda nyata (p > 0,05). Viabilitas embrionya hingga hari ke-21 inkubasi hasilnya tidak berbeda nyata (p > 0,05). Persentase penetasan dan viabilitas embrio setelah periode hari ke-21 hasilnya berbeda nyata (p < 0.05), dilihat dari rataan mortalitas embrio terendah perlakuan dosis 0,75 cc/kg pakan (R1) dan penetasan telur itik tertinggi perlakuan dosis 1,5 cc/kg pakan (R2). Parameter pendukung berat telur dan panjang telur secara sangat nyata (p < 0,01) dipengaruhi perlakuan, sedangkan analisis data parameter pendukung meliputi, indek telur, lebar telur, kualitas DOD dan bobot DOD, hasilnya tidak berbeda nyata (p > 0,05). Hasil uji regresi korelasi data membuktikan bahwa fertilitas dan penetasan, berat telur dan penetasan, bobot DOD dan berat telur terdapat korelasi lemah diantaranya (r < 0,06). Key words : Fertilitas, Viabilitas Embrio, probitiotik MEP+ AbstractThis study aims to determine the effect of probiotics as parental food supplementation on fertility and embryo viability of duck eggs (Anas platyrhynchos). More specifically the study aimed to know the dose as well as the most effective dose applied as treatment on the fertility and viability of embryonic duck (Anas platyrhynchos) eggs. Material experimental unit consist of 1 male with 5 female (1:5). Feed ingredients based to SNI standard H formulation and probiotics MEP+. Experimental design usedwas Completely Randomized Design (CRD), four treatments,each with four replications of unit experiments. Experimental unit consist of 1 male with 5 female (1:5). Treatments were : Ro (Control), R1 (0,75 ml probiotic/kg feed), R2 (1.5 ml probiotic/kg feed), R3 (3 ml probiotic/kg feed). Results showed that egg fertility was statistically no significant different (p > 0.05) among treatments. Embryo viability up to days 21 of incubation was also statistically no significant different (p > 0.05). Hatching rate among treatments as well as embryo viability data after days 21, however, statistically were significant difference (p < 0.05). The highest hatching was from treatment of 1.5 ml/kg food (R2) and the lowest mortality of embryos was from 0.75 ml/kg food treated one (R1). Analysis of supporting data (egg width, DOD weight, DOD quality) results in no significant different (p > 0.05) among treatments, except for the weight eggs and length eggs data which was highly significance different (p < 0.01) among treatments. Regression-Correlation analysis between fertility rate and egg weight, between fertility rate and hatching rate, between hatching rate and eggs weight, between egg weight and DOD weight data, all shown that no such strong correlation at all (r < 0.06). Embryo survival at late developmental stages and hatching rate datawere significantlu different (p < 0.05) among treatmens. Eggs weight and length were highly significant different (p < 0.01) among treatments, proving that probiotic supplementation through parental feeding determined embryo survival and hatching rate of their egg produced. However, this study also proving that fertility rate and viability of early embryo developmental stage data as well as egg width, DOD weight, DOD quality, had no effect after probiotic treatments for 30 days, need further confirmation that probiotic effectively improving reproductive performance (r < 0,06). Keywords: fertility, embryo viability, probiotics MEP+.

EFEKTIVITAS TETRAPLOIDISASI IKAN NILEM (Osteochilus hasselti Valenciennes 1842) DENGAN KEJUT TEMPERATUR DINGIN 4oC Citra Dina Febrina; Yulia Sistina; Isdy Sulistyo
JURNAL AKUAKULTURA Vol 3, No 2 (2019): Jurnal Akuakultura Universitas Teuku Umar
Publisher : Universitas Teuku Umar

Penelitian ini melaporkan efektivitas tetraploidisasi nilem kejut dingin 4OC pada waktu (15, 20 atau 20 menit) dan lama kejut (10 atau 20 menit) berbeda pada telur nilem terfertilisasi membuktikan bahwa persentase fertilitas telur, persentase penetasan dan persentase kelangsungan hidup antar perlakuan berbeda sangat nyata (P<0,01) sedangkan Hasil pengukuran dimensi sel darah merah benih nilem tetraploid hasil perlakuan membuktikan efektivitas tetraploidisasi kejut dingin yang diperlakukan, yaitu bahwa panjang sel darah merah berbeda nyata (P<0,05) antar perlakuan; lebar sel darah merah berbeda sangat nyata (P<0,01) dengan semua perlakuan; luas dan volume sel darah merah berbeda sangat nyata (P<0,01) dengan semua perlakuan lainnya dipengaruhi oleh perlakuan kejut dingin. Dengan demikian dapat menjadi dasar pengembangan bioteknologi rekayasa reproduksi akuakultur untuk diterapkan dalam skala yang lebih besar dalam rangka memenuhi target peningkatan produksi yang ditetapkan KKP.

Pendampingan Manajemen Usaha dan Kualitas Produksi Usaha Ternak Kambing Kelompok Tani Ternak Mindajaya di Desa Glempang Kecamatan Pekuncen Kabupaten Banyumas Wiwiek Rabiatul Adawiyah; Yulia Sistina; Agustinah Setyaningrum
Darma Sabha Cendekia Vol 3 No 3 (2021): Darma Sabha Cendekia - November 2021
Publisher : Pasca Sarjana | Universitas Jenderal Soedirman

Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan kesejahteraan kelompok masyarakat di desa Glempang Kecamatan Pekuncen Kabupaten Banyumas melalui budidaya kambing. Kelompok Tani Ternak (KTT) Mindajaya beranggotakan sepuluh orang petani di desa Glempang. Permasalahan utama yang dihadapi dari anggota kelompok adalah masih sedikitnya pendapatan yang diterima dari memelihara indukan, karena untuk mendapatkan hasil satu ekor cempe harus menunggu waktu bunting 5 bulan dan menyusui 3 bulan, jadi setelah 8 bulan cempe baru bisa layak jual seharga satu juta jika cempe jantan dan lima ratus ribu jika cempe betina. Pada program penggemukan kambing lima bulan juga belum optimal karena masih mengandalkan rumput atau hijauan yang ada di sekitar hutan Desa Glempang, anggota kelompok belum memiliki ilmu fermentasi pakan ataupun pembuatan silase sehingga setiap hari harus mencari rumput segar dan jika musim penghujan bisa beresiko penyakit cacingan pada kambing ataupun penyakit kembung yang disebabkan oleh pakan atau hijauan yang memiliki kadar air tinggi. Permasalahan lain yang dihadapi adalah: kegiatan usaha dilakukan secara autodidak oleh karena itu perlu dilakukakan penyuluhan terkait sistem reproduksi yang sehat bagi kambing agar bisa memberikan hasil yang lebih maksimal, belum memiliki wawasan tentang menejemen pakan yang baik, kelompok belum memiliki ilmu fermentasi pakan ataupun pembuatan silase, entrepreneurial mindset anggota kelompok masih rendah sehingga belum ada terobosan atau upaya yang sistematis untuk memanfaatan produk derivative untuk menambah penghasilan, kelompok belum memiliki format pembukuan atau laporan keuangan yang sederhana dan mudah digunakan oleh anggota. dan model pemasaran masih bersifat tradisional sehingga hanya mampu mengakses pasar lokal saja. Metode yang digunakan adalah penyuluhan dan pendampingan terkait upaya penyelesaian masalah yang dihadapi oleh kelompok dalam pengembangan usaha ke depan. Pada bulan keenam dilakukan evaluasi terkait pelaksanaan kegiatan untuk mendapatkan umpan balik terkait efektifitas kegiatan. Hasil kegiatan ini mampu meningkatkan pendapatan anggota kelompok melalui manajemen usaha yang profesional dan peningkatan kualitas produksi

Co-Authors Agustinah Setyaningrum Asrul Sahri Siregar Chelsy Rirgiyensi Citra Dina Febrina Farida Nur Rachmawati Farida Nur Rachmawati Isdy Sulistyo Ismoyowati Ismoyowati norman arie prayogo pudji astuti Reni Apriyani RIDWAN AFFANDI Suhestri Suryaningsih Wiwiek Rabiatul Adawiyah Yunita Rusidah

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