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All Journal BULETIN OSEANOGRAFI MARINA Science and Technology Indonesia Jurnal DISPROTEK
RINI PRAMESTI
Program Studi Biosains, Program Pascasarjana, Universitas Sebelas Maret, Surakarta 57126, Jawa Tengah, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Electrical & Electronics Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Other

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Metode Sederhana dan Cepat untuk Skrining Bakteri Asam Laktat Penghasil Bakteriosin (Antimicrobial Peptide) dari Intestinum Ikan dan Udang Subagiyo Subagiyo; Sebastian Margino; Triyanto Triyanto; Ria Azizah Tri Nuraini; Wilis Ari Setyati; Rini Pramesti
Buletin Oseanografi Marina Vol 5, No 2 (2016): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.562 KB) | DOI: 10.14710/buloma.v5i2.15727

Abstract

Kontribusi bakteri asam laktat dan bakteriosin dalam pengembangan keamanan makanan telah lama diketahui. Metode sederhana dan cepat diperlukan untuk menseleksi bakteri asam laktat yang mampu menghasilkan bakteriosin. Dua pendekatan telah dilakukan secara berurutan dalam metode ini. Pendekatan pertama adalah seleksi bakteri asam laktat menggunakan medium MRS agar yang diperkaya dengan CaCO3. CaCO3 digunakan sebagai indikator suatu koloni bakteri mampu menghasilkan asam. Pendekatan kedua adalah melakukan seleksi secara langsung terhadap koloni-koloni bakteri yang tumbuh pada medium seleksi pertama menggunakan teknik overlay. Medium MRS soft agar yang telah diinokulasi dengan bakteri Pediococcus acidilactisi di-overlay-kan ke atas koloni-koloni yang tumbuh di medium seleksi pertama. Zona bening yang terbentuk di sekitar koloni menunjukkan dihasilkannya bakteriosin. Koloni-koloni yang menghasilkan zona bening pada tahap pertama dan kedua selanjutnya diisolasi dan dimurnikan. Isolat ini adalah isolat bakteri penghasil asam dan bakteriosin. Pengujian atas metode ini terbukti efektif untuk program isolasi dan seleksi bakteri asam laktat penghasil bakteriosin dari intestinum ikan dan udang. Kata kunci : metode, skrining, bakteri asam laktat, bakteriosin
AKTIVITAS ANTIOKSI DAN RUMPUT LAUT Acanthophora muscoides (Linnaeus) Bory DARI PANTAI KRAKAL GUNUNG KIDUL YOGYAKARTA Rini Pramesti; Ali Ridlo; Wilis Ari Setyati; Muhammad Zainuddin; Muhamad Rahadian Akbar
Jurnal DISPROTEK Vol 8, No 1 (2017)
Publisher : Universitas Islam Nahdlatul Ulama Jepara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34001/jdpt.v8i1.485

Abstract

ABSTRACT Acanthophora muscoides is one of the red seaweed that is potentially used as a natural antioxidant. This study aims to determine the antioxidant activity of methanol and n-hexane extract, and determine the total phenolic content and levels of pigments (chlorophyll and carotenoid) of fresh samples A. muscoides. Antioxidant activity measured using electron transfer method with DPPH (1,1-diphenyl-2-picrylhidrazyl) as free radicals, while the determination of total phenolic content using the Folin-Ciocalteu reagent with gallic acid as standard and determination of pigment content measured by spectrophotometer at a wavelength of 663 nm, 646 nm and 470 nm. The result showed IC50 value of methanol extract was 325.47 ppm and n-hexane extract was 351.27 ppm which means that A. muscoides have very weak antioxidant activity. Total phenolic content in each extract were 22.68 and 46.19 (mg GAE/g extract), chlorophyll a 7.72 and 24.93 (mg/g sample) and carotenoid 28.52 and 68.55 (μ mol/g sample). Keywords: Acanthophora muscoides, antioxidant, free, radical ABSTRAK Acanthophora muscoides merupakan salah satu jenis rumput laut merah dan mempunyai senyawa bioaktif yang dapat digunakan sebagai antioksidan alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak metanol dan n-heksan, kadar total fenolat serta kadar pigmen (klorofil a & karotenoid) dari sampel segar A. muscoides. Penentuan aktivitas antioksidan menggunakan menggunakan metode transfer elektron dengan DPPH (1,1-diphenyl-2-picrylhidrazyl) sebagai radikal bebas. Penentuan kadar total fenolat menggunakan pelarut Folin-Ciocalteu dengan asam galat sebagai standar dan penentuan kandungan pigmen menggunakan prinsip spektofotometri pada λ 663 nm, 646 nm dan 470 nm. Hasil penelitian menunjukkan A. muscoides memiliki aktivitas antioksidan sangat lemah dengan nilai IC50 ekstrak metanol 325,47 ppm dan ekstrak n-heksan 351,27 ppm sedangkan kadar total fenolat pada masing-masing ekstrak 22,68 dan 46,19 (mg GAE/g ekstrak), kadar klorofil a 7,72 dan 24,93 (mg/g sampel), dan kadar karotenoid 28,52 dan 68,55 (µ mol/g sampel). Kata Kunci : Acanthophora muscoides, Antioksidan, Radikal Bebas
Effectiveness of Herbal Extract (Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza) as Immunomodulator in Non-Specific Immunity System of Tiger Grouper (Epinephelus fuscoguttatus) against Infection from Vibrio alginolyticus and Vibrio parah wilis setyati; Subagiyo Subagiyo; Rini Pramesti; Delianis Pringgenies
Science and Technology Indonesia Vol. 4 No. 4 (2019): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (963.639 KB) | DOI: 10.26554/sti.2019.4.4.94-100

Abstract

The success of aquaculture is one of the supporting factors in food sustainability, one of such is the aquaculture of tiger grouper (Epinephelus fuscoguttatus). The main issue of tiger grouper (Epinephelus fuscoguttatus) aquaculture is disease infestation caused by viruses, microbes, and fungi (1). One of the technologies to control such infestation is the immunostimulant of non-specific immunity. Traditional herbs such as Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza have been proven to enhance the immunity system. This study aims to determine the optimal dosage of herb extract (Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza) to strengthen non-specific immunity of tiger grouper (Epinephelus fuscoguttatus), to record data on total leukocyte count and phagocytosis activity in the subject against pathogenic infection from Vibrio alginolyticus and Vibrio parahaemolyticus, and on the side effects of the application of the herbal extracts on meat quality (organoleptic) of tiger grouper (Epinephelus fuscoguttatus)(2). The study was carried out by experimental method using random design with 3 repetitions. The experiment was performed in plastic tanks using flow through system with siphon construction. Steps performed include: Herb sample collection, herb samples extraction, herb extract supplementation into artificial food, dose optimization of herb extract application, challenge test, and side effect test (3). The result shows that treatment with 1% Piper retrofractum, 0.5% Curcuma aeruginosa, and 1% Curcuma zanthorrhiza herb extract increased total leukocyte count and phagocytosis activity in tiger grouper (Epinephelus fuscoguttatus). Organoleptic test of all three treatments indicated similar results, with solid texture and bland flavor. Challenge test against pathogenic infection from V. alginolyticus and V. parahaemolyticus found that the treatment reduced the mortality rate to 0%. Based on the results of this study, Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza were proven to be viable immunomodulator on non-specific immunity system in tiger grouper (Epinephelus fuscoguttatus) fish against infection from Vibrio alginolyticus and Vibrio parahaemolyticus.
Co-Authors A. B. Susanto AB SUSANTO Ali Ridlo Delianis Pringgenies Faishal Islami Galih S. Adiguna Gunawan Widi Santosa Muhamad Rahadian Akbar Muhammad Zainuddin Nirwani Soenardjo Ria Azizah Tri Nuraini Satria Sakti Budi Leksono Sebastian Margino Setia Devi Kurniasih Subagiyo Subagiyo Subagiyo Subagiyo Triyanto Triyanto Wahid Agung Saputra Wakhid Indra Kusuma Wilis Ari Setyati wilis setyati