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All Journal JFMR (Journal of Fisheries and Marine Research) IJEMS (Indonesian Journal of Environmental Management and Sustainability) Unisia
Gunawan Widi Santosa
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Public Health Social Sciences

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Potential Application of Consortium Microbe from Sea Cucumber Intestinal Symbiont as Preservatives for Vaname Shrimp Marwa Irfan Hanif; Delianis Pringgenies; Gunawan Widi Santosa
Indonesian Journal of Environmental Management and Sustainability Vol. 3 No. 3 (2019): September
Publisher : Research Centre of Inorganic Materials and Complexs

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.557 KB) | DOI: 10.26554/ijems.2019.3.3.93-99

Abstract

Vaname shrimp is one of the most profitable export commodities in Indonesia. However, the shrimp meat undergoes rapid quality degradation when shipped without any preservatives. This is an issue since the preservatives commonly found on the market are formalin-based. This study aims to discover a natural preservative solution by utilizing microbes. The objective of the study is to discover natural preservatives made of symbiont microbe in sea cucumber's intestinal organ by antimicrobial activity screening. Sea cucumber samples were collected from Bandengan waters of Jepara. There are 3 symbiotic microbe form intestinal cucumber that can inhibit the growth from Bacillus cereus and Pseudomonas aeruginosa. These three microbes are Listeria sp., Staphylococcus sp., and Rothia sp. Consequently, tested microbial samples were prepared into a consortium microbe and were tested further as a preservative agent for shrimp with a positive control parameter (cooling). The observations conducted in this study include organoleptic properties, acidity, total colony, proximate test (protein, water, ash, fat, and carbohydrate contents), and Total Volatile Base-Nitrogen (TVBN). The results found 3 active isolates are synergic one to each other as a bacterial consortium. Acidity test of sample extract treatment measured a pH of 7.44, compared to the non-treatment result of 7.14. Organoleptic test results of the shrimp indicated that the shrimp was acceptable for consumption. Proximate test of the treatment did not show a significant difference compared to the positive control treatment. Total colony and TVBN test on treated samples resulted in 48 x 105 CFU/ml and 39.62 mgN% respectively, whereas a similar test on non-treatment sample showed 119 x 105 CFU/ml and 45.31 mgN% respectively. It was concluded that the extract of sea cucumber symbiont microbe consortium showed potency in preventing meat quality degradation in shrimp, although treatment by freezing still produces a better result.
EXPLORATION OF SEA CUCUMBER INTESTINAL SYMBIONT MICROBE AS PROBIOTIC MICROBE CANDIDATE IN HEALTHCARE PRODUCTS Delianis Pringgenies; Putri Hutari Girsang; Ervia Yudiati; Gunawan Widi Santosa
JFMR (Journal of Fisheries and Marine Research) Vol 4, No 1 (2020): JFMR VOL 4 NO 1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.393 KB) | DOI: 10.21776/ub.jfmr.2020.004.01.4

Abstract

Natural ingredients from animals and plants generally have potential in pharmaceutical applications. However, there are still relatively little exploration made on the active ingredients from marine life. Sea cucumbers inhabit seabed and are filter feeders, consuming all microorganisms within their vicinity, which opens the prospect of medicinal applications of sea cucumber intestinal symbiont microbes. This study aims to obtain sea cucumber intestinal symbiont microbes with potential pharmaceutical application as probiotic microbe candidate. The study was carried out by collecting samples, microbe isolation, antimicrobial screening, identification of potential probiotic microbe candidate. Characteristics of the microbe were studied by biochemical screening, and molecular identification. The study found 21 microbial isolates from Holothuria atra and 30 microbial isolates from Holothuria leucospilota. Antimicrobial activity screening results against pathogens of Bacillus cereus dan Pseudomonas aeruginosa found 13 microbial isolates with positive activity. Four species were identified by molecular identification, namely Bacillus aquimaris, Bacillus maritimus, Bacillus toyonensis, and Virgibacillus chiguensis, whereas the other nine were identified by biochemical screening. Four microbe genus, namely Rothia sp., Listeria sp., Micrococcus sp., and Staphylococcus sp, were found to be the most viable candidate for probiotics. It was concluded that Bacillus sp., Rothia sp., Micrococcus sp., and Staphylococcus sp. Exhibited the most potential as probiotic microbes.
Quo Vadis Potensi Kelautan Indonesia Gunawan Widi Santosa
Unisia No. 57: Tahun XXVIII Triwulan III 2005
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/unisia.vol28.iss57.art4

Abstract

Indonesia has an enourmous potency in marine and fishery resources. However, this potency of living and non-living resources has not been optimally utilized. National development based on marine and fishery sector is a stretegic step forward for national sustainable and justice development. For this purpose it is needed an internal strengten to all sectors such as institution, policy, human resources, and data information so that during exploitation will not face problemss in environmental as well assustainability of the resources.  
Co-Authors Ali Ridlo Delianis Pringgenies Delianis Pringgenies Desy Lasri Ana Ervia Yudiati Hadi Endrawati Khajar Imaniar Marwa Irfan Hanif Putri Hutari Girsang Raditya Ahmad Rifandi Radityo Haris RINI PRAMESTI Sunaryo Sunaryo Wakhid Indra Kusuma Wandha Stephani